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The map label for this gene is mscL

Identifier: 13473952

GI number: 13473952

Start: 3743790

End: 3744212

Strand: Direct

Name: mscL

Synonym: mlr4713

Alternate gene names: 13473952

Gene position: 3743790-3744212 (Clockwise)

Preceding gene: 13473950

Following gene: 13473954

Centisome position: 53.21

GC content: 52.48

Gene sequence:

>423_bases
ATGCTCAAGGAGTTTCAGGAATTCATTTCCAAGGGCAATGTGATGGACTTGGCTGTAGGCGTCATCATCGGCGCAGCTTT
CGGTAAGATCGTTGATTCACTGGTCAACGACATCATCATGCCGGTCATAGGTGCAATTTTCGGCGGACTGGATTTCAATA
ATTATTTCGTCGGGCTTTCTTCAGCGGTCAATGCCACTTCGCTGGCCGACGCCAAAAAGCAAGGCGCCGTCTTTGCCTAC
GGCAGCTTCATTACCGTGGCGTTGAATTTTGTCATTCTCGCCTTCATCATCTTCCTTATGGTCAAGGCTGTGAACAATCT
GCGTAGAAGGCTCGAGCGTGAAAAGCCGGCAACACCAGCGGCTCCACCGCCCGCCGACGTCGCGCTACTCACCGAAATTC
GCGACCTCCTCGCAAGGAGATAG

Upstream 100 bases:

>100_bases
GCCGTCCAGTCACATAGGCTCCGACGTCGCGAAACCCGTAGACGTTGGGGCAATCTCAGTTCAATCTCTCGAACAGGCAG
TTAACCCGGAGGATACGAGA

Downstream 100 bases:

>100_bases
CCAAAAGCTGTCGAGGACTCAGGTTATTCGCAGCCGCACAGTTGGAAAGGGATGGCAGCCGCCAACGCCGGTTAGGCGTC
GACCGAAGGTATTGCCCGGA

Product: large-conductance mechanosensitive channel

Products: non-specific ion/solute [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 140; Mature: 140

Protein sequence:

>140_residues
MLKEFQEFISKGNVMDLAVGVIIGAAFGKIVDSLVNDIIMPVIGAIFGGLDFNNYFVGLSSAVNATSLADAKKQGAVFAY
GSFITVALNFVILAFIIFLMVKAVNNLRRRLEREKPATPAAPPPADVALLTEIRDLLARR

Sequences:

>Translated_140_residues
MLKEFQEFISKGNVMDLAVGVIIGAAFGKIVDSLVNDIIMPVIGAIFGGLDFNNYFVGLSSAVNATSLADAKKQGAVFAY
GSFITVALNFVILAFIIFLMVKAVNNLRRRLEREKPATPAAPPPADVALLTEIRDLLARR
>Mature_140_residues
MLKEFQEFISKGNVMDLAVGVIIGAAFGKIVDSLVNDIIMPVIGAIFGGLDFNNYFVGLSSAVNATSLADAKKQGAVFAY
GSFITVALNFVILAFIIFLMVKAVNNLRRRLEREKPATPAAPPPADVALLTEIRDLLARR

Specific function: Channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell

COG id: COG1970

COG function: function code M; Large-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscL family

Homologues:

Organism=Escherichia coli, GI1789686, Length=142, Percent_Identity=50, Blast_Score=122, Evalue=1e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MSCL2_RHILO (Q98DG5)

Other databases:

- EMBL:   BA000012
- RefSeq:   NP_105520.1
- ProteinModelPortal:   Q98DG5
- SMR:   Q98DG5
- GeneID:   1228181
- GenomeReviews:   BA000012_GR
- KEGG:   mlo:mlr4713
- NMPDR:   fig|266835.1.peg.3624
- HOGENOM:   HBG727604
- OMA:   IQLLTEI
- ProtClustDB:   PRK13952
- HAMAP:   MF_00115
- InterPro:   IPR019823
- InterPro:   IPR001185
- InterPro:   IPR015089
- Gene3D:   G3DSA:1.20.5.220
- PRINTS:   PR01264
- TIGRFAMs:   TIGR00220

Pfam domain/function: PF01741 MscL; SSF81330 MS_channel

EC number: NA

Molecular weight: Translated: 15086; Mature: 15086

Theoretical pI: Translated: 9.69; Mature: 9.69

Prosite motif: PS01327 MSCL

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x13df25c8)-; HASH(0x1054b338)-; HASH(0x1359e300)-;

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKEFQEFISKGNVMDLAVGVIIGAAFGKIVDSLVNDIIMPVIGAIFGGLDFNNYFVGLS
CHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
SAVNATSLADAKKQGAVFAYGSFITVALNFVILAFIIFLMVKAVNNLRRRLEREKPATPA
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
APPPADVALLTEIRDLLARR
CCCCHHHHHHHHHHHHHCCH
>Mature Secondary Structure
MLKEFQEFISKGNVMDLAVGVIIGAAFGKIVDSLVNDIIMPVIGAIFGGLDFNNYFVGLS
CHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
SAVNATSLADAKKQGAVFAYGSFITVALNFVILAFIIFLMVKAVNNLRRRLEREKPATPA
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
APPPADVALLTEIRDLLARR
CCCCHHHHHHHHHHHHHCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: non-specific ion/solute [Cytoplasm] [C]

Specific reaction: non-specific ion/solute [Cytoplasm] = non-specific ion/solute [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11214968