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The map label for this gene is metZ [H]

Identifier: 13474705

GI number: 13474705

Start: 4542046

End: 4543236

Strand: Reverse

Name: metZ [H]

Synonym: mll5651

Alternate gene names: 13474705

Gene position: 4543236-4542046 (Counterclockwise)

Preceding gene: 13474708

Following gene: 13474703

Centisome position: 64.57

GC content: 64.06

Gene sequence:

>1191_bases
ATGAGCAACAAGAAGCGCAACTGGAAGCCTCAGACAGCACTCGTGCATAGCGGAACCCTGCGTTCAGGCTTCGGCGAGAC
CTCCGAGGCAATGTACCTGACCCAGGGCTATGTCTATCAAACGGCGCAAGCCGCCGAAGCCCGTTTCAAGGGCGAGGAAC
CCGGTTTCATCTATTCGCGCTACGCCAATCCGACCGTCGACATGTTCGAAAAGCGCATGTGCGCGCTGGAAGGCGCGGAG
GACGCGCGCGCCACGGCATCGGGCATGGCGGCCGTGACGGCGGCGCTTCTGTGCAGCGTCAAGACCGGTGACCACATCGT
AGCGGCGCGCGCGCTGTTCGGCTCCTGCCGCTGGGTGGTCGAGACGCTGGCGCCGCGCTACGGCATCGAGGCGACGCTCG
TCGACGGCACCGATATCGCCAACTGGGAAAAGGTGGTCAGGCCCAACACCAAGCTGTTCTTCCTGGAAAGCCCTACCAAC
CCGACATTGGAAGTGGTCGACATCGCCGCCGTGGCTGCGCTCGCCAACTCGATCGGCGCGCGGCTTGTCGTCGACAATGT
CTTCGCCACGCCCCTGCAGCAGAAGCCGTTGCAGCTCGGCGCGCATATCGTCGTCTATTCGGCAACCAAGCACATCGACG
GCCAGGGCCGCTGTCTCGGCGGCGTGATCCTGTCGGACAAGAAGTGGATCGACGAGAATCTGCATGACTATTTCCGCCAC
ACCGGCCCTAGCCTGTCGCCCTTCAACGCCTGGACGCTGCTGAAGGGCCTGGAGACGCTGCCGCTGCGCGTGCGCCAGCA
AACGGAGAGCGCCGGCAAGATCGCCGATTTCCTGGCCGAGCAGCCGCAGATCGCCCGCGTCATCTATCCCGGCCGCGCCG
ACCATCCGCAGGCCGAGATCGTCAAGAAGCAGATGTCGGGCGGCTCGACACTTATCTGCCTCGACGTCAAAGGCGGCAAG
CAGGCGGCCTTCGCCCTGCAGAACGCGCTCGATATTGTGCTGATCTCCAACAATCTCGGCGACGCCAAGAGCCTGATCAC
CCATCCGGCGACGACCACCCACAAGAATTTGAGCGACGAGGCGCGCGCCGAACTCGGCATCGGGCCGGGCACGCTCAGGC
TGTCGGTCGGCCTGGAGGATACCGACGACCTTCTGGAAGATATCGCGCAGGCGCTGAAGGCCGCCAAATAG

Upstream 100 bases:

>100_bases
GCAGCCACGTTAAACAAGTCGCTAAAGGACCGTTGGCCGCGAGGCCGTATGGACCGGTTGCGACTTTCGCGGCCGGTTTT
TTTGTGTCTGGAACAAAGCG

Downstream 100 bases:

>100_bases
GGAGCCGTCGAGGCAGCGGCGTCGGCGCCGCGATCAGGCGCGCGACGACGCCTCTTCCAGTTCGGCCGCCTTGGTGCCGA
TGGTCATCGCATTGCCGCGC

Product: O-succinylhomoserine sulfhydrylase

Products: Cystathionine; succinate; o-succinyl-L-homoserine; H2O [C]

Alternate protein names: OSH sulfhydrylase [H]

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MSNKKRNWKPQTALVHSGTLRSGFGETSEAMYLTQGYVYQTAQAAEARFKGEEPGFIYSRYANPTVDMFEKRMCALEGAE
DARATASGMAAVTAALLCSVKTGDHIVAARALFGSCRWVVETLAPRYGIEATLVDGTDIANWEKVVRPNTKLFFLESPTN
PTLEVVDIAAVAALANSIGARLVVDNVFATPLQQKPLQLGAHIVVYSATKHIDGQGRCLGGVILSDKKWIDENLHDYFRH
TGPSLSPFNAWTLLKGLETLPLRVRQQTESAGKIADFLAEQPQIARVIYPGRADHPQAEIVKKQMSGGSTLICLDVKGGK
QAAFALQNALDIVLISNNLGDAKSLITHPATTTHKNLSDEARAELGIGPGTLRLSVGLEDTDDLLEDIAQALKAAK

Sequences:

>Translated_396_residues
MSNKKRNWKPQTALVHSGTLRSGFGETSEAMYLTQGYVYQTAQAAEARFKGEEPGFIYSRYANPTVDMFEKRMCALEGAE
DARATASGMAAVTAALLCSVKTGDHIVAARALFGSCRWVVETLAPRYGIEATLVDGTDIANWEKVVRPNTKLFFLESPTN
PTLEVVDIAAVAALANSIGARLVVDNVFATPLQQKPLQLGAHIVVYSATKHIDGQGRCLGGVILSDKKWIDENLHDYFRH
TGPSLSPFNAWTLLKGLETLPLRVRQQTESAGKIADFLAEQPQIARVIYPGRADHPQAEIVKKQMSGGSTLICLDVKGGK
QAAFALQNALDIVLISNNLGDAKSLITHPATTTHKNLSDEARAELGIGPGTLRLSVGLEDTDDLLEDIAQALKAAK
>Mature_395_residues
SNKKRNWKPQTALVHSGTLRSGFGETSEAMYLTQGYVYQTAQAAEARFKGEEPGFIYSRYANPTVDMFEKRMCALEGAED
ARATASGMAAVTAALLCSVKTGDHIVAARALFGSCRWVVETLAPRYGIEATLVDGTDIANWEKVVRPNTKLFFLESPTNP
TLEVVDIAAVAALANSIGARLVVDNVFATPLQQKPLQLGAHIVVYSATKHIDGQGRCLGGVILSDKKWIDENLHDYFRHT
GPSLSPFNAWTLLKGLETLPLRVRQQTESAGKIADFLAEQPQIARVIYPGRADHPQAEIVKKQMSGGSTLICLDVKGGKQ
AAFALQNALDIVLISNNLGDAKSLITHPATTTHKNLSDEARAELGIGPGTLRLSVGLEDTDDLLEDIAQALKAAK

Specific function: Converts O-succinylhomoserine into homocysteine [H]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=350, Percent_Identity=38.5714285714286, Blast_Score=213, Evalue=3e-55,
Organism=Homo sapiens, GI299473759, Length=350, Percent_Identity=35.4285714285714, Blast_Score=182, Evalue=5e-46,
Organism=Homo sapiens, GI25453487, Length=349, Percent_Identity=32.9512893982808, Blast_Score=156, Evalue=2e-38,
Organism=Escherichia coli, GI1790375, Length=339, Percent_Identity=33.3333333333333, Blast_Score=201, Evalue=7e-53,
Organism=Escherichia coli, GI1789383, Length=396, Percent_Identity=28.030303030303, Blast_Score=140, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI17538055, Length=348, Percent_Identity=39.6551724137931, Blast_Score=239, Evalue=2e-63,
Organism=Caenorhabditis elegans, GI17560056, Length=354, Percent_Identity=39.5480225988701, Blast_Score=236, Evalue=2e-62,
Organism=Caenorhabditis elegans, GI71980708, Length=372, Percent_Identity=33.8709677419355, Blast_Score=205, Evalue=3e-53,
Organism=Saccharomyces cerevisiae, GI6319307, Length=360, Percent_Identity=39.7222222222222, Blast_Score=229, Evalue=8e-61,
Organism=Saccharomyces cerevisiae, GI6323333, Length=408, Percent_Identity=35.2941176470588, Blast_Score=191, Evalue=1e-49,
Organism=Saccharomyces cerevisiae, GI6321254, Length=364, Percent_Identity=28.021978021978, Blast_Score=127, Evalue=3e-30,
Organism=Saccharomyces cerevisiae, GI6321170, Length=272, Percent_Identity=29.0441176470588, Blast_Score=125, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6321904, Length=355, Percent_Identity=29.8591549295775, Blast_Score=116, Evalue=7e-27,
Organism=Drosophila melanogaster, GI19922554, Length=347, Percent_Identity=37.1757925072046, Blast_Score=230, Evalue=2e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006234
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: 2.5.1.48 [C]

Molecular weight: Translated: 42768; Mature: 42637

Theoretical pI: Translated: 7.01; Mature: 7.01

Prosite motif: PS00630 IMP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNKKRNWKPQTALVHSGTLRSGFGETSEAMYLTQGYVYQTAQAAEARFKGEEPGFIYSR
CCCCCCCCCCCEEEEECCCCCCCCCCCCCEEEEECCCEEHHHHHHHHHCCCCCCCEEEEE
YANPTVDMFEKRMCALEGAEDARATASGMAAVTAALLCSVKTGDHIVAARALFGSCRWVV
CCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
ETLAPRYGIEATLVDGTDIANWEKVVRPNTKLFFLESPTNPTLEVVDIAAVAALANSIGA
HHHCCCCCCEEEEECCCCCCCHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCC
RLVVDNVFATPLQQKPLQLGAHIVVYSATKHIDGQGRCLGGVILSDKKWIDENLHDYFRH
EEEEHHHHCCCCCCCCHHHCCEEEEEEECCCCCCCCCEEEEEEECCCHHHHHHHHHHHHH
TGPSLSPFNAWTLLKGLETLPLRVRQQTESAGKIADFLAEQPQIARVIYPGRADHPQAEI
CCCCCCCCHHHHHHHCHHHCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHH
VKKQMSGGSTLICLDVKGGKQAAFALQNALDIVLISNNLGDAKSLITHPATTTHKNLSDE
HHHHHCCCCEEEEEECCCCCHHHHHHHCCEEEEEEECCCCCHHHHHCCCCCCCCCCCCHH
ARAELGIGPGTLRLSVGLEDTDDLLEDIAQALKAAK
HHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SNKKRNWKPQTALVHSGTLRSGFGETSEAMYLTQGYVYQTAQAAEARFKGEEPGFIYSR
CCCCCCCCCCEEEEECCCCCCCCCCCCCEEEEECCCEEHHHHHHHHHCCCCCCCEEEEE
YANPTVDMFEKRMCALEGAEDARATASGMAAVTAALLCSVKTGDHIVAARALFGSCRWVV
CCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
ETLAPRYGIEATLVDGTDIANWEKVVRPNTKLFFLESPTNPTLEVVDIAAVAALANSIGA
HHHCCCCCCEEEEECCCCCCCHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCC
RLVVDNVFATPLQQKPLQLGAHIVVYSATKHIDGQGRCLGGVILSDKKWIDENLHDYFRH
EEEEHHHHCCCCCCCCHHHCCEEEEEEECCCCCCCCCEEEEEEECCCHHHHHHHHHHHHH
TGPSLSPFNAWTLLKGLETLPLRVRQQTESAGKIADFLAEQPQIARVIYPGRADHPQAEI
CCCCCCCCHHHHHHHCHHHCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHH
VKKQMSGGSTLICLDVKGGKQAAFALQNALDIVLISNNLGDAKSLITHPATTTHKNLSDE
HHHHHCCCCEEEEEECCCCCHHHHHHHCCEEEEEEECCCCCHHHHHCCCCCCCCCCCCHH
ARAELGIGPGTLRLSVGLEDTDDLLEDIAQALKAAK
HHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: pyridoxal 5'-phosphate [C]

Metal ions: NA

Kcat value (1/min): 460 [C]

Specific activity: 10

Km value (mM): 0.33 {O-succinyl-L-homoserine}} [C]

Substrates: O-succinyl-L-homoserine; @CYST01.txt*L-cysteine!; 2-oxobutanoate; succinate; NH3 [C]

Specific reaction: O-succinyl-L-homoserine + @CYST01.txt*L-cysteine! = cystathionine + succinate 2-oxobutanoate + succinate + NH3 = o-succinyl-L-homoserine + H2O [C]

General reaction: Elimination; Gamma-replacement [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7704274; 10984043 [H]