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The map label for this gene is yieH [H]

Identifier: 13473121

GI number: 13473121

Start: 2916830

End: 2917504

Strand: Direct

Name: yieH [H]

Synonym: mlr3618

Alternate gene names: 13473121

Gene position: 2916830-2917504 (Clockwise)

Preceding gene: 13473120

Following gene: 13473123

Centisome position: 41.46

GC content: 65.48

Gene sequence:

>675_bases
GTGGCGCGACCAAGGCCTATCATTTTCGATTGCGACGGTGTTCTCGTCGACAGCGAGCCGCTGGCCGCCAGGGCATATGA
GCGCGTCTATGAAAAGCACGGCATGCCGGGCGTCCATGGCGGCATCATCGCCCAATGCGTCGGCATGAAGCAGTCCGACA
TCATCGCCCGGATCAGGGAACTGACCGGCCACCAGTTTCCCGCTGCCGCCGATGGCGATATCTGGGCAGAGACCAAAGTG
CTGTTCACCGAGGAATTGAAGCCGACCCCGGGGATCGCGGCATTTCTGGAAACGCTCGAGGGCGACCGCTGCGTCGCCTC
ATCGTCCTCCGTCGAGCGCATAAACCACAGCCTTGCCGTCACCGGGCTGGCGCATATCTTCGGCGAGGCGATCTACAGCT
CCTCGATGGTCAAGAACGGCAAGCCGGCGCCCGACATCTTCCTGTTCGCCGCCGCCAGGATGGGCGCCGACCCGGCCGAC
TGCATCGTCATTGAGGATTCGCCCTTCGGCATCCAGGGCGCGGTCGCCGCCGGCATGACGGCGATCGGCTACACCGGCGG
CGGTCACACCTATGCCGAGCACGCTGCGCGACTGACGGCGGCCGGCGCCGATTTCGTCTGCGCCGACTGGCACGAAGTTA
GCCGGCAATTGTCCGGGCTCGGCGTGCCGGCCTGA

Upstream 100 bases:

>100_bases
CCGCCATTTCAGCCCCAAATCGTGCCCGAGACATCGCACTATTGAACATCTGACTTTTTGCCGATAGCGATGTGCGGAAT
TGACGTGAGGAGACGAGCGC

Downstream 100 bases:

>100_bases
TCTAGTAGGGACTGGGTAATCTGAGAGCGGCCCATTCGCTGGGCGGAATTGGTGCGCCCACGCGGAAGTTGAAGGATACG
ACCTTCGTCGCATCGGTGTC

Product: putative hydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 224; Mature: 223

Protein sequence:

>224_residues
MARPRPIIFDCDGVLVDSEPLAARAYERVYEKHGMPGVHGGIIAQCVGMKQSDIIARIRELTGHQFPAAADGDIWAETKV
LFTEELKPTPGIAAFLETLEGDRCVASSSSVERINHSLAVTGLAHIFGEAIYSSSMVKNGKPAPDIFLFAAARMGADPAD
CIVIEDSPFGIQGAVAAGMTAIGYTGGGHTYAEHAARLTAAGADFVCADWHEVSRQLSGLGVPA

Sequences:

>Translated_224_residues
MARPRPIIFDCDGVLVDSEPLAARAYERVYEKHGMPGVHGGIIAQCVGMKQSDIIARIRELTGHQFPAAADGDIWAETKV
LFTEELKPTPGIAAFLETLEGDRCVASSSSVERINHSLAVTGLAHIFGEAIYSSSMVKNGKPAPDIFLFAAARMGADPAD
CIVIEDSPFGIQGAVAAGMTAIGYTGGGHTYAEHAARLTAAGADFVCADWHEVSRQLSGLGVPA
>Mature_223_residues
ARPRPIIFDCDGVLVDSEPLAARAYERVYEKHGMPGVHGGIIAQCVGMKQSDIIARIRELTGHQFPAAADGDIWAETKVL
FTEELKPTPGIAAFLETLEGDRCVASSSSVERINHSLAVTGLAHIFGEAIYSSSMVKNGKPAPDIFLFAAARMGADPADC
IVIEDSPFGIQGAVAAGMTAIGYTGGGHTYAEHAARLTAAGADFVCADWHEVSRQLSGLGVPA

Specific function: Catalyzes the dephosphorylation of the artificial chromogenic substrate p-nitrophenyl phosphate (pNPP) and of the natural substrates phosphoenolpyruvate and AMP [H]

COG id: COG0637

COG function: function code R; Predicted phosphatase/phosphohexomutase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family [H]

Homologues:

Organism=Escherichia coli, GI1790151, Length=191, Percent_Identity=28.2722513089005, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1789046, Length=179, Percent_Identity=34.0782122905028, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1787576, Length=195, Percent_Identity=27.1794871794872, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI87082080, Length=205, Percent_Identity=29.7560975609756, Blast_Score=62, Evalue=4e-11,
Organism=Drosophila melanogaster, GI17137324, Length=211, Percent_Identity=32.7014218009479, Blast_Score=84, Evalue=8e-17,
Organism=Drosophila melanogaster, GI116008157, Length=211, Percent_Identity=30.3317535545024, Blast_Score=73, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439
- InterPro:   IPR006402
- InterPro:   IPR005833 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: NA

Molecular weight: Translated: 23567; Mature: 23436

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARPRPIIFDCDGVLVDSEPLAARAYERVYEKHGMPGVHGGIIAQCVGMKQSDIIARIRE
CCCCCCEEEECCCEEECCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCHHHHHHHHHH
LTGHQFPAAADGDIWAETKVLFTEELKPTPGIAAFLETLEGDRCVASSSSVERINHSLAV
HHCCCCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHCCCCEECCCCHHHHHHHHHHH
TGLAHIFGEAIYSSSMVKNGKPAPDIFLFAAARMGADPADCIVIEDSPFGIQGAVAAGMT
HHHHHHHHHHHHHHHHHCCCCCCCHHHEEEHHHCCCCCCCEEEECCCCCCCHHHHHHCHH
AIGYTGGGHTYAEHAARLTAAGADFVCADWHEVSRQLSGLGVPA
HEEECCCCCHHHHHHHHHHHCCCCEEECCHHHHHHHHHCCCCCC
>Mature Secondary Structure 
ARPRPIIFDCDGVLVDSEPLAARAYERVYEKHGMPGVHGGIIAQCVGMKQSDIIARIRE
CCCCCEEEECCCEEECCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCHHHHHHHHHH
LTGHQFPAAADGDIWAETKVLFTEELKPTPGIAAFLETLEGDRCVASSSSVERINHSLAV
HHCCCCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHCCCCEECCCCHHHHHHHHHHH
TGLAHIFGEAIYSSSMVKNGKPAPDIFLFAAARMGADPADCIVIEDSPFGIQGAVAAGMT
HHHHHHHHHHHHHHHHHCCCCCCCHHHEEEHHHCCCCCCCEEEECCCCCCCHHHHHHCHH
AIGYTGGGHTYAEHAARLTAAGADFVCADWHEVSRQLSGLGVPA
HEEECCCCCHHHHHHHHHHHCCCCEEECCHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7686882; 9278503 [H]