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The map label for this gene is thiE [H]

Identifier: 13474819

GI number: 13474819

Start: 4669426

End: 4670031

Strand: Reverse

Name: thiE [H]

Synonym: mll5789

Alternate gene names: 13474819

Gene position: 4670031-4669426 (Counterclockwise)

Preceding gene: 13474820

Following gene: 13474818

Centisome position: 66.37

GC content: 62.05

Gene sequence:

>606_bases
ATGAAGCTCGATCCGTTCTACCTAATCGTCGACAGCGCAGCCTGGATCGAACGGCTGCTGCCGCTCGGCGTCAAGCTGGT
GCAGCTGCGTATCAAGACCAAGGATGAGGCCGGGTTGCGCGCGGAAATCCGGAAGGCAACGGCCTTGTGCGCCGAGCACC
AAAGCCAGCTCATCGTCAATGACCATTGGCGCCTGGCAATCGAGGAAGGCTGCAACTTCGTCCATCTCGGCCAGGAGGAT
TTGCAGACCGCCGACCGCGCGCGGATACGGGCAGCCGGCGTCAGGCTCGGGCTGAGCACGCATGATCATGTCGAACTGGA
GACGGCCTTGTTTGCCGAGCCCGATTACATCGCGCTCGGTCCCATCTACCCAACCATCCTGAAGAAGATGAAATGGGCGC
CGCAAGGGCTCAAACGAATCCGCGAGTGGAAGCGCCGGGTCGCACCTGTCCCATTGGTCGCCATTGGAGGCCTCAATCCT
GACCGGCTCGACGGTGTCTTTGCGGCTGGCGTGGACAGCGCGGCCGTCGTGACCGACATCACGCTGAATGCCGATCCCGA
AGCGCGCACACGAGAATGGATCGAAAAGACGGAGCGATGGCGCTGA

Upstream 100 bases:

>100_bases
AGGCGGTCGATGCCGGTCGCGAAGCCTTCAGTTCAGGATTGCTCGAGCCACGCGAGGTCGCCGTGCCGTCGACGCCGATA
TTCGGCAGGGCGGTTTTCTC

Downstream 100 bases:

>100_bases
GGCGAGAACCGCATGTGCTTGTCGTTGGCGGGTCGGACTCCAGCGGTGGAGCTGGAATTGCCCGCGATATAGAAACGGTT
TCCGCCTTCGGGGTGCGAAG

Product: thiamine-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase [H]

Number of amino acids: Translated: 201; Mature: 201

Protein sequence:

>201_residues
MKLDPFYLIVDSAAWIERLLPLGVKLVQLRIKTKDEAGLRAEIRKATALCAEHQSQLIVNDHWRLAIEEGCNFVHLGQED
LQTADRARIRAAGVRLGLSTHDHVELETALFAEPDYIALGPIYPTILKKMKWAPQGLKRIREWKRRVAPVPLVAIGGLNP
DRLDGVFAAGVDSAAVVTDITLNADPEARTREWIEKTERWR

Sequences:

>Translated_201_residues
MKLDPFYLIVDSAAWIERLLPLGVKLVQLRIKTKDEAGLRAEIRKATALCAEHQSQLIVNDHWRLAIEEGCNFVHLGQED
LQTADRARIRAAGVRLGLSTHDHVELETALFAEPDYIALGPIYPTILKKMKWAPQGLKRIREWKRRVAPVPLVAIGGLNP
DRLDGVFAAGVDSAAVVTDITLNADPEARTREWIEKTERWR
>Mature_201_residues
MKLDPFYLIVDSAAWIERLLPLGVKLVQLRIKTKDEAGLRAEIRKATALCAEHQSQLIVNDHWRLAIEEGCNFVHLGQED
LQTADRARIRAAGVRLGLSTHDHVELETALFAEPDYIALGPIYPTILKKMKWAPQGLKRIREWKRRVAPVPLVAIGGLNP
DRLDGVFAAGVDSAAVVTDITLNADPEARTREWIEKTERWR

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP) [H]

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family [H]

Homologues:

Organism=Escherichia coli, GI1790426, Length=190, Percent_Identity=48.9473684210526, Blast_Score=171, Evalue=3e-44,
Organism=Saccharomyces cerevisiae, GI6325042, Length=214, Percent_Identity=29.4392523364486, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF02581 TMP-TENI [H]

EC number: =2.5.1.3 [H]

Molecular weight: Translated: 22656; Mature: 22656

Theoretical pI: Translated: 8.24; Mature: 8.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLDPFYLIVDSAAWIERLLPLGVKLVQLRIKTKDEAGLRAEIRKATALCAEHQSQLIVN
CCCCCEEEEECCHHHHHHHHHCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCEEEC
DHWRLAIEEGCNFVHLGQEDLQTADRARIRAAGVRLGLSTHDHVELETALFAEPDYIALG
CCEEEEHHHCCCEEEECHHHHHHHHHHHHHHHHEEECCCCCCCEEEHHHEECCCCEEEEC
PIYPTILKKMKWAPQGLKRIREWKRRVAPVPLVAIGGLNPDRLDGVFAAGVDSAAVVTDI
CHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCEEEECCCCHHHHCCEEECCCCCCEEEEEE
TLNADPEARTREWIEKTERWR
EECCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MKLDPFYLIVDSAAWIERLLPLGVKLVQLRIKTKDEAGLRAEIRKATALCAEHQSQLIVN
CCCCCEEEEECCHHHHHHHHHCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCEEEC
DHWRLAIEEGCNFVHLGQEDLQTADRARIRAAGVRLGLSTHDHVELETALFAEPDYIALG
CCEEEEHHHCCCEEEECHHHHHHHHHHHHHHHHEEECCCCCCCEEEHHHEECCCCEEEEC
PIYPTILKKMKWAPQGLKRIREWKRRVAPVPLVAIGGLNPDRLDGVFAAGVDSAAVVTDI
CHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCEEEECCCCHHHHCCEEECCCCCCEEEEEE
TLNADPEARTREWIEKTERWR
EECCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9371431 [H]