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The map label for this gene is queE [H]

Identifier: 13474824

GI number: 13474824

Start: 4674304

End: 4675041

Strand: Reverse

Name: queE [H]

Synonym: mll5796

Alternate gene names: 13474824

Gene position: 4675041-4674304 (Counterclockwise)

Preceding gene: 13474825

Following gene: 13474823

Centisome position: 66.44

GC content: 60.16

Gene sequence:

>738_bases
ATGACTTGCGCGTTGCATCCCGGAATCCGGGTGAGCGAGATTTTTGGGCCGACCATTCAGGGCGAAGGCGTGCTGATCGG
CTTGCCGACGGTGTTCGTAAGAACCGGCGGGTGCGATTATCGCTGTTCCTGGTGCGACAGCCTGCATGCGGTGGACCGTC
GCTTCCGCCATGATTGGGAGATGATGTCTTCCGACGCGGTTTGGCAAAAGGTCATTGCACTTTCCAGCGGTCAGCCCGTG
ATGGTATCGCTGTCGGGCGGCAATCCGGCCATCCAGCCGCTTGGCCAGTTGATCGACCGCGGGCATGGTGAGGGCTACCG
TTTTGCATTGGAAACCCAAGGTTCGGTGTCTAAGCAATGGTTCGCGGATCTCGACGTGCTGGTACTCAGCCCCAAGCCGC
CCTCAAGTGAAATGACGACAGATTGGGCCGCGTTCGACGCCTGCTTAGAGGCGGCGCAGGATAAGCCGCAGACGGCGCTC
AAGCTCGTCGTCTTCGACGAAGATGACTATGCCTATGCCAAAGACGCCGCGGCGCGCTACCCGCAATTACCCGTCTATCT
GCAGCCCGGCAATCACACGCCGCCGCGTCCCGGCAACGAAGACGGGTTCATCGACATGGCCGGTGTGATGAGGCGCATGG
AATGGCTGGTTGAAAAAGTGACCCGTGACAGGTGGTTCGAGGCGCGTGTACTGCCGCAGCTTCATGTTCTGCTCTGGGGA
AACAAACGGGGCGTCTAG

Upstream 100 bases:

>100_bases
GTCTGGCCAGGCACTTCTACGACTGGTGCAAGGTGCGGCTACCCGAAACCTCCGCCGTGCGGGTGAGCGAGACGCCGAAA
ACCTGGGCGGAATACCGACC

Downstream 100 bases:

>100_bases
CCGAAGGGGAAGGATTGCCGACGACGAGCAAGACAGACCTCTATAGCAATCTCACCCAACTGGGCGGCAGCAGCGTCGTT
CCGGAAAGCCCCGAAAACAC

Product: hypothetical protein

Products: NA

Alternate protein names: CDG synthase; Queuosine biosynthesis protein queE [H]

Number of amino acids: Translated: 245; Mature: 244

Protein sequence:

>245_residues
MTCALHPGIRVSEIFGPTIQGEGVLIGLPTVFVRTGGCDYRCSWCDSLHAVDRRFRHDWEMMSSDAVWQKVIALSSGQPV
MVSLSGGNPAIQPLGQLIDRGHGEGYRFALETQGSVSKQWFADLDVLVLSPKPPSSEMTTDWAAFDACLEAAQDKPQTAL
KLVVFDEDDYAYAKDAAARYPQLPVYLQPGNHTPPRPGNEDGFIDMAGVMRRMEWLVEKVTRDRWFEARVLPQLHVLLWG
NKRGV

Sequences:

>Translated_245_residues
MTCALHPGIRVSEIFGPTIQGEGVLIGLPTVFVRTGGCDYRCSWCDSLHAVDRRFRHDWEMMSSDAVWQKVIALSSGQPV
MVSLSGGNPAIQPLGQLIDRGHGEGYRFALETQGSVSKQWFADLDVLVLSPKPPSSEMTTDWAAFDACLEAAQDKPQTAL
KLVVFDEDDYAYAKDAAARYPQLPVYLQPGNHTPPRPGNEDGFIDMAGVMRRMEWLVEKVTRDRWFEARVLPQLHVLLWG
NKRGV
>Mature_244_residues
TCALHPGIRVSEIFGPTIQGEGVLIGLPTVFVRTGGCDYRCSWCDSLHAVDRRFRHDWEMMSSDAVWQKVIALSSGQPVM
VSLSGGNPAIQPLGQLIDRGHGEGYRFALETQGSVSKQWFADLDVLVLSPKPPSSEMTTDWAAFDACLEAAQDKPQTALK
LVVFDEDDYAYAKDAAARYPQLPVYLQPGNHTPPRPGNEDGFIDMAGVMRRMEWLVEKVTRDRWFEARVLPQLHVLLWGN
KRGV

Specific function: Catalyzes the conversion of 6-carboxy-5,6,7,8- tetrahydropterin (CPH4) to 7-carboxy-7-deazaguanine (CDG) [H]

COG id: COG0602

COG function: function code O; Organic radical activating enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. 7-carboxy-7- deazaguanine synthase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017742
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 27316; Mature: 27185

Theoretical pI: Translated: 5.62; Mature: 5.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTCALHPGIRVSEIFGPTIQGEGVLIGLPTVFVRTGGCDYRCSWCDSLHAVDRRFRHDWE
CCEECCCCCEEHHHCCCCCCCCEEEEECCEEEEEECCCCEEEHHHHHHHHHHHHHHHHHH
MMSSDAVWQKVIALSSGQPVMVSLSGGNPAIQPLGQLIDRGHGEGYRFALETQGSVSKQW
HHCCHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHCCCCCCEEEEEECCCCCCHHH
FADLDVLVLSPKPPSSEMTTDWAAFDACLEAAQDKPQTALKLVVFDEDDYAYAKDAAARY
HCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHCC
PQLPVYLQPGNHTPPRPGNEDGFIDMAGVMRRMEWLVEKVTRDRWFEARVLPQLHVLLWG
CCCCEEECCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEC
NKRGV
CCCCC
>Mature Secondary Structure 
TCALHPGIRVSEIFGPTIQGEGVLIGLPTVFVRTGGCDYRCSWCDSLHAVDRRFRHDWE
CEECCCCCEEHHHCCCCCCCCEEEEECCEEEEEECCCCEEEHHHHHHHHHHHHHHHHHH
MMSSDAVWQKVIALSSGQPVMVSLSGGNPAIQPLGQLIDRGHGEGYRFALETQGSVSKQW
HHCCHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHCCCCCCEEEEEECCCCCCHHH
FADLDVLVLSPKPPSSEMTTDWAAFDACLEAAQDKPQTALKLVVFDEDDYAYAKDAAARY
HCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHCC
PQLPVYLQPGNHTPPRPGNEDGFIDMAGVMRRMEWLVEKVTRDRWFEARVLPQLHVLLWG
CCCCEEECCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEC
NKRGV
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]