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The map label for this gene is traI [H]

Identifier: 13475341

GI number: 13475341

Start: 5208218

End: 5208898

Strand: Direct

Name: traI [H]

Synonym: mlr6385

Alternate gene names: 13475341

Gene position: 5208218-5208898 (Clockwise)

Preceding gene: 13475332

Following gene: 13475342

Centisome position: 74.02

GC content: 60.79

Gene sequence:

>681_bases
ATGGCGGGAACACAACCCGCACGGAGGCGACGAATGATTCAGCTGATCACACCTGGCCTCTACAGCGAATTCGCAGGCGA
GCTCAAAGAGATGCACGGGCTCAGATATCGGGTTTTCAAGGAGAGGCTCGATTGGGAAGTTCAGACCGGAGGCGAAATGG
AAACAGACACCTTTGACGACTTGAAGCCTGTCTATCTGCTGCTCAAGGGATCCGATTGGCGAATTCGTGGCTGCGTGCGC
CTCTTGCCAACCACTGGGCCGACCATGTTGCGCGACACATTCCCGGCGCTGCTGGGTGAAGCTGTGGCGCCCGCGAGTCC
CGACATATGGGAAAGCAGCCGTTTCGCGCTCGATTTGCCCCCATCAACGCCCAAGGCGGCAGGCGGCCTTGCCCAAGCAA
CCTACGAGCTCTTCGCCGGCATGATCGAATTCGGCTTGGCCAACAATCTCACCCGGATCGTGACGGTGACCGATACGCGT
ATGGAACGGATCCTTCGCCTCGCAACGTGGCCATTGTCGCGCATCGGAAAGCCGCAGCCCGTCGGCAAAACGGAGGCGGT
GGCCGGGTTTCTCGAGATCTCTCACGCCAGCCTGTTGCGCATCCGCTGGAGGGGACGTCTGAATGGGCCGGTACTGTGGC
GACCGATTCTCGGCCTGCCGCACGGCCCATGCGGTAGCTAA

Upstream 100 bases:

>100_bases
GTTGCCAGGCTGGCTGCCTCCGGTCAGGCGAGATCCGGATCCTTCCGCGAGGATAGCTGTAAACGTCCACAGGCTGCATC
GCTGTGCGCTTTGCCCTTCG

Downstream 100 bases:

>100_bases
GACGTGGTGCGTGGCCCCAGGGATCGGAAGAGAGGGTTTGCTTCTCGTCGCCGAAAGGAGTCATTGCGTCCGGCCTGGAG
AAGCAAACCGAGGAATGATG

Product: conjugation factor synthase traI

Products: NA

Alternate protein names: Autoinducer synthesis protein traI [H]

Number of amino acids: Translated: 226; Mature: 225

Protein sequence:

>226_residues
MAGTQPARRRRMIQLITPGLYSEFAGELKEMHGLRYRVFKERLDWEVQTGGEMETDTFDDLKPVYLLLKGSDWRIRGCVR
LLPTTGPTMLRDTFPALLGEAVAPASPDIWESSRFALDLPPSTPKAAGGLAQATYELFAGMIEFGLANNLTRIVTVTDTR
MERILRLATWPLSRIGKPQPVGKTEAVAGFLEISHASLLRIRWRGRLNGPVLWRPILGLPHGPCGS

Sequences:

>Translated_226_residues
MAGTQPARRRRMIQLITPGLYSEFAGELKEMHGLRYRVFKERLDWEVQTGGEMETDTFDDLKPVYLLLKGSDWRIRGCVR
LLPTTGPTMLRDTFPALLGEAVAPASPDIWESSRFALDLPPSTPKAAGGLAQATYELFAGMIEFGLANNLTRIVTVTDTR
MERILRLATWPLSRIGKPQPVGKTEAVAGFLEISHASLLRIRWRGRLNGPVLWRPILGLPHGPCGS
>Mature_225_residues
AGTQPARRRRMIQLITPGLYSEFAGELKEMHGLRYRVFKERLDWEVQTGGEMETDTFDDLKPVYLLLKGSDWRIRGCVRL
LPTTGPTMLRDTFPALLGEAVAPASPDIWESSRFALDLPPSTPKAAGGLAQATYELFAGMIEFGLANNLTRIVTVTDTRM
ERILRLATWPLSRIGKPQPVGKTEAVAGFLEISHASLLRIRWRGRLNGPVLWRPILGLPHGPCGS

Specific function: Required for the synthesis of OHHL (N-(3-oxooctanoyl)-L- homoserine lactone), an autoinducer molecule which binds to traR and thus acts in the control of conjugal transfer [H]

COG id: COG3916

COG function: function code TQ; N-acyl-L-homoserine lactone synthetase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the autoinducer synthase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016181
- InterPro:   IPR001690
- InterPro:   IPR018311 [H]

Pfam domain/function: PF00765 Autoind_synth [H]

EC number: =2.3.1.184 [H]

Molecular weight: Translated: 25165; Mature: 25033

Theoretical pI: Translated: 10.07; Mature: 10.07

Prosite motif: PS00949 AUTOINDUCERS_SYNTH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGTQPARRRRMIQLITPGLYSEFAGELKEMHGLRYRVFKERLDWEVQTGGEMETDTFDD
CCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCEEECCCCCCCCCCCCC
LKPVYLLLKGSDWRIRGCVRLLPTTGPTMLRDTFPALLGEAVAPASPDIWESSRFALDLP
CCEEEEEEECCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECC
PSTPKAAGGLAQATYELFAGMIEFGLANNLTRIVTVTDTRMERILRLATWPLSRIGKPQP
CCCCHHHCHHHHHHHHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHCCHHHCCCCCC
VGKTEAVAGFLEISHASLLRIRWRGRLNGPVLWRPILGLPHGPCGS
CCCHHHHHHHHHHCCHHEEEEEECCCCCCCEEEHHHHCCCCCCCCC
>Mature Secondary Structure 
AGTQPARRRRMIQLITPGLYSEFAGELKEMHGLRYRVFKERLDWEVQTGGEMETDTFDD
CCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCEEECCCCCCCCCCCCC
LKPVYLLLKGSDWRIRGCVRLLPTTGPTMLRDTFPALLGEAVAPASPDIWESSRFALDLP
CCEEEEEEECCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECC
PSTPKAAGGLAQATYELFAGMIEFGLANNLTRIVTVTDTRMERILRLATWPLSRIGKPQP
CCCCHHHCHHHHHHHHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHCCHHHCCCCCC
VGKTEAVAGFLEISHASLLRIRWRGRLNGPVLWRPILGLPHGPCGS
CCCHHHHHHHHHHCCHHEEEEEECCCCCCCEEEHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]