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The map label for this gene is glcD [H]

Identifier: 13475757

GI number: 13475757

Start: 5686897

End: 5688324

Strand: Direct

Name: glcD [H]

Synonym: mlr6916

Alternate gene names: 13475757

Gene position: 5686897-5688324 (Clockwise)

Preceding gene: 13475756

Following gene: 13475758

Centisome position: 80.82

GC content: 64.08

Gene sequence:

>1428_bases
ATGCGCATCATCGTGCCGGGCGAGGGCGTGGTCGACGCGGCCAATGCCATGCGCGCCTTCGAGAGCGACGGCCTGACCGC
CTATCGGCAATTGCCGCTGGTCGTCGTTCTGCCCGAGACCGTGGCGCAGGTCTCCCGCGTGCTGAAATACTGCAACGACC
GCAACATCCGCGTCGTGCCGCGCGGTTCCGGTACCTCGCTGTCCGGCGGTGCGCTGCCGCTCGAGGACGCGGTGCTTCTG
GTGATGAGCCGTTTCAACCGCATCCTGGCGATCGATTTTCCCAACCGCATCGTCGTCGCCCAGCCGGGCGTCACCAATCT
AGGCATCACCACCGCCGTCGAGCAGGAGGGCTTTTATTACGCCCCCGATCCATCCTCCCAGATCGCCTGCTCGATCGGCG
GCAATGTCGCGGAGAATTCCGGCGGCGTGCATTGCCTGAAATACGGCCTCACCGCCAACAATGTGCTCGGCATCGAGATG
GTGCTGATGAATGGCGAGGTGGTGCGGCTCGGCGGCAGCCATCTCGATGCCGAAGGCTACGATCTGCTTGGCGTCATGAC
CGGTTCGGAAGGTCTGCTCGGCGTCGTCACCGAGGTTACGGTCCGCATCTTGAAGAAGCCGGAGACGGCACGCGCCCTGC
TGATCGGCTTTCCGACCAGCGAGCAGGGCGGCCAGTGCGTCGCCGACATCATCGGCGCCGGCATCATCCCCGGCGGCATG
GAAATGATGGACCGGCCGGCGATCCACGCTGCGGAGGATTTCGTCCATGCCGGCTATCCCCTCGATGTCGAGGCGTTGCT
GATCGTCGAGCTCGACGGGCCGGGAGTCGAGGTCGATCACCTCATCACCGCGGTCGAGGCGATCGCCATAAGCAACGGCT
CGACCACCTGCCGCATCTCGCAATCCGAGGCGGAGCGGCTGAGCTTCTGGGCCGGCCGCAAGGCGGCATTCCCGGCGGTC
GGCCGCATTTCGCCCGACTATTACTGCATGGACGGCACCATCCCCCGCAAGGAACTGCCGCGCGTGCTCGCCGGCATGCG
CGAACTGTCGGAGAAATACGGTCTCGGCGTCGCCAATGTCTTTCACGCCGGCGACGGCAATCTGCATCCGCTGATCCTCT
ACGATGCCAATGTTCCCGGCGAACTCGACAAGGCCGAAAGTTTTGGCGCCGACATATTGCGCCTTTGTGTCAAGGTCGGC
GGCGTGCTGACGGGCGAACATGGGGTTGGGGTCGAGAAGCGCGATCTGATGCCGGAAATGTTCAACCAGGTCGATCTCGA
CCAGCAGATGCGCATCAAATGCGCCTTCGACCCCAACCACCTGCTCAACCCGGGAAAAGTCTTCCCGCAACTGCGCCGCT
GCGCCGAGCTTGGGCGCATGCATGTGCACCGGGGTCAGGTGGCATTTCCGGACATTCCGAGGTTTTGA

Upstream 100 bases:

>100_bases
TTTTGACCAGTGAAGCGAAACGAGGCTGCTGATGTCCGGCCTGGCCATGCCGAAACCAGACGACGCCACGATGCGCCGGC
GCGGCGAGATCGTCGCCGAC

Downstream 100 bases:

>100_bases
GGGGATGCTGTTCCGGGACCAGACCCTTGCGGCCATTGCGTCGGGCGAAGTGACGCTTGCCTTTCGCCGCCGGAAGCGGC
CGACGGTTAAGGCTGGCGGG

Product: glycolate oxidase subunit GlcD

Products: 2-oxo acid; H2O2

Alternate protein names: NA

Number of amino acids: Translated: 475; Mature: 475

Protein sequence:

>475_residues
MRIIVPGEGVVDAANAMRAFESDGLTAYRQLPLVVVLPETVAQVSRVLKYCNDRNIRVVPRGSGTSLSGGALPLEDAVLL
VMSRFNRILAIDFPNRIVVAQPGVTNLGITTAVEQEGFYYAPDPSSQIACSIGGNVAENSGGVHCLKYGLTANNVLGIEM
VLMNGEVVRLGGSHLDAEGYDLLGVMTGSEGLLGVVTEVTVRILKKPETARALLIGFPTSEQGGQCVADIIGAGIIPGGM
EMMDRPAIHAAEDFVHAGYPLDVEALLIVELDGPGVEVDHLITAVEAIAISNGSTTCRISQSEAERLSFWAGRKAAFPAV
GRISPDYYCMDGTIPRKELPRVLAGMRELSEKYGLGVANVFHAGDGNLHPLILYDANVPGELDKAESFGADILRLCVKVG
GVLTGEHGVGVEKRDLMPEMFNQVDLDQQMRIKCAFDPNHLLNPGKVFPQLRRCAELGRMHVHRGQVAFPDIPRF

Sequences:

>Translated_475_residues
MRIIVPGEGVVDAANAMRAFESDGLTAYRQLPLVVVLPETVAQVSRVLKYCNDRNIRVVPRGSGTSLSGGALPLEDAVLL
VMSRFNRILAIDFPNRIVVAQPGVTNLGITTAVEQEGFYYAPDPSSQIACSIGGNVAENSGGVHCLKYGLTANNVLGIEM
VLMNGEVVRLGGSHLDAEGYDLLGVMTGSEGLLGVVTEVTVRILKKPETARALLIGFPTSEQGGQCVADIIGAGIIPGGM
EMMDRPAIHAAEDFVHAGYPLDVEALLIVELDGPGVEVDHLITAVEAIAISNGSTTCRISQSEAERLSFWAGRKAAFPAV
GRISPDYYCMDGTIPRKELPRVLAGMRELSEKYGLGVANVFHAGDGNLHPLILYDANVPGELDKAESFGADILRLCVKVG
GVLTGEHGVGVEKRDLMPEMFNQVDLDQQMRIKCAFDPNHLLNPGKVFPQLRRCAELGRMHVHRGQVAFPDIPRF
>Mature_475_residues
MRIIVPGEGVVDAANAMRAFESDGLTAYRQLPLVVVLPETVAQVSRVLKYCNDRNIRVVPRGSGTSLSGGALPLEDAVLL
VMSRFNRILAIDFPNRIVVAQPGVTNLGITTAVEQEGFYYAPDPSSQIACSIGGNVAENSGGVHCLKYGLTANNVLGIEM
VLMNGEVVRLGGSHLDAEGYDLLGVMTGSEGLLGVVTEVTVRILKKPETARALLIGFPTSEQGGQCVADIIGAGIIPGGM
EMMDRPAIHAAEDFVHAGYPLDVEALLIVELDGPGVEVDHLITAVEAIAISNGSTTCRISQSEAERLSFWAGRKAAFPAV
GRISPDYYCMDGTIPRKELPRVLAGMRELSEKYGLGVANVFHAGDGNLHPLILYDANVPGELDKAESFGADILRLCVKVG
GVLTGEHGVGVEKRDLMPEMFNQVDLDQQMRIKCAFDPNHLLNPGKVFPQLRRCAELGRMHVHRGQVAFPDIPRF

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding PCMH-type domain [H]

Homologues:

Organism=Homo sapiens, GI37595756, Length=454, Percent_Identity=28.8546255506608, Blast_Score=205, Evalue=8e-53,
Organism=Homo sapiens, GI37595754, Length=477, Percent_Identity=27.6729559748428, Blast_Score=197, Evalue=2e-50,
Organism=Homo sapiens, GI119964728, Length=452, Percent_Identity=30.3097345132743, Blast_Score=181, Evalue=1e-45,
Organism=Homo sapiens, GI4501993, Length=250, Percent_Identity=27.6, Blast_Score=99, Evalue=1e-20,
Organism=Escherichia coli, GI1789351, Length=475, Percent_Identity=57.8947368421053, Blast_Score=550, Evalue=1e-158,
Organism=Escherichia coli, GI1787977, Length=504, Percent_Identity=25.7936507936508, Blast_Score=122, Evalue=4e-29,
Organism=Escherichia coli, GI48994907, Length=445, Percent_Identity=26.5168539325843, Blast_Score=100, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI71992373, Length=459, Percent_Identity=29.1938997821351, Blast_Score=197, Evalue=1e-50,
Organism=Caenorhabditis elegans, GI71992381, Length=459, Percent_Identity=29.6296296296296, Blast_Score=185, Evalue=5e-47,
Organism=Caenorhabditis elegans, GI71992392, Length=363, Percent_Identity=28.6501377410468, Blast_Score=159, Evalue=3e-39,
Organism=Caenorhabditis elegans, GI17534361, Length=426, Percent_Identity=26.5258215962441, Blast_Score=132, Evalue=5e-31,
Organism=Caenorhabditis elegans, GI17556096, Length=266, Percent_Identity=25.9398496240602, Blast_Score=91, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6320027, Length=458, Percent_Identity=27.9475982532751, Blast_Score=199, Evalue=6e-52,
Organism=Saccharomyces cerevisiae, GI6320023, Length=452, Percent_Identity=28.0973451327434, Blast_Score=171, Evalue=3e-43,
Organism=Saccharomyces cerevisiae, GI6320764, Length=474, Percent_Identity=26.5822784810127, Blast_Score=161, Evalue=2e-40,
Organism=Drosophila melanogaster, GI18921117, Length=430, Percent_Identity=28.8372093023256, Blast_Score=157, Evalue=1e-38,
Organism=Drosophila melanogaster, GI24639277, Length=430, Percent_Identity=28.8372093023256, Blast_Score=157, Evalue=1e-38,
Organism=Drosophila melanogaster, GI24639275, Length=430, Percent_Identity=28.8372093023256, Blast_Score=157, Evalue=1e-38,
Organism=Drosophila melanogaster, GI24653753, Length=221, Percent_Identity=28.5067873303167, Blast_Score=95, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016166
- InterPro:   IPR016167
- InterPro:   IPR016164
- InterPro:   IPR016168
- InterPro:   IPR004113
- InterPro:   IPR004490
- InterPro:   IPR006094
- InterPro:   IPR016171 [H]

