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The map label for this gene is fixI [H]

Identifier: 13475370

GI number: 13475370

Start: 5239036

End: 5241324

Strand: Direct

Name: fixI [H]

Synonym: mlr6417

Alternate gene names: 13475370

Gene position: 5239036-5241324 (Clockwise)

Preceding gene: 13475369

Following gene: 13475371

Centisome position: 74.46

GC content: 65.22

Gene sequence:

>2289_bases
ATGAGCTGTTGTGCACCCAGCGCGGAACTGGCGCTGGATCTGACCAGTACCACATCGGTCCTGCCGTCTAGCCAGGAGAT
CAGGTTGGCGAGCCGATCGCTTGGCGACAATCTTTGGCAGACCGACCTTTCAGTGCCGACGGTTCACTGCGCGGCGTGCA
TCCAGGCCATCGAGACGGCTCTCGGAAGGCTCGATCGCGTCGAAGGCGCTCGTGTCAACCTGTCGACGAAACGGGTCGCG
ATCCGGTGGCGTGGGGACTCTGTGCCTCCGTTTGTCGCCGCGCTGGGACGGCTGGGCTTTCAGGCGCACCTGTTCGAAGC
CGACCCTGATAAGAAGGACAAGACGCTTGCCGAGCTGATCCGCGCGGTCGCGGTCGCCGGGTTCGCCGCCGGCAACATCA
TGCTTCTTTCGGTCTCGGTCTGGTCCGGCGCCGAAGGTGCTACCCGCGACCTGTTTCACTGGGTCTCTGCATTGATCGCC
ATCCCGGCGCTCACCTTTGCCGGCGGCATCTTCTTCCGCTCGGCCTGGAATGCACTGCGCCACGGCCGCATGAACATGGA
CGTGCCGATCGCGGTTGGGGTGTCGCTCGCTTATGCCATGAGCCTCTACGAGACGATCAATCATGGCGATCACGCCTATT
TCGACGCGTCCGTATCCCTGCTGTTTTTCCTGTTGATCGGCCGCACCCTGGATCACGTCATGCGCGAACGGGCACGGACC
GCCGTGAAAGGCCTGTCCCAGTTGGCCGCACGTGGCGCCATGGTGCTGCGCGGCGATGGCGCGCGCGACTACCTGCCGGT
TGGCGAGATCGAACCGGGCATGCAACTGCTGGTCGCCGCCGGCGAAAGGGTGCCTGTCGACAGCAGGGTCATTCGCGGCG
CGTCGGATCTCGACTGCTCGCTGGTTTCCGGCGAGAGCACACCGAAAACCGTGGCGTCCGGGGAACAGGTTCAGGCCGGC
ACGCTCAATCTTACCGGCCCGCTGACCATCGAAGCGACCGCTGCCGCAAAGGACTCCTTCCTGGCGGAAATGGTTCGGCT
GATGGAAGCCCCCGAAGGTGGTCGTGCGCACTATCGGCGGATCGCCGACCGTGTCTCGGCGCTCTACGCGCCGGTGGTCC
ATCTCACCGCCTTCGTGACGTTCCTTGGCTGGATGGCGGTAACCGGCGACTGGCACCGGGCAGTGACGATCGCGATCGCC
GTTCTCATCATCACATGCCCGTGCGCGCTCGGTCTGGCCGTGCCGATCGTGCAAGTGGTCGCGGCGCGCCGCTTGTTCGA
AGCCGGCGTGATGGTCAAGGACGGATCGGCCATTGAGCGCCTGGCAGCGATAGACGTGGCGGTATTCGACAAGACCGGCA
CGCTAACGCTCGGTCAGCCCAGGCTGGTCAACGCGTCGACCATCGATCCGGCGATGCTGGCGATCGCAGCCGACATGGCG
GCGCACTCCCGTCACCCGTTCTCGAAGGCCATCGCCGCATTCGCGGGTTTTGCCGGCCAGCCGAAGCTGGAAGCCGTCAG
CGAGCACCCGGGTCTCGGCATCGAAGCGACGACCGCGGACAGCACCTGGCGTCTTGGCCGGCGCGGCTGGGCCGGATGGA
AAGCCCGGACCGGTGGCGAAGGCAAGTATGGCGGCTATGGCGGGACGGTGCTTTCGAAGAATGGGAGGATCGTCGCCTCC
TTCACGTTCGAGGACGCTACGCGCGCCGACGCCAAGGCGGCGGTCGGGCAATTGAAGGATGCCGGCGTGTCGGTCGAAAT
GCTGTCCGGGGATATAGTCCATGCCTGCGGCGAGGTTGCCGGGACGTTGGATATCGAGAGTTTCGTTCCGGCGCTGCTTC
CCTCCGGCAAGGTTGAGCGGATCGAGGTGCTGGCCAGGGCGGGCCACAAGGTTCTGATGGTGGGCGACGGGCTCAACGAC
ACGCCGGCCCTCGCTGCCGCGCATGTCTCGATCGCGCCGGCAACCGCCGCCGATATTGGCCGCAACGCGGCCGATTTCGT
GTTCCTGCGCGAAAGCCTGCTGGCGGTGCCCCTGGCGCTGGATGTCTCGCGGCAAGCGGGAAGGCTGATCCGGCAGAATA
TCGCCATTGCGATCGTCTACAACGCTGTCGCCGTGCCGATTGCCATTCTCGGGCACGTCACGCCGTTGATCGCGGCGATC
GCGATGTCGGCCTCATCGCTGCTGGTCATCGGAAACGGGCTGCGGCTGCAGGGCTTTAGGCTCGCCGAGCTGACGAGGGC
TTCTTCGGCAACGCATTCCGGCATTCACCCATCAGCGCGGTCATCATGA

