Click here to switch to the map view.

The map label for this gene is pinE [C]

Identifier: 13475288

GI number: 13475288

Start: 5136561

End: 5137091

Strand: Direct

Name: pinE [C]

Synonym: mlr6326

Alternate gene names: 13475288

Gene position: 5136561-5137091 (Clockwise)

Preceding gene: 13475287

Following gene: 13475289

Centisome position: 73.0

GC content: 62.34

Gene sequence:

>531_bases
ATGACCCGCATCGGATACGCCCGCACCTCCACCACTGACCAGAACCTCGCCGCGCAGATCGTCGCGCTCAAGGCCGCCGG
CTGCGAGGTCATTCGCGAGGAGCAGAAGAGCGGCTCTACCCTGGAAGGCCGTCCGCAACTCAACACCATTCTCGACTTCA
TTCATGCTGGCGAGACGCTTGTCGTCACCCGGATTGACCGCCTGGCCAGATCGCTGCGCGACCTGCAGATGATCGTGGAC
AAGCTCAAGACGAAGGGCGCGCATCTCTCCGCGACAGAACAACCTGTCGATACGTCGACCGCCCCCGGTAAGGCCTTCTT
TGACATGCTTGGTGTCTTTGCCGAGTTCGAGACCAATCTGCGGCGCGAGCGCCAGGCGGAGGGAATCGCTGCCGCCAAGA
AGCGCGGCACATACAAGGGCCGGCCGCCGAAAATCGACCGCGACACGATCCAGCAAAGGCTTAGACAGGGTCAGGGCCCA
CACAAATCGCCCGCGATCTCGGCGTCTCACGCGGTACTGTCTATCAGGTGA

Upstream 100 bases:

>100_bases
CCCCCATGAACACGTCGCAACCGGAAAGCCTGTATATTTTGGGCTCAATCTTCGTTAATGAACAGAGCATCGCGCCGGCA
CACCCAAAATGAACAGTTGC

Downstream 100 bases:

>100_bases
GGAAGGGCAAATGACGAAGGGCAGGCTCATAACCCGTCTGACGCCAGCGACATGCGAAGAGATCCAGACCCGCATGCTCG
AAGTCTGCCAGAAGATCGCT

Product: putative DNA invertase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 176; Mature: 175

Protein sequence:

>176_residues
MTRIGYARTSTTDQNLAAQIVALKAAGCEVIREEQKSGSTLEGRPQLNTILDFIHAGETLVVTRIDRLARSLRDLQMIVD
KLKTKGAHLSATEQPVDTSTAPGKAFFDMLGVFAEFETNLRRERQAEGIAAAKKRGTYKGRPPKIDRDTIQQRLRQGQGP
HKSPAISASHAVLSIR

Sequences:

>Translated_176_residues
MTRIGYARTSTTDQNLAAQIVALKAAGCEVIREEQKSGSTLEGRPQLNTILDFIHAGETLVVTRIDRLARSLRDLQMIVD
KLKTKGAHLSATEQPVDTSTAPGKAFFDMLGVFAEFETNLRRERQAEGIAAAKKRGTYKGRPPKIDRDTIQQRLRQGQGP
HKSPAISASHAVLSIR
>Mature_175_residues
TRIGYARTSTTDQNLAAQIVALKAAGCEVIREEQKSGSTLEGRPQLNTILDFIHAGETLVVTRIDRLARSLRDLQMIVDK
LKTKGAHLSATEQPVDTSTAPGKAFFDMLGVFAEFETNLRRERQAEGIAAAKKRGTYKGRPPKIDRDTIQQRLRQGQGPH
KSPAISASHAVLSIR

Specific function: This Protein Catalyzes The Inversion Of An 1800-Bp E.Coli DNA Fragment, The P Region, Which Can Exist In Either Orientation. The Function Of The Inversion Is Not Yet Clear. [C]

COG id: COG1961

COG function: function code L; Site-specific recombinases, DNA invertase Pin homologs

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the site-specific recombinase resolvase family [H]

Homologues:

Organism=Escherichia coli, GI1787404, Length=165, Percent_Identity=41.2121212121212, Blast_Score=116, Evalue=7e-28,
Organism=Escherichia coli, GI1787638, Length=167, Percent_Identity=32.3353293413174, Blast_Score=74, Evalue=4e-15,
Organism=Escherichia coli, GI1787827, Length=163, Percent_Identity=31.2883435582822, Blast_Score=72, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR006118
- InterPro:   IPR006119
- InterPro:   IPR006120 [H]

Pfam domain/function: PF02796 HTH_7; PF00239 Resolvase [H]

EC number: NA

Molecular weight: Translated: 19348; Mature: 19217

Theoretical pI: Translated: 10.74; Mature: 10.74

Prosite motif: PS00398 RECOMBINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRIGYARTSTTDQNLAAQIVALKAAGCEVIREEQKSGSTLEGRPQLNTILDFIHAGETL
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCH
VVTRIDRLARSLRDLQMIVDKLKTKGAHLSATEQPVDTSTAPGKAFFDMLGVFAEFETNL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
RRERQAEGIAAAKKRGTYKGRPPKIDRDTIQQRLRQGQGPHKSPAISASHAVLSIR
HHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCEEEECC
>Mature Secondary Structure 
TRIGYARTSTTDQNLAAQIVALKAAGCEVIREEQKSGSTLEGRPQLNTILDFIHAGETL
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCH
VVTRIDRLARSLRDLQMIVDKLKTKGAHLSATEQPVDTSTAPGKAFFDMLGVFAEFETNL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
RRERQAEGIAAAKKRGTYKGRPPKIDRDTIQQRLRQGQGPHKSPAISASHAVLSIR
HHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]