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The map label for this gene is ydfI [H]

Identifier: 13473454

GI number: 13473454

Start: 3243390

End: 3244889

Strand: Direct

Name: ydfI [H]

Synonym: mlr4057

Alternate gene names: 13473454

Gene position: 3243390-3244889 (Clockwise)

Preceding gene: 13473450

Following gene: 13473464

Centisome position: 46.1

GC content: 65.8

Gene sequence:

>1500_bases
ATGCTGATGACCAGAAGCCATCTGTCCGACGCCACGCTACGCAAGCTGCCCGCCGGGACCAAAATCCCTGCATATGACCG
CGGCCGCATTGCTCCGGGCATCGTGCATCTCGGCGTCGGCGCCTTCCATCGCGCCCATCAGGCCGCCTATGTCGACGAGT
GCCTGGCGGCGGGCGAAACGGACTGGGGCATAACAGGCGTCTCCTTGCGCAGCCCCGACACGCGCGATGCGCTGTCGCCG
CAGGACGGGCTCTACACGCTGGCGATCCGCAGCAGTGGCGAGGAGGAGCTGTGCGTCATCGGCTCTATCCAGTCGATGCT
CGTGGCGCCGGAAAACCCCAGCGCGGTGCTGGCCGCGCTGACCGATCCACGCACCCGCATCGTCACGCTGACCATCACCG
AAAAGGCCTATCTCAGGGCTGCGGGCGGCGGATTGGACGCCGCCCATCCCGACATCATCCACGATCTGGCCAATCCGCAA
ATGCCGAGGACGGCGCATGGTTTCCTCGCCGAGGCGCTTGCCCGCAGACTGGCCGCCGGCACCCCGCCCTTCACCGTGCT
GTGCTGCGACAACCTGCCGGCCAACGGCGCCACGCTGCATCGGCTGCTGACCGAATTCGCCAAGCTGCGCGATGCCAGGT
CTACCACTGGAGGCGACACCAGAATCGCCGACCATATCGCGAACCAGGTCGCCTTTCCGTCGAGCATGGTCGATCGCATC
GTGCCGGCAACCACCGACGCCGACCGGGCGCGCATCGCTGGCGAACTCGGTGTCGACGATGCCTGGCCTGTCATGACCGA
GCCCTTCCGCCAATGGGTGGTCGAAGACAATTTCTCCGCCGGCAGGCCGGACTGGGAAAAGTTCGGCGTCACCATGGTTG
GCGATGTCGGCCCCTTCGAGGACATGAAGCTCAGGCTGCTCAACGGCGCGCATTCGGGCATCGCCTATCTCGGCCTGCTC
AGCGGCCACGCCACCGTCGACCGCGCCTTTGCCGATCCGGCGATCCGGCAATTTGTCGACGGGCTCTGGGCCGAGGCCAT
CCCGACATTGCCGGAGGATGCCGGACTCGATACGGCAACCTACACAGCCGAGCTTGCCGAGCGTTTCTCCAACACCGCGC
TTGCCCACCGCACGGCGCAGATCGCCAATGACGGCAGCCAGAAACTGCCGCAGCGCATCATCGCGTCCGCCATCGAGTGC
CTCGAAGCCGGCACCGAATTCGTGCATCTCACCCTGGTGGTGGCGGCCTGGATCGCCGCCTGCGCCGCGCGCGGAAAAAC
CTTGCCTGAAAACCATTTCACCGATCCGCTCGATGCCGCGCTGAGGGCGCTTTTGGACCAGCAATTGCCGGCGAACGAAA
CCGTGACGGCGGTGTTCGACCTGGCCGGCTTTGCCAGGGACAACGCCGAACGCCAGACGCTGATCGAGCTCACGGCGGTG
CATCTTGTGCACTTGCGGCGCGATGGTCCGACATTGGCCTTCGCCGCCTTGGGCATTTAA

Upstream 100 bases:

>100_bases
CGGGCCAGAGCGATGTCAATGTGCGTTGGCAAAGCGGCATCCTTGCGGCAGGCGCAAACAGGTGTTACCCCGCCTGTCAT
CCACAGCCCCCAGCCCCGGT

Downstream 100 bases:

