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The map label for this gene is gph [H]

Identifier: 13474513

GI number: 13474513

Start: 4329042

End: 4329773

Strand: Direct

Name: gph [H]

Synonym: mlr5413

Alternate gene names: 13474513

Gene position: 4329042-4329773 (Clockwise)

Preceding gene: 13474512

Following gene: 13474516

Centisome position: 61.53

GC content: 64.89

Gene sequence:

>732_bases
GTGACTGATTTGGCAGACATCAAGGGGATATTGTTCGACAAGGACGGGACACTTGTCGACTTCAACGCGACCTGGCTCGG
CGTCGCCGATTTCATGGCCATGGACGCCTCCGACGGGGACCGCTGGAAGGCCGACAGGCTGCTTGCGGCCGCCGGCTTCG
ATTTCGCCAGCCGCCGCTTCAAGCCCGATTCGATCTTTGCCTCGGGCACCAATATGGATGTGGTCGAGCTGTGGTTCCCG
CGTCTGTCCGACGAAGACCAGATGCTGGCCGTCGCCCGCTTCAACGAAATCACCTCGGTGCAGGGCTCGGCGATGGCGGT
GGCGCTGCCGGGCGTGGTCGACACGCTGGCGGTGCTGCACAAAAGATCCTACCGGCTTGGGGTCGCCACCAACGATTCGA
CCAGCGGCGCGGAAAAGACCTTGGTCACGCTGGGCGTCGCCCAGCTGTTCGACGCGGCCTATGGTTATGACGCGGTGGCC
AATCCCAAACCCGCGCCCGACACCATCCAGGCCTTTTGCGACCTTACCGGCCTGAAGCCGGCTGAGGTCGCCATGGTCGG
TGACAACCGCCACGATCTCGAAATGGCGCGCGCCGGCGGCTGCGGCCTGGCGGTGGGCGTGCTCTCCGGCACCGGCACGC
GTGAGTCGCTGGCTGGCATTGCCGACGTCGTTCTCGATTCCGTTGCCGATCTGCCGGACTTCCTGTCGGCCCGGGTCAAG
GAGACGGTTTGA

Upstream 100 bases:

>100_bases
CCGGCCTGCTGCCTGTCGTGGAAGGCAAGGAAAGCGCCGCAAACCGGGACGCCTCGACCAGGGGCCTTGTGGAACGCATC
CACCAATTGCGTGGTTCGGA

Downstream 100 bases:

>100_bases
TCGCTCCCGCCAGCGCTGGTGCGGTCTCGGCCTCGAAACGAAAGACGCCGCTCGGCTTCAGCGCGGCGTCTTTCGCGTCA
CCGAAATCCGGCACCTGATA

Product: phosphoglycolate phosphatase

Products: NA

Alternate protein names: PGP; PGPase [H]

Number of amino acids: Translated: 243; Mature: 242

Protein sequence:

>243_residues
MTDLADIKGILFDKDGTLVDFNATWLGVADFMAMDASDGDRWKADRLLAAAGFDFASRRFKPDSIFASGTNMDVVELWFP
RLSDEDQMLAVARFNEITSVQGSAMAVALPGVVDTLAVLHKRSYRLGVATNDSTSGAEKTLVTLGVAQLFDAAYGYDAVA
NPKPAPDTIQAFCDLTGLKPAEVAMVGDNRHDLEMARAGGCGLAVGVLSGTGTRESLAGIADVVLDSVADLPDFLSARVK
ETV

Sequences:

>Translated_243_residues
MTDLADIKGILFDKDGTLVDFNATWLGVADFMAMDASDGDRWKADRLLAAAGFDFASRRFKPDSIFASGTNMDVVELWFP
RLSDEDQMLAVARFNEITSVQGSAMAVALPGVVDTLAVLHKRSYRLGVATNDSTSGAEKTLVTLGVAQLFDAAYGYDAVA
NPKPAPDTIQAFCDLTGLKPAEVAMVGDNRHDLEMARAGGCGLAVGVLSGTGTRESLAGIADVVLDSVADLPDFLSARVK
ETV
>Mature_242_residues
TDLADIKGILFDKDGTLVDFNATWLGVADFMAMDASDGDRWKADRLLAAAGFDFASRRFKPDSIFASGTNMDVVELWFPR
LSDEDQMLAVARFNEITSVQGSAMAVALPGVVDTLAVLHKRSYRLGVATNDSTSGAEKTLVTLGVAQLFDAAYGYDAVAN
PKPAPDTIQAFCDLTGLKPAEVAMVGDNRHDLEMARAGGCGLAVGVLSGTGTRESLAGIADVVLDSVADLPDFLSARVKE
TV

Specific function: Specifically catalyzes the dephosphorylation of 2- phosphoglycolate. Is involved in the dissimilation of the intracellular 2-phosphoglycolate formed during the DNA repair of 3'-phosphoglycolate ends, a major class of DNA lesions induced by oxidative stres

COG id: COG0546

COG function: function code R; Predicted phosphatases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family [H]

Homologues:

Organism=Escherichia coli, GI1789787, Length=239, Percent_Identity=29.7071129707113, Blast_Score=79, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI71983424, Length=254, Percent_Identity=27.5590551181102, Blast_Score=76, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17567717, Length=196, Percent_Identity=29.0816326530612, Blast_Score=64, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439
- InterPro:   IPR006402
- InterPro:   IPR005833
- InterPro:   IPR006346
- InterPro:   IPR023198 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: =3.1.3.18 [H]

Molecular weight: Translated: 25714; Mature: 25583

Theoretical pI: Translated: 4.24; Mature: 4.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDLADIKGILFDKDGTLVDFNATWLGVADFMAMDASDGDRWKADRLLAAAGFDFASRRF
CCCHHHCCEEEECCCCCEEEECCHHHHHHHHHEECCCCCCCHHHHHHHHHHCCHHHHCCC
KPDSIFASGTNMDVVELWFPRLSDEDQMLAVARFNEITSVQGSAMAVALPGVVDTLAVLH
CCCCEEECCCCCCCCEEECCCCCCCCCEEHHHHHHHHHCCCCCEEEEECCHHHHHHHHHH
KRSYRLGVATNDSTSGAEKTLVTLGVAQLFDAAYGYDAVANPKPAPDTIQAFCDLTGLKP
HCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCC
AEVAMVGDNRHDLEMARAGGCGLAVGVLSGTGTRESLAGIADVVLDSVADLPDFLSARVK
CEEEEECCCCHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHH
ETV
HCC
>Mature Secondary Structure 
TDLADIKGILFDKDGTLVDFNATWLGVADFMAMDASDGDRWKADRLLAAAGFDFASRRF
CCHHHCCEEEECCCCCEEEECCHHHHHHHHHEECCCCCCCHHHHHHHHHHCCHHHHCCC
KPDSIFASGTNMDVVELWFPRLSDEDQMLAVARFNEITSVQGSAMAVALPGVVDTLAVLH
CCCCEEECCCCCCCCEEECCCCCCCCCEEHHHHHHHHHCCCCCEEEEECCHHHHHHHHHH
KRSYRLGVATNDSTSGAEKTLVTLGVAQLFDAAYGYDAVANPKPAPDTIQAFCDLTGLKP
HCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCC
AEVAMVGDNRHDLEMARAGGCGLAVGVLSGTGTRESLAGIADVVLDSVADLPDFLSARVK
CEEEEECCCCHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHH
ETV
HCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]