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The map label for this gene is pnp

Identifier: 13474634

GI number: 13474634

Start: 4462996

End: 4465143

Strand: Direct

Name: pnp

Synonym: mlr5562

Alternate gene names: 13474634

Gene position: 4462996-4465143 (Clockwise)

Preceding gene: 13474633

Following gene: 13474635

Centisome position: 63.43

GC content: 62.9

Gene sequence:

>2148_bases
ATGTTCAATCAGCACAAAGTGGAAATCGAATGGGGCGGTCGCCCGCTCATCCTCGAAACCGGCAAGATCGCCCGTCAGGC
TGACGGCGCTGTGCTCGCCACCTACGGCGAGACCAAGGTTCTGGCCACCGTCGTTTCGATGAAGGAGCCCAAGCCCGGCC
TCGATTTCTTTCCGCTGACCGTCAACTACCAGGAAAAGACCTATGCCGCCGGCAAGATCCCGGGCGGCTATTTCAAGCGC
GAAGGCCGTCCGAGCGAAAAGGAAACGCTGGTTTCGCGCCTCATCGACCGCCCCATCCGTCCGCTCTTCGCCGACGGCTA
CAAGAACGACACCCAGATCGTCGTCACCGTCGTTCAGCATGACCTCGAGAACGATCCGGACATCCTGTCGATCGTTGCCA
CCTCGGCCGCTTTGACCCTGTCGGGCGTTCCCTTCATGGGCCCGGTCGGCGGCGCGCGCGTCGGCTACATCAACGGCGAA
TACGTCCTCAACCCGCACATCGACGAAATGCAGGAATCCAAGCTCGACCTCGTCGTCGCCGGCACCGCCGACGCCGTGCT
GATGGTTGAGTCGGAAGCCAAGGAACTTGGCGAAGAACTGATGCTCGGCGCCGTCATGTTCGGCCACAAGGGCTTTCAGC
CGGTCATCGACGCCATCATCAAGCTGGCCGAAGTCGCCGCCAAGGAGCCACGCGACTTCACCGCGCCGGACTATTCCGCG
CTCGAAGGCGAGATGCTGAAGATCGTCGGCGACGAGCTCAGCAATGCCTACAAGATCACCGACAAGCAGCAACGCTATGC
CGCCGTCGACGCCGTCAAGGCGAAGGTCAAGGCAGCCTTTGCTCCGGCCGAAGGCGAAGAGCCCAAATACACCTCCGAGC
AGATCGGCTCGGTGTTCAAGGAACTCCAGGCCAAGGTCGTGCGCTGGAACATCCTCGACACCGGCTCGCGCATCGATGGC
CGCGACCTGAAGACGGTCCGCAAGATCGTTTCGGAAGTCGGCGTCCTGCCGCGCACCCATGGTTCGGCACTGTTCACCCG
CGGCGAAACCCAGGCGCTGGTCGTGGCCACGCTCGGCACCGGCGAAGACGAGCAGTATGTCGATTCGCTGACCGGCATGT
ACAAGGAGAAGTTCCTCCTTCACTACAACTTCCCTCCCTATTCCGTCGGTGAGACCGGCCGCATGGGTTCGCCGGGCCGC
CGCGAAATCGGCCACGGCAAGCTCGCCTGGCGCGCCATCCGGCCGATGCTGCCGAGCGCTGACCAGTTCCCCTACACGCT
GCGTGTCGTCTCGGAGATTACCGAGTCCAACGGCTCGTCCTCGATGGCCACCGTCTGCGGCACCTCCCTGGCGCTGATGG
ATGCCGGCGTGCCGCTGGCGAAGCCCGTTGCCGGTATCGCCATGGGCCTGATCAAGGAAGGCGAGCGCTTCGCCGTGCTC
TCCGACATCCTGGGCGACGAGGATCACCTCGGCGACATGGACTTCAAGGTCGCCGGCACCGCCAACGGCATCACTTCGCT
GCAGATGGACATCAAGATCGAGGGCATCACCGAGGAGATCATGAAGATCGCGCTGGACCAGGCCAAGGATGGCCGCCAGC
ATATCCTCGGCGAAATGGCGCATGCTCTGACCGGCGCACGCCCCGAACTCGGCGAATTCGCACCGCGCATCGAGGTCATG
CACATCCCGACCGACAAGATCCGCGATGTCATCGGTTCGGGCGGCAAGGTCATCCGCGAGATCGTCGAGAAGACCGGCGC
CAAGATCAACATCGAGGACGACGGCACGGTGAAGATCGCTTCCTCGAACGCCAAGGAGATCGAGGCGGCGAAGAAGTGGA
TCCACACCATTGTCGCCGAGCCGGAAGTCGGCGAGATCTACGAAGGCACGGTCGTCAAGACCGCCGACTTCGGCGCTTTC
GTCAACTTCTTCGGTCCGCGTGACGGCCTCGTCCACATCTCGCAGCTCGCCAATGAGCGCGTTGCCAAGACCTCGGACGT
TGTCAAGGAAGGCGACAAGGTTTGGGTCAAGCTGATGGGCTTCGACGAGCGCGGCAAGGTTCGCCTGTCGATGAAGGTCG
TCGACCAGGCCACCGGCAAGGAAATCGTGCGCGACAAGAGGGCCGAAGGCGAAGAAGACGCCGCCTGA

