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The map label for this gene is fixI [H]

Identifier: 13475529

GI number: 13475529

Start: 5424369

End: 5426657

Strand: Reverse

Name: fixI [H]

Synonym: mll6624

Alternate gene names: 13475529

Gene position: 5426657-5424369 (Counterclockwise)

Preceding gene: 13475530

Following gene: 13475528

Centisome position: 77.13

GC content: 62.47

Gene sequence:

>2289_bases
ATGAGCTGTTGTGCACCGGGCGCCGAAATGGCGCGGGATCTGGAAAGCAGCATGTCCGTCCTGCCATCAAGCCAGGAGAT
CAGGTTGGCGAGCCGATCGCTCGGCGGTGGTCTTAACCAAACTGACCTTTCAGTCCCAATGGTTCACTGCGCAGCCTGCA
TCCAGACGATCGAGACGGCACTGGGAAAGCTCGACAATGTCGAAGGCGCGCGTGTCAACCTGTCGACGAAACGGGTCGCG
ATCCGGTGGCGTGGCAATGAGGTACCACCCTTCGTCGCCGCGCTGGGACGGCTGGGCTATCAGGCGCACCTGTTCGAAGC
CGAAATTGATAACAAGGACAAGACGCTTGCCGAACTGATCCGCGCTGTCGCTGTTGCCGGCTTTGCAGCGGGCAACATCA
TGCTGCTTTCGGTCTCGGTCTGGTCCGGCGCCGAAGGTGCTACCCGCGACCTGTTCCATTGGGTCTCGGCGCTGATCGCC
ATCCCTGCACTTGCATTCTCCGGCGGAATCTTCTTCCGGTCGGCCTGGAACGCGTTGCGCCACGGCCGCATGAACATGGA
CGTACCGATCGCGGTCGGGGTGTCGCTTGCCTATGCCATGAGCCTCTATGAGACGATCAACCACGGCGACCATGCCTATT
TCGACGCGTCGGTTTCGTTGCTGTTCTTCCTGTTGATCGGCCGCACGCTGGATCACGTAATGCGGGAGCGTGCCCGGACC
GCGGTGAAAAGCCTGTCACAGTTGGCCGCGCGTGGCGCCATGGTGCTGCGCGGCGACGGCGCACGGGATTATCTGCCGGT
TGGCGAGATCGAACCCGGCATGCAACTGCTGATAGCAGCCGGTGAGAGAATCCCAGTCGACGGCAAGATCATTCAAGGGG
CGTCGGATCTCGACTGCTCACTGGTCTCCGGCGAGAGCACGCCCAGAAACGTGGCGCCGGGCGAGCCCGTGCAGGCTGGC
GTGCTCAACCTTACCGGCTCGCTGACGATCGAGGCGACAGCCGCGGCGAAGGATTCCTTCCTGGCCGAAATGGTCCGGCT
GATGGAGGCCGCGGAGGGCGGCCGCTCGCAGTATCGCCGCATCGCCGATCGCGTCTCGGCACTCTACGCGCCCGTGGTTC
ATCTCACCGCCTTCGTGACATTCCTGGGTTGGATGGCGGCGACCGGCGACTGGCACCGCGCGATGACGATCGCCATCGCT
GTTCTGATCATCACATGCCCGTGCGCGCTCGGTCTCGCAGTCCCGATCGTACAGGTGGTCGCCGCGCGGCGCCTGTTCGA
GAACGGCATTATGGTCAAGGACGGCTCGGCCATGGAACGCCTGGCAACAATCGATACGGCGGTGTTCGACAAGACCGGAA
CGCTCACGCTCGGCCAGCCCCGACTTGTCAACGCGTCATCGATCGATCCGGCCATGCTGTCAATCGCAGCCGACATGGCC
GCGCATTCGCGTCATCCATTTTCAAAGGCCATAGCCGGTTTTGCCGCTCCTGGCGGGCAACACAAATTCGATGCGGTCAC
CGAGCACCCGGGATTTGGAATCGAAGCCACTGACGGCGGAAGCACTTGGCGGCTGGGTCGACGGGGATGGGCTGGGTGGA
AGGCTCGAACCGGTGGTGAAGGCAAACATGGCTACGGCGGAACGGTCCTGACGAAAAACGGGATGATTGTCGCATGCTTT
GTTTTCGAGGATGCATTGCGTGCCGACGCCGGTGCAGCCATCGATCAATTGAAGCATGCAGGGATGTCGGTGGAAATGCT
GTCAGGCGATACCGCGGCAGCCTGCGCCGAGGTGGCAAAGGTGCTCGGTGTCGATGACTTCGTTCCCTGCCTGCTGCCAT
CCGGCAAAGTCGAACGGATCGAGACCCTGGCGGAAGACGGGCACAAGGTTCTGATGGTGGGCGACGGGCTCAACGACACG
CCGGCGCTGCGCGCGGCGCATGTCTCGATCGCACCGGCCACCGCCGCCGACATTGGCCGCAATGCGGCCGACTTTGTCTT
CCTGCGCGAAAGCCTGCTGGCTGTGCCGCTGGCACTTGACGTCTCGCGCAAGGCGGGACAACTGATCCGCCAGAACATCG
CGATCGCCATCGTTTACAATGCCGTCGCCGTGCCGGTCGCCATCCTGGGGCACGTGACGCCATTGATAGCGGCGATCGCC
ATGTCCGCCTCGTCGCTGCTTGTCATTGGAAATGCGCTGCGATTGCAAGCATCGATGCCAAATTCACCGGCGAAGGATGT
TCGCCGAAACAGGCAATCCGACTTCAACGAACTGGCCCGATCGTCATGA

