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The map label for this gene is pcpB [H]

Identifier: 13473298

GI number: 13473298

Start: 3087027

End: 3088277

Strand: Reverse

Name: pcpB [H]

Synonym: mll3850

Alternate gene names: 13473298

Gene position: 3088277-3087027 (Counterclockwise)

Preceding gene: 13473304

Following gene: 13473295

Centisome position: 43.89

GC content: 62.03

Gene sequence:

>1251_bases
ATGTTGCCCGATATGACTGAAATTCTGATCATCGGTGCCGGTCCGACCGGCATGGCGTTGTCGATTGCTTTGCACCAGGC
CAATGTCGATCACGTGCTCATCGAAAAGCTTGCGCATGGTCTGCAGACGTCGCGCGCCGGTGTCATCCATGCACAGACGC
TGGAATCGCTTGAGCCACTCGGCGTCACTCAGCGGCTGAGTGAGCTTTCCGTGAAGCTGGAAGACTTCGCCATACGCGAC
CGAAACAGAGCGCTGCTCAAGCTAGATTTCTGCAAGCTCCCATCAAGGCATCCCTATCTGATGATGCTGCCGCAGAACCT
GACCGAGCAGGTGTTTGCCGAGCGGATCTCCGCACTTGGCGGCACCATTCATCGCGAAGTCGAGGCTGTGGCGGTTGCTC
AAGACAGTGTCGGCGCGCGCGTGACAGTGGTCCAAAACGGCCAAGAGAAGATCATATCGGCTCGCTACGTCGTCGGTGCC
GACGGCATGCAAAGCCTGGTGCGCAAGTCGACAGGCATCGAGTTCGACGGCGCGGCCTATGACGGCTCGTTTGTGCTGGC
GGACGTTCGTCTCGACTGGCCGGTGGGGCCGACAGAGGTGTCGCTGTTCTTTGCCCCGGCGGGACTGGTGGTGGTGGCGC
CACTTCCGGACGGATCATATCGCGTCGTCGCGACAATGGATGAGGCCCCGGAAAAGCCTGATGTCGCCGACATCCAGGCG
CTGCTCGACAGCCGCGGACCGACCAGAAACAAAGCCCGGGTGCTTGGCATCGGCTGGAGTTCGCGATTCCGCCTTCACCA
TCGCCTTGTCCGGGCATACCGCAAGGACCGACTATTCCTGATGGGCGATGCGGCTCATGTCCACAGCCCTGCGGGTGGGC
AAGGCATGAACACAGGCATCATCGATGCGGTCGTGCTTGGCCAGTTGCTTGGCGATGTGGTCAACGGCGTTCGCTCGGAG
GCGGAACTCGACCTCTATGAAACGCTGCGCCGCCCGGCTGCTCAGGAGGTACTCGACCTCGCGGGCAGGATGACCGACAT
GGCACTTGCTCGCAATCCGGTGAAGCGGTTCCTGCGCAATCTGGCGCTCTCCGTGGTGGACCTCAGCCCGTTCCTGAAGC
GTCGCATCGCGCTGAAACTGTCAGGGCTCAGCCGTGCGCCGCTCGCGCGGCTGCCCGCGCCTTCAGCTATGCCGGGGGCA
GTCGAGGCCAGCAAGTCCGAAACCGGACCCAAACTAAAACTCGCCGCCTGA

Upstream 100 bases:

>100_bases
CGTCTGATTCTGGGTCATGAAATGCATATGCCAACGGCTGTTGACAAATTCAACGGCTCCAATTAGAAAGTCAACACATG
TTGACAAATGGAGAAGGAAA

Downstream 100 bases:

>100_bases
CAATGTCGATTGTCAGGCGAGCAGACCTCTGCCGAGCCTCCGGAAGCCTCAGACGAAGTACTGGCCGCCATTGGCCGTGA
TGGTCGAGCCGGTGATGAAG

Product: pentachlorophenol 4-monooxygenase; PcpB

Products: NA

Alternate protein names: Pentachlorophenol hydroxylase [H]

