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The map label for this gene is purR [H]

Identifier: 13474304

GI number: 13474304

Start: 4095604

End: 4096581

Strand: Direct

Name: purR [H]

Synonym: mlr5161

Alternate gene names: 13474304

Gene position: 4095604-4096581 (Clockwise)

Preceding gene: 13474303

Following gene: 13474305

Centisome position: 58.21

GC content: 67.79

Gene sequence:

>978_bases
GTGTCCACGGCCACCGTGTCGCTGGCGCTGCGCGACAGTCCGCTGGTGGCCGGCTCGACACGCGACCGCATCAAGGAACA
TGCGCGCGCCATCGGCTATATCTACAACCGCCGCGCCGCCAGCCTCCGCACCTCGCGCTCGGGCATTGTCGGCGTCGTCG
TGCACGACATCATGAACCCGTTCTTCGCCGAGATCCTGCGCTCGATCGAAAGCGAACTCGACCGCAGCCGGCAGACCTTC
ATCCTGTCCAATCATTACGACCAACTCGAAAAACAGCGCACCTTCATCGACACGCTGCTGCAGCTTGGCGCCGACGGCGT
CATCATGTCGCCGGCCATCGGCACTCCGGCCGCCGACATACTGATGGCCGAGGAGAACGGCCTGCCGGCGGTGCTGATCG
CCCGCACCGTCGAGGGCGCCGACGTGCCGGTGTTTCGCGGCGACGATTCCTATGGCACCGGGCTTGCCACCAACCATCTG
ATCTCGCTCGGCCACAAGCGCATCGCCATGATCGGCGGCACCGACCAGACCTCGACCGGCCGCGACCGCTACCAGGGCTA
TGTCAACGCCATGGAGGCGGCAGGGCTCGAAGTCAGGCCGTCCTGGCGCATCGCCGGTCCGCGCACCAAGCAGGCCGGCT
TCGAGGCCGCCGGGCAGTTCCTGGCGCTGAAGGACAGACCGACCGCCGCCTGCTGCTGGAACGACCTCGTTGCCATCGGC
CTGATGAACGGCATTGCGCGCGCCGGCCTGGTGCCGGGCATCGACATCTCCGTCACCGGCTATGACGATCTCGAGGAGGC
GGCGATCGCCACGCCGGCGCTGACCACCGTCTGGAACGGCCAGCGCGAGGTCGGCCGCCGCGCCGCCAGCGCGCTGCTCG
ACAAGCTCAATGGGCAGACGGTGCGGCCCTCGCAGGAATTGATCAAGCCGGAACTGCATGTGCGCCAGTCGACCGGCAAG
CCGGTGGAGCGGGCATGA

Upstream 100 bases:

>100_bases
CGCCGCCTTTAAACTGTTTAGGTCGCAATTCAGCTCGTTCGGGGGGCGCAAGGCGCTGGCACAAAAAATCAAGCTTTCGA
CGATCGCCGATGCACTCGGC

Downstream 100 bases:

>100_bases
CCAAGGCCGAACAACTGCAAGCCGTCGCCATTCTGGTGCCGGCGGACTTCAGCGACCATGCCGTCCGGCGCATCGACCGT
ACCTTCAAACAGGTGAGCAT

Product: transcriptional regulator

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 325; Mature: 324

Protein sequence:

>325_residues
MSTATVSLALRDSPLVAGSTRDRIKEHARAIGYIYNRRAASLRTSRSGIVGVVVHDIMNPFFAEILRSIESELDRSRQTF
ILSNHYDQLEKQRTFIDTLLQLGADGVIMSPAIGTPAADILMAEENGLPAVLIARTVEGADVPVFRGDDSYGTGLATNHL
ISLGHKRIAMIGGTDQTSTGRDRYQGYVNAMEAAGLEVRPSWRIAGPRTKQAGFEAAGQFLALKDRPTAACCWNDLVAIG
LMNGIARAGLVPGIDISVTGYDDLEEAAIATPALTTVWNGQREVGRRAASALLDKLNGQTVRPSQELIKPELHVRQSTGK
PVERA

Sequences:

>Translated_325_residues
MSTATVSLALRDSPLVAGSTRDRIKEHARAIGYIYNRRAASLRTSRSGIVGVVVHDIMNPFFAEILRSIESELDRSRQTF
ILSNHYDQLEKQRTFIDTLLQLGADGVIMSPAIGTPAADILMAEENGLPAVLIARTVEGADVPVFRGDDSYGTGLATNHL
ISLGHKRIAMIGGTDQTSTGRDRYQGYVNAMEAAGLEVRPSWRIAGPRTKQAGFEAAGQFLALKDRPTAACCWNDLVAIG
LMNGIARAGLVPGIDISVTGYDDLEEAAIATPALTTVWNGQREVGRRAASALLDKLNGQTVRPSQELIKPELHVRQSTGK
PVERA
>Mature_324_residues
STATVSLALRDSPLVAGSTRDRIKEHARAIGYIYNRRAASLRTSRSGIVGVVVHDIMNPFFAEILRSIESELDRSRQTFI
LSNHYDQLEKQRTFIDTLLQLGADGVIMSPAIGTPAADILMAEENGLPAVLIARTVEGADVPVFRGDDSYGTGLATNHLI
SLGHKRIAMIGGTDQTSTGRDRYQGYVNAMEAAGLEVRPSWRIAGPRTKQAGFEAAGQFLALKDRPTAACCWNDLVAIGL
MNGIARAGLVPGIDISVTGYDDLEEAAIATPALTTVWNGQREVGRRAASALLDKLNGQTVRPSQELIKPELHVRQSTGKP
VERA

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790369, Length=320, Percent_Identity=32.8125, Blast_Score=171, Evalue=4e-44,
Organism=Escherichia coli, GI1787948, Length=320, Percent_Identity=29.375, Blast_Score=147, Evalue=1e-36,
Organism=Escherichia coli, GI1790194, Length=318, Percent_Identity=28.3018867924528, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI1787906, Length=328, Percent_Identity=28.6585365853659, Blast_Score=130, Evalue=9e-32,
Organism=Escherichia coli, GI1789202, Length=280, Percent_Identity=30.7142857142857, Blast_Score=123, Evalue=1e-29,
Organism=Escherichia coli, GI1786540, Length=323, Percent_Identity=27.8637770897833, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1788474, Length=278, Percent_Identity=28.0575539568345, Blast_Score=104, Evalue=9e-24,
Organism=Escherichia coli, GI48994940, Length=309, Percent_Identity=28.1553398058252, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1787580, Length=323, Percent_Identity=24.7678018575851, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1789068, Length=309, Percent_Identity=25.2427184466019, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1790715, Length=304, Percent_Identity=27.3026315789474, Blast_Score=82, Evalue=6e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 35044; Mature: 34913

Theoretical pI: Translated: 7.67; Mature: 7.67

Prosite motif: PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTATVSLALRDSPLVAGSTRDRIKEHARAIGYIYNRRAASLRTSRSGIVGVVVHDIMNP
CCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
FFAEILRSIESELDRSRQTFILSNHYDQLEKQRTFIDTLLQLGADGVIMSPAIGTPAADI
HHHHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCHHHE
LMAEENGLPAVLIARTVEGADVPVFRGDDSYGTGLATNHLISLGHKRIAMIGGTDQTSTG
EEECCCCCCEEEEEEECCCCCCCEEECCCCCCCCHHHHHHHHHCCCEEEEECCCCCCCCC
RDRYQGYVNAMEAAGLEVRPSWRIAGPRTKQAGFEAAGQFLALKDRPTAACCWNDLVAIG
HHHHHHHHHHHHHCCCEECCCEEECCCCCHHHCHHHHCCEEEECCCCCHHHHHHHHHHHH
LMNGIARAGLVPGIDISVTGYDDLEEAAIATPALTTVWNGQREVGRRAASALLDKLNGQT
HHHHHHHHCCCCCCEEEECCCCCHHHHHHCCCHHHHHCCCHHHHHHHHHHHHHHHHCCCC
VRPSQELIKPELHVRQSTGKPVERA
CCCHHHHHCCHHHEECCCCCCCCCC
>Mature Secondary Structure 
STATVSLALRDSPLVAGSTRDRIKEHARAIGYIYNRRAASLRTSRSGIVGVVVHDIMNP
CCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
FFAEILRSIESELDRSRQTFILSNHYDQLEKQRTFIDTLLQLGADGVIMSPAIGTPAADI
HHHHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCHHHE
LMAEENGLPAVLIARTVEGADVPVFRGDDSYGTGLATNHLISLGHKRIAMIGGTDQTSTG
EEECCCCCCEEEEEEECCCCCCCEEECCCCCCCCHHHHHHHHHCCCEEEEECCCCCCCCC
RDRYQGYVNAMEAAGLEVRPSWRIAGPRTKQAGFEAAGQFLALKDRPTAACCWNDLVAIG
HHHHHHHHHHHHHCCCEECCCEEECCCCCHHHCHHHHCCEEEECCCCCHHHHHHHHHHHH
LMNGIARAGLVPGIDISVTGYDDLEEAAIATPALTTVWNGQREVGRRAASALLDKLNGQT
HHHHHHHHCCCCCCEEEECCCCCHHHHHHCCCHHHHHCCCHHHHHHHHHHHHHHHHCCCC
VRPSQELIKPELHVRQSTGKPVERA
CCCHHHHHCCHHHEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA