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The map label for this gene is asnB [H]

Identifier: 13475635

GI number: 13475635

Start: 5550471

End: 5552468

Strand: Direct

Name: asnB [H]

Synonym: mlr6755

Alternate gene names: 13475635

Gene position: 5550471-5552468 (Clockwise)

Preceding gene: 13475634

Following gene: 13475636

Centisome position: 78.89

GC content: 63.71

Gene sequence:

>1998_bases
ATGTGTGGGATCGCCGGAATTCTCTTGGCGCCCGATGCAGCCAATACCAACGCGCTCAGGGCGATCGGGCCGATGATGAT
GGCCCTTCGTCATCGCGGGCCTGATGGCGAGGCATTCTGGATGAGCCGTGAAGCCGGGATCGCCTTCGGGCACCGGCGTC
TGGCCATCGTCGACCTGTCGGAGGCGGGTCGCCAGCCGATGCACTCCGCGAGCGGCCGGTATGTCATTACCTTCAATGGC
GAAATATACAATTTTCGTGATCTGCGCCGTGAACTGGAAGGGGCGGGCCACCATTTCCGCGGCACCAGCGACACGGAAGT
GATGCTGTGCGCGATCGAGAGCTGGGGCCTCGAGGCGGCACTTGCGCGCTTCGCCGGCATGTTCGCTTTCGGGCTATGGG
ATCTGAAGAACCGGACTCTCCACCTCGCCCGCGACCGGATGGGCAAAAAGCCGCTCTATGTCGCCTCGACACCTGAAGCG
CTTGTCTTCGCCTCGGAGCTGAAGGCGATCACTTGCTTCCCGGGATTCACCCCGGAACTCGATGTCGATGCGGCGGCGAC
GATGCTTTCGAAAGGGTGGGTGCCGGATGACCGTTGCATCTGGCAGGGCGTGTTCAAGCTGCCGCCCGGTTCAGTCCTGT
CGGTGACGGCGGCGGATTTCGCCAACGCCCGCGGCGCCGGCTCGCTTGCGCATCGCATTCGCCGCTGGTGGTCGCTGGCC
GATGTCGCCTGCAAGGCGCAGCGGGATCCGATCGCCGGCAGCGACGAGGACCTTACGACCGAACTCGACAGCCTGCTTCG
CCTTGCCGTCAAAGAACGAATGGTTGCCGATGTGCCGCTTGGCGCCTTTCTGTCGGGTGGAATTGACAGCTCGACGGTCG
TCGCCCTGATGCAGGCGCAGTCCCCGACGCCCGTTCGCACGTTCACCATAGCGTTCGGCGAGAGCGGGTTCGACGAGGCC
CCTCATGCGGCCGCGGTTGCGCGGCATCTGGGCACCGACCATACGGAACTTCATCTTTCACCGGCCGCGGCGCTTGAGGT
CATTCCGGAGTTGCCGAGGATCTGGGACGAGCCGTTCGCCGACGAGTCGCAGATACCGACGCTGCTGGTGTCGCGCCTCG
CCCGGCGACACGTCACCGTGGCTCTGTCGGGCGATGGCGGCGACGAGTGCTTCGCGGGCTACTCTCGACACTTCCTGGCA
ACCCGCCTCAGAAGGCAGCATGAGCTGCCGTCGTCGTTTCGGCGGATGTTGGCGGCGGGGGCAGGATTGCTGGCCCAGGC
CTCCCAGAAAGACATCTTCGGCAGCCTGCCGCTTTCGGCGAACATGCGGCACGGATTGCGCGGTGACCGGTTGAACCGCC
TCGCCCGCCTGTTCGCGGCGGCAAATGAGGACCAACTGCTTCAGCGGCTGACGGAATCTTCGACCAACAAGCTTATTCAT
CACAAGCCAGCGGCATCCAATGCCAGCGCCACACAGCTCGACGGCCTGCTGTCGCGCCTCCTGTTCGACGACATGACCGG
CTATCTCCCCGGCGACATCCTGGTGAAGCTCGATCGTGCCACCATGGCCAACAGCCTCGAGGGGCGATGCCCGATCCTGG
ACCATCGCGTCGTTGAATTCGCCTGGCGACTGCCAACCGATGCCAAGGTCCGAAGTGGCAAGGGCAAATGGATACTGCGC
CAGCTCCTTGATCGTTATGTACCGCGCCGACTGGTCGATCGGCCCAAGCAGGGTTTCGACGTTCCGGTCGGAGCCTGGCT
GAGAGGACCCCTGCGCGACTGGGCAACGGATATCATTGCCACGACACGCCTGTCCGGAGATGGGGTCATCGACTGTGCGA
AGGTCGACGCATGTTGGCGCGATCATCTGCATGGAAACCAGGATCATTTCCGTGACTTGTGGCCGCTGCTGATGTTCCAG
GCATGGCGCAACGACGCCATGCGGCCATCGGCGCCGGCAACGCACGCTTCTTACGATATCGAACTGACAGGGGATTGA