Number of amino acids: Translated: 416; Mature: 416

Protein sequence:

>416_residues
MLPDMTEILIIGAGPTGMALSIALHQANVDHVLIEKLAHGLQTSRAGVIHAQTLESLEPLGVTQRLSELSVKLEDFAIRD
RNRALLKLDFCKLPSRHPYLMMLPQNLTEQVFAERISALGGTIHREVEAVAVAQDSVGARVTVVQNGQEKIISARYVVGA
DGMQSLVRKSTGIEFDGAAYDGSFVLADVRLDWPVGPTEVSLFFAPAGLVVVAPLPDGSYRVVATMDEAPEKPDVADIQA
LLDSRGPTRNKARVLGIGWSSRFRLHHRLVRAYRKDRLFLMGDAAHVHSPAGGQGMNTGIIDAVVLGQLLGDVVNGVRSE
AELDLYETLRRPAAQEVLDLAGRMTDMALARNPVKRFLRNLALSVVDLSPFLKRRIALKLSGLSRAPLARLPAPSAMPGA
VEASKSETGPKLKLAA

Sequences:

>Translated_416_residues
MLPDMTEILIIGAGPTGMALSIALHQANVDHVLIEKLAHGLQTSRAGVIHAQTLESLEPLGVTQRLSELSVKLEDFAIRD
RNRALLKLDFCKLPSRHPYLMMLPQNLTEQVFAERISALGGTIHREVEAVAVAQDSVGARVTVVQNGQEKIISARYVVGA
DGMQSLVRKSTGIEFDGAAYDGSFVLADVRLDWPVGPTEVSLFFAPAGLVVVAPLPDGSYRVVATMDEAPEKPDVADIQA
LLDSRGPTRNKARVLGIGWSSRFRLHHRLVRAYRKDRLFLMGDAAHVHSPAGGQGMNTGIIDAVVLGQLLGDVVNGVRSE
AELDLYETLRRPAAQEVLDLAGRMTDMALARNPVKRFLRNLALSVVDLSPFLKRRIALKLSGLSRAPLARLPAPSAMPGA
VEASKSETGPKLKLAA
>Mature_416_residues
MLPDMTEILIIGAGPTGMALSIALHQANVDHVLIEKLAHGLQTSRAGVIHAQTLESLEPLGVTQRLSELSVKLEDFAIRD
RNRALLKLDFCKLPSRHPYLMMLPQNLTEQVFAERISALGGTIHREVEAVAVAQDSVGARVTVVQNGQEKIISARYVVGA
DGMQSLVRKSTGIEFDGAAYDGSFVLADVRLDWPVGPTEVSLFFAPAGLVVVAPLPDGSYRVVATMDEAPEKPDVADIQA
LLDSRGPTRNKARVLGIGWSSRFRLHHRLVRAYRKDRLFLMGDAAHVHSPAGGQGMNTGIIDAVVLGQLLGDVVNGVRSE
AELDLYETLRRPAAQEVLDLAGRMTDMALARNPVKRFLRNLALSVVDLSPFLKRRIALKLSGLSRAPLARLPAPSAMPGA
VEASKSETGPKLKLAA

Specific function: Dechlorination of pentachlorophenol to tetrachlorohydroquinone. Removes also hydrogen and nitro, amino, and cyano groups from benzene ring at the para position in relation to the hydroxyl of phenol [H]

COG id: COG0654

COG function: function code HC; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pheA/tfdB FAD monooxygenase family [H]

Homologues:

Organism=Escherichia coli, GI1786543, Length=347, Percent_Identity=25.9365994236311, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI1789273, Length=411, Percent_Identity=24.0875912408759, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI1786883, Length=402, Percent_Identity=26.865671641791, Blast_Score=69, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002938
- InterPro:   IPR003042 [H]

