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The map label for this gene is cspA [C]

Identifier: 13475232

GI number: 13475232

Start: 5080214

End: 5080426

Strand: Direct

Name: cspA [C]

Synonym: msr6259

Alternate gene names: 13475232

Gene position: 5080214-5080426 (Clockwise)

Preceding gene: 13475231

Following gene: 13475236

Centisome position: 72.2

GC content: 56.81

Gene sequence:

>213_bases
ATGGCGATTGGAACCGTGAAATGGTTCAACAGCACGAAGGGATTTGGATTCATCCAGCCCGACAATGGCGGTCAGGATGT
TTTCGTCCACATTTCCGCTGTCGAACGCGCGGGCCTCTCCACCCTCACGGACGGCCAGAAGATCAATTATGAGGTCGAAC
AGGACCGCCGCACTGGCAAGTCTTCCGCCGGCAACCTCAGCAAAGCTGGCTGA

Upstream 100 bases:

>100_bases
TCCCGGTCTTGCGGTCTTTCGCATTTGCTGCGTTATGCTATGAACCGGCCCGGCATGATCGCCGAACCAAACGTGTGGCA
ATTTTACAAGGAATACGCAT

Downstream 100 bases:

>100_bases
ATTTTCTCCTGCGGCCAGGCAATAGTCGCGTGGGACGCGGCACATTGTTGTGACGGCGGCCGCTCGAGAAGAGTGGGCGA
GAACACGGCTCGTCCGCAGG

Product: cold shock protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 70; Mature: 69

Protein sequence:

>70_residues
MAIGTVKWFNSTKGFGFIQPDNGGQDVFVHISAVERAGLSTLTDGQKINYEVEQDRRTGKSSAGNLSKAG

Sequences:

>Translated_70_residues
MAIGTVKWFNSTKGFGFIQPDNGGQDVFVHISAVERAGLSTLTDGQKINYEVEQDRRTGKSSAGNLSKAG
>Mature_69_residues
AIGTVKWFNSTKGFGFIQPDNGGQDVFVHISAVERAGLSTLTDGQKINYEVEQDRRTGKSSAGNLSKAG

Specific function: Binds To And Stimulates The Transcription Of The CCAAT- Containing, Cold-Shock-Inducible Promoters Of The H-Ns And Gyra Proteins. Binds Also To The Inverted Repeat 5'-ATTGG-3'. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CSD (cold-shock) domain [H]

Homologues:

Organism=Escherichia coli, GI1789979, Length=64, Percent_Identity=48.4375, Blast_Score=70, Evalue=3e-14,
Organism=Escherichia coli, GI1786841, Length=49, Percent_Identity=55.1020408163265, Blast_Score=70, Evalue=4e-14,
Organism=Escherichia coli, GI2367114, Length=54, Percent_Identity=51.8518518518518, Blast_Score=69, Evalue=5e-14,
Organism=Escherichia coli, GI1788126, Length=59, Percent_Identity=50.8474576271186, Blast_Score=67, Evalue=2e-13,
Organism=Escherichia coli, GI1787107, Length=62, Percent_Identity=46.7741935483871, Blast_Score=67, Evalue=2e-13,
Organism=Escherichia coli, GI1787839, Length=50, Percent_Identity=54, Blast_Score=65, Evalue=1e-12,
Organism=Escherichia coli, GI1787834, Length=50, Percent_Identity=52, Blast_Score=63, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019844
- InterPro:   IPR012156
- InterPro:   IPR011129
- InterPro:   IPR002059
- InterPro:   IPR012340
- InterPro:   IPR016027 [H]

Pfam domain/function: PF00313 CSD [H]

EC number: NA

Molecular weight: Translated: 7489; Mature: 7358

Theoretical pI: Translated: 9.16; Mature: 9.16

Prosite motif: PS00352 COLD_SHOCK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIGTVKWFNSTKGFGFIQPDNGGQDVFVHISAVERAGLSTLTDGQKINYEVEQDRRTGK
CCCCCEEECCCCCCCEEECCCCCCCEEEEEEEECCCCCCCCCCCCCEEEEEECCCCCCCC
SSAGNLSKAG
CCCCCCCCCC
>Mature Secondary Structure 
AIGTVKWFNSTKGFGFIQPDNGGQDVFVHISAVERAGLSTLTDGQKINYEVEQDRRTGK
CCCCEEECCCCCCCEEECCCCCCCEEEEEEEECCCCCCCCCCCCCEEEEEECCCCCCCC
SSAGNLSKAG
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]