Upstream 100 bases:

>100_bases
ATCATCCGCCATCCGATCGTGTGGTTCTGTCTGACCCTCTGCCTTGTCGCCGGAACGCCGCAGACGGTCCCTGCGATCGT
TCGCCAGAGGAGATATTAGA

Downstream 100 bases:

>100_bases
AATGGAAGGTTCCATAGCCAGCCGCGCCAGGCTTCCGCGTGCTACGCAGATGGCCGTCGCTACGCTCGACACCAATCCGA
GGATGAGGCGACCGCCGCGC

Product: asparagine synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 665; Mature: 665

Protein sequence:

>665_residues
MCGIAGILLAPDAANTNALRAIGPMMMALRHRGPDGEAFWMSREAGIAFGHRRLAIVDLSEAGRQPMHSASGRYVITFNG
EIYNFRDLRRELEGAGHHFRGTSDTEVMLCAIESWGLEAALARFAGMFAFGLWDLKNRTLHLARDRMGKKPLYVASTPEA
LVFASELKAITCFPGFTPELDVDAAATMLSKGWVPDDRCIWQGVFKLPPGSVLSVTAADFANARGAGSLAHRIRRWWSLA
DVACKAQRDPIAGSDEDLTTELDSLLRLAVKERMVADVPLGAFLSGGIDSSTVVALMQAQSPTPVRTFTIAFGESGFDEA
PHAAAVARHLGTDHTELHLSPAAALEVIPELPRIWDEPFADESQIPTLLVSRLARRHVTVALSGDGGDECFAGYSRHFLA
TRLRRQHELPSSFRRMLAAGAGLLAQASQKDIFGSLPLSANMRHGLRGDRLNRLARLFAAANEDQLLQRLTESSTNKLIH
HKPAASNASATQLDGLLSRLLFDDMTGYLPGDILVKLDRATMANSLEGRCPILDHRVVEFAWRLPTDAKVRSGKGKWILR
QLLDRYVPRRLVDRPKQGFDVPVGAWLRGPLRDWATDIIATTRLSGDGVIDCAKVDACWRDHLHGNQDHFRDLWPLLMFQ
AWRNDAMRPSAPATHASYDIELTGD

Sequences:

>Translated_665_residues
MCGIAGILLAPDAANTNALRAIGPMMMALRHRGPDGEAFWMSREAGIAFGHRRLAIVDLSEAGRQPMHSASGRYVITFNG
EIYNFRDLRRELEGAGHHFRGTSDTEVMLCAIESWGLEAALARFAGMFAFGLWDLKNRTLHLARDRMGKKPLYVASTPEA
LVFASELKAITCFPGFTPELDVDAAATMLSKGWVPDDRCIWQGVFKLPPGSVLSVTAADFANARGAGSLAHRIRRWWSLA
DVACKAQRDPIAGSDEDLTTELDSLLRLAVKERMVADVPLGAFLSGGIDSSTVVALMQAQSPTPVRTFTIAFGESGFDEA
PHAAAVARHLGTDHTELHLSPAAALEVIPELPRIWDEPFADESQIPTLLVSRLARRHVTVALSGDGGDECFAGYSRHFLA
TRLRRQHELPSSFRRMLAAGAGLLAQASQKDIFGSLPLSANMRHGLRGDRLNRLARLFAAANEDQLLQRLTESSTNKLIH
HKPAASNASATQLDGLLSRLLFDDMTGYLPGDILVKLDRATMANSLEGRCPILDHRVVEFAWRLPTDAKVRSGKGKWILR
QLLDRYVPRRLVDRPKQGFDVPVGAWLRGPLRDWATDIIATTRLSGDGVIDCAKVDACWRDHLHGNQDHFRDLWPLLMFQ
AWRNDAMRPSAPATHASYDIELTGD
>Mature_665_residues
MCGIAGILLAPDAANTNALRAIGPMMMALRHRGPDGEAFWMSREAGIAFGHRRLAIVDLSEAGRQPMHSASGRYVITFNG
EIYNFRDLRRELEGAGHHFRGTSDTEVMLCAIESWGLEAALARFAGMFAFGLWDLKNRTLHLARDRMGKKPLYVASTPEA
LVFASELKAITCFPGFTPELDVDAAATMLSKGWVPDDRCIWQGVFKLPPGSVLSVTAADFANARGAGSLAHRIRRWWSLA
DVACKAQRDPIAGSDEDLTTELDSLLRLAVKERMVADVPLGAFLSGGIDSSTVVALMQAQSPTPVRTFTIAFGESGFDEA
PHAAAVARHLGTDHTELHLSPAAALEVIPELPRIWDEPFADESQIPTLLVSRLARRHVTVALSGDGGDECFAGYSRHFLA
TRLRRQHELPSSFRRMLAAGAGLLAQASQKDIFGSLPLSANMRHGLRGDRLNRLARLFAAANEDQLLQRLTESSTNKLIH
HKPAASNASATQLDGLLSRLLFDDMTGYLPGDILVKLDRATMANSLEGRCPILDHRVVEFAWRLPTDAKVRSGKGKWILR
QLLDRYVPRRLVDRPKQGFDVPVGAWLRGPLRDWATDIIATTRLSGDGVIDCAKVDACWRDHLHGNQDHFRDLWPLLMFQ
AWRNDAMRPSAPATHASYDIELTGD

Specific function: Main asparagine synthetase in vegetative cells [H]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI168229248, Length=417, Percent_Identity=28.7769784172662, Blast_Score=132, Evalue=1e-30,
Organism=Homo sapiens, GI168229252, Length=417, Percent_Identity=28.7769784172662, Blast_Score=132, Evalue=1e-30,
Organism=Homo sapiens, GI168229250, Length=417, Percent_Identity=28.7769784172662, Blast_Score=132, Evalue=1e-30,
Organism=Homo sapiens, GI296010848, Length=391, Percent_Identity=28.9002557544757, Blast_Score=125, Evalue=2e-28,
Organism=Escherichia coli, GI1786889, Length=436, Percent_Identity=30.5045871559633, Blast_Score=142, Evalue=9e-35,
Organism=Saccharomyces cerevisiae, GI6321563, Length=439, Percent_Identity=28.0182232346241, Blast_Score=113, Evalue=8e-26,
Organism=Saccharomyces cerevisiae, GI6325403, Length=445, Percent_Identity=26.9662921348315, Blast_Score=109, Evalue=1e-24,
Organism=Drosophila melanogaster, GI45553209, Length=396, Percent_Identity=28.7878787878788, Blast_Score=126, Evalue=6e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 73159; Mature: 73159

