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The map label for this gene is nifS [H]

Identifier: 13474884

GI number: 13474884

Start: 4736450

End: 4737706

Strand: Reverse

Name: nifS [H]

Synonym: mll5865

Alternate gene names: 13474884

Gene position: 4737706-4736450 (Counterclockwise)

Preceding gene: 13474890

Following gene: 13474883

Centisome position: 67.33

GC content: 60.38

Gene sequence:

>1257_bases
ATGAGGCCTGTTTATCTGGATAACAACGCAACGACACGGGTCGATCCTGAAGTCGTTCAAGCGATGCTGCCGCTTTTTAC
CGACCAATTCGGCAACCCTTCGTCGAGCCACGATTTTGGCGCTTCCGCCGGGGCGGCGGTGAGAAAGACGCGCCTGCAGG
TGCAAACGCTCATCGGCGCGGAGTTCGACGACGAGATCACCTTCACCTCGGGCGGGACGGAAAGCGACAACGCGGCGATC
CTTTCAGCGCTCGAGGTGATGCCTGAGCGCACAGAGATCGTGACTTCCGCAGTCGAGCATTCGGCCGTGCTGACGCTTTG
CGCCCACCTGGAGAAGACGCGCGCCATCAAGGTGCATAGGATCCCGGTGGATCGCCACGGCCGGCTCGACCTCGACACTT
ATAAGGCCGCCCTCACCCCGCGCGTGGCGATCGTCTCGATCATGTCGGCGAACAACGAGACCGGAACGATCTTCCCGGTG
GTCGAGCTTGCTGAGTTGGCCAAAGAAGTCGGCGCCCTTTTCCATACCGACGCGGTGCAAGCGGTCGGCAAGCTTGCGAT
CGACCTGAAATCGACGGCAATCGATATGCTGTCTCTCTCCGGTCACAAATTACACGGACCGAAAGGGATCGGCGCACTTT
ATATAAAGCGCGGCGTGCGCTTCTGTCCGCTGATCAAGGGGGGAGGCCAGGAGGGCAACCGCCGCGCCGGCACGGAGAAT
ACGCCAGGCATCGTCGGCCTCGGCGTGGCAGCCGATCTTGCCTTGAACTTCATGGACGACGCGAACACACGGGTAAAGGC
GTTGCGCGACCGCCTGGAGAAGGAACTTCTCCAAAGCATCCCAGACGCCTTCGTCGCCGGCGATTTGCTAAAGCGGTTGC
CGAATACCGCAAACATCGCCTTTGGAGATATCGAAGGTGAGGGCATACTGCATTTCCTCAATCGCGAGGGCATTGCCTGC
TCCTCCGGCTCTGCTTGCGCCTGCGGCTCGCTGGAGCCGAGCCACGTCTTGGTGGCGATGAATATCCCCAATAACGCGGC
ACACGGTGCAATCCGTTTCTCCTTTTCGCGCAACAACGGCGAGGAGGACGTCGACCGGGTGCTCGAGGTCATGCCCGGGA
TCGTGAAGAAGCTGCGTGAGCTTTCCCCATCTGCCAGCCAGGCGAGAGAACTTCAATCAACTCCAGGCCTGGGCGACAAG
GCAAACCCGACAACCGTTTCCGGCCCAGGAGAATGCAATGCGCAGTTGTTTCGCTGA

Upstream 100 bases:

>100_bases
CCGGCTCCAAGAAGCGCGCGGCAACTCCTTCGGCTAGCCATCATCATGACAGACCGGAAGTGCGCTTTGCGTGATTGCGC
TCAGGAGAAGGATCACATCA

Downstream 100 bases:

>100_bases
AAGCCGCGCCATCGATGTCACCGACATCCTCGCCCGGCTGAAGAGCCTGTCGGCTGCGGAGGAGTTCTTCGCAGTCCTTG
GCATCTCTTATGATCCGAGG

Product: nitrogenase cofactor synthesis protein nifS

Products: NA

Alternate protein names: Nitrogenase metalloclusters biosynthesis protein NifS [H]

