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The map label for this gene is pfp [H]

Identifier: 13474191

GI number: 13474191

Start: 3978826

End: 3979992

Strand: Reverse

Name: pfp [H]

Synonym: mll5025

Alternate gene names: 13474191

Gene position: 3979992-3978826 (Counterclockwise)

Preceding gene: 13474192

Following gene: 13474190

Centisome position: 56.57

GC content: 66.41

Gene sequence:

>1167_bases
ATGGCCGGAACTTTTATCATCGCGCAGGGCGGCGGCCCGACCGCCGTCATCAACCAGACGGTGGTCGGCGCCACGCTGGA
GATCCGCAAGCGGCATCCGGGCGCCAAGGTCCTGGGCTCCATCCATGGCGTGCGCGGCATCCGCGACGGCAATTATGTCG
ACCTCTCGGCCATCCCCGAGGATCGGCTGCGGCTGATAGCGGGAACGCCAAGCGCCGCCCTTGGCTCGACGCGCGACAAG
CCGGATGCCGCCTATTGCGACGTCATCCTCAAAGGCCTGAAGAAGGCCGGCGCCGACGCCTTCATCTATATCGGCGGCAA
CGACACGTCGGGCACGCAGCAGATCCTGACCGATGCCGCCGGCGGCTCGATTGCTTTCGTCCATGCACCCAAGACCATCG
ACAATGATCTGGAGGAGAACGACCACACGCCGGGCTTCATCTCGGCGGCCGAGTTCGTCGCCGGCGCCTTCCTCTCCGTC
GATCTCGATTTCCGCGCTTTGCCTGGCATCTATGTCGGCATCGTCATGGGCCGGCATGCCGGTTTTCTCACCGCGGCGGC
GGCCGCCTGGCAGCTCGATCCCGACAGCGGCCCGCATCTGGTCTATGTGCCGGAGCGGCCGTTCTCGGCCGCCGGCTTCA
TCGATGATGTGCGCGCCACGCTTGACCGGCACAAGCGCTGCATCGTCGCCGTGTCGGAAGGGGTAAGCACCGCCGACGGC
AAGGCGCTGGTCGAAAGCCTGGTGCCGCCGGAGAAGCTCGAGCGTGACGCCCATGGTAACGTCAAGCTGTCGGGCAGCGA
CCTGCCAGCCGCGCTTGAGCGCGCGCTGGCCGAAGGCCTGCCCGGCAAGCGGGCCCGCGTCGACGCGCTCGGCTACATGC
CGCGCGGCTATGTCGGCGCCATCAGCGCCGTCGACGCGCAGGAGGCGTTCGATGCCGGCGCCTTCGCCGTCTCCGTCGCC
GAACAGGGCGGCGGCTCGGTGGCGCTGCAGTATGACGGGACAAAGACGGTGCTGAAAAAGGTGCCGCTGAAGAATGTCGC
CGGCAAGACCCGCCACATGCCTGACGATTTCATGAAGCCCGACGTCAACCAGCTGTCCGAAGCCGGCATGGCCTATCTCA
AGCGGCTGGTGCCGGAAAAGTACAAGGTCGGGAAGCCTTTCGTTTAA

Upstream 100 bases:

>100_bases
TCGTCGTCATGTGATCCTTTATCCCTCTGTTTCTCCCGTTGCGTGATGCGGCAAGACAGGCCAAGGTGCCTTGAAAACCA
TCGCTCGGGAGACGTCAGGA

Downstream 100 bases:

>100_bases
TGGAGTGGTTCAGCAAGAGCATCGTCGACGACAGGACGACGATGCTGACCGAGCCGTTCGTGCATGACTATGTGCGCGCC
AACATCTGGCATCTGCGCGG

Product: 6-phosphofructokinase

Products: NA

Alternate protein names: 6-phosphofructokinase, pyrophosphate dependent; PPi-dependent phosphofructokinase; PPi-PFK; Pyrophosphate-dependent 6-phosphofructose-1-kinase [H]

Number of amino acids: Translated: 388; Mature: 387

Protein sequence:

>388_residues
MAGTFIIAQGGGPTAVINQTVVGATLEIRKRHPGAKVLGSIHGVRGIRDGNYVDLSAIPEDRLRLIAGTPSAALGSTRDK
PDAAYCDVILKGLKKAGADAFIYIGGNDTSGTQQILTDAAGGSIAFVHAPKTIDNDLEENDHTPGFISAAEFVAGAFLSV
DLDFRALPGIYVGIVMGRHAGFLTAAAAAWQLDPDSGPHLVYVPERPFSAAGFIDDVRATLDRHKRCIVAVSEGVSTADG
KALVESLVPPEKLERDAHGNVKLSGSDLPAALERALAEGLPGKRARVDALGYMPRGYVGAISAVDAQEAFDAGAFAVSVA
EQGGGSVALQYDGTKTVLKKVPLKNVAGKTRHMPDDFMKPDVNQLSEAGMAYLKRLVPEKYKVGKPFV

Sequences:

>Translated_388_residues
MAGTFIIAQGGGPTAVINQTVVGATLEIRKRHPGAKVLGSIHGVRGIRDGNYVDLSAIPEDRLRLIAGTPSAALGSTRDK
PDAAYCDVILKGLKKAGADAFIYIGGNDTSGTQQILTDAAGGSIAFVHAPKTIDNDLEENDHTPGFISAAEFVAGAFLSV
DLDFRALPGIYVGIVMGRHAGFLTAAAAAWQLDPDSGPHLVYVPERPFSAAGFIDDVRATLDRHKRCIVAVSEGVSTADG
KALVESLVPPEKLERDAHGNVKLSGSDLPAALERALAEGLPGKRARVDALGYMPRGYVGAISAVDAQEAFDAGAFAVSVA
EQGGGSVALQYDGTKTVLKKVPLKNVAGKTRHMPDDFMKPDVNQLSEAGMAYLKRLVPEKYKVGKPFV
>Mature_387_residues
AGTFIIAQGGGPTAVINQTVVGATLEIRKRHPGAKVLGSIHGVRGIRDGNYVDLSAIPEDRLRLIAGTPSAALGSTRDKP
DAAYCDVILKGLKKAGADAFIYIGGNDTSGTQQILTDAAGGSIAFVHAPKTIDNDLEENDHTPGFISAAEFVAGAFLSVD
LDFRALPGIYVGIVMGRHAGFLTAAAAAWQLDPDSGPHLVYVPERPFSAAGFIDDVRATLDRHKRCIVAVSEGVSTADGK
ALVESLVPPEKLERDAHGNVKLSGSDLPAALERALAEGLPGKRARVDALGYMPRGYVGAISAVDAQEAFDAGAFAVSVAE
QGGGSVALQYDGTKTVLKKVPLKNVAGKTRHMPDDFMKPDVNQLSEAGMAYLKRLVPEKYKVGKPFV

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family [H]

Homologues:

Organism=Escherichia coli, GI1790350, Length=247, Percent_Identity=29.9595141700405, Blast_Score=68, Evalue=8e-13,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012003
- InterPro:   IPR012829
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023 [H]

Pfam domain/function: PF00365 PFK [H]

EC number: =2.7.1.90 [H]

Molecular weight: Translated: 40651; Mature: 40520

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGTFIIAQGGGPTAVINQTVVGATLEIRKRHPGAKVLGSIHGVRGIRDGNYVDLSAIPE
CCCEEEEECCCCCEEEECCEEECCEEEEHHCCCCHHHHHHHHHHCCCCCCCEEEEECCCC
DRLRLIAGTPSAALGSTRDKPDAAYCDVILKGLKKAGADAFIYIGGNDTSGTQQILTDAA
CCEEEEECCCCHHCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHCC
GGSIAFVHAPKTIDNDLEENDHTPGFISAAEFVAGAFLSVDLDFRALPGIYVGIVMGRHA
CCCEEEEECCCHHCCCHHCCCCCCCHHHHHHHHHHEEEEEECCHHHCCCCEEEHHHHCCC
GFLTAAAAAWQLDPDSGPHLVYVPERPFSAAGFIDDVRATLDRHKRCIVAVSEGVSTADG
CHHHHHHHEEECCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCEEEEEEECCCCCCCH
KALVESLVPPEKLERDAHGNVKLSGSDLPAALERALAEGLPGKRARVDALGYMPRGYVGA
HHHHHHCCCHHHHCCCCCCCEEECCCCCHHHHHHHHHHCCCCCCEEEHHHCCCCCCHHHH
ISAVDAQEAFDAGAFAVSVAEQGGGSVALQYDGTKTVLKKVPLKNVAGKTRHMPDDFMKP
HHHCCHHHHHCCCCEEEEEECCCCCEEEEEECCHHHHHHHCCCHHHCCCCCCCCHHHCCC
DVNQLSEAGMAYLKRLVPEKYKVGKPFV
CHHHHHHHHHHHHHHHCCHHHCCCCCCC
>Mature Secondary Structure 
AGTFIIAQGGGPTAVINQTVVGATLEIRKRHPGAKVLGSIHGVRGIRDGNYVDLSAIPE
CCEEEEECCCCCEEEECCEEECCEEEEHHCCCCHHHHHHHHHHCCCCCCCEEEEECCCC
DRLRLIAGTPSAALGSTRDKPDAAYCDVILKGLKKAGADAFIYIGGNDTSGTQQILTDAA
CCEEEEECCCCHHCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHCC
GGSIAFVHAPKTIDNDLEENDHTPGFISAAEFVAGAFLSVDLDFRALPGIYVGIVMGRHA
CCCEEEEECCCHHCCCHHCCCCCCCHHHHHHHHHHEEEEEECCHHHCCCCEEEHHHHCCC
GFLTAAAAAWQLDPDSGPHLVYVPERPFSAAGFIDDVRATLDRHKRCIVAVSEGVSTADG
CHHHHHHHEEECCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCEEEEEEECCCCCCCH
KALVESLVPPEKLERDAHGNVKLSGSDLPAALERALAEGLPGKRARVDALGYMPRGYVGA
HHHHHHCCCHHHHCCCCCCCEEECCCCCHHHHHHHHHHCCCCCCEEEHHHCCCCCCHHHH
ISAVDAQEAFDAGAFAVSVAEQGGGSVALQYDGTKTVLKKVPLKNVAGKTRHMPDDFMKP
HHHCCHHHHHCCCCEEEEEECCCCCEEEEEECCHHHHHHHCCCHHHCCCCCCCCHHHCCC
DVNQLSEAGMAYLKRLVPEKYKVGKPFV
CHHHHHHHHHHHHHHHCCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8550409 [H]