Click here to switch to the map view.

The map label for this gene is gluQ [H]

Identifier: 13473005

GI number: 13473005

Start: 2805981

End: 2806856

Strand: Direct

Name: gluQ [H]

Synonym: mlr3479

Alternate gene names: 13473005

Gene position: 2805981-2806856 (Clockwise)

Preceding gene: 13473002

Following gene: 13473006

Centisome position: 39.88

GC content: 65.53

Gene sequence:

>876_bases
ATGACGCTCCTGACATTCCGCTTCGCCCCCAGCCCCAACGGCGAACTGCATCTCGGCCACGCCTATTCGGCGCTGCTCAA
CCAGAAGCTCGCAGAGGCCCATGGCGGCCGCCTGCTGCTGCGCATCGAGGACATCGACACCACGCGCTGCACGCCGCAAT
TCGAGGCCGGCGTACTTGCCGACATGAAATGGCTGGGTTTTGGCTGGGAGCAGCCCGTGCGCCGCCAGTCCGAGCATTTC
GTCGAGTATCAGGCGGTGCTCGACCGGCTGATCCGCGAGGAGCTCGTTTATCCCGCCTTCATGAGCCGAGGCGACATCCG
TGCCTTCATCGCCGACAGCGAAAAGCGCGGGCGTGACTGGCCGCGCGATCCCGATGGCGTGCCGTTCTATCCTCCGTCCG
ACAAGGCGTTGCCGGCGAAAGAACGCAAACGGCGCATCGCTGAAAACGAGCCTTTCGCCTGGCGGCTGAATGTCGATGCC
GCTGTTGCGCGTGCCGGCAGGGACCTCGTATGGACCGAATTCACCGATGAGTCGCTATCGGCGACGCGCTCGGTCGAGGC
CCGCCCACAGGACTGGGGCGACGTGATCGTGGCGCGCCGCGACATCCCGACCAGCTACCACCTCGCCGTCATTGTCGATG
ACGCCCTGCAAGGGGTCAGCCATGTCGTGCGTGGGCAGGACCTGTTTTCCGCCACCGGCGTGCAGCGCCTGCTACAGGAA
TTGCTCGGGCTGCCGCAACCGGCCTATTTCCACCATCGGCTCATCCTCGGGCCGGACGGGCGAAAACTGTCGAAGAGCCT
TGGCGACACCGGCCTTGCCAGGCTGCGCGAGGCCGGCGCGTCACCTGACGATATCAGGCGGATGGTCGGTCTCTGA

Upstream 100 bases:

>100_bases
CGTCCAGTGTCGCGATTCGTTGCATGGTAGCCTTGCCCGCCAATGTGCAGCAAAGTAGCAACCGCCAAAACCGCAGCACA
ACCCGATTGCCGCTCGAGAC

Downstream 100 bases:

>100_bases
GTGTGGCGAAGAGCTCTGGCAAACGCGCGCCGCACGCACCGCCGATTGAATTCCCATGATGCATGGATCAATCGTTTCGT
TTTGCACCCCGGGTTGAACC

Product: glutamyl-Q tRNA(Asp) synthetase

Products: AMP; L-Glutamyl-tRNA(Glu); Diphosphate [C]

Alternate protein names: Glu-Q-RSs [H]

Number of amino acids: Translated: 291; Mature: 290

Protein sequence:

>291_residues
MTLLTFRFAPSPNGELHLGHAYSALLNQKLAEAHGGRLLLRIEDIDTTRCTPQFEAGVLADMKWLGFGWEQPVRRQSEHF
VEYQAVLDRLIREELVYPAFMSRGDIRAFIADSEKRGRDWPRDPDGVPFYPPSDKALPAKERKRRIAENEPFAWRLNVDA
AVARAGRDLVWTEFTDESLSATRSVEARPQDWGDVIVARRDIPTSYHLAVIVDDALQGVSHVVRGQDLFSATGVQRLLQE
LLGLPQPAYFHHRLILGPDGRKLSKSLGDTGLARLREAGASPDDIRRMVGL

Sequences:

>Translated_291_residues
MTLLTFRFAPSPNGELHLGHAYSALLNQKLAEAHGGRLLLRIEDIDTTRCTPQFEAGVLADMKWLGFGWEQPVRRQSEHF
VEYQAVLDRLIREELVYPAFMSRGDIRAFIADSEKRGRDWPRDPDGVPFYPPSDKALPAKERKRRIAENEPFAWRLNVDA
AVARAGRDLVWTEFTDESLSATRSVEARPQDWGDVIVARRDIPTSYHLAVIVDDALQGVSHVVRGQDLFSATGVQRLLQE
LLGLPQPAYFHHRLILGPDGRKLSKSLGDTGLARLREAGASPDDIRRMVGL
>Mature_290_residues
TLLTFRFAPSPNGELHLGHAYSALLNQKLAEAHGGRLLLRIEDIDTTRCTPQFEAGVLADMKWLGFGWEQPVRRQSEHFV
EYQAVLDRLIREELVYPAFMSRGDIRAFIADSEKRGRDWPRDPDGVPFYPPSDKALPAKERKRRIAENEPFAWRLNVDAA
VARAGRDLVWTEFTDESLSATRSVEARPQDWGDVIVARRDIPTSYHLAVIVDDALQGVSHVVRGQDLFSATGVQRLLQEL
LGLPQPAYFHHRLILGPDGRKLSKSLGDTGLARLREAGASPDDIRRMVGL

Specific function: Catalyzes the tRNA-independent activation of glutamate in presence of ATP and the subsequent transfer of glutamate onto a tRNA(Asp). Glutamate is transferred on the 2-amino-5-(4,5- dihydroxy-2-cyclopenten-1-yl) moiety of the queuosine in the wobble positi

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family. GluQ subfamily [H]

Homologues:

Organism=Homo sapiens, GI134288884, Length=291, Percent_Identity=30.5841924398625, Blast_Score=115, Evalue=5e-26,
Organism=Escherichia coli, GI1788743, Length=277, Percent_Identity=31.0469314079422, Blast_Score=130, Evalue=8e-32,
Organism=Escherichia coli, GI226510925, Length=259, Percent_Identity=33.2046332046332, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1786895, Length=120, Percent_Identity=32.5, Blast_Score=75, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI71993394, Length=292, Percent_Identity=26.7123287671233, Blast_Score=100, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6324540, Length=285, Percent_Identity=28.0701754385965, Blast_Score=119, Evalue=5e-28,
Organism=Saccharomyces cerevisiae, GI6321192, Length=97, Percent_Identity=32.9896907216495, Blast_Score=67, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24665321, Length=291, Percent_Identity=29.8969072164948, Blast_Score=111, Evalue=4e-25,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR022380
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c [H]

EC number: 6.1.1.17 [C]

Molecular weight: Translated: 32769; Mature: 32637

Theoretical pI: Translated: 6.97; Mature: 6.97

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLLTFRFAPSPNGELHLGHAYSALLNQKLAEAHGGRLLLRIEDIDTTRCTPQFEAGVLA
CEEEEEEECCCCCCCEEEHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCHHH
DMKWLGFGWEQPVRRQSEHFVEYQAVLDRLIREELVYPAFMSRGDIRAFIADSEKRGRDW
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHCCCC
PRDPDGVPFYPPSDKALPAKERKRRIAENEPFAWRLNVDAAVARAGRDLVWTEFTDESLS
CCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEECCHHHHHHCCCCEEEEECCCHHHH
ATRSVEARPQDWGDVIVARRDIPTSYHLAVIVDDALQGVSHVVRGQDLFSATGVQRLLQE
HHHCCCCCCCCCCCEEEEECCCCCCEEEEEEEHHHHHHHHHHHHCCHHHHHHHHHHHHHH
LLGLPQPAYFHHRLILGPDGRKLSKSLGDTGLARLREAGASPDDIRRMVGL
HHCCCCCCHHCEEEEECCCCHHHHHHHCHHHHHHHHHCCCCHHHHHHHHCC
>Mature Secondary Structure 
TLLTFRFAPSPNGELHLGHAYSALLNQKLAEAHGGRLLLRIEDIDTTRCTPQFEAGVLA
EEEEEEECCCCCCCEEEHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCHHH
DMKWLGFGWEQPVRRQSEHFVEYQAVLDRLIREELVYPAFMSRGDIRAFIADSEKRGRDW
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHCCCC
PRDPDGVPFYPPSDKALPAKERKRRIAENEPFAWRLNVDAAVARAGRDLVWTEFTDESLS
CCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEECCHHHHHHCCCCEEEEECCCHHHH
ATRSVEARPQDWGDVIVARRDIPTSYHLAVIVDDALQGVSHVVRGQDLFSATGVQRLLQE
HHHCCCCCCCCCCCEEEEECCCCCCEEEEEEEHHHHHHHHHHHHCCHHHHHHHHHHHHHH
LLGLPQPAYFHHRLILGPDGRKLSKSLGDTGLARLREAGASPDDIRRMVGL
HHCCCCCCHHCEEEEECCCCHHHHHHHCHHHHHHHHHCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-Glutamate; tRNA (Glu) [C]

Specific reaction: ATP + L-Glutamate + tRNA (Glu) --> AMP + L-Glutamyl-tRNA(Glu) + Diphosphate [C]

General reaction: Aminoacylation; Esterification [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA