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The map label for this gene is stp [H]

Identifier: 13472962

GI number: 13472962

Start: 2761767

End: 2763341

Strand: Reverse

Name: stp [H]

Synonym: mll3424

Alternate gene names: 13472962

Gene position: 2763341-2761767 (Counterclockwise)

Preceding gene: 13472963

Following gene: 13472960

Centisome position: 39.27

GC content: 64.44

Gene sequence:

>1575_bases
ATGCATTCGCCAAATGAAGCCGCCGCAATCCCGCTGACCAGCCGGCTCACGAACGGAACCTTTTGTCCGCAATCGCAGCG
GCGATTCGTCTTGATCGCGGCGATCCTGGCCTCGGCGCTCGGCTTCATCGACGGGTCGATCCTGGCCATCGCCATGCCGG
CGATCCGTGTCAACCTCGGCGCCAGCCTTGCCGAGGCGCAGTGGATCTCCAATGCCTATGCGTTGACACTTTCGGCGCTG
ATCCTTGCCGGCGGCGCCGCCGGCGACCGGTTCGGGCTGCGCCGTGCCTTCGTGGCCGGCATCGCGCTGTTCATTGTTGC
CTCGCTCGCCTGCGCATTGGCGCCGAATGCTGTCGTGCTGATCGGTTTTCGCGCTCTCCAAGGAATTGGGGCGGCGATCA
TGGTGCCGGGCAGCCTCGCCATCATTGCCAAGGCCTATCCGAAAAAGGAGCGCGGCCGTGCGATCGGCATCTGGGCGGCA
GCCTCGGCGCTGACCACGGCCCTTGGGCCGGTGCTCGGTGGTTTGGTGCTGTCGGTTTTTGGCGATGGCATCTGGCGGGC
AATCTTCGCCGTCAACCTGCCGCTCGGCCTGATCTCGATCTACCTGCTGCTCGTCAAGATTCCGGCCGATGCGGCGACGG
AAAAACGCAGCCTCGACCTCGGCGGTGGCGCGCTCGCGACATTGGCGTTCGGCGCACTCGCCTACGGGCTGACCTCGATG
AGTTCGAGCAGCGAGGGCCATATGGCCGGGCCGAGCATTGCCGCCGGCGCGGTGCTGCTTGTCGTCTTCATCCTGTTCGA
GCAGCGCCAGCGCGAGCCGATGATCGACCTCAGCCTGTTTCGCATCGGCGCCTTCGCCGGTGCCAATGTGGCGACCTTCT
TTCTGTATTTCGCGCTGTCGGCCAATCTTTTCTATCTGCCGATGCTGCTGATCGCCGGCTGGGGACTGAGCACGGCCGAG
GTCGGCTTCATCTTCCTGCCGCTGTCGACATCGATCGCGCTTCTGTCGGGACCGGCCGGCCAGTTGTCGGACCGGATCGG
ACCGCGTTTCCCGATTGCCTGCGGCAGCCTGATCGTGGCTTTCGCTTTCGCCGGACTTGCCTTGCTCAGCCATGCCGGCA
TCCACCATTTCTGGACTGGGATACTTCCGCTGATGGCGCTGATGGGCCTCGGCATGGCGCTGGTCGTCTCACCACTGTCG
ACCGCGATCATGACGGCGGTGGAAGACAAGGACACGGGGGCTGCCTCCGGCATCAACAATGCCGTCTCGCGCATTGGCGG
CCTGATCGCGGTGGCGGCGATGGGCTCGCTTGCCACGTGGGTCTATGCAACGATGCTCGACACCAGTGTCCGGCCGGGCA
TTCCGGGTTTTGGCGAACCGGTATCGACAGGCTTGCCGCCAGAACTTGAAGCAACACGGCTTGCTGCCAGCGACATGGCT
TTTTCAGCCATATCCTTGACGACCGCCGTGCTCTGCCTGCTTGCCGCGATCATCGCCTGGGTGACCGTTTCGGGTCAGGC
ACTGCCCTGGCCGCGCCGCACGGACCAATCGTGGACTGATTCGTCGCGAAGCTGA

Upstream 100 bases:

>100_bases
TGCATCGGCACGGGACGCATCCTGGCCAATTGGCTGGTTTCAAGGACTGCAAGCTGCTGGCGGATTGTTGCCGGCGCGCT
TGGGGTCTAAGCAAGGTGCC

Downstream 100 bases:

>100_bases
TTTGCTCTTGCCGGATCGGCCGAGGTTCAGCCGTCGATCTTGGCGCTGGCGACCTTCAGCGAGTTGCCATCTTCCTGCTT
CAGCAGATCGTCGATGCGCT

Product: transmembrane efflux protein

Products: NA

Alternate protein names: Spectinomycin tetracycline efflux pump [H]

Number of amino acids: Translated: 524; Mature: 524

Protein sequence:

>524_residues
MHSPNEAAAIPLTSRLTNGTFCPQSQRRFVLIAAILASALGFIDGSILAIAMPAIRVNLGASLAEAQWISNAYALTLSAL
ILAGGAAGDRFGLRRAFVAGIALFIVASLACALAPNAVVLIGFRALQGIGAAIMVPGSLAIIAKAYPKKERGRAIGIWAA
ASALTTALGPVLGGLVLSVFGDGIWRAIFAVNLPLGLISIYLLLVKIPADAATEKRSLDLGGGALATLAFGALAYGLTSM
SSSSEGHMAGPSIAAGAVLLVVFILFEQRQREPMIDLSLFRIGAFAGANVATFFLYFALSANLFYLPMLLIAGWGLSTAE
VGFIFLPLSTSIALLSGPAGQLSDRIGPRFPIACGSLIVAFAFAGLALLSHAGIHHFWTGILPLMALMGLGMALVVSPLS
TAIMTAVEDKDTGAASGINNAVSRIGGLIAVAAMGSLATWVYATMLDTSVRPGIPGFGEPVSTGLPPELEATRLAASDMA
FSAISLTTAVLCLLAAIIAWVTVSGQALPWPRRTDQSWTDSSRS

Sequences:

>Translated_524_residues
MHSPNEAAAIPLTSRLTNGTFCPQSQRRFVLIAAILASALGFIDGSILAIAMPAIRVNLGASLAEAQWISNAYALTLSAL
ILAGGAAGDRFGLRRAFVAGIALFIVASLACALAPNAVVLIGFRALQGIGAAIMVPGSLAIIAKAYPKKERGRAIGIWAA
ASALTTALGPVLGGLVLSVFGDGIWRAIFAVNLPLGLISIYLLLVKIPADAATEKRSLDLGGGALATLAFGALAYGLTSM
SSSSEGHMAGPSIAAGAVLLVVFILFEQRQREPMIDLSLFRIGAFAGANVATFFLYFALSANLFYLPMLLIAGWGLSTAE
VGFIFLPLSTSIALLSGPAGQLSDRIGPRFPIACGSLIVAFAFAGLALLSHAGIHHFWTGILPLMALMGLGMALVVSPLS
TAIMTAVEDKDTGAASGINNAVSRIGGLIAVAAMGSLATWVYATMLDTSVRPGIPGFGEPVSTGLPPELEATRLAASDMA
FSAISLTTAVLCLLAAIIAWVTVSGQALPWPRRTDQSWTDSSRS
>Mature_524_residues
MHSPNEAAAIPLTSRLTNGTFCPQSQRRFVLIAAILASALGFIDGSILAIAMPAIRVNLGASLAEAQWISNAYALTLSAL
ILAGGAAGDRFGLRRAFVAGIALFIVASLACALAPNAVVLIGFRALQGIGAAIMVPGSLAIIAKAYPKKERGRAIGIWAA
ASALTTALGPVLGGLVLSVFGDGIWRAIFAVNLPLGLISIYLLLVKIPADAATEKRSLDLGGGALATLAFGALAYGLTSM
SSSSEGHMAGPSIAAGAVLLVVFILFEQRQREPMIDLSLFRIGAFAGANVATFFLYFALSANLFYLPMLLIAGWGLSTAE
VGFIFLPLSTSIALLSGPAGQLSDRIGPRFPIACGSLIVAFAFAGLALLSHAGIHHFWTGILPLMALMGLGMALVVSPLS
TAIMTAVEDKDTGAASGINNAVSRIGGLIAVAAMGSLATWVYATMLDTSVRPGIPGFGEPVSTGLPPELEATRLAASDMA
FSAISLTTAVLCLLAAIIAWVTVSGQALPWPRRTDQSWTDSSRS