>100_bases
ACAAAAAAGCGGGGCAAGGCCCCGCTTTTCATCAAGTCATCTGGTGCGGATTTCAGGCCGGCAGAATTGCGCCTTCCAGC
GTGGTCAGCTGGCTGAGGAA

Product: mannitol dehydrogenase

Products: D-fructose; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 499; Mature: 499

Protein sequence:

>499_residues
MLMTRSHLSDATLRKLPAGTKIPAYDRGRIAPGIVHLGVGAFHRAHQAAYVDECLAAGETDWGITGVSLRSPDTRDALSP
QDGLYTLAIRSSGEEELCVIGSIQSMLVAPENPSAVLAALTDPRTRIVTLTITEKAYLRAAGGGLDAAHPDIIHDLANPQ
MPRTAHGFLAEALARRLAAGTPPFTVLCCDNLPANGATLHRLLTEFAKLRDARSTTGGDTRIADHIANQVAFPSSMVDRI
VPATTDADRARIAGELGVDDAWPVMTEPFRQWVVEDNFSAGRPDWEKFGVTMVGDVGPFEDMKLRLLNGAHSGIAYLGLL
SGHATVDRAFADPAIRQFVDGLWAEAIPTLPEDAGLDTATYTAELAERFSNTALAHRTAQIANDGSQKLPQRIIASAIEC
LEAGTEFVHLTLVVAAWIAACAARGKTLPENHFTDPLDAALRALLDQQLPANETVTAVFDLAGFARDNAERQTLIELTAV
HLVHLRRDGPTLAFAALGI

Sequences:

>Translated_499_residues
MLMTRSHLSDATLRKLPAGTKIPAYDRGRIAPGIVHLGVGAFHRAHQAAYVDECLAAGETDWGITGVSLRSPDTRDALSP
QDGLYTLAIRSSGEEELCVIGSIQSMLVAPENPSAVLAALTDPRTRIVTLTITEKAYLRAAGGGLDAAHPDIIHDLANPQ
MPRTAHGFLAEALARRLAAGTPPFTVLCCDNLPANGATLHRLLTEFAKLRDARSTTGGDTRIADHIANQVAFPSSMVDRI
VPATTDADRARIAGELGVDDAWPVMTEPFRQWVVEDNFSAGRPDWEKFGVTMVGDVGPFEDMKLRLLNGAHSGIAYLGLL
SGHATVDRAFADPAIRQFVDGLWAEAIPTLPEDAGLDTATYTAELAERFSNTALAHRTAQIANDGSQKLPQRIIASAIEC
LEAGTEFVHLTLVVAAWIAACAARGKTLPENHFTDPLDAALRALLDQQLPANETVTAVFDLAGFARDNAERQTLIELTAV
HLVHLRRDGPTLAFAALGI
>Mature_499_residues
MLMTRSHLSDATLRKLPAGTKIPAYDRGRIAPGIVHLGVGAFHRAHQAAYVDECLAAGETDWGITGVSLRSPDTRDALSP
QDGLYTLAIRSSGEEELCVIGSIQSMLVAPENPSAVLAALTDPRTRIVTLTITEKAYLRAAGGGLDAAHPDIIHDLANPQ
MPRTAHGFLAEALARRLAAGTPPFTVLCCDNLPANGATLHRLLTEFAKLRDARSTTGGDTRIADHIANQVAFPSSMVDRI
VPATTDADRARIAGELGVDDAWPVMTEPFRQWVVEDNFSAGRPDWEKFGVTMVGDVGPFEDMKLRLLNGAHSGIAYLGLL
SGHATVDRAFADPAIRQFVDGLWAEAIPTLPEDAGLDTATYTAELAERFSNTALAHRTAQIANDGSQKLPQRIIASAIEC
LEAGTEFVHLTLVVAAWIAACAARGKTLPENHFTDPLDAALRALLDQQLPANETVTAVFDLAGFARDNAERQTLIELTAV
HLVHLRRDGPTLAFAALGI