Upstream 100 bases:

>100_bases
CCCGTTGTCTTGCCCGTGGCTGCCCGAGAAAGGAAGCCAGAGGAAAGGGCACCCGCGCACGCTGAAACGGCGCGGCCCTT
CTTCATACAAAGGACAAGAC

Downstream 100 bases:

>100_bases
GCGCTCTGACAGAAAAAATCGAAGCGGGCGCGGCTTATGTCGCGCCCGTTTTCGTTTGGAGCAGTTCCAGGAAAGTATGA
GGCGCTTTCACCTTGGGAAT

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase

Number of amino acids: Translated: 715; Mature: 715

Protein sequence:

>715_residues
MFNQHKVEIEWGGRPLILETGKIARQADGAVLATYGETKVLATVVSMKEPKPGLDFFPLTVNYQEKTYAAGKIPGGYFKR
EGRPSEKETLVSRLIDRPIRPLFADGYKNDTQIVVTVVQHDLENDPDILSIVATSAALTLSGVPFMGPVGGARVGYINGE
YVLNPHIDEMQESKLDLVVAGTADAVLMVESEAKELGEELMLGAVMFGHKGFQPVIDAIIKLAEVAAKEPRDFTAPDYSA
LEGEMLKIVGDELSNAYKITDKQQRYAAVDAVKAKVKAAFAPAEGEEPKYTSEQIGSVFKELQAKVVRWNILDTGSRIDG
RDLKTVRKIVSEVGVLPRTHGSALFTRGETQALVVATLGTGEDEQYVDSLTGMYKEKFLLHYNFPPYSVGETGRMGSPGR
REIGHGKLAWRAIRPMLPSADQFPYTLRVVSEITESNGSSSMATVCGTSLALMDAGVPLAKPVAGIAMGLIKEGERFAVL
SDILGDEDHLGDMDFKVAGTANGITSLQMDIKIEGITEEIMKIALDQAKDGRQHILGEMAHALTGARPELGEFAPRIEVM
HIPTDKIRDVIGSGGKVIREIVEKTGAKINIEDDGTVKIASSNAKEIEAAKKWIHTIVAEPEVGEIYEGTVVKTADFGAF
VNFFGPRDGLVHISQLANERVAKTSDVVKEGDKVWVKLMGFDERGKVRLSMKVVDQATGKEIVRDKRAEGEEDAA

Sequences:

