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The map label for this gene is rpoH [H]

Identifier: 13473215

GI number: 13473215

Start: 3012686

End: 3013597

Strand: Direct

Name: rpoH [H]

Synonym: mlr3741

Alternate gene names: 13473215

Gene position: 3012686-3013597 (Clockwise)

Preceding gene: 13473214

Following gene: 13473220

Centisome position: 42.82

GC content: 63.6

Gene sequence:

>912_bases
ATGGCCCAGTCACTACCTAGTATTGTTTCCGGCGAAGGCGGCCTCAGCCGCTATCTGGAAGAAATCCGCCGCTTTCCCAT
GCTTCAGCCGCAGGAAGAGTACATGCTCGCCAAGCGTTATGCCGAGCATGAAGACACGTCCGCCGCGCACAAGCTCGTCA
CCAGCCATTTGCGGCTCGTCGCCAAGATCGCCATGGGCTATCGCGGCTACGGCCTGCCGATCGGCGAGGTGATCTCGGAA
GGCAATGTCGGCCTGATGCAGGCCGTCAAGAAATTCGAACCCGAGCGCGGCTTCCGCCTCGCGACCTACGCCATGTGGTG
GATCAAGGCCTCGATCCAGGAATACATCCTGCGCTCGTGGAGCCTGGTCAAGATGGGCACGACCGCCAACCAAAAGCGTC
TGTTCTTCAACCTGCGGAAGGTGAAGGGCAAGATCCAGGCGCTGGATGACGGCGATTTGAAGCCCGACCAGATCGCCGAG
ATCGCCACGCGGCTGAACGTTACCGAGGCAGAGGTGGTGTCGATGAACCGCCGCCTGTCAGGCGACGCTTCGCTCAACGC
GCCGATCCGGGCGACGGAAGGCGAATCCGGCGAATGGCAGGATTGGCTGGTCGACGACCACGAAAGCCAGGAAGAGATGC
TGATCGAGCAGGACGAGCTGGAGAATCGGCGCGGCATGCTGTCAGGCGCTCTTGCCGTGCTCAATGAGCGCGAGCGGCGC
ATCTTCGAGGCCCGTCGCCTCGCCGAGGAGCCGCTGACGCTGGAAGAGCTCTCGGCCGAGTTCGACATCAGCCGCGAGCG
CGTGCGCCAGATCGAGGTGCGCGCCTTCGAGAAGGTGCAGGATGCGGTCAAGGCCGCCGCCAAGCGCCAGACCCAGGCGC
TGCGCACCATCGAGGCGCAGCCGGCGGCTTAA

Upstream 100 bases:

>100_bases
TCCTGTATAAGACTGTATGCCGCGAGCGAGCCTTTTGGTGTTCGCGTCACATGCCTGCCGCGTTTCGGGGGCATCACTCC
AATAGAGAGGGGGCGCTATC

Downstream 100 bases:

>100_bases
GCCAGCTTCGGCGACAAAACATCAAGGCGGCGCCAGGATACTGGCGCCGCCTTTTTCGTACGCGGGGTGTCGCGGCGATC
GCCTCAAAGCCCATCCAGCG

Product: RNA polymerase factor sigma-32

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 303; Mature: 302

Protein sequence:

>303_residues
MAQSLPSIVSGEGGLSRYLEEIRRFPMLQPQEEYMLAKRYAEHEDTSAAHKLVTSHLRLVAKIAMGYRGYGLPIGEVISE
GNVGLMQAVKKFEPERGFRLATYAMWWIKASIQEYILRSWSLVKMGTTANQKRLFFNLRKVKGKIQALDDGDLKPDQIAE
IATRLNVTEAEVVSMNRRLSGDASLNAPIRATEGESGEWQDWLVDDHESQEEMLIEQDELENRRGMLSGALAVLNERERR
IFEARRLAEEPLTLEELSAEFDISRERVRQIEVRAFEKVQDAVKAAAKRQTQALRTIEAQPAA

