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The map label for this gene is pduE [H]

Identifier: 13475606

GI number: 13475606

Start: 5511505

End: 5511924

Strand: Reverse

Name: pduE [H]

Synonym: mll6721

Alternate gene names: 13475606

Gene position: 5511924-5511505 (Counterclockwise)

Preceding gene: 13475607

Following gene: 13475605

Centisome position: 78.34

GC content: 63.57

Gene sequence:

>420_bases
GTGACCCACACGCGCGCCGACTATCCGCTCGCCGAAACACAGCCCGGCGCCGTAAAGGGCAAGCGCGGCAAGTCGCTTGC
CGAGATAACGCTGGATTCGGTGCTTGCGGGCGACGTGACGATGGAAGACCTTCGTATCACCCCACAAGCGCTGCAGGCAC
AGGCCGATGTGGCGCGCGATGTCGGACGGCCGACACTGGCGCTCAACTTCGAGCGTGGGGCGGAGCTTGTCGAGGTGCCG
CAGGATTTCATCATGCAGGTATACGAACTGCTGCGCCCCGGCCGCGCCAAATCCAAGGAAGAATTGCTCCAGGCCGCCAC
CACGATGCGCGATACCTACCAGGCCGAACGCATCGCCCGTTTCATCGAAGAAGCCGCCGAGACCTACGCGGCTCGCGGCC
TTTTCACATTCCGCTTCTGA

Upstream 100 bases:

>100_bases
ACCACGCCCGCGTCGCCCTCATCTACGCCATCGAAACCGGACTGACGGAGGCTGGTGCGGCTCCCGAAGAGGTCGACGTC
ATCCTGACAGGAGTGAAATC

Downstream 100 bases:

>100_bases
AAGGACCGCATGAGCTGGAAAACCGGATTCCGTTCGCTCTACTATCTCGGTGCGCCCGAGCCGGATGATCCGGTGCTGGT
GCCGGCGCAAACCGCCATCC

Product: glycerol dehydratase small subunit

Products: NA

Alternate protein names: Propanediol utilization protein pduE [H]

Number of amino acids: Translated: 139; Mature: 138

Protein sequence:

>139_residues
MTHTRADYPLAETQPGAVKGKRGKSLAEITLDSVLAGDVTMEDLRITPQALQAQADVARDVGRPTLALNFERGAELVEVP
QDFIMQVYELLRPGRAKSKEELLQAATTMRDTYQAERIARFIEEAAETYAARGLFTFRF

Sequences:

>Translated_139_residues
MTHTRADYPLAETQPGAVKGKRGKSLAEITLDSVLAGDVTMEDLRITPQALQAQADVARDVGRPTLALNFERGAELVEVP
QDFIMQVYELLRPGRAKSKEELLQAATTMRDTYQAERIARFIEEAAETYAARGLFTFRF
>Mature_138_residues
THTRADYPLAETQPGAVKGKRGKSLAEITLDSVLAGDVTMEDLRITPQALQAQADVARDVGRPTLALNFERGAELVEVPQ
DFIMQVYELLRPGRAKSKEELLQAATTMRDTYQAERIARFIEEAAETYAARGLFTFRF

Specific function: Part of the pduCDE complex that catalyzes the dehydration of 1,2-propanediol to propionaldehyde. Is required for S.typhimurium growth on 1,2-propanediol as the sole carbon and energy source [H]

COG id: COG4910

COG function: function code Q; Propanediol dehydratase, small subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diol/glycerol dehydratase small subunit family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003207 [H]

Pfam domain/function: PF02287 Dehydratase_SU [H]

EC number: =4.2.1.28 [H]

Molecular weight: Translated: 15504; Mature: 15373

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHTRADYPLAETQPGAVKGKRGKSLAEITLDSVLAGDVTMEDLRITPQALQAQADVARD
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHCCHHHHHHHHHHHHH
VGRPTLALNFERGAELVEVPQDFIMQVYELLRPGRAKSKEELLQAATTMRDTYQAERIAR
CCCCEEEEEHHHCCHHHHCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
FIEEAAETYAARGLFTFRF
HHHHHHHHHHHHCEEEECC
>Mature Secondary Structure 
THTRADYPLAETQPGAVKGKRGKSLAEITLDSVLAGDVTMEDLRITPQALQAQADVARD
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHCCHHHHHHHHHHHHH
VGRPTLALNFERGAELVEVPQDFIMQVYELLRPGRAKSKEELLQAATTMRDTYQAERIAR
CCCCEEEEEHHHCCHHHHCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
FIEEAAETYAARGLFTFRF
HHHHHHHHHHHHCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9352910; 10498708; 11677609 [H]