>Translated_715_residues
MFNQHKVEIEWGGRPLILETGKIARQADGAVLATYGETKVLATVVSMKEPKPGLDFFPLTVNYQEKTYAAGKIPGGYFKR
EGRPSEKETLVSRLIDRPIRPLFADGYKNDTQIVVTVVQHDLENDPDILSIVATSAALTLSGVPFMGPVGGARVGYINGE
YVLNPHIDEMQESKLDLVVAGTADAVLMVESEAKELGEELMLGAVMFGHKGFQPVIDAIIKLAEVAAKEPRDFTAPDYSA
LEGEMLKIVGDELSNAYKITDKQQRYAAVDAVKAKVKAAFAPAEGEEPKYTSEQIGSVFKELQAKVVRWNILDTGSRIDG
RDLKTVRKIVSEVGVLPRTHGSALFTRGETQALVVATLGTGEDEQYVDSLTGMYKEKFLLHYNFPPYSVGETGRMGSPGR
REIGHGKLAWRAIRPMLPSADQFPYTLRVVSEITESNGSSSMATVCGTSLALMDAGVPLAKPVAGIAMGLIKEGERFAVL
SDILGDEDHLGDMDFKVAGTANGITSLQMDIKIEGITEEIMKIALDQAKDGRQHILGEMAHALTGARPELGEFAPRIEVM
HIPTDKIRDVIGSGGKVIREIVEKTGAKINIEDDGTVKIASSNAKEIEAAKKWIHTIVAEPEVGEIYEGTVVKTADFGAF
VNFFGPRDGLVHISQLANERVAKTSDVVKEGDKVWVKLMGFDERGKVRLSMKVVDQATGKEIVRDKRAEGEEDAA
>Mature_715_residues
MFNQHKVEIEWGGRPLILETGKIARQADGAVLATYGETKVLATVVSMKEPKPGLDFFPLTVNYQEKTYAAGKIPGGYFKR
EGRPSEKETLVSRLIDRPIRPLFADGYKNDTQIVVTVVQHDLENDPDILSIVATSAALTLSGVPFMGPVGGARVGYINGE
YVLNPHIDEMQESKLDLVVAGTADAVLMVESEAKELGEELMLGAVMFGHKGFQPVIDAIIKLAEVAAKEPRDFTAPDYSA
LEGEMLKIVGDELSNAYKITDKQQRYAAVDAVKAKVKAAFAPAEGEEPKYTSEQIGSVFKELQAKVVRWNILDTGSRIDG
RDLKTVRKIVSEVGVLPRTHGSALFTRGETQALVVATLGTGEDEQYVDSLTGMYKEKFLLHYNFPPYSVGETGRMGSPGR
REIGHGKLAWRAIRPMLPSADQFPYTLRVVSEITESNGSSSMATVCGTSLALMDAGVPLAKPVAGIAMGLIKEGERFAVL
SDILGDEDHLGDMDFKVAGTANGITSLQMDIKIEGITEEIMKIALDQAKDGRQHILGEMAHALTGARPELGEFAPRIEVM
HIPTDKIRDVIGSGGKVIREIVEKTGAKINIEDDGTVKIASSNAKEIEAAKKWIHTIVAEPEVGEIYEGTVVKTADFGAF
VNFFGPRDGLVHISQLANERVAKTSDVVKEGDKVWVKLMGFDERGKVRLSMKVVDQATGKEIVRDKRAEGEEDAA

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain

Homologues:

Organism=Homo sapiens, GI188528628, Length=727, Percent_Identity=37.0013755158184, Blast_Score=442, Evalue=1e-124,
Organism=Homo sapiens, GI21361576, Length=86, Percent_Identity=47.6744186046512, Blast_Score=76, Evalue=1e-13,
Organism=Homo sapiens, GI4826690, Length=92, Percent_Identity=46.7391304347826, Blast_Score=73, Evalue=1e-12,
Organism=Escherichia coli, GI145693187, Length=693, Percent_Identity=56.998556998557, Blast_Score=787, Evalue=0.0,
Organism=Caenorhabditis elegans, GI115534063, Length=713, Percent_Identity=32.8190743338008, Blast_Score=342, Evalue=3e-94,
Organism=Caenorhabditis elegans, GI17535281, Length=84, Percent_Identity=48.8095238095238, Blast_Score=75, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6320850, Length=122, Percent_Identity=35.2459016393443, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI281362905, Length=722, Percent_Identity=36.8421052631579, Blast_Score=439, Evalue=1e-123,
Organism=Drosophila melanogaster, GI24651641, Length=722, Percent_Identity=36.8421052631579, Blast_Score=439, Evalue=1e-123,
Organism=Drosophila melanogaster, GI24651643, Length=722, Percent_Identity=36.8421052631579, Blast_Score=439, Evalue=1e-123,
Organism=Drosophila melanogaster, GI161079377, Length=666, Percent_Identity=36.6366366366366, Blast_Score=397, Evalue=1e-110,
Organism=Drosophila melanogaster, GI20129977, Length=87, Percent_Identity=47.1264367816092, Blast_Score=69, Evalue=1e-11,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): PNP_RHILO (Q98BI3)

Other databases:

- EMBL:   BA000012
- RefSeq:   NP_106203.1
- HSSP:   P05055
- ProteinModelPortal:   Q98BI3
- SMR:   Q98BI3
- GeneID:   1228864
- GenomeReviews:   BA000012_GR
- KEGG:   mlo:mlr5562
- NMPDR:   fig|266835.1.peg.4307
- HOGENOM:   HBG382411
- OMA:   YGETVVL
- ProtClustDB:   PRK11824
- GO:   GO:0005739
- HAMAP:   MF_01595
- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:1.10.10.400
- PANTHER:   PTHR11252
- PIRSF:   PIRSF005499
- SMART:   SM00322
- SMART:   SM00316
- TIGRFAMs:   TIGR03591

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1; SSF46915 3_ExoRNase; SSF55666 3_ExoRNase; SSF50249 Nucleic_acid_OB; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.7.8