Upstream 100 bases:

>100_bases
CGAAGGACGGCGTTTGGATCGTCGAGGTGGACGCCGATGCAGGTCTCGCGAAGCCCTATCGCGACGTCCGTAGGATCATG
ATTTCCCATGGAGCGCTGAA

Downstream 100 bases:

>100_bases
CAAACCTCACCTACCTCATACCCGCAGCCCTCTTCCTTGGGGCGCTTGGCCTTGGGGGTTTCCTGTGGGCTTTGAAAAGT
GGCCAGTATGAAGATCTAGA

Product: nitrogen fixation protein fixI

Products: ADP; Orthophosphate. [C]

Alternate protein names: E1-E2 type cation ATPase FixI [H]

Number of amino acids: Translated: 762; Mature: 761

Protein sequence:

>762_residues
MSCCAPGAEMARDLESSMSVLPSSQEIRLASRSLGGGLNQTDLSVPMVHCAACIQTIETALGKLDNVEGARVNLSTKRVA
IRWRGNEVPPFVAALGRLGYQAHLFEAEIDNKDKTLAELIRAVAVAGFAAGNIMLLSVSVWSGAEGATRDLFHWVSALIA
IPALAFSGGIFFRSAWNALRHGRMNMDVPIAVGVSLAYAMSLYETINHGDHAYFDASVSLLFFLLIGRTLDHVMRERART
AVKSLSQLAARGAMVLRGDGARDYLPVGEIEPGMQLLIAAGERIPVDGKIIQGASDLDCSLVSGESTPRNVAPGEPVQAG
VLNLTGSLTIEATAAAKDSFLAEMVRLMEAAEGGRSQYRRIADRVSALYAPVVHLTAFVTFLGWMAATGDWHRAMTIAIA
VLIITCPCALGLAVPIVQVVAARRLFENGIMVKDGSAMERLATIDTAVFDKTGTLTLGQPRLVNASSIDPAMLSIAADMA
AHSRHPFSKAIAGFAAPGGQHKFDAVTEHPGFGIEATDGGSTWRLGRRGWAGWKARTGGEGKHGYGGTVLTKNGMIVACF
VFEDALRADAGAAIDQLKHAGMSVEMLSGDTAAACAEVAKVLGVDDFVPCLLPSGKVERIETLAEDGHKVLMVGDGLNDT
PALRAAHVSIAPATAADIGRNAADFVFLRESLLAVPLALDVSRKAGQLIRQNIAIAIVYNAVAVPVAILGHVTPLIAAIA
MSASSLLVIGNALRLQASMPNSPAKDVRRNRQSDFNELARSS

Sequences:

>Translated_762_residues
MSCCAPGAEMARDLESSMSVLPSSQEIRLASRSLGGGLNQTDLSVPMVHCAACIQTIETALGKLDNVEGARVNLSTKRVA
IRWRGNEVPPFVAALGRLGYQAHLFEAEIDNKDKTLAELIRAVAVAGFAAGNIMLLSVSVWSGAEGATRDLFHWVSALIA
IPALAFSGGIFFRSAWNALRHGRMNMDVPIAVGVSLAYAMSLYETINHGDHAYFDASVSLLFFLLIGRTLDHVMRERART
AVKSLSQLAARGAMVLRGDGARDYLPVGEIEPGMQLLIAAGERIPVDGKIIQGASDLDCSLVSGESTPRNVAPGEPVQAG
VLNLTGSLTIEATAAAKDSFLAEMVRLMEAAEGGRSQYRRIADRVSALYAPVVHLTAFVTFLGWMAATGDWHRAMTIAIA
VLIITCPCALGLAVPIVQVVAARRLFENGIMVKDGSAMERLATIDTAVFDKTGTLTLGQPRLVNASSIDPAMLSIAADMA
AHSRHPFSKAIAGFAAPGGQHKFDAVTEHPGFGIEATDGGSTWRLGRRGWAGWKARTGGEGKHGYGGTVLTKNGMIVACF
VFEDALRADAGAAIDQLKHAGMSVEMLSGDTAAACAEVAKVLGVDDFVPCLLPSGKVERIETLAEDGHKVLMVGDGLNDT
PALRAAHVSIAPATAADIGRNAADFVFLRESLLAVPLALDVSRKAGQLIRQNIAIAIVYNAVAVPVAILGHVTPLIAAIA
MSASSLLVIGNALRLQASMPNSPAKDVRRNRQSDFNELARSS
>Mature_761_residues
SCCAPGAEMARDLESSMSVLPSSQEIRLASRSLGGGLNQTDLSVPMVHCAACIQTIETALGKLDNVEGARVNLSTKRVAI
RWRGNEVPPFVAALGRLGYQAHLFEAEIDNKDKTLAELIRAVAVAGFAAGNIMLLSVSVWSGAEGATRDLFHWVSALIAI
PALAFSGGIFFRSAWNALRHGRMNMDVPIAVGVSLAYAMSLYETINHGDHAYFDASVSLLFFLLIGRTLDHVMRERARTA
VKSLSQLAARGAMVLRGDGARDYLPVGEIEPGMQLLIAAGERIPVDGKIIQGASDLDCSLVSGESTPRNVAPGEPVQAGV
LNLTGSLTIEATAAAKDSFLAEMVRLMEAAEGGRSQYRRIADRVSALYAPVVHLTAFVTFLGWMAATGDWHRAMTIAIAV
LIITCPCALGLAVPIVQVVAARRLFENGIMVKDGSAMERLATIDTAVFDKTGTLTLGQPRLVNASSIDPAMLSIAADMAA
HSRHPFSKAIAGFAAPGGQHKFDAVTEHPGFGIEATDGGSTWRLGRRGWAGWKARTGGEGKHGYGGTVLTKNGMIVACFV
FEDALRADAGAAIDQLKHAGMSVEMLSGDTAAACAEVAKVLGVDDFVPCLLPSGKVERIETLAEDGHKVLMVGDGLNDTP
ALRAAHVSIAPATAADIGRNAADFVFLRESLLAVPLALDVSRKAGQLIRQNIAIAIVYNAVAVPVAILGHVTPLIAAIAM
SASSLLVIGNALRLQASMPNSPAKDVRRNRQSDFNELARSS

Specific function: FixI is a pump of a specific cation involved in symbiotic nitrogen fixation. The four proteins FixG, FixH, FixI, and FixS may participate in a membrane-bound complex coupling the FixI cation pump with a redox process catalyzed by FixG [H]

COG id: COG2217

COG function: function code P; Cation transport ATPase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HMA domain [H]

Homologues:

Organism=Homo sapiens, GI55743071, Length=783, Percent_Identity=27.0753512132823, Blast_Score=270, Evalue=5e-72,
Organism=Homo sapiens, GI55743073, Length=544, Percent_Identity=28.4926470588235, Blast_Score=199, Evalue=1e-50,
Organism=Homo sapiens, GI115529486, Length=608, Percent_Identity=26.1513157894737, Blast_Score=192, Evalue=1e-48,
Organism=Escherichia coli, GI1786691, Length=727, Percent_Identity=31.9119669876204, Blast_Score=328, Evalue=6e-91,
Organism=Escherichia coli, GI1789879, Length=706, Percent_Identity=29.1784702549575, Blast_Score=223, Evalue=4e-59,
Organism=Escherichia coli, GI1786914, Length=503, Percent_Identity=25.4473161033797, Blast_Score=120, Evalue=3e-28,
Organism=Escherichia coli, GI2367363, Length=289, Percent_Identity=25.2595155709343, Blast_Score=72, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17556548, Length=372, Percent_Identity=30.6451612903226, Blast_Score=171, Evalue=2e-42,
Organism=Saccharomyces cerevisiae, GI6320475, Length=650, Percent_Identity=27.3846153846154, Blast_Score=214, Evalue=4e-56,
Organism=Saccharomyces cerevisiae, GI6319772, Length=609, Percent_Identity=27.5862068965517, Blast_Score=201, Evalue=4e-52,
Organism=Saccharomyces cerevisiae, GI6321430, Length=550, Percent_Identity=25.2727272727273, Blast_Score=118, Evalue=4e-27,
Organism=Saccharomyces cerevisiae, GI6325221, Length=546, Percent_Identity=24.9084249084249, Blast_Score=112, Evalue=2e-25,
Organism=Drosophila melanogaster, GI221329854, Length=553, Percent_Identity=29.2947558770344, Blast_Score=191, Evalue=1e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008250
- InterPro:   IPR006403
- InterPro:   IPR000695
- InterPro:   IPR006416
- InterPro:   IPR001757
- InterPro:   IPR018303
- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR017969
- InterPro:   IPR006121
- InterPro:   IPR000150 [H]

Pfam domain/function: PF00122 E1-E2_ATPase; PF00702 Hydrolase [H]

EC number: 3.6.3.4 [C]

Molecular weight: Translated: 80361; Mature: 80229

Theoretical pI: Translated: 7.01; Mature: 7.01

Prosite motif: PS00154 ATPASE_E1_E2 ; PS01047 HMA_1 ; PS50846 HMA_2 ; PS00211 ABC_TRANSPORTER_1 ; PS01229 COF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSCCAPGAEMARDLESSMSVLPSSQEIRLASRSLGGGLNQTDLSVPMVHCAACIQTIETA
CCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHH
LGKLDNVEGARVNLSTKRVAIRWRGNEVPPFVAALGRLGYQAHLFEAEIDNKDKTLAELI
HHHCCCCCCCEEEEEEEEEEEEECCCCCCHHHHHHHHCCCEEEEEEECCCCCHHHHHHHH
RAVAVAGFAAGNIMLLSVSVWSGAEGATRDLFHWVSALIAIPALAFSGGIFFRSAWNALR
HHHHHHHHCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
HGRMNMDVPIAVGVSLAYAMSLYETINHGDHAYFDASVSLLFFLLIGRTLDHVMRERART
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHH
AVKSLSQLAARGAMVLRGDGARDYLPVGEIEPGMQLLIAAGERIPVDGKIIQGASDLDCS
HHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCHHHHEECCCCCCCCCEEECCCCCCCEE
LVSGESTPRNVAPGEPVQAGVLNLTGSLTIEATAAAKDSFLAEMVRLMEAAEGGRSQYRR
EECCCCCCCCCCCCCCCCCCCEEECCCEEEEEEHHHHHHHHHHHHHHHHHHCCCHHHHHH
IADRVSALYAPVVHLTAFVTFLGWMAATGDWHRAMTIAIAVLIITCPCALGLAVPIVQVV
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AARRLFENGIMVKDGSAMERLATIDTAVFDKTGTLTLGQPRLVNASSIDPAMLSIAADMA
HHHHHHHCCCEEECCHHHHHHHHHHHHHHCCCCCEECCCCCEECCCCCCHHHHHHHHHHH
AHSRHPFSKAIAGFAAPGGQHKFDAVTEHPGFGIEATDGGSTWRLGRRGWAGWKARTGGE
HHCCCCHHHHHHHHCCCCCCCCCCHHCCCCCCCEEECCCCCEEECCCCCCCCCCCCCCCC
GKHGYGGTVLTKNGMIVACFVFEDALRADAGAAIDQLKHAGMSVEMLSGDTAAACAEVAK
CCCCCCCEEEECCCCEEEEEEEHHHHHHCCHHHHHHHHHCCCEEEEECCCHHHHHHHHHH
VLGVDDFVPCLLPSGKVERIETLAEDGHKVLMVGDGLNDTPALRAAHVSIAPATAADIGR
HHCCCHHHHHHCCCCHHHHHHHHHHCCCEEEEEECCCCCCCCHHEEEEEECCCHHHHHCC
NAADFVFLRESLLAVPLALDVSRKAGQLIRQNIAIAIVYNAVAVPVAILGHVTPLIAAIA
CHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCEEEEEHHHHHHHHHHHHHHHHHHHHHH
MSASSLLVIGNALRLQASMPNSPAKDVRRNRQSDFNELARSS
HCCCCEEEECCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SCCAPGAEMARDLESSMSVLPSSQEIRLASRSLGGGLNQTDLSVPMVHCAACIQTIETA
CCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHH
LGKLDNVEGARVNLSTKRVAIRWRGNEVPPFVAALGRLGYQAHLFEAEIDNKDKTLAELI
HHHCCCCCCCEEEEEEEEEEEEECCCCCCHHHHHHHHCCCEEEEEEECCCCCHHHHHHHH
RAVAVAGFAAGNIMLLSVSVWSGAEGATRDLFHWVSALIAIPALAFSGGIFFRSAWNALR
HHHHHHHHCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
HGRMNMDVPIAVGVSLAYAMSLYETINHGDHAYFDASVSLLFFLLIGRTLDHVMRERART
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHH
AVKSLSQLAARGAMVLRGDGARDYLPVGEIEPGMQLLIAAGERIPVDGKIIQGASDLDCS
HHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCHHHHEECCCCCCCCCEEECCCCCCCEE
LVSGESTPRNVAPGEPVQAGVLNLTGSLTIEATAAAKDSFLAEMVRLMEAAEGGRSQYRR
EECCCCCCCCCCCCCCCCCCCEEECCCEEEEEEHHHHHHHHHHHHHHHHHHCCCHHHHHH
IADRVSALYAPVVHLTAFVTFLGWMAATGDWHRAMTIAIAVLIITCPCALGLAVPIVQVV
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AARRLFENGIMVKDGSAMERLATIDTAVFDKTGTLTLGQPRLVNASSIDPAMLSIAADMA
HHHHHHHCCCEEECCHHHHHHHHHHHHHHCCCCCEECCCCCEECCCCCCHHHHHHHHHHH
AHSRHPFSKAIAGFAAPGGQHKFDAVTEHPGFGIEATDGGSTWRLGRRGWAGWKARTGGE
HHCCCCHHHHHHHHCCCCCCCCCCHHCCCCCCCEEECCCCCEEECCCCCCCCCCCCCCCC
GKHGYGGTVLTKNGMIVACFVFEDALRADAGAAIDQLKHAGMSVEMLSGDTAAACAEVAK
CCCCCCCEEEECCCCEEEEEEEHHHHHHCCHHHHHHHHHCCCEEEEECCCHHHHHHHHHH
VLGVDDFVPCLLPSGKVERIETLAEDGHKVLMVGDGLNDTPALRAAHVSIAPATAADIGR
HHCCCHHHHHHCCCCHHHHHHHHHHCCCEEEEEECCCCCCCCHHEEEEEECCCHHHHHCC
NAADFVFLRESLLAVPLALDVSRKAGQLIRQNIAIAIVYNAVAVPVAILGHVTPLIAAIA
CHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCEEEEEHHHHHHHHHHHHHHHHHHHHHH
MSASSLLVIGNALRLQASMPNSPAKDVRRNRQSDFNELARSS
HCCCCEEEECCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Cu [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; H2O [C]

Specific reaction: ATP + H2O = ADP + Orthophosphate. [C]

General reaction: Phosphorous acid anhydride hydrolysis [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2536685; 11481432 [H]