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The map label for this gene is queD [H]

Identifier: 13474825

GI number: 13474825

Start: 4675038

End: 4675397

Strand: Reverse

Name: queD [H]

Synonym: mll5797

Alternate gene names: 13474825

Gene position: 4675397-4675038 (Counterclockwise)

Preceding gene: 13474826

Following gene: 13474824

Centisome position: 66.45

GC content: 58.33

Gene sequence:

>360_bases
GTGATGTTCCGCATTACCAAAGAGTTCCACTTCTCGGCCTCGCATCAGCTCACCTCCTTGCCACCAGATCATCAGTGCGC
ACGTCTGCACGGCCACAACTACATCGTCGTAGTGGAGCTTTCAGGTGGCGAACTGGACGAACACGGCTTTGTGCGCGACT
ACCAAGATCTGGCGGCGTTCAAGCACTACATCGATGGGACGTTCGACCATCGGCATCTTAACGACGTGCTGGGGCATGAC
CATGCCACAGCGGAATGTCTGGCCAGGCACTTCTACGACTGGTGCAAGGTGCGGCTACCCGAAACCTCCGCCGTGCGGGT
GAGCGAGACGCCGAAAACCTGGGCGGAATACCGACCATGA

Upstream 100 bases:

>100_bases
GCGTCGAACGGCGCGAAGCATTCGATCTGGCCGGCATCACCGACCCGACAGATTATGAGGACGCAGACTTCTGGCTCCAC
GCTATCCAGACAAGGAGCGC

Downstream 100 bases:

>100_bases
CTTGCGCGTTGCATCCCGGAATCCGGGTGAGCGAGATTTTTGGGCCGACCATTCAGGGCGAAGGCGTGCTGATCGGCTTG
CCGACGGTGTTCGTAAGAAC

Product: 6-pyruvoyl tetrahydrobiopterin synthase

Products: 6-pyruvoyl-5,6,7,8-tetrahydropterin; triphosphate

Alternate protein names: CPH4 synthase; Queuosine biosynthesis protein queD [H]

Number of amino acids: Translated: 119; Mature: 119

Protein sequence:

>119_residues
MMFRITKEFHFSASHQLTSLPPDHQCARLHGHNYIVVVELSGGELDEHGFVRDYQDLAAFKHYIDGTFDHRHLNDVLGHD
HATAECLARHFYDWCKVRLPETSAVRVSETPKTWAEYRP

Sequences:

>Translated_119_residues
MMFRITKEFHFSASHQLTSLPPDHQCARLHGHNYIVVVELSGGELDEHGFVRDYQDLAAFKHYIDGTFDHRHLNDVLGHD
HATAECLARHFYDWCKVRLPETSAVRVSETPKTWAEYRP
>Mature_119_residues
MMFRITKEFHFSASHQLTSLPPDHQCARLHGHNYIVVVELSGGELDEHGFVRDYQDLAAFKHYIDGTFDHRHLNDVLGHD
HATAECLARHFYDWCKVRLPETSAVRVSETPKTWAEYRP

Specific function: Catalyzes the conversion of 7,8-dihydroneopterin triphosphate (H2NTP) to 6-carboxy-5,6,7,8-tetrahydropterin (CPH4) and acetaldehyde [H]

COG id: COG0720

COG function: function code H; 6-pyruvoyl-tetrahydropterin synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PTPS family. QueD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789124, Length=120, Percent_Identity=40.8333333333333, Blast_Score=83, Evalue=6e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007116
- InterPro:   IPR007115 [H]

Pfam domain/function: PF01242 PTPS [H]

EC number: 4.2.3.12

Molecular weight: Translated: 13860; Mature: 13860

Theoretical pI: Translated: 6.64; Mature: 6.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMFRITKEFHFSASHQLTSLPPDHQCARLHGHNYIVVVELSGGELDEHGFVRDYQDLAAF
CCEEECCCCCCCCCCCCCCCCCCCCHHEECCCCEEEEEEECCCCCCCCCCHHHHHHHHHH
KHYIDGTFDHRHLNDVLGHDHATAECLARHFYDWCKVRLPETSAVRVSETPKTWAEYRP
HHHHCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCC
>Mature Secondary Structure
MMFRITKEFHFSASHQLTSLPPDHQCARLHGHNYIVVVELSGGELDEHGFVRDYQDLAAF
CCEEECCCCCCCCCCCCCCCCCCCCHHEECCCCEEEEEEECCCCCCCCCCHHHHHHHHHH
KHYIDGTFDHRHLNDVLGHDHATAECLARHFYDWCKVRLPETSAVRVSETPKTWAEYRP
HHHHCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Mg2+; Zn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 17.7 {6-(L-erythro-1,2-dihydroxypropyl} 5 {6-(L-erythro-1,2-dihydroxypropyl} 0.0091 {6-(L-erythro-1,2-dihydroxypropyl} 0.008 {6-(L-erythro-1,2-dihydroxypropyl} [C]

Substrates: 6-[(1S,2R)-1,2-dihydroxy-3-triphosphooxypropyl]-7,8-dihydropterin

Specific reaction: 6-[(1S,2R)-1,2-dihydroxy-3-triphosphooxypropyl]-7,8-dihydropterin = 6-pyruvoyl-5,6,7,8-tetrahydropterin + triphosphate

General reaction: Intramolecular redox reaction; P-O bond cleavage; Triphosphate elimination [C]

Inhibitor: (NH4)2SO4 [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]