Click here to switch to the map view.

The map label for this gene is ilvI [H]

Identifier: 13474341

GI number: 13474341

Start: 4137766

End: 4139346

Strand: Direct

Name: ilvI [H]

Synonym: mlr5207

Alternate gene names: 13474341

Gene position: 4137766-4139346 (Clockwise)

Preceding gene: 13474340

Following gene: 13474342

Centisome position: 58.81

GC content: 68.18

Gene sequence:

>1581_bases
ATGACAACACTCGGCGAGGCCCTGATCACACTGCTCGAAGCGCATGGCGTCGATACCGTCTTCGGCATTCCGGGCGTCCA
CACGGTCGAGCTCTACCGCGGCCTGGCGCGCTCGAAAATCCGTCATGTCACGCCGCGCCATGAACAGGGCGCCGGCTTCA
TGGCCGACGGCTATGCCCGCGCCAGCGGCAGGCCAGGCGTCGCCTTCGTCATCACCGGACCGGGCCTGACCAACACCATC
ACCGCCATGGGCCAGGCGCGCGCCGATTCGGTGCCGATGCTGGTCATATCGGGCGTCAACGCCACGCCGACGCTGGGCAA
GGGGCTGGGCTTCCTGCATGAGCTGCCGGACCAGCGCGGCATGATGGAAAAGGTGGCGCTGTTGTCGCGGCGCGTCACCG
AGGCAAGCGAATTGCCTGACGCGCTGGCACAGGCCTTCGCGCTGTTTTCATCCTCGCGGCCGGGCCCGGTGCATATCGAG
ATCCCGACCGATGTCATGGTCAAGCCGGCCGACGATATCGCGGCACTGTTGAGCAACGCGGCACCGCCGACGCCGGATGG
CGCGGCCATTGCCAGCGCAGCCAAGCTTATCACCGCCGCGCGCCGTCCGCTGATCCTGGCCGGAGGCGGCGCCAAGCGGG
CCGAGATGGCGCTGCGGCGCCTGGCCGAGACACTGGGCGCGCCGGTCGTCGAAACCACCAATGCGCGCGGCCTGCTGCAT
CGCCACCCGCTCTGCGTTCCGGCAAGCCCAAGCCTGAAGGCGGTTCGGGCGCTGATGGCCGAGGCCGATCTGGTGATTGC
CGCCGGCACCGAATTCGGCCCGACCGACTATGACGGCTATGGCGACGGTGGCTTTGTGCTGCCTTCCAATCTGATTCGCA
TCGACATCGGCGCTGACCAGCTCGCGCGCCGTCCGGTGACGGTCGGCATCCAGGCCGATTGCGCCGAGGCGATCGAAGCC
TTGCTCGCCGCGATCGGCACCGCTCATCCTGCCGCACCAGATGGAGAGGCGCGCGCCACCGCCGCGCGGAAGGCGGCGTT
CGCCGAACTGAGCCCGGCCTATCTGGCACAGGTGCATGCCGTGGAAATGATCCGCGATGCCTTGCCGGGCGCCATCGTCG
TCGGCGATTCGACGCAGCCGGTCTACGCCGCCAACCTCTACTATGATCACGACCGCCCGGGCGGCTGGTTCAACGCCGCC
ACCGGCTTCGGCGCGCTTGGTTACGGCCCGCCGGCGGCGATCGGCGCCGCACTTGCCGTGCCCGACGCGCCGGTTGTCTG
CTTGACCGGCGATGGCGGCTTCCAGTTCACCTTGCCTGAACTTGGCGCGGCGCTCGATGCAAAGGCGCCGGTCATCTTCG
TGGTCTGGAACAACAGGGGCTATCGTGAGATCGAGACCTCGATGCTCGATGTCGGCGTCGAGCCCGTCGGGGTCTCGCCG
GCGCCGCCGGATTTCTGCAAGCTGGCCGAAGCCTATGGCATCGGCGCCGAACGGCTCGCCGATATCGGCGCTCTGCCGCA
GGCCCTGAAGCGCGCCCGGGCAACCGGACTGCCTTGCGTCATCGAAATCACCGTCGATTGA

Upstream 100 bases:

>100_bases
GCCTGGCCGGCGAGATGTTCTCGCCCGGCGAATTGGCGGCGATTGGCATCAAGACCGTCGAGGCCGAACTGGGCCTCGCG
CCGGAAAGGGAAGACAATCA

Downstream 100 bases:

>100_bases
AGGCTTCAGCTCGGCGTTGATTTTCTCGTCTGCATCGGATCCTTCGTCGGCTCGGCTGTTGTACCGCAACGGCCGCATGG
CTGACGCCATTGCCATAATA

Product: hypothetical protein

Products: NA

Alternate protein names: AHAS-III; ALS-III; Acetohydroxy-acid synthase III large subunit [H]

