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The map label for this gene is potH [H]

Identifier: 13475795

GI number: 13475795

Start: 5725740

End: 5726597

Strand: Direct

Name: potH [H]

Synonym: mlr6966

Alternate gene names: 13475795

Gene position: 5725740-5726597 (Clockwise)

Preceding gene: 13475794

Following gene: 13475796

Centisome position: 81.38

GC content: 55.01

Gene sequence:

>858_bases
TTGATCAGCCCCGCGGTTCTGGTCGTCATAATCGGTGCGATCGCACCCCTTCTGGTTATTGTGACGTATTCATTCTTGAC
CCCTGGTCCGACCGGCGGCGTGAACTACACTCTATCTTTCGATGCTTGGACGCGCGTCCTTTTTGACCGCGATGTCTTCG
ACGACACGCTGACGCTGTCAGGGACGAACCTCGAGATTTTGCTGCGCTCGCTTCTCCTGTCGCTGGCAACCACGGTTCTG
TGCCTGCTCGTGGGATTTCCCACCGCCTATTTCATCGCGACCCGCAATCCGCTCAACCGGGAAGTATGGCTTTTGCTCGT
CACCGTTCCGTTCTGGACAAATCTCCTGATCCGCACGTTCGCCATACAGGATCTGATCCGCAACGAAGGGATCATCAATA
CACTTCTGATCAAGGCTGGTTTTATTTCCGAACCAATACAAATGCTGTTTACGAATTTCGCGATTTGTCTCGGACTTGTC
TACGTCTACCTGCCTCTCATGATCCTGCCTGTCTACGCAAGTCTGGAAAGGCAGGACCGAAGCCTGCTCGAGGCGGCGTC
CGACCTGTACGCTCGTCCAGTCAATGTGCTTCGCGAAATCATCGTACCACTTGCCAAGCCCGGCATCATCGCCGGCTCCA
TTCTTGTGTTCATCCCTGCAATCGGAACCTATGTCACTCCTATCCTGCTCGGTGGCGGCAGAAATCTTATGATTGGCAAC
TACATCGAATTTCAGTTCGGCCAAGGTCGCGATTGGCCCTTCGGCTCCGCGCTTTCGCTGGCCCTGATGGCCATCGTGAT
GGTGGCGCTTCTCGTCTACATCCGGCATGGCGACAGCCACGGACACGAACATGGCTAA

Upstream 100 bases:

>100_bases
TCACACCGAACTCAGCCCAGATCCTGCCCGGTTGAGAGGTGTAGAGATGGCAAGTCTTGAAGCAGTCCGGTCAAAATCAG
CAAGCCGAACGCGATGGCTT

Downstream 100 bases:

>100_bases
AGCGTTCCCGGTAACCCACCAGCGCGGCTTCGGCACTGTCGCCATCACAACGTTCATTTTCCTCTACGCACCATTGGCAG
TCGCGCTCGTTTTTGCGTTC

Product: ABC transporter, permease protein

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 285; Mature: 285

Protein sequence:

>285_residues
MISPAVLVVIIGAIAPLLVIVTYSFLTPGPTGGVNYTLSFDAWTRVLFDRDVFDDTLTLSGTNLEILLRSLLLSLATTVL
CLLVGFPTAYFIATRNPLNREVWLLLVTVPFWTNLLIRTFAIQDLIRNEGIINTLLIKAGFISEPIQMLFTNFAICLGLV
YVYLPLMILPVYASLERQDRSLLEAASDLYARPVNVLREIIVPLAKPGIIAGSILVFIPAIGTYVTPILLGGGRNLMIGN
YIEFQFGQGRDWPFGSALSLALMAIVMVALLVYIRHGDSHGHEHG

Sequences:

>Translated_285_residues
MISPAVLVVIIGAIAPLLVIVTYSFLTPGPTGGVNYTLSFDAWTRVLFDRDVFDDTLTLSGTNLEILLRSLLLSLATTVL
CLLVGFPTAYFIATRNPLNREVWLLLVTVPFWTNLLIRTFAIQDLIRNEGIINTLLIKAGFISEPIQMLFTNFAICLGLV
YVYLPLMILPVYASLERQDRSLLEAASDLYARPVNVLREIIVPLAKPGIIAGSILVFIPAIGTYVTPILLGGGRNLMIGN
YIEFQFGQGRDWPFGSALSLALMAIVMVALLVYIRHGDSHGHEHG
>Mature_285_residues
MISPAVLVVIIGAIAPLLVIVTYSFLTPGPTGGVNYTLSFDAWTRVLFDRDVFDDTLTLSGTNLEILLRSLLLSLATTVL
CLLVGFPTAYFIATRNPLNREVWLLLVTVPFWTNLLIRTFAIQDLIRNEGIINTLLIKAGFISEPIQMLFTNFAICLGLV
YVYLPLMILPVYASLERQDRSLLEAASDLYARPVNVLREIIVPLAKPGIIAGSILVFIPAIGTYVTPILLGGGRNLMIGN
YIEFQFGQGRDWPFGSALSLALMAIVMVALLVYIRHGDSHGHEHG

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787080, Length=218, Percent_Identity=44.0366972477064, Blast_Score=196, Evalue=2e-51,
Organism=Escherichia coli, GI1787369, Length=260, Percent_Identity=42.3076923076923, Blast_Score=195, Evalue=3e-51,
Organism=Escherichia coli, GI1787713, Length=230, Percent_Identity=31.304347826087, Blast_Score=107, Evalue=8e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 31331; Mature: 31331

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISPAVLVVIIGAIAPLLVIVTYSFLTPGPTGGVNYTLSFDAWTRVLFDRDVFDDTLTLS
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEHHHHHHHHHHHHHCCCCEEEC
GTNLEILLRSLLLSLATTVLCLLVGFPTAYFIATRNPLNREVWLLLVTVPFWTNLLIRTF
CCCHHHHHHHHHHHHHHHHHHHHHHCCHHEEEEECCCCCCCCEEEEEHHHHHHHHHHHHH
AIQDLIRNEGIINTLLIKAGFISEPIQMLFTNFAICLGLVYVYLPLMILPVYASLERQDR
HHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLLEAASDLYARPVNVLREIIVPLAKPGIIAGSILVFIPAIGTYVTPILLGGGRNLMIGN
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECC
YIEFQFGQGRDWPFGSALSLALMAIVMVALLVYIRHGDSHGHEHG
EEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MISPAVLVVIIGAIAPLLVIVTYSFLTPGPTGGVNYTLSFDAWTRVLFDRDVFDDTLTLS
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEHHHHHHHHHHHHHCCCCEEEC
GTNLEILLRSLLLSLATTVLCLLVGFPTAYFIATRNPLNREVWLLLVTVPFWTNLLIRTF
CCCHHHHHHHHHHHHHHHHHHHHHHCCHHEEEEECCCCCCCCEEEEEHHHHHHHHHHHHH
AIQDLIRNEGIINTLLIKAGFISEPIQMLFTNFAICLGLVYVYLPLMILPVYASLERQDR
HHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLLEAASDLYARPVNVLREIIVPLAKPGIIAGSILVFIPAIGTYVTPILLGGGRNLMIGN
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECC
YIEFQFGQGRDWPFGSALSLALMAIVMVALLVYIRHGDSHGHEHG
EEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503 [H]