Upstream 100 bases:

>100_bases
CAAGGGATGGCGTCTGGATCGTCGAAGTCGACGCCGATGCCGGTCTGACAGAGCCCTATCGCGACGTTCGCCGGATCATG
ATTTCGCAAGGAGCGCTGCA

Downstream 100 bases:

>100_bases
CGATACTCGTCTACCTCATGCCAGTCGCCCTATTTCTTGGCGCACTGGGGCTGACTGGCTTTCTGTGGGCGTTGAAGAGT
GGCCAATATGAGGATCTTGA

Product: nitrogen fixation protein fixI

Products: ADP; Orthophosphate. [C]

Alternate protein names: E1-E2 type cation ATPase FixI [H]

Number of amino acids: Translated: 762; Mature: 761

Protein sequence:

>762_residues
MSCCAPSAELALDLTSTTSVLPSSQEIRLASRSLGDNLWQTDLSVPTVHCAACIQAIETALGRLDRVEGARVNLSTKRVA
IRWRGDSVPPFVAALGRLGFQAHLFEADPDKKDKTLAELIRAVAVAGFAAGNIMLLSVSVWSGAEGATRDLFHWVSALIA
IPALTFAGGIFFRSAWNALRHGRMNMDVPIAVGVSLAYAMSLYETINHGDHAYFDASVSLLFFLLIGRTLDHVMRERART
AVKGLSQLAARGAMVLRGDGARDYLPVGEIEPGMQLLVAAGERVPVDSRVIRGASDLDCSLVSGESTPKTVASGEQVQAG
TLNLTGPLTIEATAAAKDSFLAEMVRLMEAPEGGRAHYRRIADRVSALYAPVVHLTAFVTFLGWMAVTGDWHRAVTIAIA
VLIITCPCALGLAVPIVQVVAARRLFEAGVMVKDGSAIERLAAIDVAVFDKTGTLTLGQPRLVNASTIDPAMLAIAADMA
AHSRHPFSKAIAAFAGFAGQPKLEAVSEHPGLGIEATTADSTWRLGRRGWAGWKARTGGEGKYGGYGGTVLSKNGRIVAS
FTFEDATRADAKAAVGQLKDAGVSVEMLSGDIVHACGEVAGTLDIESFVPALLPSGKVERIEVLARAGHKVLMVGDGLND
TPALAAAHVSIAPATAADIGRNAADFVFLRESLLAVPLALDVSRQAGRLIRQNIAIAIVYNAVAVPIAILGHVTPLIAAI
AMSASSLLVIGNGLRLQGFRLAELTRASSATHSGIHPSARSS

Sequences:

>Translated_762_residues
MSCCAPSAELALDLTSTTSVLPSSQEIRLASRSLGDNLWQTDLSVPTVHCAACIQAIETALGRLDRVEGARVNLSTKRVA
IRWRGDSVPPFVAALGRLGFQAHLFEADPDKKDKTLAELIRAVAVAGFAAGNIMLLSVSVWSGAEGATRDLFHWVSALIA
IPALTFAGGIFFRSAWNALRHGRMNMDVPIAVGVSLAYAMSLYETINHGDHAYFDASVSLLFFLLIGRTLDHVMRERART
AVKGLSQLAARGAMVLRGDGARDYLPVGEIEPGMQLLVAAGERVPVDSRVIRGASDLDCSLVSGESTPKTVASGEQVQAG
TLNLTGPLTIEATAAAKDSFLAEMVRLMEAPEGGRAHYRRIADRVSALYAPVVHLTAFVTFLGWMAVTGDWHRAVTIAIA
VLIITCPCALGLAVPIVQVVAARRLFEAGVMVKDGSAIERLAAIDVAVFDKTGTLTLGQPRLVNASTIDPAMLAIAADMA
AHSRHPFSKAIAAFAGFAGQPKLEAVSEHPGLGIEATTADSTWRLGRRGWAGWKARTGGEGKYGGYGGTVLSKNGRIVAS
FTFEDATRADAKAAVGQLKDAGVSVEMLSGDIVHACGEVAGTLDIESFVPALLPSGKVERIEVLARAGHKVLMVGDGLND
TPALAAAHVSIAPATAADIGRNAADFVFLRESLLAVPLALDVSRQAGRLIRQNIAIAIVYNAVAVPIAILGHVTPLIAAI
AMSASSLLVIGNGLRLQGFRLAELTRASSATHSGIHPSARSS
>Mature_761_residues
SCCAPSAELALDLTSTTSVLPSSQEIRLASRSLGDNLWQTDLSVPTVHCAACIQAIETALGRLDRVEGARVNLSTKRVAI
RWRGDSVPPFVAALGRLGFQAHLFEADPDKKDKTLAELIRAVAVAGFAAGNIMLLSVSVWSGAEGATRDLFHWVSALIAI
PALTFAGGIFFRSAWNALRHGRMNMDVPIAVGVSLAYAMSLYETINHGDHAYFDASVSLLFFLLIGRTLDHVMRERARTA
VKGLSQLAARGAMVLRGDGARDYLPVGEIEPGMQLLVAAGERVPVDSRVIRGASDLDCSLVSGESTPKTVASGEQVQAGT
LNLTGPLTIEATAAAKDSFLAEMVRLMEAPEGGRAHYRRIADRVSALYAPVVHLTAFVTFLGWMAVTGDWHRAVTIAIAV
LIITCPCALGLAVPIVQVVAARRLFEAGVMVKDGSAIERLAAIDVAVFDKTGTLTLGQPRLVNASTIDPAMLAIAADMAA
HSRHPFSKAIAAFAGFAGQPKLEAVSEHPGLGIEATTADSTWRLGRRGWAGWKARTGGEGKYGGYGGTVLSKNGRIVASF
TFEDATRADAKAAVGQLKDAGVSVEMLSGDIVHACGEVAGTLDIESFVPALLPSGKVERIEVLARAGHKVLMVGDGLNDT
PALAAAHVSIAPATAADIGRNAADFVFLRESLLAVPLALDVSRQAGRLIRQNIAIAIVYNAVAVPIAILGHVTPLIAAIA
MSASSLLVIGNGLRLQGFRLAELTRASSATHSGIHPSARSS

Specific function: FixI is a pump of a specific cation involved in symbiotic nitrogen fixation. The four proteins FixG, FixH, FixI, and FixS may participate in a membrane-bound complex coupling the FixI cation pump with a redox process catalyzed by FixG [H]

COG id: COG2217

COG function: function code P; Cation transport ATPase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HMA domain [H]

Homologues:

Organism=Homo sapiens, GI55743071, Length=779, Percent_Identity=26.8292682926829, Blast_Score=263, Evalue=4e-70,
Organism=Homo sapiens, GI115529486, Length=576, Percent_Identity=25.6944444444444, Blast_Score=188, Evalue=2e-47,
Organism=Homo sapiens, GI55743073, Length=728, Percent_Identity=26.3736263736264, Blast_Score=185, Evalue=2e-46,
Organism=Homo sapiens, GI48762687, Length=274, Percent_Identity=23.3576642335766, Blast_Score=71, Evalue=5e-12,
Organism=Homo sapiens, GI48762691, Length=274, Percent_Identity=23.3576642335766, Blast_Score=71, Evalue=5e-12,
Organism=Homo sapiens, GI48762689, Length=274, Percent_Identity=23.3576642335766, Blast_Score=70, Evalue=5e-12,
Organism=Homo sapiens, GI48762685, Length=274, Percent_Identity=23.3576642335766, Blast_Score=70, Evalue=6e-12,
Organism=Escherichia coli, GI1786691, Length=736, Percent_Identity=31.7934782608696, Blast_Score=313, Evalue=3e-86,
Organism=Escherichia coli, GI1789879, Length=719, Percent_Identity=29.0681502086231, Blast_Score=218, Evalue=9e-58,
Organism=Escherichia coli, GI1786914, Length=494, Percent_Identity=25.7085020242915, Blast_Score=121, Evalue=2e-28,
Organism=Escherichia coli, GI2367363, Length=263, Percent_Identity=26.9961977186312, Blast_Score=70, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17556548, Length=380, Percent_Identity=29.4736842105263, Blast_Score=165, Evalue=1e-40,
Organism=Saccharomyces cerevisiae, GI6320475, Length=629, Percent_Identity=26.8680445151033, Blast_Score=206, Evalue=1e-53,
Organism=Saccharomyces cerevisiae, GI6319772, Length=616, Percent_Identity=27.4350649350649, Blast_Score=189, Evalue=2e-48,
Organism=Saccharomyces cerevisiae, GI6321430, Length=547, Percent_Identity=24.3144424131627, Blast_Score=115, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6325221, Length=553, Percent_Identity=24.4122965641953, Blast_Score=113, Evalue=1e-25,
Organism=Drosophila melanogaster, GI221329854, Length=557, Percent_Identity=28.7253141831239, Blast_Score=184, Evalue=2e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008250
- InterPro:   IPR006403
- InterPro:   IPR000695
- InterPro:   IPR006416
- InterPro:   IPR001757
- InterPro:   IPR018303
- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR017969
- InterPro:   IPR006121
- InterPro:   IPR000150 [H]

Pfam domain/function: PF00122 E1-E2_ATPase; PF00702 Hydrolase [H]

EC number: 3.6.3.4 [C]