Number of amino acids: Translated: 418; Mature: 418

Protein sequence:

>418_residues
MRPVYLDNNATTRVDPEVVQAMLPLFTDQFGNPSSSHDFGASAGAAVRKTRLQVQTLIGAEFDDEITFTSGGTESDNAAI
LSALEVMPERTEIVTSAVEHSAVLTLCAHLEKTRAIKVHRIPVDRHGRLDLDTYKAALTPRVAIVSIMSANNETGTIFPV
VELAELAKEVGALFHTDAVQAVGKLAIDLKSTAIDMLSLSGHKLHGPKGIGALYIKRGVRFCPLIKGGGQEGNRRAGTEN
TPGIVGLGVAADLALNFMDDANTRVKALRDRLEKELLQSIPDAFVAGDLLKRLPNTANIAFGDIEGEGILHFLNREGIAC
SSGSACACGSLEPSHVLVAMNIPNNAAHGAIRFSFSRNNGEEDVDRVLEVMPGIVKKLRELSPSASQARELQSTPGLGDK
ANPTTVSGPGECNAQLFR

Sequences:

>Translated_418_residues
MRPVYLDNNATTRVDPEVVQAMLPLFTDQFGNPSSSHDFGASAGAAVRKTRLQVQTLIGAEFDDEITFTSGGTESDNAAI
LSALEVMPERTEIVTSAVEHSAVLTLCAHLEKTRAIKVHRIPVDRHGRLDLDTYKAALTPRVAIVSIMSANNETGTIFPV
VELAELAKEVGALFHTDAVQAVGKLAIDLKSTAIDMLSLSGHKLHGPKGIGALYIKRGVRFCPLIKGGGQEGNRRAGTEN
TPGIVGLGVAADLALNFMDDANTRVKALRDRLEKELLQSIPDAFVAGDLLKRLPNTANIAFGDIEGEGILHFLNREGIAC
SSGSACACGSLEPSHVLVAMNIPNNAAHGAIRFSFSRNNGEEDVDRVLEVMPGIVKKLRELSPSASQARELQSTPGLGDK
ANPTTVSGPGECNAQLFR
>Mature_418_residues
MRPVYLDNNATTRVDPEVVQAMLPLFTDQFGNPSSSHDFGASAGAAVRKTRLQVQTLIGAEFDDEITFTSGGTESDNAAI
LSALEVMPERTEIVTSAVEHSAVLTLCAHLEKTRAIKVHRIPVDRHGRLDLDTYKAALTPRVAIVSIMSANNETGTIFPV
VELAELAKEVGALFHTDAVQAVGKLAIDLKSTAIDMLSLSGHKLHGPKGIGALYIKRGVRFCPLIKGGGQEGNRRAGTEN
TPGIVGLGVAADLALNFMDDANTRVKALRDRLEKELLQSIPDAFVAGDLLKRLPNTANIAFGDIEGEGILHFLNREGIAC
SSGSACACGSLEPSHVLVAMNIPNNAAHGAIRFSFSRNNGEEDVDRVLEVMPGIVKKLRELSPSASQARELQSTPGLGDK
ANPTTVSGPGECNAQLFR