Molecular weight: Translated: 77698; Mature: 77698

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: PS50084 KH_TYPE_1; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFNQHKVEIEWGGRPLILETGKIARQADGAVLATYGETKVLATVVSMKEPKPGLDFFPLT
CCCCEEEEEEECCEEEEEECCCHHHHCCCCEEEECCCHHHHHHHHHCCCCCCCCCEEEEE
VNYQEKTYAAGKIPGGYFKREGRPSEKETLVSRLIDRPIRPLFADGYKNDTQIVVTVVQH
EECCCCEEECCCCCCCHHCCCCCCCHHHHHHHHHHHCCCCHHHCCCCCCCCEEEEEEEHH
DLENDPDILSIVATSAALTLSGVPFMGPVGGARVGYINGEYVLNPHIDEMQESKLDLVVA
CCCCCCCHHHHHHHHHHHEECCCCCCCCCCCCEEEEECCCEEECCCHHHHHHCCCCEEEE
GTADAVLMVESEAKELGEELMLGAVMFGHKGFQPVIDAIIKLAEVAAKEPRDFTAPDYSA
CCCCEEEEEEHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHH
LEGEMLKIVGDELSNAYKITDKQQRYAAVDAVKAKVKAAFAPAEGEEPKYTSEQIGSVFK
HCCHHHHHHHHHHCCEEECCCHHHHHHHHHHHHHHHHHEECCCCCCCCCCCHHHHHHHHH
ELQAKVVRWNILDTGSRIDGRDLKTVRKIVSEVGVLPRTHGSALFTRGETQALVVATLGT
HHHHHHEEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEECCCCCEEEEEEECC
GEDEQYVDSLTGMYKEKFLLHYNFPPYSVGETGRMGSPGRREIGHGKLAWRAIRPMLPSA
CCCHHHHHHHHHHHHHEEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCC
DQFPYTLRVVSEITESNGSSSMATVCGTSLALMDAGVPLAKPVAGIAMGLIKEGERFAVL
CCCCHHHHHHHHHHHCCCCCCHHHHHCCHHHHHHCCCCHHHHHHHHHHHHHHCCCCEEHH
SDILGDEDHLGDMDFKVAGTANGITSLQMDIKIEGITEEIMKIALDQAKDGRQHILGEMA
HHHCCCCCCCCCCCEEEEECCCCCEEEEEEEEECCHHHHHHHHHHHHCCCCHHHHHHHHH
HALTGARPELGEFAPRIEVMHIPTDKIRDVIGSGGKVIREIVEKTGAKINIEDDGTVKIA
HHHCCCCCCHHHCCCCEEEEECCHHHHHHHHCCCHHHHHHHHHHCCCEEEECCCCEEEEE
SSNAKEIEAAKKWIHTIVAEPEVGEIYEGTVVKTADFGAFVNFFGPRDGLVHISQLANER
CCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCHHHHHCCCCCCHHHHHHHHHHH
VAKTSDVVKEGDKVWVKLMGFDERGKVRLSMKVVDQATGKEIVRDKRAEGEEDAA
HHHHHHHHHCCCEEEEEEECCCCCCCEEEEEEEHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MFNQHKVEIEWGGRPLILETGKIARQADGAVLATYGETKVLATVVSMKEPKPGLDFFPLT
CCCCEEEEEEECCEEEEEECCCHHHHCCCCEEEECCCHHHHHHHHHCCCCCCCCCEEEEE
VNYQEKTYAAGKIPGGYFKREGRPSEKETLVSRLIDRPIRPLFADGYKNDTQIVVTVVQH
EECCCCEEECCCCCCCHHCCCCCCCHHHHHHHHHHHCCCCHHHCCCCCCCCEEEEEEEHH
DLENDPDILSIVATSAALTLSGVPFMGPVGGARVGYINGEYVLNPHIDEMQESKLDLVVA
CCCCCCCHHHHHHHHHHHEECCCCCCCCCCCCEEEEECCCEEECCCHHHHHHCCCCEEEE
GTADAVLMVESEAKELGEELMLGAVMFGHKGFQPVIDAIIKLAEVAAKEPRDFTAPDYSA
CCCCEEEEEEHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHH
LEGEMLKIVGDELSNAYKITDKQQRYAAVDAVKAKVKAAFAPAEGEEPKYTSEQIGSVFK
HCCHHHHHHHHHHCCEEECCCHHHHHHHHHHHHHHHHHEECCCCCCCCCCCHHHHHHHHH
ELQAKVVRWNILDTGSRIDGRDLKTVRKIVSEVGVLPRTHGSALFTRGETQALVVATLGT
HHHHHHEEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEECCCCCEEEEEEECC
GEDEQYVDSLTGMYKEKFLLHYNFPPYSVGETGRMGSPGRREIGHGKLAWRAIRPMLPSA
CCCHHHHHHHHHHHHHEEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCC
DQFPYTLRVVSEITESNGSSSMATVCGTSLALMDAGVPLAKPVAGIAMGLIKEGERFAVL
CCCCHHHHHHHHHHHCCCCCCHHHHHCCHHHHHHCCCCHHHHHHHHHHHHHHCCCCEEHH
SDILGDEDHLGDMDFKVAGTANGITSLQMDIKIEGITEEIMKIALDQAKDGRQHILGEMA
HHHCCCCCCCCCCCEEEEECCCCCEEEEEEEEECCHHHHHHHHHHHHCCCCHHHHHHHHH
HALTGARPELGEFAPRIEVMHIPTDKIRDVIGSGGKVIREIVEKTGAKINIEDDGTVKIA
HHHCCCCCCHHHCCCCEEEEECCHHHHHHHHCCCHHHHHHHHHHCCCEEEECCCCEEEEE
SSNAKEIEAAKKWIHTIVAEPEVGEIYEGTVVKTADFGAFVNFFGPRDGLVHISQLANER
CCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCHHHHHCCCCCCHHHHHHHHHHH
VAKTSDVVKEGDKVWVKLMGFDERGKVRLSMKVVDQATGKEIVRDKRAEGEEDAA
HHHHHHHHHCCCEEEEEEECCCCCCCEEEEEEEHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11214968