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The map label for this gene is pyrD

Identifier: 13474728

GI number: 13474728

Start: 4568317

End: 4569396

Strand: Reverse

Name: pyrD

Synonym: mll5681

Alternate gene names: 13474728

Gene position: 4569396-4568317 (Counterclockwise)

Preceding gene: 13474729

Following gene: 13474726

Centisome position: 64.94

GC content: 66.57

Gene sequence:

>1080_bases
ATGAGCGTGCTCGACCGACTCGGCCAGAAGCTGCTGTTTGCCTTCGATCCGGAAACCGCGCACGGCCTGTCGATCGCGGC
GCTGCGCTGCGGCCTGCCGGCCGGCACGCGGGCGGTGCGCGACATCAGGCTGAAGGTCAGCCTCTGCGGTCTCGATTTCC
CCAACCCGCTCGGCATGGCGGCCGGCTATGACAAGGACGCCGAGGTGCCCGACGCACTGCTCGGCCTCGGCTTCGGCTTT
GCCGAGGTCGGCACGGTCACGCCGCTGCCGCAGGCCGGCAACCCGAAGCCGCGCATCTTCCGGCTAACGGCGGATGAAGC
GGTGATCAACCGGCTGGGTTTCAACAATGAAGGCCATGCGGCGGCTGAAAAACGCCTTGCCGCGCGCAAGGGGCGCCCCG
GCATTGTCGGCGTCAACATCGGTGCCAACAAGGACAGCGCCGATCGCATCGGCGACTATGAGCGCGGTGTCGGCCGCTTC
GCGCGCCACGCCAGCTATTTGACCGTCAACATCTCCTCGCCCAACACGCCTGGCCTGCGCAACATGCAGGCGCGCGAGCA
GCTCGGCGAACTTTTGTCGCGTGTCATGGCTGCGCGTGCGTCAGCCGCAACCCAGCCGCCCATCTTCCTCAAGATCGCGC
CGGATCTGGTCGAGGCCGAGCTGGAGGACATCGCCGCCGAGGTCGTTGAAAAGCGGATCGACGGCATCATCGTCTCCAAC
ACCACCATTTCGCGGCCACCGCTGCGCAGCGGCGACGTCGGACGTGAGACCGGCGGGCTTTCGGGAAAACCGCTGTTCGA
ACGCTCGACCATCGTGTTGGCGAAGATGCGCAAGCTGCTTGGCCCGGGCATAGCCATTATCGGGGTCGGCGGCGTCGACT
CCGCGGAGACCGCGCTGGAGAAAATCCGCGCCGGGGCCGATCTGGTGCAGCTCTACACCGGCATGATCTATGCCGGGCCT
GCGCTGCCGGGCCGTATCGTCGCCGGCATGGCACGCTTTTGCGAGACGGAGCGACTGAAGTCCATTGGCGAATTGCGCGA
CAGCCACCTCGATCGGTGGGCGTCCAAGCCACTCAGCTGA

Upstream 100 bases:

>100_bases
CGCATCTTTACGGGGTGCTGGACTTGAAAGCCGTGAAGTGGGTGCGGCCGCTGCCGCTCGGTGCCGATGGCGCACATCAG
TTCCCGCCGCTGGAGGGGCA

Downstream 100 bases:

>100_bases
ACGCCGTCCGCACCCGCAGCCGCAAAATCGTCAGCAGGCTGAAGCCGCGCGCCAGCAGGAAAACGTGCAGCGCCGCCCAC
AGGCCGTGATTGCCGAAGGC

Product: dihydroorotate dehydrogenase 2

Products: NA

Alternate protein names: DHOdehase; DHOD; DHODase; Dihydroorotate oxidase

Number of amino acids: Translated: 359; Mature: 358

Protein sequence:

>359_residues
MSVLDRLGQKLLFAFDPETAHGLSIAALRCGLPAGTRAVRDIRLKVSLCGLDFPNPLGMAAGYDKDAEVPDALLGLGFGF
AEVGTVTPLPQAGNPKPRIFRLTADEAVINRLGFNNEGHAAAEKRLAARKGRPGIVGVNIGANKDSADRIGDYERGVGRF
ARHASYLTVNISSPNTPGLRNMQAREQLGELLSRVMAARASAATQPPIFLKIAPDLVEAELEDIAAEVVEKRIDGIIVSN
TTISRPPLRSGDVGRETGGLSGKPLFERSTIVLAKMRKLLGPGIAIIGVGGVDSAETALEKIRAGADLVQLYTGMIYAGP
ALPGRIVAGMARFCETERLKSIGELRDSHLDRWASKPLS

Sequences:

>Translated_359_residues
MSVLDRLGQKLLFAFDPETAHGLSIAALRCGLPAGTRAVRDIRLKVSLCGLDFPNPLGMAAGYDKDAEVPDALLGLGFGF
AEVGTVTPLPQAGNPKPRIFRLTADEAVINRLGFNNEGHAAAEKRLAARKGRPGIVGVNIGANKDSADRIGDYERGVGRF
ARHASYLTVNISSPNTPGLRNMQAREQLGELLSRVMAARASAATQPPIFLKIAPDLVEAELEDIAAEVVEKRIDGIIVSN
TTISRPPLRSGDVGRETGGLSGKPLFERSTIVLAKMRKLLGPGIAIIGVGGVDSAETALEKIRAGADLVQLYTGMIYAGP
ALPGRIVAGMARFCETERLKSIGELRDSHLDRWASKPLS
>Mature_358_residues
SVLDRLGQKLLFAFDPETAHGLSIAALRCGLPAGTRAVRDIRLKVSLCGLDFPNPLGMAAGYDKDAEVPDALLGLGFGFA
EVGTVTPLPQAGNPKPRIFRLTADEAVINRLGFNNEGHAAAEKRLAARKGRPGIVGVNIGANKDSADRIGDYERGVGRFA
RHASYLTVNISSPNTPGLRNMQAREQLGELLSRVMAARASAATQPPIFLKIAPDLVEAELEDIAAEVVEKRIDGIIVSNT
TISRPPLRSGDVGRETGGLSGKPLFERSTIVLAKMRKLLGPGIAIIGVGGVDSAETALEKIRAGADLVQLYTGMIYAGPA
LPGRIVAGMARFCETERLKSIGELRDSHLDRWASKPLS

Specific function: Pyrimidine biosynthesis; fourth step. [C]

COG id: COG0167

COG function: function code F; Dihydroorotate dehydrogenase

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dihydroorotate dehydrogenase family. Type 2 subfamily

Homologues:

Organism=Homo sapiens, GI45006951, Length=321, Percent_Identity=47.9750778816199, Blast_Score=279, Evalue=2e-75,
Organism=Escherichia coli, GI1787177, Length=329, Percent_Identity=44.6808510638298, Blast_Score=245, Evalue=4e-66,
Organism=Caenorhabditis elegans, GI17509475, Length=343, Percent_Identity=46.064139941691, Blast_Score=266, Evalue=1e-71,
Organism=Drosophila melanogaster, GI281361352, Length=321, Percent_Identity=48.2866043613707, Blast_Score=283, Evalue=1e-76,
Organism=Drosophila melanogaster, GI17137316, Length=321, Percent_Identity=48.2866043613707, Blast_Score=283, Evalue=1e-76,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PYRD_RHILO (Q98B89)

Other databases:

- EMBL:   BA000012
- RefSeq:   NP_106297.1
- HSSP:   Q02127
- ProteinModelPortal:   Q98B89
- SMR:   Q98B89
- GeneID:   1228958
- GenomeReviews:   BA000012_GR
- KEGG:   mlo:mll5681
- NMPDR:   fig|266835.1.peg.4401
- HOGENOM:   HBG351027
- OMA:   AALNRMG
- ProtClustDB:   PRK05286
- HAMAP:   MF_00225
- InterPro:   IPR013785
- InterPro:   IPR012135
- InterPro:   IPR005719
- InterPro:   IPR001295
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF000164
- TIGRFAMs:   TIGR01036

Pfam domain/function: PF01180 DHO_dh

EC number: =1.3.5.2

Molecular weight: Translated: 38130; Mature: 37998

Theoretical pI: Translated: 9.64; Mature: 9.64

Prosite motif: PS00911 DHODEHASE_1; PS00912 DHODEHASE_2

Important sites: ACT_SITE 173-173

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVLDRLGQKLLFAFDPETAHGLSIAALRCGLPAGTRAVRDIRLKVSLCGLDFPNPLGMA
CCHHHHHHHHEEEEECCCCCCCCEEEHHHCCCCCCHHHHHHEEEEEEEECCCCCCCCCCC
AGYDKDAEVPDALLGLGFGFAEVGTVTPLPQAGNPKPRIFRLTADEAVINRLGFNNEGHA
CCCCCCCCCCHHHHHHCCCHHHHCCCCCCCCCCCCCCEEEEEEHHHHHHHHHCCCCCCHH
AAEKRLAARKGRPGIVGVNIGANKDSADRIGDYERGVGRFARHASYLTVNISSPNTPGLR
HHHHHHHHHCCCCCEEEEECCCCCCCHHHCCHHHHHHHHHHHCCEEEEEEECCCCCCCCC
NMQAREQLGELLSRVMAARASAATQPPIFLKIAPDLVEAELEDIAAEVVEKRIDGIIVSN
CHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHCCEEEEC
TTISRPPLRSGDVGRETGGLSGKPLFERSTIVLAKMRKLLGPGIAIIGVGGVDSAETALE
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHH
KIRAGADLVQLYTGMIYAGPALPGRIVAGMARFCETERLKSIGELRDSHLDRWASKPLS
HHHCCHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SVLDRLGQKLLFAFDPETAHGLSIAALRCGLPAGTRAVRDIRLKVSLCGLDFPNPLGMA
CHHHHHHHHEEEEECCCCCCCCEEEHHHCCCCCCHHHHHHEEEEEEEECCCCCCCCCCC
AGYDKDAEVPDALLGLGFGFAEVGTVTPLPQAGNPKPRIFRLTADEAVINRLGFNNEGHA
CCCCCCCCCCHHHHHHCCCHHHHCCCCCCCCCCCCCCEEEEEEHHHHHHHHHCCCCCCHH
AAEKRLAARKGRPGIVGVNIGANKDSADRIGDYERGVGRFARHASYLTVNISSPNTPGLR
HHHHHHHHHCCCCCEEEEECCCCCCCHHHCCHHHHHHHHHHHCCEEEEEEECCCCCCCCC
NMQAREQLGELLSRVMAARASAATQPPIFLKIAPDLVEAELEDIAAEVVEKRIDGIIVSN
CHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHCCEEEEC
TTISRPPLRSGDVGRETGGLSGKPLFERSTIVLAKMRKLLGPGIAIIGVGGVDSAETALE
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHH
KIRAGADLVQLYTGMIYAGPALPGRIVAGMARFCETERLKSIGELRDSHLDRWASKPLS
HHHCCHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11214968