Sequences:

>Translated_303_residues
MAQSLPSIVSGEGGLSRYLEEIRRFPMLQPQEEYMLAKRYAEHEDTSAAHKLVTSHLRLVAKIAMGYRGYGLPIGEVISE
GNVGLMQAVKKFEPERGFRLATYAMWWIKASIQEYILRSWSLVKMGTTANQKRLFFNLRKVKGKIQALDDGDLKPDQIAE
IATRLNVTEAEVVSMNRRLSGDASLNAPIRATEGESGEWQDWLVDDHESQEEMLIEQDELENRRGMLSGALAVLNERERR
IFEARRLAEEPLTLEELSAEFDISRERVRQIEVRAFEKVQDAVKAAAKRQTQALRTIEAQPAA
>Mature_302_residues
AQSLPSIVSGEGGLSRYLEEIRRFPMLQPQEEYMLAKRYAEHEDTSAAHKLVTSHLRLVAKIAMGYRGYGLPIGEVISEG
NVGLMQAVKKFEPERGFRLATYAMWWIKASIQEYILRSWSLVKMGTTANQKRLFFNLRKVKGKIQALDDGDLKPDQIAEI
ATRLNVTEAEVVSMNRRLSGDASLNAPIRATEGESGEWQDWLVDDHESQEEMLIEQDELENRRGMLSGALAVLNERERRI
FEARRLAEEPLTLEELSAEFDISRERVRQIEVRAFEKVQDAVKAAAKRQTQALRTIEAQPAA

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of heat shock promoters [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. Sigma-32 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789871, Length=287, Percent_Identity=38.3275261324042, Blast_Score=184, Evalue=9e-48,
Organism=Escherichia coli, GI1789098, Length=285, Percent_Identity=34.0350877192982, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI1789448, Length=249, Percent_Identity=30.5220883534137, Blast_Score=97, Evalue=2e-21,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012759
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 34461; Mature: 34330

Theoretical pI: Translated: 5.58; Mature: 5.58

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQSLPSIVSGEGGLSRYLEEIRRFPMLQPQEEYMLAKRYAEHEDTSAAHKLVTSHLRLV
CCCCCCHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
AKIAMGYRGYGLPIGEVISEGNVGLMQAVKKFEPERGFRLATYAMWWIKASIQEYILRSW
HHHHHCCCCCCCCHHHHHCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCC
SLVKMGTTANQKRLFFNLRKVKGKIQALDDGDLKPDQIAEIATRLNVTEAEVVSMNRRLS
CCEEECCCCCHHHHHHHHHHHHCHHEECCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHCC
GDASLNAPIRATEGESGEWQDWLVDDHESQEEMLIEQDELENRRGMLSGALAVLNERERR
CCCCCCCCEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IFEARRLAEEPLTLEELSAEFDISRERVRQIEVRAFEKVQDAVKAAAKRQTQALRTIEAQ
HHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PAA
CCC
>Mature Secondary Structure 
AQSLPSIVSGEGGLSRYLEEIRRFPMLQPQEEYMLAKRYAEHEDTSAAHKLVTSHLRLV
CCCCCHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
AKIAMGYRGYGLPIGEVISEGNVGLMQAVKKFEPERGFRLATYAMWWIKASIQEYILRSW
HHHHHCCCCCCCCHHHHHCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCC
SLVKMGTTANQKRLFFNLRKVKGKIQALDDGDLKPDQIAEIATRLNVTEAEVVSMNRRLS
CCEEECCCCCHHHHHHHHHHHHCHHEECCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHCC
GDASLNAPIRATEGESGEWQDWLVDDHESQEEMLIEQDELENRRGMLSGALAVLNERERR
CCCCCCCCEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IFEARRLAEEPLTLEELSAEFDISRERVRQIEVRAFEKVQDAVKAAAKRQTQALRTIEAQ
HHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PAA
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7501460 [H]