Molecular weight: Translated: 79998; Mature: 79867

Theoretical pI: Translated: 7.88; Mature: 7.88

Prosite motif: PS00154 ATPASE_E1_E2 ; PS01047 HMA_1 ; PS50846 HMA_2 ; PS00211 ABC_TRANSPORTER_1 ; PS01229 COF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSCCAPSAELALDLTSTTSVLPSSQEIRLASRSLGDNLWQTDLSVPTVHCAACIQAIETA
CCCCCCCCCEEEEECCCHHCCCCCHHHHHHHHHHCCHHHHCCCCCCHHHHHHHHHHHHHH
LGRLDRVEGARVNLSTKRVAIRWRGDSVPPFVAALGRLGFQAHLFEADPDKKDKTLAELI
HHHHHHCCCCEEEEEEEEEEEEECCCCCCHHHHHHHHCCCEEEEECCCCCCCHHHHHHHH
RAVAVAGFAAGNIMLLSVSVWSGAEGATRDLFHWVSALIAIPALTFAGGIFFRSAWNALR
HHHHHHHHCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HGRMNMDVPIAVGVSLAYAMSLYETINHGDHAYFDASVSLLFFLLIGRTLDHVMRERART
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHH
AVKGLSQLAARGAMVLRGDGARDYLPVGEIEPGMQLLVAAGERVPVDSRVIRGASDLDCS
HHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCHHHHEECCCCCCCCHHHHCCCCCCCEE
LVSGESTPKTVASGEQVQAGTLNLTGPLTIEATAAAKDSFLAEMVRLMEAPEGGRAHYRR
EECCCCCCCHHCCCCCEECCEEECCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCHHHHHH
IADRVSALYAPVVHLTAFVTFLGWMAVTGDWHRAVTIAIAVLIITCPCALGLAVPIVQVV
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AARRLFEAGVMVKDGSAIERLAAIDVAVFDKTGTLTLGQPRLVNASTIDPAMLAIAADMA
HHHHHHHCCCEEECCHHHHHHHHHEEEEECCCCCEECCCCCEECCCCCCHHHHHHHHHHH
AHSRHPFSKAIAAFAGFAGQPKLEAVSEHPGLGIEATTADSTWRLGRRGWAGWKARTGGE
HHCCCCHHHHHHHHHCCCCCCCHHHHHCCCCCCEEEECCCCHHHCCCCCCCCCCCCCCCC
GKYGGYGGTVLSKNGRIVASFTFEDATRADAKAAVGQLKDAGVSVEMLSGDIVHACGEVA
CCCCCCCCEEEECCCEEEEEEEECCCHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHH
GTLDIESFVPALLPSGKVERIEVLARAGHKVLMVGDGLNDTPALAAAHVSIAPATAADIG
CCCCHHHHHHHHCCCCCHHHHHHHHHCCCEEEEEECCCCCCCHHHHEEEEECCCHHHHHC
RNAADFVFLRESLLAVPLALDVSRQAGRLIRQNIAIAIVYNAVAVPIAILGHVTPLIAAI
CCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCEEEEEEHHHHHHHHHHHHHHHHHHHHH
AMSASSLLVIGNGLRLQGFRLAELTRASSATHSGIHPSARSS
HHCCCCEEEEECCCEECCEEHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SCCAPSAELALDLTSTTSVLPSSQEIRLASRSLGDNLWQTDLSVPTVHCAACIQAIETA
CCCCCCCCEEEEECCCHHCCCCCHHHHHHHHHHCCHHHHCCCCCCHHHHHHHHHHHHHH
LGRLDRVEGARVNLSTKRVAIRWRGDSVPPFVAALGRLGFQAHLFEADPDKKDKTLAELI
HHHHHHCCCCEEEEEEEEEEEEECCCCCCHHHHHHHHCCCEEEEECCCCCCCHHHHHHHH
RAVAVAGFAAGNIMLLSVSVWSGAEGATRDLFHWVSALIAIPALTFAGGIFFRSAWNALR
HHHHHHHHCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HGRMNMDVPIAVGVSLAYAMSLYETINHGDHAYFDASVSLLFFLLIGRTLDHVMRERART
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHH
AVKGLSQLAARGAMVLRGDGARDYLPVGEIEPGMQLLVAAGERVPVDSRVIRGASDLDCS
HHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCHHHHEECCCCCCCCHHHHCCCCCCCEE
LVSGESTPKTVASGEQVQAGTLNLTGPLTIEATAAAKDSFLAEMVRLMEAPEGGRAHYRR
EECCCCCCCHHCCCCCEECCEEECCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCHHHHHH
IADRVSALYAPVVHLTAFVTFLGWMAVTGDWHRAVTIAIAVLIITCPCALGLAVPIVQVV
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AARRLFEAGVMVKDGSAIERLAAIDVAVFDKTGTLTLGQPRLVNASTIDPAMLAIAADMA
HHHHHHHCCCEEECCHHHHHHHHHEEEEECCCCCEECCCCCEECCCCCCHHHHHHHHHHH
AHSRHPFSKAIAAFAGFAGQPKLEAVSEHPGLGIEATTADSTWRLGRRGWAGWKARTGGE
HHCCCCHHHHHHHHHCCCCCCCHHHHHCCCCCCEEEECCCCHHHCCCCCCCCCCCCCCCC
GKYGGYGGTVLSKNGRIVASFTFEDATRADAKAAVGQLKDAGVSVEMLSGDIVHACGEVA
CCCCCCCCEEEECCCEEEEEEEECCCHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHH
GTLDIESFVPALLPSGKVERIEVLARAGHKVLMVGDGLNDTPALAAAHVSIAPATAADIG
CCCCHHHHHHHHCCCCCHHHHHHHHHCCCEEEEEECCCCCCCHHHHEEEEECCCHHHHHC
RNAADFVFLRESLLAVPLALDVSRQAGRLIRQNIAIAIVYNAVAVPIAILGHVTPLIAAI
CCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCEEEEEEHHHHHHHHHHHHHHHHHHHHH
AMSASSLLVIGNGLRLQGFRLAELTRASSATHSGIHPSARSS
HHCCCCEEEEECCCEECCEEHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Cu [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; H2O [C]

Specific reaction: ATP + H2O = ADP + Orthophosphate. [C]

General reaction: Phosphorous acid anhydride hydrolysis [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2536685; 11481432 [H]