Specific function: Catalyzes the removal of elemental sulfur atoms from cysteine to produce alanine. Seems to participate in the biosynthesis of the nitrogenase metalloclusters by providing the inorganic sulfur required for the Fe-S core formation [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=387, Percent_Identity=42.6356589147287, Blast_Score=305, Evalue=6e-83,
Organism=Homo sapiens, GI156713448, Length=416, Percent_Identity=34.8557692307692, Blast_Score=211, Evalue=8e-55,
Organism=Escherichia coli, GI48994898, Length=387, Percent_Identity=42.1188630490956, Blast_Score=281, Evalue=6e-77,
Organism=Escherichia coli, GI1787970, Length=387, Percent_Identity=28.1653746770026, Blast_Score=109, Evalue=3e-25,
Organism=Escherichia coli, GI1789175, Length=397, Percent_Identity=26.7002518891688, Blast_Score=99, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI25143064, Length=394, Percent_Identity=39.8477157360406, Blast_Score=269, Evalue=3e-72,
Organism=Caenorhabditis elegans, GI17533177, Length=313, Percent_Identity=30.6709265175719, Blast_Score=148, Evalue=6e-36,
Organism=Saccharomyces cerevisiae, GI6319831, Length=394, Percent_Identity=41.3705583756345, Blast_Score=271, Evalue=1e-73,
Organism=Drosophila melanogaster, GI20129463, Length=393, Percent_Identity=42.2391857506361, Blast_Score=287, Evalue=1e-77,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR017772
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 44487; Mature: 44487

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPVYLDNNATTRVDPEVVQAMLPLFTDQFGNPSSSHDFGASAGAAVRKTRLQVQTLIGA
CCCEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCC
EFDDEITFTSGGTESDNAAILSALEVMPERTEIVTSAVEHSAVLTLCAHLEKTRAIKVHR
CCCCCEEEECCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEE
IPVDRHGRLDLDTYKAALTPRVAIVSIMSANNETGTIFPVVELAELAKEVGALFHTDAVQ
CCCCCCCCCCHHHHHHHHCCHHEEEEEEECCCCCCEEEHHHHHHHHHHHHHHHHHHHHHH
AVGKLAIDLKSTAIDMLSLSGHKLHGPKGIGALYIKRGVRFCPLIKGGGQEGNRRAGTEN
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCCEEEEECCCCCCCCCCCCCCC
TPGIVGLGVAADLALNFMDDANTRVKALRDRLEKELLQSIPDAFVAGDLLKRLPNTANIA
CCCEEEECHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCEE
FGDIEGEGILHFLNREGIACSSGSACACGSLEPSHVLVAMNIPNNAAHGAIRFSFSRNNG
EECCCCCCCEEEECCCCCEECCCCCCEECCCCCCEEEEEEECCCCCCCCEEEEEEECCCC
EEDVDRVLEVMPGIVKKLRELSPSASQARELQSTPGLGDKANPTTVSGPGECNAQLFR
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCEECCCCCCCCCCCC
>Mature Secondary Structure
MRPVYLDNNATTRVDPEVVQAMLPLFTDQFGNPSSSHDFGASAGAAVRKTRLQVQTLIGA
CCCEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCC
EFDDEITFTSGGTESDNAAILSALEVMPERTEIVTSAVEHSAVLTLCAHLEKTRAIKVHR
CCCCCEEEECCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEE
IPVDRHGRLDLDTYKAALTPRVAIVSIMSANNETGTIFPVVELAELAKEVGALFHTDAVQ
CCCCCCCCCCHHHHHHHHCCHHEEEEEEECCCCCCEEEHHHHHHHHHHHHHHHHHHHHHH
AVGKLAIDLKSTAIDMLSLSGHKLHGPKGIGALYIKRGVRFCPLIKGGGQEGNRRAGTEN
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCCEEEEECCCCCCCCCCCCCCC
TPGIVGLGVAADLALNFMDDANTRVKALRDRLEKELLQSIPDAFVAGDLLKRLPNTANIA
CCCEEEECHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCEE
FGDIEGEGILHFLNREGIACSSGSACACGSLEPSHVLVAMNIPNNAAHGAIRFSFSRNNG
EECCCCCCCEEEECCCCCEECCCCCCEECCCCCCEEEEEEECCCCCCCCEEEEEEECCCC
EEDVDRVLEVMPGIVKKLRELSPSASQARELQSTPGLGDKANPTTVSGPGECNAQLFR
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]