Specific function: Contributes to spectinomycin and tetracycline resistance [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI87081983, Length=402, Percent_Identity=29.6019900497512, Blast_Score=149, Evalue=5e-37,
Organism=Escherichia coli, GI1790195, Length=385, Percent_Identity=29.6103896103896, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI1788710, Length=406, Percent_Identity=26.8472906403941, Blast_Score=122, Evalue=5e-29,
Organism=Escherichia coli, GI1789042, Length=439, Percent_Identity=25.0569476082005, Blast_Score=109, Evalue=5e-25,
Organism=Escherichia coli, GI1788392, Length=404, Percent_Identity=27.4752475247525, Blast_Score=89, Evalue=7e-19,
Organism=Escherichia coli, GI1790146, Length=171, Percent_Identity=28.6549707602339, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 53891; Mature: 53891

Theoretical pI: Translated: 8.90; Mature: 8.90

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHSPNEAAAIPLTSRLTNGTFCPQSQRRFVLIAAILASALGFIDGSILAIAMPAIRVNLG
CCCCCCCCCCCHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHEECC
ASLAEAQWISNAYALTLSALILAGGAAGDRFGLRRAFVAGIALFIVASLACALAPNAVVL
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEE
IGFRALQGIGAAIMVPGSLAIIAKAYPKKERGRAIGIWAAASALTTALGPVLGGLVLSVF
EEHHHHHCCCEEEECCCCCEEHHHHCCCHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHH
GDGIWRAIFAVNLPLGLISIYLLLVKIPADAATEKRSLDLGGGALATLAFGALAYGLTSM
HHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHC
SSSSEGHMAGPSIAAGAVLLVVFILFEQRQREPMIDLSLFRIGAFAGANVATFFLYFALS
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHH
ANLFYLPMLLIAGWGLSTAEVGFIFLPLSTSIALLSGPAGQLSDRIGPRFPIACGSLIVA
CCHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCEECCCCHHHHHHCCCCCCHHHHHHHHH
FAFAGLALLSHAGIHHFWTGILPLMALMGLGMALVVSPLSTAIMTAVEDKDTGAASGINN
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHH
AVSRIGGLIAVAAMGSLATWVYATMLDTSVRPGIPGFGEPVSTGLPPELEATRLAASDMA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
FSAISLTTAVLCLLAAIIAWVTVSGQALPWPRRTDQSWTDSSRS
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MHSPNEAAAIPLTSRLTNGTFCPQSQRRFVLIAAILASALGFIDGSILAIAMPAIRVNLG
CCCCCCCCCCCHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHEECC
ASLAEAQWISNAYALTLSALILAGGAAGDRFGLRRAFVAGIALFIVASLACALAPNAVVL
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEE
IGFRALQGIGAAIMVPGSLAIIAKAYPKKERGRAIGIWAAASALTTALGPVLGGLVLSVF
EEHHHHHCCCEEEECCCCCEEHHHHCCCHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHH
GDGIWRAIFAVNLPLGLISIYLLLVKIPADAATEKRSLDLGGGALATLAFGALAYGLTSM
HHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHC
SSSSEGHMAGPSIAAGAVLLVVFILFEQRQREPMIDLSLFRIGAFAGANVATFFLYFALS
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHH
ANLFYLPMLLIAGWGLSTAEVGFIFLPLSTSIALLSGPAGQLSDRIGPRFPIACGSLIVA
CCHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCEECCCCHHHHHHCCCCCCHHHHHHHHH
FAFAGLALLSHAGIHHFWTGILPLMALMGLGMALVVSPLSTAIMTAVEDKDTGAASGINN
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHH
AVSRIGGLIAVAAMGSLATWVYATMLDTSVRPGIPGFGEPVSTGLPPELEATRLAASDMA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
FSAISLTTAVLCLLAAIIAWVTVSGQALPWPRRTDQSWTDSSRS
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]