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The map label for this gene is polS [H]

Identifier: 13474342

GI number: 13474342

Start: 4139586

End: 4140293

Strand: Direct

Name: polS [H]

Synonym: mlr5208

Alternate gene names: 13474342

Gene position: 4139586-4140293 (Clockwise)

Preceding gene: 13474341

Following gene: 13474347

Centisome position: 58.83

GC content: 65.96

Gene sequence:

>708_bases
ATGACGGAAACACTTCAAGGCAGGCATATCGTTGTGACCGGTGGGACCGGCGCTCTCGGCGGTGCGGTGGTTGGTCGGCT
GCTGGAACAGGGCGCCATCTGCCACGTGCCGAACGCACACGCCTCGGCACCCCAGCAATTTCCTTTCACCGCCCACGACA
ACGTCAAGCTGGCGCACAATGTCGACCTGTCGGACTCCGCCAAGGTCGAAGCCTTCTATGCCCAGGTTCCTGCCTTGTGG
GGATCGATCCATCTCGCCGGCGGCTTCACCATGGCGCCGGTGGAAAAGATCGAATCGGCATCCTTTGCCGAGATGATGGA
CACCAACGCCCGCACCACCTTCCTGTGCAGCCGCGCTGCGATCCGCTCGATGCTGGCGTTGGGCACATCAGGCCGCATCG
TCAATGTCACCGCGCGTGCCGGGCTCGACCCCAGGCGCGGCGCCGGCATGGTCGCCTACACGGCCAGCAAGGCGGCGGTG
GCGGCCATGACGGTGGCGATGGCCGAAGAGCTCAAGGCCAAGGGCATCCTGGTCAATGCCGTGGCGCCGTCGACGCTCGA
CACGCCGGCAAACCGCGCCGACATGCCGGATGCCGATTTCAGCAAATGGGTCAGCCTCGAAGCGGCGGCGGAAGCCATCG
CCTATCTCGCCTCGCCGGCCAACCAGGCGATGAGCGGAACGCTGGTGCCGCTCTACGGCCGGGCCTGA

Upstream 100 bases:

>100_bases
CGAGATATCAATTGAGTGATCGCCCGAAAGGCGCGACGCTCCGCGTCGGGGCGGCACAACAAGCGACACAATCATAAAAA
CACGACAGGAGCGGTCCAGG

Downstream 100 bases:

>100_bases
GCGGCGAGCGTCGAAGTCGGGCAGACGGCATCCGATCCATCGGCATTGCGATAGCTGTAGATCCGCAGCGGTGATGAAGC
TTTCGTGGGGCACTCCCGCA

Product: short-chain dehydrogenase/reductase family protein

Products: NA

Alternate protein names: L-iditol 2-dehydrogenase; Polyol dehydrogenase [H]

Number of amino acids: Translated: 235; Mature: 234

Protein sequence:

>235_residues
MTETLQGRHIVVTGGTGALGGAVVGRLLEQGAICHVPNAHASAPQQFPFTAHDNVKLAHNVDLSDSAKVEAFYAQVPALW
GSIHLAGGFTMAPVEKIESASFAEMMDTNARTTFLCSRAAIRSMLALGTSGRIVNVTARAGLDPRRGAGMVAYTASKAAV
AAMTVAMAEELKAKGILVNAVAPSTLDTPANRADMPDADFSKWVSLEAAAEAIAYLASPANQAMSGTLVPLYGRA

Sequences:

>Translated_235_residues
MTETLQGRHIVVTGGTGALGGAVVGRLLEQGAICHVPNAHASAPQQFPFTAHDNVKLAHNVDLSDSAKVEAFYAQVPALW
GSIHLAGGFTMAPVEKIESASFAEMMDTNARTTFLCSRAAIRSMLALGTSGRIVNVTARAGLDPRRGAGMVAYTASKAAV
AAMTVAMAEELKAKGILVNAVAPSTLDTPANRADMPDADFSKWVSLEAAAEAIAYLASPANQAMSGTLVPLYGRA
>Mature_234_residues
TETLQGRHIVVTGGTGALGGAVVGRLLEQGAICHVPNAHASAPQQFPFTAHDNVKLAHNVDLSDSAKVEAFYAQVPALWG
SIHLAGGFTMAPVEKIESASFAEMMDTNARTTFLCSRAAIRSMLALGTSGRIVNVTARAGLDPRRGAGMVAYTASKAAVA
AMTVAMAEELKAKGILVNAVAPSTLDTPANRADMPDADFSKWVSLEAAAEAIAYLASPANQAMSGTLVPLYGRA

Specific function: Catalyzes the oxidation of D-glucitol (sorbitol) to D- fructose, galactitol to D-tagatose and of L-iditol [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Escherichia coli, GI1788459, Length=135, Percent_Identity=38.5185185185185, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI2367175, Length=245, Percent_Identity=28.9795918367347, Blast_Score=70, Evalue=8e-14,
Organism=Escherichia coli, GI1787820, Length=179, Percent_Identity=26.2569832402235, Blast_Score=63, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17555706, Length=248, Percent_Identity=28.2258064516129, Blast_Score=70, Evalue=8e-13,
Organism=Drosophila melanogaster, GI24643142, Length=245, Percent_Identity=28.1632653061224, Blast_Score=71, Evalue=6e-13,
Organism=Drosophila melanogaster, GI28571526, Length=255, Percent_Identity=27.0588235294118, Blast_Score=70, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: =1.1.1.14 [H]

Molecular weight: Translated: 24323; Mature: 24192

Theoretical pI: Translated: 7.14; Mature: 7.14

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTETLQGRHIVVTGGTGALGGAVVGRLLEQGAICHVPNAHASAPQQFPFTAHDNVKLAHN
CCCCCCCCEEEEECCCCCHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCCCEEEEEE
VDLSDSAKVEAFYAQVPALWGSIHLAGGFTMAPVEKIESASFAEMMDTNARTTFLCSRAA
CCCCCCCHHHHHHHHHHHHHCEEEECCCCEECHHHHHCCCHHHHHHCCCCCHHHHHHHHH
IRSMLALGTSGRIVNVTARAGLDPRRGAGMVAYTASKAAVAAMTVAMAEELKAKGILVNA
HHHHHHCCCCCCEEEEEECCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHCCEEEEE
VAPSTLDTPANRADMPDADFSKWVSLEAAAEAIAYLASPANQAMSGTLVPLYGRA
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHCCCEEEECCCC
>Mature Secondary Structure 
TETLQGRHIVVTGGTGALGGAVVGRLLEQGAICHVPNAHASAPQQFPFTAHDNVKLAHN
CCCCCCCEEEEECCCCCHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCCCEEEEEE
VDLSDSAKVEAFYAQVPALWGSIHLAGGFTMAPVEKIESASFAEMMDTNARTTFLCSRAA
CCCCCCCHHHHHHHHHHHHHCEEEECCCCEECHHHHHCCCHHHHHHCCCCCHHHHHHHHH
IRSMLALGTSGRIVNVTARAGLDPRRGAGMVAYTASKAAVAAMTVAMAEELKAKGILVNA
HHHHHHCCCCCCEEEEEECCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHCCEEEEE
VAPSTLDTPANRADMPDADFSKWVSLEAAAEAIAYLASPANQAMSGTLVPLYGRA
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHCCCEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7551049 [H]