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The map label for this gene is proB2

Identifier: 13475267

GI number: 13475267

Start: 5114952

End: 5115806

Strand: Direct

Name: proB2

Synonym: mlr6298

Alternate gene names: 13475267

Gene position: 5114952-5115806 (Clockwise)

Preceding gene: 13475266

Following gene: 13475268

Centisome position: 72.7

GC content: 56.73

Gene sequence:

>855_bases
ATGCAAGAAAAGATTAGAGAGACATGTCTTGCAACGAAGACGGTCAAATTGCTTTCGGCTCGCCGACTGATCGTGAAGAT
TGGCTCCGCAGTGGTTGCCGATGCGGAGACCGGAGAGATACGCGGTCCTTGGCTTGAAACGTTGATAAAGGATGTCGTCC
GATTTTTCGCGCGCGGCCAGCAGGTGATCATAGTCACCTCCGGTGCTGTCGCGGCTGGATCACGTCACTTCAAGCAGTTG
GACAGATCTTTGCGAATTGAGGAGAAGCAAGCGGCCGCCGCGATTGGTCAGATCCGACTGATGATCGCTTATGAACAGAG
CTTGAAACGCCATGGGTTTGGCCTGGGGCAGGTGCTATTGACCAGCGCAGATGTAGACAACCAGCGTTGCCGCCTCAATG
CGCGGTCAGCCTTTCAACAACTCCTCAACGTTGGCGCTGTACCCGTTATCAACGAGAACGACGCGACCGCAACGCCCGAG
GTCTGTCTTGGCGACAATGATCGGCTGGCGGCAAGGGTCGCGCAAATTGCAAAGGCCGACCTTCTCATTCTGCTTTCCGA
CGTAGATGGCCTCTTTACGGAAGATCCACACGACAACCCGTTGGCTCGTATGATCCCAGAGGTGCGGCGCATTACCCCGG
AGATAGAAATCATGGCCAGCCTTTCCCCCGCGCGCCATGGTTCGGGCGGCATGGTGACAAAGTTGATGGCAGCAAGGATA
GCGATGGAAGCGGGCTGCAACGTGGTCATTGCGAAAGGAAGCAAGTCTTATCCGCTCGCCGCCATCGAGAACGGTGCGCC
ATCAACATGGTTTATTCCGCCCGCCAGGGAGACGGCAACACGCGGGGGCCGTTGA

Upstream 100 bases:

>100_bases
CATCCGAAACGTTGATGCAATTGAGAGCACGAAAGAGAGAGGCAAGTCGACGAGATGCCAAACTTTGCACCTGACCGCTC
GCGCAACTGAATGCTGTTGA

Downstream 100 bases:

>100_bases
AGAAATCCTCGGTTTGAATTGTTCGTGCAATCATCGCTTGAGGGAGCGCCTGCAGTGATAGGAGGCAGCTAGCTCAAGCA
GAGACCATCGCCGTGGTGGC

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase 2; GK 2

Number of amino acids: Translated: 284; Mature: 284

Protein sequence:

>284_residues
MQEKIRETCLATKTVKLLSARRLIVKIGSAVVADAETGEIRGPWLETLIKDVVRFFARGQQVIIVTSGAVAAGSRHFKQL
DRSLRIEEKQAAAAIGQIRLMIAYEQSLKRHGFGLGQVLLTSADVDNQRCRLNARSAFQQLLNVGAVPVINENDATATPE
VCLGDNDRLAARVAQIAKADLLILLSDVDGLFTEDPHDNPLARMIPEVRRITPEIEIMASLSPARHGSGGMVTKLMAARI
AMEAGCNVVIAKGSKSYPLAAIENGAPSTWFIPPARETATRGGR

Sequences:

>Translated_284_residues
MQEKIRETCLATKTVKLLSARRLIVKIGSAVVADAETGEIRGPWLETLIKDVVRFFARGQQVIIVTSGAVAAGSRHFKQL
DRSLRIEEKQAAAAIGQIRLMIAYEQSLKRHGFGLGQVLLTSADVDNQRCRLNARSAFQQLLNVGAVPVINENDATATPE
VCLGDNDRLAARVAQIAKADLLILLSDVDGLFTEDPHDNPLARMIPEVRRITPEIEIMASLSPARHGSGGMVTKLMAARI
AMEAGCNVVIAKGSKSYPLAAIENGAPSTWFIPPARETATRGGR
>Mature_284_residues
MQEKIRETCLATKTVKLLSARRLIVKIGSAVVADAETGEIRGPWLETLIKDVVRFFARGQQVIIVTSGAVAAGSRHFKQL
DRSLRIEEKQAAAAIGQIRLMIAYEQSLKRHGFGLGQVLLTSADVDNQRCRLNARSAFQQLLNVGAVPVINENDATATPE
VCLGDNDRLAARVAQIAKADLLILLSDVDGLFTEDPHDNPLARMIPEVRRITPEIEIMASLSPARHGSGGMVTKLMAARI
AMEAGCNVVIAKGSKSYPLAAIENGAPSTWFIPPARETATRGGR