Pfam domain/function: PF01494 FAD_binding_3 [H]

EC number: =1.14.13.50 [H]

Molecular weight: Translated: 44904; Mature: 44904

Theoretical pI: Translated: 9.30; Mature: 9.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLPDMTEILIIGAGPTGMALSIALHQANVDHVLIEKLAHGLQTSRAGVIHAQTLESLEPL
CCCCCEEEEEEECCCCCEEEEEEEECCCCHHHHHHHHHHHHHHCCCCEEEHHHHHHCCCC
GVTQRLSELSVKLEDFAIRDRNRALLKLDFCKLPSRHPYLMMLPQNLTEQVFAERISALG
CHHHHHHHHHHHHHHHEEECCCCEEEEEEECCCCCCCCEEEECCCHHHHHHHHHHHHHHC
GTIHREVEAVAVAQDSVGARVTVVQNGQEKIISARYVVGADGMQSLVRKSTGIEFDGAAY
CHHHHHHHHHEEECCCCCCEEEEEECCCHHHEEEEEEECCHHHHHHHHHHCCCCCCCCEE
DGSFVLADVRLDWPVGPTEVSLFFAPAGLVVVAPLPDGSYRVVATMDEAPEKPDVADIQA
CCCEEEEEEEEECCCCCCEEEEEEECCCEEEEEECCCCCEEEEEEECCCCCCCCHHHHHH
LLDSRGPTRNKARVLGIGWSSRFRLHHRLVRAYRKDRLFLMGDAAHVHSPAGGQGMNTGI
HHHCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCEEEEECHHHCCCCCCCCCCCCHH
IDAVVLGQLLGDVVNGVRSEAELDLYETLRRPAAQEVLDLAGRMTDMALARNPVKRFLRN
HHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LALSVVDLSPFLKRRIALKLSGLSRAPLARLPAPSAMPGAVEASKSETGPKLKLAA
HHHHHHHHHHHHHHHHHHEECCCCCCCHHHCCCCCCCCCCCCCCCCCCCCEEEECC
>Mature Secondary Structure
MLPDMTEILIIGAGPTGMALSIALHQANVDHVLIEKLAHGLQTSRAGVIHAQTLESLEPL
CCCCCEEEEEEECCCCCEEEEEEEECCCCHHHHHHHHHHHHHHCCCCEEEHHHHHHCCCC
GVTQRLSELSVKLEDFAIRDRNRALLKLDFCKLPSRHPYLMMLPQNLTEQVFAERISALG
CHHHHHHHHHHHHHHHEEECCCCEEEEEEECCCCCCCCEEEECCCHHHHHHHHHHHHHHC
GTIHREVEAVAVAQDSVGARVTVVQNGQEKIISARYVVGADGMQSLVRKSTGIEFDGAAY
CHHHHHHHHHEEECCCCCCEEEEEECCCHHHEEEEEEECCHHHHHHHHHHCCCCCCCCEE
DGSFVLADVRLDWPVGPTEVSLFFAPAGLVVVAPLPDGSYRVVATMDEAPEKPDVADIQA
CCCEEEEEEEEECCCCCCEEEEEEECCCEEEEEECCCCCEEEEEEECCCCCCCCHHHHHH
LLDSRGPTRNKARVLGIGWSSRFRLHHRLVRAYRKDRLFLMGDAAHVHSPAGGQGMNTGI
HHHCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCEEEEECHHHCCCCCCCCCCCCHH
IDAVVLGQLLGDVVNGVRSEAELDLYETLRRPAAQEVLDLAGRMTDMALARNPVKRFLRN
HHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LALSVVDLSPFLKRRIALKLSGLSRAPLARLPAPSAMPGAVEASKSETGPKLKLAA
HHHHHHHHHHHHHHHHHHEECCCCCCCHHHCCCCCCCCCCCCCCCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7678243; 12169590 [H]