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The map label for this gene is bepF [H]

Identifier: 13475614

GI number: 13475614

Start: 5523979

End: 5525187

Strand: Reverse

Name: bepF [H]

Synonym: mll6731

Alternate gene names: 13475614

Gene position: 5525187-5523979 (Counterclockwise)

Preceding gene: 13475615

Following gene: 13475613

Centisome position: 78.53

GC content: 63.94

Gene sequence:

>1209_bases
ATGAGGGCGCCGTGCGAAAGATCCGAGCGACATCGTTCCGTAGCGAGGCGGCGATGGATCAGTCGCGCTTCTTGGGCTTG
CTTGATGGCTGCCTGCTTGGGGGGCGCCGCACCACCTTTCGTCAGTTCGGTTTCATTCGCCCAGCAGCCTGGTGCAAGGC
AGGCGCCGCCTCCCGCGGTCGTCGTCGAGAAGATCGAGGTTCAGCAGGTCAGCAATCCCGCGCAGTTTACCGGTCGTGTG
GAAGCGATAGAAGCAGTCGACATCCGGGCGCGTGTCACCGGCTTTCTGCACTCCGTCGATTTCAAGGATGGGCAAGCGGT
AAAGGCTGGGGACACGCTGTTTGAAATCGAGCCGGATCAGCTTAATGCGCTGGTCGCTTCGGCGCGGGCGCAGGTGGCGC
GTGCCGACGCAACACGAATATCGGCCGAGCGGAGCCTGGCGCGCAACCGCGACCTGTTGGCCCGAAGAACCGTCTCGCAG
GCCACGGTGGACGAGGTCCAAGCAGCATTCGATGTCGCTTCCGCCGATGTGCAGGTCGCTCAGGCCGCACTGGATACCGC
CGAACTCAACCTGTCCTATGCTCATATCACGGCCCCGATCTCCGGCAGCATTGGCCGCGCGACGTTCACGACGGGCAATC
TTGTCGGACCGGATTCCGGTTCGCTGGCACGGATAGTCAGCCTGGACACCGTCCGCGTCGCTTTCGCCGTGACCGAGGGG
TTGCTTGTGACGATCCGCCAGCAGCAGGCGAGCGGCGGCGGCCTCGATCCGAATACGCTGAAACTCTCGCTGCGTCTTGC
CAACGGGACGGACTATGGCACGCCCGGCCGCATCGAATTTATCGAGAACGAGATCGACCCGCAGACCGGGACGGTCGCGG
CCCGTGCGGTTTTCCCCAATCCTCGTCACATCCTCCTGCCAGGACAGTTCGTCACTCTTTATGTGCAGGAGAAAGACGCC
CCTAGCTTGCCGGTGGTGCCCCAGACAGCGGTGCTGCAGGATCGCGAGGGGCGCTTCGTCTATCTGCTCGGCAAGGACAA
TATCGTCTCGCAGCAGCGCATCCACACAGGCGCACGCGTCGGCAATGGATGGGCGGTCACCAAGGGCCTTGACGGCGGCG
AGCAGGTCGTCGTCGAGGGAATCCAACGTCTCGCCCAGGGCATGACGGTTCAGCCGAGCCAAGGCCAGCCGGTCGGCGGC
GGCACATGA

Upstream 100 bases:

>100_bases
TTACCAGCATTTACACGCCAGGCACGGTTGCCGGCATTTCTCTCCTTGTCGCCGGCGGCGCCTATATGGCCATTGCCCTG
ACGATGCGTAGAGCTCGGCC

Downstream 100 bases:

>100_bases
TTTCCGATCTCTTCATCACCCGCACCCGGCTGGCGATCGTCTTGTCGATCGTCATTTCGATTGCGGGCGCGATCGCCATC
TTTTCGCTGCCGGTGCAGCA

Product: multidrug efflux membrane fusion protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 402; Mature: 402

Protein sequence:

>402_residues
MRAPCERSERHRSVARRRWISRASWACLMAACLGGAAPPFVSSVSFAQQPGARQAPPPAVVVEKIEVQQVSNPAQFTGRV
EAIEAVDIRARVTGFLHSVDFKDGQAVKAGDTLFEIEPDQLNALVASARAQVARADATRISAERSLARNRDLLARRTVSQ
ATVDEVQAAFDVASADVQVAQAALDTAELNLSYAHITAPISGSIGRATFTTGNLVGPDSGSLARIVSLDTVRVAFAVTEG
LLVTIRQQQASGGGLDPNTLKLSLRLANGTDYGTPGRIEFIENEIDPQTGTVAARAVFPNPRHILLPGQFVTLYVQEKDA
PSLPVVPQTAVLQDREGRFVYLLGKDNIVSQQRIHTGARVGNGWAVTKGLDGGEQVVVEGIQRLAQGMTVQPSQGQPVGG
GT

Sequences:

>Translated_402_residues
MRAPCERSERHRSVARRRWISRASWACLMAACLGGAAPPFVSSVSFAQQPGARQAPPPAVVVEKIEVQQVSNPAQFTGRV
EAIEAVDIRARVTGFLHSVDFKDGQAVKAGDTLFEIEPDQLNALVASARAQVARADATRISAERSLARNRDLLARRTVSQ
ATVDEVQAAFDVASADVQVAQAALDTAELNLSYAHITAPISGSIGRATFTTGNLVGPDSGSLARIVSLDTVRVAFAVTEG
LLVTIRQQQASGGGLDPNTLKLSLRLANGTDYGTPGRIEFIENEIDPQTGTVAARAVFPNPRHILLPGQFVTLYVQEKDA
PSLPVVPQTAVLQDREGRFVYLLGKDNIVSQQRIHTGARVGNGWAVTKGLDGGEQVVVEGIQRLAQGMTVQPSQGQPVGG
GT
>Mature_402_residues
MRAPCERSERHRSVARRRWISRASWACLMAACLGGAAPPFVSSVSFAQQPGARQAPPPAVVVEKIEVQQVSNPAQFTGRV
EAIEAVDIRARVTGFLHSVDFKDGQAVKAGDTLFEIEPDQLNALVASARAQVARADATRISAERSLARNRDLLARRTVSQ
ATVDEVQAAFDVASADVQVAQAALDTAELNLSYAHITAPISGSIGRATFTTGNLVGPDSGSLARIVSLDTVRVAFAVTEG
LLVTIRQQQASGGGLDPNTLKLSLRLANGTDYGTPGRIEFIENEIDPQTGTVAARAVFPNPRHILLPGQFVTLYVQEKDA
PSLPVVPQTAVLQDREGRFVYLLGKDNIVSQQRIHTGARVGNGWAVTKGLDGGEQVVVEGIQRLAQGMTVQPSQGQPVGG
GT

Specific function: May contribute to resistance to some drugs, such as deoxycholate, sodium dodecyl sulfate and nalidixic acid, in the absence of BepD and BepE [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789929, Length=322, Percent_Identity=33.8509316770186, Blast_Score=177, Evalue=1e-45,
Organism=Escherichia coli, GI1786668, Length=346, Percent_Identity=33.2369942196532, Blast_Score=165, Evalue=6e-42,
Organism=Escherichia coli, GI1789665, Length=343, Percent_Identity=30.6122448979592, Blast_Score=157, Evalue=2e-39,
Organism=Escherichia coli, GI87082036, Length=326, Percent_Identity=30.9815950920245, Blast_Score=152, Evalue=5e-38,
Organism=Escherichia coli, GI87081796, Length=331, Percent_Identity=25.9818731117825, Blast_Score=75, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 42722; Mature: 42722

Theoretical pI: Translated: 8.09; Mature: 8.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRAPCERSERHRSVARRRWISRASWACLMAACLGGAAPPFVSSVSFAQQPGARQAPPPAV
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCE
VVEKIEVQQVSNPAQFTGRVEAIEAVDIRARVTGFLHSVDFKDGQAVKAGDTLFEIEPDQ
EEEHHHHHHCCCCHHHCCCEEEEEEEHHHHHHHHHHEECCCCCCCEEECCCEEEEECHHH
LNALVASARAQVARADATRISAERSLARNRDLLARRTVSQATVDEVQAAFDVASADVQVA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QAALDTAELNLSYAHITAPISGSIGRATFTTGNLVGPDSGSLARIVSLDTVRVAFAVTEG
HHHHHHHHEEEEEEEEEECCCCCCCCEEEECCCEECCCCCCEEEEEEHHHHEEEEEECCC
LLVTIRQQQASGGGLDPNTLKLSLRLANGTDYGTPGRIEFIENEIDPQTGTVAARAVFPN
EEEEEEECCCCCCCCCCCEEEEEEEEECCCCCCCCCEEEEECCCCCCCCCCEEEEEECCC
PRHILLPGQFVTLYVQEKDAPSLPVVPQTAVLQDREGRFVYLLGKDNIVSQQRIHTGARV
CCEEEECCCEEEEEEECCCCCCCCCCCCHHHEECCCCCEEEEECCCCCCHHHHHHCCCCC
GNGWAVTKGLDGGEQVVVEGIQRLAQGMTVQPSQGQPVGGGT
CCCEEEECCCCCHHHHHHHHHHHHHCCCEECCCCCCCCCCCC
>Mature Secondary Structure
MRAPCERSERHRSVARRRWISRASWACLMAACLGGAAPPFVSSVSFAQQPGARQAPPPAV
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCE
VVEKIEVQQVSNPAQFTGRVEAIEAVDIRARVTGFLHSVDFKDGQAVKAGDTLFEIEPDQ
EEEHHHHHHCCCCHHHCCCEEEEEEEHHHHHHHHHHEECCCCCCCEEECCCEEEEECHHH
LNALVASARAQVARADATRISAERSLARNRDLLARRTVSQATVDEVQAAFDVASADVQVA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QAALDTAELNLSYAHITAPISGSIGRATFTTGNLVGPDSGSLARIVSLDTVRVAFAVTEG
HHHHHHHHEEEEEEEEEECCCCCCCCEEEECCCEECCCCCCEEEEEEHHHHEEEEEECCC
LLVTIRQQQASGGGLDPNTLKLSLRLANGTDYGTPGRIEFIENEIDPQTGTVAARAVFPN
EEEEEEECCCCCCCCCCCEEEEEEEEECCCCCCCCCEEEEECCCCCCCCCCEEEEEECCC
PRHILLPGQFVTLYVQEKDAPSLPVVPQTAVLQDREGRFVYLLGKDNIVSQQRIHTGARV
CCEEEECCCEEEEEEECCCCCCCCCCCCHHHEECCCCCEEEEECCCCCCHHHHHHCCCCC
GNGWAVTKGLDGGEQVVVEGIQRLAQGMTVQPSQGQPVGGGT
CCCEEEECCCCCHHHHHHHHHHHHHCCCEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12271122 [H]