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The map label for this gene is thiG

Identifier: 13474820

GI number: 13474820

Start: 4670028

End: 4670801

Strand: Reverse

Name: thiG

Synonym: mll5790

Alternate gene names: 13474820

Gene position: 4670801-4670028 (Counterclockwise)

Preceding gene: 13474821

Following gene: 13474819

Centisome position: 66.38

GC content: 64.86

Gene sequence:

>774_bases
ATGTTCGAGCTTCTCGGGACGACGCTTCCTTCCCGCCTGCTTCTCGGCACCGCGCAATATCCTTCGCCGGCCATCCTTGC
CGATGCGGTCAAGGCATCGGGCACGTCGGTGGTGACCGTCTCGCTGCGCCGTGAGATGGCAGGTGGCAGGGCCGGCGAGA
AATTCTGGTCGTTGATCCGCTCGCTGGGCGTCAGAATCCTCCCCAACACTGCTGGCTGTCACTCCGTCAAGGAAGCGGTC
ACCACTGCGAAAATGGCGCGCGACGTCTTCGGTACGAGCTGGATCAAGCTCGAAGTGATCGGCAGCCACGACACGCTGCA
GCCAGACGTGTTCGGCCTGGTCGAAGGCGCCCGCATCCTGTGCGAAGATGGCTTTTCGGTATTCCCCTATACCACCGACG
ACCTTGTCGTGGCCGAACGGCTGCTGGAGGCGGGCTGCAGGGTCTTGATGCCGTGGTGCGCGCCGATCGGTTCCGCCCTC
GGACCGGTCAACATGACGGCACTGCGCTCGATGCGCGGACATTTCCCTGACGTCCCGCTGATCGTGGATGCAGGCCTCGG
GCGACCGTCACACGCCGCAACGGTCATGGAGCTCGGTTTCGACGCCGTGCTCCTGAACACGGCGGTCGCCAGGGCGGCCG
ATCCGGTCGGCATGGCGCGTGCGTTCGGCAAGGCGGTCGATGCCGGTCGCGAAGCCTTCAGTTCAGGATTGCTCGAGCCA
CGCGAGGTCGCCGTGCCGTCGACGCCGATATTCGGCAGGGCGGTTTTCTCATGA

Upstream 100 bases:

>100_bases
GCCGTCAACAGCGACCTCGTGCACAAAGCCAGCCGGGCGGAATTCCGGTTGAGCGACGGCGATCGGATCGAAATCCTCTC
GCCCATGCAGGGAGGCTAGC

Downstream 100 bases:

>100_bases
AGCTCGATCCGTTCTACCTAATCGTCGACAGCGCAGCCTGGATCGAACGGCTGCTGCCGCTCGGCGTCAAGCTGGTGCAG
CTGCGTATCAAGACCAAGGA

Product: thiazole synthase

Products: 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate; 4-hydroxy-benzyl-alcohol; C1 of tyrosine; ThiS protein [C]

Alternate protein names: NA

Number of amino acids: Translated: 257; Mature: 257

Protein sequence:

>257_residues
MFELLGTTLPSRLLLGTAQYPSPAILADAVKASGTSVVTVSLRREMAGGRAGEKFWSLIRSLGVRILPNTAGCHSVKEAV
TTAKMARDVFGTSWIKLEVIGSHDTLQPDVFGLVEGARILCEDGFSVFPYTTDDLVVAERLLEAGCRVLMPWCAPIGSAL
GPVNMTALRSMRGHFPDVPLIVDAGLGRPSHAATVMELGFDAVLLNTAVARAADPVGMARAFGKAVDAGREAFSSGLLEP
REVAVPSTPIFGRAVFS

Sequences:

>Translated_257_residues
MFELLGTTLPSRLLLGTAQYPSPAILADAVKASGTSVVTVSLRREMAGGRAGEKFWSLIRSLGVRILPNTAGCHSVKEAV
TTAKMARDVFGTSWIKLEVIGSHDTLQPDVFGLVEGARILCEDGFSVFPYTTDDLVVAERLLEAGCRVLMPWCAPIGSAL
GPVNMTALRSMRGHFPDVPLIVDAGLGRPSHAATVMELGFDAVLLNTAVARAADPVGMARAFGKAVDAGREAFSSGLLEP
REVAVPSTPIFGRAVFS
>Mature_257_residues
MFELLGTTLPSRLLLGTAQYPSPAILADAVKASGTSVVTVSLRREMAGGRAGEKFWSLIRSLGVRILPNTAGCHSVKEAV
TTAKMARDVFGTSWIKLEVIGSHDTLQPDVFGLVEGARILCEDGFSVFPYTTDDLVVAERLLEAGCRVLMPWCAPIGSAL
GPVNMTALRSMRGHFPDVPLIVDAGLGRPSHAATVMELGFDAVLLNTAVARAADPVGMARAFGKAVDAGREAFSSGLLEP
REVAVPSTPIFGRAVFS

Specific function: Catalyzes the rearrangement of 1-deoxy-D-xylulose 5- phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H(2)S

COG id: COG2022

COG function: function code H; Uncharacterized enzyme of thiazole biosynthesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiG family

Homologues:

Organism=Escherichia coli, GI48994993, Length=236, Percent_Identity=41.1016949152542, Blast_Score=169, Evalue=2e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIG_RHILO (Q98AZ6)

Other databases:

- EMBL:   BA000012
- RefSeq:   NP_106390.1
- ProteinModelPortal:   Q98AZ6
- SMR:   Q98AZ6
- GeneID:   1229049
- GenomeReviews:   BA000012_GR
- KEGG:   mlo:mll5790
- NMPDR:   fig|266835.1.peg.4493
- HOGENOM:   HBG296821
- OMA:   VAIRRTN
- ProtClustDB:   PRK00208
- GO:   GO:0005737
- HAMAP:   MF_00443
- InterPro:   IPR013785
- InterPro:   IPR008867
- Gene3D:   G3DSA:3.20.20.70

Pfam domain/function: PF05690 ThiG; SSF110399 ThiG

EC number: NA

Molecular weight: Translated: 27123; Mature: 27123

Theoretical pI: Translated: 6.92; Mature: 6.92

Prosite motif: NA

Important sites: ACT_SITE 96-96 BINDING 157-157

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFELLGTTLPSRLLLGTAQYPSPAILADAVKASGTSVVTVSLRREMAGGRAGEKFWSLIR
CCCCCCCCCCHHHHCCCCCCCCCHHHHHHHHCCCCEEEEEEEHHHHCCCCCHHHHHHHHH
SLGVRILPNTAGCHSVKEAVTTAKMARDVFGTSWIKLEVIGSHDTLQPDVFGLVEGARIL
HCCCEECCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHCCCEE
CEDGFSVFPYTTDDLVVAERLLEAGCRVLMPWCAPIGSALGPVNMTALRSMRGHFPDVPL
ECCCCEECCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCE
IVDAGLGRPSHAATVMELGFDAVLLNTAVARAADPVGMARAFGKAVDAGREAFSSGLLEP
EEECCCCCCHHHHHHHHHCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCC
REVAVPSTPIFGRAVFS
HHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MFELLGTTLPSRLLLGTAQYPSPAILADAVKASGTSVVTVSLRREMAGGRAGEKFWSLIR
CCCCCCCCCCHHHHCCCCCCCCCHHHHHHHHCCCCEEEEEEEHHHHCCCCCHHHHHHHHH
SLGVRILPNTAGCHSVKEAVTTAKMARDVFGTSWIKLEVIGSHDTLQPDVFGLVEGARIL
HCCCEECCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHCCCEE
CEDGFSVFPYTTDDLVVAERLLEAGCRVLMPWCAPIGSALGPVNMTALRSMRGHFPDVPL
ECCCCEECCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCE
IVDAGLGRPSHAATVMELGFDAVLLNTAVARAADPVGMARAFGKAVDAGREAFSSGLLEP
EEECCCCCCHHHHHHHHHCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCC
REVAVPSTPIFGRAVFS
HHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxyxylulose-5-phosphate; ThiS-COSH; L-tyrosine [C]

Specific reaction: deoxyxylulose-5-phosphate + ThiS-COSH + L-tyrosine = 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate + 4-hydroxy-benzyl-alcohol + C1 of tyrosine + ThiS protein [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11214968