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The map label for this gene is folD1

Identifier: 13475440

GI number: 13475440

Start: 5318137

End: 5319036

Strand: Direct

Name: folD1

Synonym: mlr6508

Alternate gene names: 13475440

Gene position: 5318137-5319036 (Clockwise)

Preceding gene: 13475439

Following gene: 13475441

Centisome position: 75.58

GC content: 65.0

Gene sequence:

>900_bases
ATGGCCGAAGTGATTGACGGAAAGAGCGTTGCCGAGGATGTGGTGCGAACGGTCAAGGCGTTGACCGCCGAGCTGGTCGC
CAAGGGCAAAGCCAAGCCTGGTCTGGCCGTGGTCATCGTCGGCGAGGATCCGGCGAGCCAGGTCTATGTCGCTTCCAAGT
CGCGCACCGCCAAGGAATGCGGCTTTCATTCGCTCCAGCACACTCTGCCGGCCGAGACCTCCGAGGAGGCGCTGCTCAAG
ATCATCGCCGATCTCAACGCCGATCCGGCCGTCAACGGCATCCTGGTGCAGCTGCCGCTGCCCGCCCACATCGACGCCGG
CAAGATCATCCAGGCGATCGCGCCTCAGAAGGATGTCGACGGCTTCCATTTCATCAATGTCGGCAAGCTCGGCACAGGCG
AGCTGGATACGGCCTTTGTTCCTTGCACGCCGGCCGGCTCGATGCTTCTGATCCAGCGCGTGCGCGGCAAAGACCTGTCC
GGCCTCAACGCCGTCGTTGTCGGCCGCTCCAACATCGTCGGCAAGCCGATGGCCAATCTGCTGCTTGCCGCCAACTGCAC
CGTCACCATTGCCCACAGCCGCACCAAGGATCTGCCGGCGCTCGCCCGCACGGCCGACATCCTGGTCGCCGCCGTCGGCC
GGCCGGAAATGATCAAGGGGGATTGGGTTAAGCCGGGCGCCACCGTCATCGATGTCGGCATCAACCGCATTCCCGCACCC
GAGAAGGGCGAGGGCAAGTCGCGCCTCGTCGGCGATGTCGCCTATGCCGAGGCGGCCAGGCAGGCCGGCGCCATCACGCC
GGTGCCCGGTGGTGTCGGGCCGATGACCATTGCCATGCTGATGGCCAATACGTTAGCTTCTGCCTACCTTGCGGCCGGAT
TGAAGCGGCCCACCTTCTGA

Upstream 100 bases:

>100_bases
CGTGCCGGAATTTTCGTGACAATGCCGCGCTGCATGTTGCCGGCGCCGGGACATTGCGTCTCGAAAGCGGCGTTGCGCGA
AACAGGGTTGAGGATGGTTC

Downstream 100 bases:

>100_bases
GCCCGGGTTGACCCTGAAAGCGCCAGTTTTGCCGAACCCCACCATTTCCCCGGAACGGCCGGTCACGACTGATCCCGTTC
AAGGTGGCCGGCAGTTCGCC

Product: bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase

Number of amino acids: Translated: 299; Mature: 298

Protein sequence:

>299_residues
MAEVIDGKSVAEDVVRTVKALTAELVAKGKAKPGLAVVIVGEDPASQVYVASKSRTAKECGFHSLQHTLPAETSEEALLK
IIADLNADPAVNGILVQLPLPAHIDAGKIIQAIAPQKDVDGFHFINVGKLGTGELDTAFVPCTPAGSMLLIQRVRGKDLS
GLNAVVVGRSNIVGKPMANLLLAANCTVTIAHSRTKDLPALARTADILVAAVGRPEMIKGDWVKPGATVIDVGINRIPAP
EKGEGKSRLVGDVAYAEAARQAGAITPVPGGVGPMTIAMLMANTLASAYLAAGLKRPTF

Sequences:

>Translated_299_residues
MAEVIDGKSVAEDVVRTVKALTAELVAKGKAKPGLAVVIVGEDPASQVYVASKSRTAKECGFHSLQHTLPAETSEEALLK
IIADLNADPAVNGILVQLPLPAHIDAGKIIQAIAPQKDVDGFHFINVGKLGTGELDTAFVPCTPAGSMLLIQRVRGKDLS
GLNAVVVGRSNIVGKPMANLLLAANCTVTIAHSRTKDLPALARTADILVAAVGRPEMIKGDWVKPGATVIDVGINRIPAP
EKGEGKSRLVGDVAYAEAARQAGAITPVPGGVGPMTIAMLMANTLASAYLAAGLKRPTF
>Mature_298_residues
AEVIDGKSVAEDVVRTVKALTAELVAKGKAKPGLAVVIVGEDPASQVYVASKSRTAKECGFHSLQHTLPAETSEEALLKI
IADLNADPAVNGILVQLPLPAHIDAGKIIQAIAPQKDVDGFHFINVGKLGTGELDTAFVPCTPAGSMLLIQRVRGKDLSG
LNAVVVGRSNIVGKPMANLLLAANCTVTIAHSRTKDLPALARTADILVAAVGRPEMIKGDWVKPGATVIDVGINRIPAPE
KGEGKSRLVGDVAYAEAARQAGAITPVPGGVGPMTIAMLMANTLASAYLAAGLKRPTF

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family

Homologues:

Organism=Homo sapiens, GI222136639, Length=290, Percent_Identity=47.5862068965517, Blast_Score=254, Evalue=8e-68,
Organism=Homo sapiens, GI222418558, Length=297, Percent_Identity=43.7710437710438, Blast_Score=220, Evalue=1e-57,
Organism=Homo sapiens, GI94721354, Length=297, Percent_Identity=46.1279461279461, Blast_Score=220, Evalue=1e-57,
Organism=Homo sapiens, GI36796743, Length=224, Percent_Identity=28.5714285714286, Blast_Score=88, Evalue=8e-18,
Organism=Escherichia coli, GI1786741, Length=287, Percent_Identity=51.9163763066202, Blast_Score=273, Evalue=8e-75,
Organism=Caenorhabditis elegans, GI17568735, Length=297, Percent_Identity=42.7609427609428, Blast_Score=206, Evalue=2e-53,
Organism=Saccharomyces cerevisiae, GI6319558, Length=257, Percent_Identity=47.8599221789883, Blast_Score=242, Evalue=6e-65,
Organism=Saccharomyces cerevisiae, GI6321643, Length=298, Percent_Identity=42.2818791946309, Blast_Score=224, Evalue=9e-60,
Organism=Saccharomyces cerevisiae, GI6322933, Length=281, Percent_Identity=24.1992882562278, Blast_Score=72, Evalue=9e-14,
Organism=Drosophila melanogaster, GI62472483, Length=293, Percent_Identity=49.4880546075085, Blast_Score=257, Evalue=8e-69,
Organism=Drosophila melanogaster, GI45551871, Length=293, Percent_Identity=49.4880546075085, Blast_Score=257, Evalue=8e-69,
Organism=Drosophila melanogaster, GI24645718, Length=293, Percent_Identity=49.4880546075085, Blast_Score=257, Evalue=8e-69,
Organism=Drosophila melanogaster, GI17137370, Length=293, Percent_Identity=49.4880546075085, Blast_Score=257, Evalue=8e-69,
Organism=Drosophila melanogaster, GI17136816, Length=299, Percent_Identity=50.5016722408027, Blast_Score=253, Evalue=1e-67,
Organism=Drosophila melanogaster, GI17136818, Length=299, Percent_Identity=50.5016722408027, Blast_Score=252, Evalue=2e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FOLD1_RHILO (Q989A7)

Other databases:

- EMBL:   BA000012
- RefSeq:   NP_107004.1
- HSSP:   P11586
- ProteinModelPortal:   Q989A7
- SMR:   Q989A7
- GeneID:   1229659
- GenomeReviews:   BA000012_GR
- KEGG:   mlo:mlr6508
- NMPDR:   fig|266835.1.peg.5105
- HOGENOM:   HBG328751
- OMA:   TYVRNKQ
- ProtClustDB:   PRK14188
- BRENDA:   1.5.1.5
- BRENDA:   3.5.4.9
- GO:   GO:0005488
- HAMAP:   MF_01576
- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631
- Gene3D:   G3DSA:3.40.50.720
- PRINTS:   PR00085

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C

EC number: =1.5.1.5; =3.5.4.9

Molecular weight: Translated: 30891; Mature: 30760

Theoretical pI: Translated: 8.83; Mature: 8.83

Prosite motif: PS00766 THF_DHG_CYH_1; PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEVIDGKSVAEDVVRTVKALTAELVAKGKAKPGLAVVIVGEDPASQVYVASKSRTAKEC
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCEEEEECCCCCHHHH
GFHSLQHTLPAETSEEALLKIIADLNADPAVNGILVQLPLPAHIDAGKIIQAIAPQKDVD
HHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCHHHHHHHHCCCCCCC
GFHFINVGKLGTGELDTAFVPCTPAGSMLLIQRVRGKDLSGLNAVVVGRSNIVGKPMANL
CEEEEEECCCCCCCCCCEEECCCCCCHHHHHHHHCCCCCCCCEEEEEECCCCCCCHHHHH
LLAANCTVTIAHSRTKDLPALARTADILVAAVGRPEMIKGDWVKPGATVIDVGINRIPAP
EEECCCEEEEECCCCCCCHHHHHHHHHHEEECCCCCEECCCCCCCCCEEEEECCCCCCCC
EKGEGKSRLVGDVAYAEAARQAGAITPVPGGVGPMTIAMLMANTLASAYLAAGLKRPTF
CCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
AEVIDGKSVAEDVVRTVKALTAELVAKGKAKPGLAVVIVGEDPASQVYVASKSRTAKEC
CCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCEEEEECCCCCHHHH
GFHSLQHTLPAETSEEALLKIIADLNADPAVNGILVQLPLPAHIDAGKIIQAIAPQKDVD
HHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCHHHHHHHHCCCCCCC
GFHFINVGKLGTGELDTAFVPCTPAGSMLLIQRVRGKDLSGLNAVVVGRSNIVGKPMANL
CEEEEEECCCCCCCCCCEEECCCCCCHHHHHHHHCCCCCCCCEEEEEECCCCCCCHHHHH
LLAANCTVTIAHSRTKDLPALARTADILVAAVGRPEMIKGDWVKPGATVIDVGINRIPAP
EEECCCEEEEECCCCCCCHHHHHHHHHHEEECCCCCEECCCCCCCCCEEEEECCCCCCCC
EKGEGKSRLVGDVAYAEAARQAGAITPVPGGVGPMTIAMLMANTLASAYLAAGLKRPTF
CCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11214968