Number of amino acids: Translated: 526; Mature: 525

Protein sequence:

>526_residues
MTTLGEALITLLEAHGVDTVFGIPGVHTVELYRGLARSKIRHVTPRHEQGAGFMADGYARASGRPGVAFVITGPGLTNTI
TAMGQARADSVPMLVISGVNATPTLGKGLGFLHELPDQRGMMEKVALLSRRVTEASELPDALAQAFALFSSSRPGPVHIE
IPTDVMVKPADDIAALLSNAAPPTPDGAAIASAAKLITAARRPLILAGGGAKRAEMALRRLAETLGAPVVETTNARGLLH
RHPLCVPASPSLKAVRALMAEADLVIAAGTEFGPTDYDGYGDGGFVLPSNLIRIDIGADQLARRPVTVGIQADCAEAIEA
LLAAIGTAHPAAPDGEARATAARKAAFAELSPAYLAQVHAVEMIRDALPGAIVVGDSTQPVYAANLYYDHDRPGGWFNAA
TGFGALGYGPPAAIGAALAVPDAPVVCLTGDGGFQFTLPELGAALDAKAPVIFVVWNNRGYREIETSMLDVGVEPVGVSP
APPDFCKLAEAYGIGAERLADIGALPQALKRARATGLPCVIEITVD

Sequences:

>Translated_526_residues
MTTLGEALITLLEAHGVDTVFGIPGVHTVELYRGLARSKIRHVTPRHEQGAGFMADGYARASGRPGVAFVITGPGLTNTI
TAMGQARADSVPMLVISGVNATPTLGKGLGFLHELPDQRGMMEKVALLSRRVTEASELPDALAQAFALFSSSRPGPVHIE
IPTDVMVKPADDIAALLSNAAPPTPDGAAIASAAKLITAARRPLILAGGGAKRAEMALRRLAETLGAPVVETTNARGLLH
RHPLCVPASPSLKAVRALMAEADLVIAAGTEFGPTDYDGYGDGGFVLPSNLIRIDIGADQLARRPVTVGIQADCAEAIEA
LLAAIGTAHPAAPDGEARATAARKAAFAELSPAYLAQVHAVEMIRDALPGAIVVGDSTQPVYAANLYYDHDRPGGWFNAA
TGFGALGYGPPAAIGAALAVPDAPVVCLTGDGGFQFTLPELGAALDAKAPVIFVVWNNRGYREIETSMLDVGVEPVGVSP
APPDFCKLAEAYGIGAERLADIGALPQALKRARATGLPCVIEITVD
>Mature_525_residues
TTLGEALITLLEAHGVDTVFGIPGVHTVELYRGLARSKIRHVTPRHEQGAGFMADGYARASGRPGVAFVITGPGLTNTIT
AMGQARADSVPMLVISGVNATPTLGKGLGFLHELPDQRGMMEKVALLSRRVTEASELPDALAQAFALFSSSRPGPVHIEI
PTDVMVKPADDIAALLSNAAPPTPDGAAIASAAKLITAARRPLILAGGGAKRAEMALRRLAETLGAPVVETTNARGLLHR
HPLCVPASPSLKAVRALMAEADLVIAAGTEFGPTDYDGYGDGGFVLPSNLIRIDIGADQLARRPVTVGIQADCAEAIEAL
LAAIGTAHPAAPDGEARATAARKAAFAELSPAYLAQVHAVEMIRDALPGAIVVGDSTQPVYAANLYYDHDRPGGWFNAAT
GFGALGYGPPAAIGAALAVPDAPVVCLTGDGGFQFTLPELGAALDAKAPVIFVVWNNRGYREIETSMLDVGVEPVGVSPA
PPDFCKLAEAYGIGAERLADIGALPQALKRARATGLPCVIEITVD

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI21361361, Length=579, Percent_Identity=29.1882556131261, Blast_Score=156, Evalue=4e-38,
Organism=Homo sapiens, GI93004078, Length=513, Percent_Identity=25.5360623781676, Blast_Score=125, Evalue=1e-28,
Organism=Escherichia coli, GI87081685, Length=554, Percent_Identity=31.0469314079422, Blast_Score=218, Evalue=8e-58,
Organism=Escherichia coli, GI1790104, Length=539, Percent_Identity=32.2820037105751, Blast_Score=206, Evalue=2e-54,
Organism=Escherichia coli, GI1786717, Length=570, Percent_Identity=28.4210526315789, Blast_Score=155, Evalue=5e-39,
Organism=Escherichia coli, GI1788716, Length=559, Percent_Identity=26.4758497316637, Blast_Score=144, Evalue=2e-35,
Organism=Escherichia coli, GI1787096, Length=539, Percent_Identity=25.417439703154, Blast_Score=102, Evalue=8e-23,
Organism=Caenorhabditis elegans, GI17542570, Length=559, Percent_Identity=28.4436493738819, Blast_Score=152, Evalue=4e-37,
Organism=Caenorhabditis elegans, GI17531299, Length=474, Percent_Identity=23.8396624472574, Blast_Score=87, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17531301, Length=474, Percent_Identity=23.4177215189873, Blast_Score=87, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6323755, Length=587, Percent_Identity=28.9608177172061, Blast_Score=184, Evalue=3e-47,
Organism=Saccharomyces cerevisiae, GI6320816, Length=489, Percent_Identity=23.9263803680982, Blast_Score=102, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6323073, Length=495, Percent_Identity=24.040404040404, Blast_Score=63, Evalue=9e-11,
Organism=Drosophila melanogaster, GI19922626, Length=474, Percent_Identity=27.8481012658228, Blast_Score=147, Evalue=2e-35,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 54308; Mature: 54177

Theoretical pI: Translated: 5.45; Mature: 5.45

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTLGEALITLLEAHGVDTVFGIPGVHTVELYRGLARSKIRHVTPRHEQGAGFMADGYAR
CCCHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEECCCCC
ASGRPGVAFVITGPGLTNTITAMGQARADSVPMLVISGVNATPTLGKGLGFLHELPDQRG
CCCCCCEEEEEECCCCHHHHHHHCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCH
MMEKVALLSRRVTEASELPDALAQAFALFSSSRPGPVHIEIPTDVMVKPADDIAALLSNA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCEEEECHHHHHHHHHCC
APPTPDGAAIASAAKLITAARRPLILAGGGAKRAEMALRRLAETLGAPVVETTNARGLLH
CCCCCCCHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHCCCEEECCCCCCHHH
RHPLCVPASPSLKAVRALMAEADLVIAAGTEFGPTDYDGYGDGGFVLPSNLIRIDIGADQ
CCCEECCCCCCHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCEECCCCEEEEEECHHH
LARRPVTVGIQADCAEAIEALLAAIGTAHPAAPDGEARATAARKAAFAELSPAYLAQVHA
HHCCCEEEEECHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHH
VEMIRDALPGAIVVGDSTQPVYAANLYYDHDRPGGWFNAATGFGALGYGPPAAIGAALAV
HHHHHHHCCCEEEECCCCCCEEEEEEEEECCCCCCCEECCCCCCCCCCCCHHHHHHEEEC
PDAPVVCLTGDGGFQFTLPELGAALDAKAPVIFVVWNNRGYREIETSMLDVGVEPVGVSP
CCCCEEEEECCCCCEEEHHHHCCCCCCCCCEEEEEECCCCCHHHHHHHHHCCCCCCCCCC
APPDFCKLAEAYGIGAERLADIGALPQALKRARATGLPCVIEITVD
CCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEC
>Mature Secondary Structure 
TTLGEALITLLEAHGVDTVFGIPGVHTVELYRGLARSKIRHVTPRHEQGAGFMADGYAR
CCHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEECCCCC
ASGRPGVAFVITGPGLTNTITAMGQARADSVPMLVISGVNATPTLGKGLGFLHELPDQRG
CCCCCCEEEEEECCCCHHHHHHHCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCH
MMEKVALLSRRVTEASELPDALAQAFALFSSSRPGPVHIEIPTDVMVKPADDIAALLSNA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCEEEECHHHHHHHHHCC
APPTPDGAAIASAAKLITAARRPLILAGGGAKRAEMALRRLAETLGAPVVETTNARGLLH
CCCCCCCHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHCCCEEECCCCCCHHH
RHPLCVPASPSLKAVRALMAEADLVIAAGTEFGPTDYDGYGDGGFVLPSNLIRIDIGADQ
CCCEECCCCCCHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCEECCCCEEEEEECHHH
LARRPVTVGIQADCAEAIEALLAAIGTAHPAAPDGEARATAARKAAFAELSPAYLAQVHA
HHCCCEEEEECHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHH
VEMIRDALPGAIVVGDSTQPVYAANLYYDHDRPGGWFNAATGFGALGYGPPAAIGAALAV
HHHHHHHCCCEEEECCCCCCEEEEEEEEECCCCCCCEECCCCCCCCCCCCHHHHHHEEEC
PDAPVVCLTGDGGFQFTLPELGAALDAKAPVIFVVWNNRGYREIETSMLDVGVEPVGVSP
CCCCEEEEECCCCCEEEHHHHCCCCCCCCCEEEEEECCCCCHHHHHHHHHCCCCCCCCCC
APPDFCKLAEAYGIGAERLADIGALPQALKRARATGLPCVIEITVD
CCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6308579; 1630901; 9278503; 3891724; 9298646 [H]