Specific function: Unknown

COG id: COG0246

COG function: function code G; Mannitol-1-phosphate/altronate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mannitol dehydrogenase family. UxuB subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787823, Length=426, Percent_Identity=44.8356807511737, Blast_Score=330, Evalue=1e-91,
Organism=Escherichia coli, GI1790779, Length=453, Percent_Identity=42.6048565121413, Blast_Score=323, Evalue=1e-89,
Organism=Escherichia coli, GI1788497, Length=450, Percent_Identity=42.4444444444444, Blast_Score=316, Evalue=2e-87,
Organism=Escherichia coli, GI48994885, Length=412, Percent_Identity=27.9126213592233, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI1790028, Length=262, Percent_Identity=26.7175572519084, Blast_Score=77, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6324401, Length=446, Percent_Identity=40.5829596412556, Blast_Score=334, Evalue=2e-92,
Organism=Saccharomyces cerevisiae, GI6320765, Length=446, Percent_Identity=40.5829596412556, Blast_Score=334, Evalue=2e-92,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008927
- InterPro:   IPR013328
- InterPro:   IPR000669
- InterPro:   IPR013118
- InterPro:   IPR023027
- InterPro:   IPR013131
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01232 Mannitol_dh; PF08125 Mannitol_dh_C [H]

EC number: 1.1.1.67

Molecular weight: Translated: 53392; Mature: 53392

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: PS00974 MANNITOL_DHGENASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLMTRSHLSDATLRKLPAGTKIPAYDRGRIAPGIVHLGVGAFHRAHQAAYVDECLAAGET
CCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCHHHHCHHHHHHHHHHHHHHHHHHCCCC
DWGITGVSLRSPDTRDALSPQDGLYTLAIRSSGEEELCVIGSIQSMLVAPENPSAVLAAL
CCCEEEEEECCCCCCCCCCCCCCEEEEEEECCCCCCEEEEECHHHHEECCCCCCEEEEEE
TDPRTRIVTLTITEKAYLRAAGGGLDAAHPDIIHDLANPQMPRTAHGFLAEALARRLAAG
CCCCEEEEEEEEECHHHHHCCCCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCC
TPPFTVLCCDNLPANGATLHRLLTEFAKLRDARSTTGGDTRIADHIANQVAFPSSMVDRI
CCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHH
VPATTDADRARIAGELGVDDAWPVMTEPFRQWVVEDNFSAGRPDWEKFGVTMVGDVGPFE
CCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHCCEEEEECCCCCH
DMKLRLLNGAHSGIAYLGLLSGHATVDRAFADPAIRQFVDGLWAEAIPTLPEDAGLDTAT
HHHHHHHCCCCCCHHHHHHHCCCHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCHHH
YTAELAERFSNTALAHRTAQIANDGSQKLPQRIIASAIECLEAGTEFVHLTLVVAAWIAA
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
CAARGKTLPENHFTDPLDAALRALLDQQLPANETVTAVFDLAGFARDNAERQTLIELTAV
HHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHH
HLVHLRRDGPTLAFAALGI
HHHHHCCCCCEEEEEECCC
>Mature Secondary Structure
MLMTRSHLSDATLRKLPAGTKIPAYDRGRIAPGIVHLGVGAFHRAHQAAYVDECLAAGET
CCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCHHHHCHHHHHHHHHHHHHHHHHHCCCC
DWGITGVSLRSPDTRDALSPQDGLYTLAIRSSGEEELCVIGSIQSMLVAPENPSAVLAAL
CCCEEEEEECCCCCCCCCCCCCCEEEEEEECCCCCCEEEEECHHHHEECCCCCCEEEEEE
TDPRTRIVTLTITEKAYLRAAGGGLDAAHPDIIHDLANPQMPRTAHGFLAEALARRLAAG
CCCCEEEEEEEEECHHHHHCCCCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCC
TPPFTVLCCDNLPANGATLHRLLTEFAKLRDARSTTGGDTRIADHIANQVAFPSSMVDRI
CCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHH
VPATTDADRARIAGELGVDDAWPVMTEPFRQWVVEDNFSAGRPDWEKFGVTMVGDVGPFE
CCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHCCEEEEECCCCCH
DMKLRLLNGAHSGIAYLGLLSGHATVDRAFADPAIRQFVDGLWAEAIPTLPEDAGLDTAT
HHHHHHHCCCCCCHHHHHHHCCCHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCHHH
YTAELAERFSNTALAHRTAQIANDGSQKLPQRIIASAIECLEAGTEFVHLTLVVAAWIAA
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
CAARGKTLPENHFTDPLDAALRALLDQQLPANETVTAVFDLAGFARDNAERQTLIELTAV
HHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHH
HLVHLRRDGPTLAFAALGI
HHHHHCCCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: D-mannitol; NAD+

Specific reaction: D-mannitol + NAD+ = D-fructose + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]