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The map label for this gene is potF [H]

Identifier: 13475456

GI number: 13475456

Start: 5337527

End: 5338624

Strand: Direct

Name: potF [H]

Synonym: mlr6533

Alternate gene names: 13475456

Gene position: 5337527-5338624 (Clockwise)

Preceding gene: 13475455

Following gene: 13475457

Centisome position: 75.86

GC content: 57.47

Gene sequence:

>1098_bases
ATGATCCGCAAAGCGCTCTGGCTTTCGGCTACCTCGGTATTTCTGACATTGTTCACACCTGCCGGCCACGCCGAGGACCG
TGTTGTCAACGTCTTCAACTGGTCGGATTACATCGACAGCTCCGTCATCGAGGGCTTCACCAAGCAGACCGGCATCAAGG
TCGTCTACGACACCTTCGATTCCAACGAGATCCTGGAAACGAAGCTGCTTGCCGGTGGTAGCGGCTACGACGTCGTCGTA
CCCAGCGGCAATTTCCTTGCCCGCCAGATCCAGGCCGGCGTCTTCCAGAAGCTCGACAAGTCGAAGCTACCGAACATTTC
CAACATGTGGGACGTCATTTCGGAACGCACCGCCAAGTACGATCCCGGTAACGAATATTCGGTCAACTACATGTGGGGCA
CCGTCGGCATCGGCTATAACGTCAAGAAGGTGAAAGCGGCGCTCGGCACCGACAAGATCGACAGTTGGGATGTGTTCTTC
AACCCCGAAAGTCTCGCCAAGCTGAAGGATTGCGGCGTCTATGTGCTGGATTCGCCGGCCGACATCATTCCGGCGGCGCT
GAAATATGTCGGCCTCGACCCGAACAGCACCTCGCCTGACGATATCGCCAAGGCCGAGGAAGCCCTGATGAAGGTCCGCC
CCTTTATCAGGAAATTCCACTCGTCCGAATATATCAACGCCTTGGCCAACGGTGACATCTGCCTGGCTATCGGCTTCTCC
GGGGACGTCTTCCAGGCCCGCAACCGCGCCCAGGAGGCCAAGCAGGGTGTCGAGATCGGCTATTCCGTGCCGAAGGAAGG
CGCCCAGATATGGTTCGACCAGATGGCGATCCCCGCTGATGCGCCGCATGTCGCCGAGGCGCATGAATTCATCAACTACA
TGATGAAGCCGGAAGTCATCGCTAAGTCTTCAAACTTCGTGCTCTACGCCAACGGCAACAAGGCTTCGCAGCAGTTCGTC
GACAAGGCGATCTTGGACGATCCGTCAATCTATCCGGACGAAGCGACGGTGCAGAAGCTCTATACGGTCCAGCCGTACGA
TCCCAAGGCCCAGCGCGTCATCACGCGCACCTGGACCAAGATCGTCACCGGTCAATAA

Upstream 100 bases:

>100_bases
GCTTGTTGTCCCCTGGTAAGCTCAATAGTCTGAAACAAATGCCCGGTCGCGCGTATGTCGCGACGAGCCGGCGATGGAAC
ACTGACCAAAGGAGAATAGC

Downstream 100 bases:

>100_bases
GCATCTGAAGCGACCTCGGCGGACAGCTGCCGGGGTCGCGTTTTTGGGGCACAAAGCAAGAAACAGCACGGAGTGGGGAT
ATGAAATCGCTTGGCAGCAT

Product: ABC transporter, polyamine transport protein, periplasmic protein

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 365; Mature: 365

Protein sequence:

>365_residues
MIRKALWLSATSVFLTLFTPAGHAEDRVVNVFNWSDYIDSSVIEGFTKQTGIKVVYDTFDSNEILETKLLAGGSGYDVVV
PSGNFLARQIQAGVFQKLDKSKLPNISNMWDVISERTAKYDPGNEYSVNYMWGTVGIGYNVKKVKAALGTDKIDSWDVFF
NPESLAKLKDCGVYVLDSPADIIPAALKYVGLDPNSTSPDDIAKAEEALMKVRPFIRKFHSSEYINALANGDICLAIGFS
GDVFQARNRAQEAKQGVEIGYSVPKEGAQIWFDQMAIPADAPHVAEAHEFINYMMKPEVIAKSSNFVLYANGNKASQQFV
DKAILDDPSIYPDEATVQKLYTVQPYDPKAQRVITRTWTKIVTGQ

Sequences:

>Translated_365_residues
MIRKALWLSATSVFLTLFTPAGHAEDRVVNVFNWSDYIDSSVIEGFTKQTGIKVVYDTFDSNEILETKLLAGGSGYDVVV
PSGNFLARQIQAGVFQKLDKSKLPNISNMWDVISERTAKYDPGNEYSVNYMWGTVGIGYNVKKVKAALGTDKIDSWDVFF
NPESLAKLKDCGVYVLDSPADIIPAALKYVGLDPNSTSPDDIAKAEEALMKVRPFIRKFHSSEYINALANGDICLAIGFS
GDVFQARNRAQEAKQGVEIGYSVPKEGAQIWFDQMAIPADAPHVAEAHEFINYMMKPEVIAKSSNFVLYANGNKASQQFV
DKAILDDPSIYPDEATVQKLYTVQPYDPKAQRVITRTWTKIVTGQ
>Mature_365_residues
MIRKALWLSATSVFLTLFTPAGHAEDRVVNVFNWSDYIDSSVIEGFTKQTGIKVVYDTFDSNEILETKLLAGGSGYDVVV
PSGNFLARQIQAGVFQKLDKSKLPNISNMWDVISERTAKYDPGNEYSVNYMWGTVGIGYNVKKVKAALGTDKIDSWDVFF
NPESLAKLKDCGVYVLDSPADIIPAALKYVGLDPNSTSPDDIAKAEEALMKVRPFIRKFHSSEYINALANGDICLAIGFS
GDVFQARNRAQEAKQGVEIGYSVPKEGAQIWFDQMAIPADAPHVAEAHEFINYMMKPEVIAKSSNFVLYANGNKASQQFV
DKAILDDPSIYPDEATVQKLYTVQPYDPKAQRVITRTWTKIVTGQ

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine. Polyamine binding protein [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787078, Length=363, Percent_Identity=51.7906336088154, Blast_Score=387, Evalue=1e-109,
Organism=Escherichia coli, GI1787367, Length=321, Percent_Identity=33.0218068535826, Blast_Score=181, Evalue=4e-47,
Organism=Escherichia coli, GI1787711, Length=293, Percent_Identity=24.9146757679181, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 40456; Mature: 40456

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRKALWLSATSVFLTLFTPAGHAEDRVVNVFNWSDYIDSSVIEGFTKQTGIKVVYDTFD
CCCHHHHHHHHEEEEEEECCCCCCCCCEEEEECCHHHHHHHHHHHHHHCCCCEEEEECCC
SNEILETKLLAGGSGYDVVVPSGNFLARQIQAGVFQKLDKSKLPNISNMWDVISERTAKY
CCCHHHHHEEECCCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCC
DPGNEYSVNYMWGTVGIGYNVKKVKAALGTDKIDSWDVFFNPESLAKLKDCGVYVLDSPA
CCCCCEEEEEEEEEEECCCCHHHHHHHHCCCCCCCEEEEECHHHHHHHHHCCEEEECCCH
DIIPAALKYVGLDPNSTSPDDIAKAEEALMKVRPFIRKFHSSEYINALANGDICLAIGFS
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCEEEEEECC
GDVFQARNRAQEAKQGVEIGYSVPKEGAQIWFDQMAIPADAPHVAEAHEFINYMMKPEVI
CCHHHHHHHHHHHHCCCEECCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCHHE
AKSSNFVLYANGNKASQQFVDKAILDDPSIYPDEATVQKLYTVQPYDPKAQRVITRTWTK
ECCCCEEEEECCCHHHHHHHHHHHCCCCCCCCCHHHHHHEEEECCCCCHHHHHHHHHHHH
IVTGQ
HHCCC
>Mature Secondary Structure
MIRKALWLSATSVFLTLFTPAGHAEDRVVNVFNWSDYIDSSVIEGFTKQTGIKVVYDTFD
CCCHHHHHHHHEEEEEEECCCCCCCCCEEEEECCHHHHHHHHHHHHHHCCCCEEEEECCC
SNEILETKLLAGGSGYDVVVPSGNFLARQIQAGVFQKLDKSKLPNISNMWDVISERTAKY
CCCHHHHHEEECCCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCC
DPGNEYSVNYMWGTVGIGYNVKKVKAALGTDKIDSWDVFFNPESLAKLKDCGVYVLDSPA
CCCCCEEEEEEEEEEECCCCHHHHHHHHCCCCCCCEEEEECHHHHHHHHHCCEEEECCCH
DIIPAALKYVGLDPNSTSPDDIAKAEEALMKVRPFIRKFHSSEYINALANGDICLAIGFS
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCEEEEEECC
GDVFQARNRAQEAKQGVEIGYSVPKEGAQIWFDQMAIPADAPHVAEAHEFINYMMKPEVI
CCHHHHHHHHHHHHCCCEECCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCHHE
AKSSNFVLYANGNKASQQFVDKAILDDPSIYPDEATVQKLYTVQPYDPKAQRVITRTWTK
ECCCCEEEEECCCHHHHHHHHHHHCCCCCCCCCHHHHHHEEEECCCCCHHHHHHHHHHHH
IVTGQ
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: Polyamine Binding Protein [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503; 9298646; 9651355 [H]