Pfam domain/function: PF02913 FAD-oxidase_C; PF01565 FAD_binding_4 [H]

EC number: 1.1.3.15

Molecular weight: Translated: 50915; Mature: 50915

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIIVPGEGVVDAANAMRAFESDGLTAYRQLPLVVVLPETVAQVSRVLKYCNDRNIRVVP
CEEEECCCCCHHHHHHHHHHHCCCCEEHHHCCEEEECCHHHHHHHHHHHHHCCCCEEEEE
RGSGTSLSGGALPLEDAVLLVMSRFNRILAIDFPNRIVVAQPGVTNLGITTAVEQEGFYY
CCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEECCCEEEEECCCCCCCCEEEEEECCCCEE
APDPSSQIACSIGGNVAENSGGVHCLKYGLTANNVLGIEMVLMNGEVVRLGGSHLDAEGY
CCCCCCCEEEEECCCCCCCCCCEEEEEECCCCCCEEEEEEEEECCEEEEECCCCCCCCCC
DLLGVMTGSEGLLGVVTEVTVRILKKPETARALLIGFPTSEQGGQCVADIIGAGIIPGGM
EEEEEEECCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHHHHHCCCCCCCCH
EMMDRPAIHAAEDFVHAGYPLDVEALLIVELDGPGVEVDHLITAVEAIAISNGSTTCRIS
HHHCCCCHHHHHHHHHCCCCCCEEEEEEEEECCCCCCHHHHHHHHHHEEECCCCCEEEEE
QSEAERLSFWAGRKAAFPAVGRISPDYYCMDGTIPRKELPRVLAGMRELSEKYGLGVANV
HHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCHHHE
FHAGDGNLHPLILYDANVPGELDKAESFGADILRLCVKVGGVLTGEHGVGVEKRDLMPEM
EECCCCCCCEEEEECCCCCCCCCHHHHCCHHHHHHHHHHCCEEECCCCCCCCHHHHHHHH
FNQVDLDQQMRIKCAFDPNHLLNPGKVFPQLRRCAELGRMHVHRGQVAFPDIPRF
HHHCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHEECCCCCCCCCCCCC
>Mature Secondary Structure
MRIIVPGEGVVDAANAMRAFESDGLTAYRQLPLVVVLPETVAQVSRVLKYCNDRNIRVVP
CEEEECCCCCHHHHHHHHHHHCCCCEEHHHCCEEEECCHHHHHHHHHHHHHCCCCEEEEE
RGSGTSLSGGALPLEDAVLLVMSRFNRILAIDFPNRIVVAQPGVTNLGITTAVEQEGFYY
CCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEECCCEEEEECCCCCCCCEEEEEECCCCEE
APDPSSQIACSIGGNVAENSGGVHCLKYGLTANNVLGIEMVLMNGEVVRLGGSHLDAEGY
CCCCCCCEEEEECCCCCCCCCCEEEEEECCCCCCEEEEEEEEECCEEEEECCCCCCCCCC
DLLGVMTGSEGLLGVVTEVTVRILKKPETARALLIGFPTSEQGGQCVADIIGAGIIPGGM
EEEEEEECCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHHHHHCCCCCCCCH
EMMDRPAIHAAEDFVHAGYPLDVEALLIVELDGPGVEVDHLITAVEAIAISNGSTTCRIS
HHHCCCCHHHHHHHHHCCCCCCEEEEEEEEECCCCCCHHHHHHHHHHEEECCCCCEEEEE
QSEAERLSFWAGRKAAFPAVGRISPDYYCMDGTIPRKELPRVLAGMRELSEKYGLGVANV
HHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCHHHE
FHAGDGNLHPLILYDANVPGELDKAESFGADILRLCVKVGGVLTGEHGVGVEKRDLMPEM
EECCCCCCCEEEEECCCCCCCCCHHHHCCHHHHHHHHHHCCEEECCCCCCCCHHHHHHHH
FNQVDLDQQMRIKCAFDPNHLLNPGKVFPQLRRCAELGRMHVHRGQVAFPDIPRF
HHHCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (S)-2-hydroxy acid; O2

Specific reaction: (S)-2-hydroxy acid + O2 = 2-oxo acid + H2O2

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]