Theoretical pI: Translated: 7.52; Mature: 7.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGIAGILLAPDAANTNALRAIGPMMMALRHRGPDGEAFWMSREAGIAFGHRRLAIVDLS
CCCCCCEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCEECCCEEEEEEHH
EAGRQPMHSASGRYVITFNGEIYNFRDLRRELEGAGHHFRGTSDTEVMLCAIESWGLEAA
HHCCCCCHHCCCCEEEEECCCEECHHHHHHHHHCCCCCCCCCCCCEEEEEEEHHCCHHHH
LARFAGMFAFGLWDLKNRTLHLARDRMGKKPLYVASTPEALVFASELKAITCFPGFTPEL
HHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCEEEECCCHHHHHHHHCCEEEECCCCCCCC
DVDAAATMLSKGWVPDDRCIWQGVFKLPPGSVLSVTAADFANARGAGSLAHRIRRWWSLA
CHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCEEEEEHHHHCCCCCCHHHHHHHHHHHHHH
DVACKAQRDPIAGSDEDLTTELDSLLRLAVKERMVADVPLGAFLSGGIDSSTVVALMQAQ
HHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCHHHHHHHHHCC
SPTPVRTFTIAFGESGFDEAPHAAAVARHLGTDHTELHLSPAAALEVIPELPRIWDEPFA
CCCCEEEEEEEECCCCCCCCCHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHHCCCCC
DESQIPTLLVSRLARRHVTVALSGDGGDECFAGYSRHFLATRLRRQHELPSSFRRMLAAG
CCCCCHHHHHHHHHHCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHC
AGLLAQASQKDIFGSLPLSANMRHGLRGDRLNRLARLFAAANEDQLLQRLTESSTNKLIH
CCHHHHCHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCEEE
HKPAASNASATQLDGLLSRLLFDDMTGYLPGDILVKLDRATMANSLEGRCPILDHRVVEF
CCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEECHHHHHCCCCCCCCCHHHHHHHH
AWRLPTDAKVRSGKGKWILRQLLDRYVPRRLVDRPKQGFDVPVGAWLRGPLRDWATDIIA
HHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCHHHHHHHHHHH
TTRLSGDGVIDCAKVDACWRDHLHGNQDHFRDLWPLLMFQAWRNDAMRPSAPATHASYDI
HEEECCCCCEEHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEE
ELTGD
EECCC
>Mature Secondary Structure
MCGIAGILLAPDAANTNALRAIGPMMMALRHRGPDGEAFWMSREAGIAFGHRRLAIVDLS
CCCCCCEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCEECCCEEEEEEHH
EAGRQPMHSASGRYVITFNGEIYNFRDLRRELEGAGHHFRGTSDTEVMLCAIESWGLEAA
HHCCCCCHHCCCCEEEEECCCEECHHHHHHHHHCCCCCCCCCCCCEEEEEEEHHCCHHHH
LARFAGMFAFGLWDLKNRTLHLARDRMGKKPLYVASTPEALVFASELKAITCFPGFTPEL
HHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCEEEECCCHHHHHHHHCCEEEECCCCCCCC
DVDAAATMLSKGWVPDDRCIWQGVFKLPPGSVLSVTAADFANARGAGSLAHRIRRWWSLA
CHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCEEEEEHHHHCCCCCCHHHHHHHHHHHHHH
DVACKAQRDPIAGSDEDLTTELDSLLRLAVKERMVADVPLGAFLSGGIDSSTVVALMQAQ
HHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCHHHHHHHHHCC
SPTPVRTFTIAFGESGFDEAPHAAAVARHLGTDHTELHLSPAAALEVIPELPRIWDEPFA
CCCCEEEEEEEECCCCCCCCCHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHHCCCCC
DESQIPTLLVSRLARRHVTVALSGDGGDECFAGYSRHFLATRLRRQHELPSSFRRMLAAG
CCCCCHHHHHHHHHHCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHC
AGLLAQASQKDIFGSLPLSANMRHGLRGDRLNRLARLFAAANEDQLLQRLTESSTNKLIH
CCHHHHCHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCEEE
HKPAASNASATQLDGLLSRLLFDDMTGYLPGDILVKLDRATMANSLEGRCPILDHRVVEF
CCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEECHHHHHCCCCCCCCCHHHHHHHH
AWRLPTDAKVRSGKGKWILRQLLDRYVPRRLVDRPKQGFDVPVGAWLRGPLRDWATDIIA
HHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCHHHHHHHHHHH
TTRLSGDGVIDCAKVDACWRDHLHGNQDHFRDLWPLLMFQAWRNDAMRPSAPATHASYDI
HEEECCCCCEEHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEE
ELTGD
EECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377; 8755891; 10498721 [H]