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamate 5-kinase family

Homologues:

Organism=Homo sapiens, GI62912457, Length=269, Percent_Identity=33.457249070632, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI21361368, Length=271, Percent_Identity=33.210332103321, Blast_Score=119, Evalue=3e-27,
Organism=Escherichia coli, GI1786437, Length=236, Percent_Identity=42.7966101694915, Blast_Score=174, Evalue=6e-45,
Organism=Caenorhabditis elegans, GI17569969, Length=270, Percent_Identity=32.5925925925926, Blast_Score=124, Evalue=8e-29,
Organism=Caenorhabditis elegans, GI17569967, Length=252, Percent_Identity=33.3333333333333, Blast_Score=122, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6321822, Length=242, Percent_Identity=34.7107438016529, Blast_Score=147, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6320506, Length=233, Percent_Identity=36.0515021459227, Blast_Score=147, Evalue=3e-36,
Organism=Drosophila melanogaster, GI21357643, Length=300, Percent_Identity=31.6666666666667, Blast_Score=132, Evalue=3e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PROB2_RHILO (Q989S7)

Other databases:

- EMBL:   BA000012
- RefSeq:   NP_106831.1
- ProteinModelPortal:   Q989S7
- SMR:   Q989S7
- GeneID:   1229486
- GenomeReviews:   BA000012_GR
- KEGG:   mlo:mlr6298
- NMPDR:   fig|266835.1.peg.4932
- HOGENOM:   HBG507643
- OMA:   LINTIDP
- ProtClustDB:   PRK12314
- BRENDA:   2.7.2.11
- GO:   GO:0005737
- HAMAP:   MF_00456
- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- Gene3D:   G3DSA:3.40.1160.10
- PIRSF:   PIRSF000729
- PRINTS:   PR00474
- TIGRFAMs:   TIGR01027

Pfam domain/function: PF00696 AA_kinase; SSF53633 Aa_kinase

EC number: =2.7.2.11

Molecular weight: Translated: 30683; Mature: 30683

Theoretical pI: Translated: 9.65; Mature: 9.65

Prosite motif: PS00902 GLUTAMATE_5_KINASE; PS00213 LIPOCALIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQEKIRETCLATKTVKLLSARRLIVKIGSAVVADAETGEIRGPWLETLIKDVVRFFARGQ
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEECCCCCCCCCHHHHHHHHHHHHHHHCCC
QVIIVTSGAVAAGSRHFKQLDRSLRIEEKQAAAAIGQIRLMIAYEQSLKRHGFGLGQVLL
EEEEEECCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHEEHHHHHHHHHCCCCHHHHEE
TSADVDNQRCRLNARSAFQQLLNVGAVPVINENDATATPEVCLGDNDRLAARVAQIAKAD
EECCCCCHHHEECHHHHHHHHHHCCCCEEECCCCCCCCCCEEECCCHHHHHHHHHHHHHC
LLILLSDVDGLFTEDPHDNPLARMIPEVRRITPEIEIMASLSPARHGSGGMVTKLMAARI
EEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHH
AMEAGCNVVIAKGSKSYPLAAIENGAPSTWFIPPARETATRGGR
HHHCCCCEEEECCCCCCCEEEECCCCCCEEECCCCHHHHCCCCC
>Mature Secondary Structure
MQEKIRETCLATKTVKLLSARRLIVKIGSAVVADAETGEIRGPWLETLIKDVVRFFARGQ
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEECCCCCCCCCHHHHHHHHHHHHHHHCCC
QVIIVTSGAVAAGSRHFKQLDRSLRIEEKQAAAAIGQIRLMIAYEQSLKRHGFGLGQVLL
EEEEEECCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHEEHHHHHHHHHCCCCHHHHEE
TSADVDNQRCRLNARSAFQQLLNVGAVPVINENDATATPEVCLGDNDRLAARVAQIAKAD
EECCCCCHHHEECHHHHHHHHHHCCCCEEECCCCCCCCCCEEECCCHHHHHHHHHHHHHC
LLILLSDVDGLFTEDPHDNPLARMIPEVRRITPEIEIMASLSPARHGSGGMVTKLMAARI
EEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHH
AMEAGCNVVIAKGSKSYPLAAIENGAPSTWFIPPARETATRGGR
HHHCCCCEEEECCCCCCCEEEECCCCCCEEECCCCHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11214968