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The map label for this gene is alkR [H]

Identifier: 13472926

GI number: 13472926

Start: 2712912

End: 2713898

Strand: Direct

Name: alkR [H]

Synonym: mlr3375

Alternate gene names: 13472926

Gene position: 2712912-2713898 (Clockwise)

Preceding gene: 13472924

Following gene: 13472927

Centisome position: 38.56

GC content: 65.25

Gene sequence:

>987_bases
ATGCTTGACCATTCGTCCAAAATTCCAATCACGGGCGACCCGCTGACGGACATATTGCGCGGCCTGCGCCTCGATGGCGT
CGAGTATGGCCGCTGCGAGATGAAAGAGCCTTGGGGCATCCAGTTTCCGGCTCAGCCAGGGGCGCGGTTTCACTTCATCG
GCCGCAAGGGCTGCTGGCTGAAGCAGCCCTCCGGCGAATGGGTTGAGCTCAATGCCGGCGATGCGGTGCTGCTGCCGCGC
GGCGAGGCCCATGTACTGGCCAGCGAACCCGGGGCGAAAGCCTGGCCGATCCATGGCTACGAGCTCGAGGAAGTCTGCAA
GGACGTCTACTGCACGTCGAACGCGGAGATGTGCGACAAGAATTGCCAGCCGGGAACGCTGCTGTTCTGCGCCGCGATGT
ATTTCAACATCGACGGCCTGCATCCGCTGCTCGGCATGATGCCGGACGTGATGCGCTCGCATGAGCTGGAAGCCCATGAG
CCGGGCATCCCGCACCTGCTGGAATCTATGGCGCGCGAAGTGACGATGGAGCGCGTCGGCTCCGGCGGCATCCTGGCGCG
GCTGGCCGATGTGGTCGCCGCCACCGTCATCCGCTCATGGGTCGAGCGTGGCTGCGGCGATTCCTCCGGCTGGATCGCTG
CGGTCCGGGACCGTGACATCGGCCGCGTGCTGGCCGCCATCCATCTCGAACCGGACCACGACTGGACGGTCGAGTCGCTG
GCCAGGATGATGGGCGCCTCACGTTCGGGCTTTGCCGAGCGCTTTGCCACCATTGTCGGCGAAACGCCGGCAAAATATGT
CACCCAGGTCAGGATGCATCTGGCGCGGCAATGGCTGGTCCGCGACCGCATGAAGATCTCGGTCGTTGCCGCCCGGCTCG
GCTATGAATCGGACGCCGCCTTCAGCCGTGCCTTCAAGCGCGTCATCGGTTCGGCACCCAGCCATCTGCGCGCCGAGGAG
CAGGCTGACATCCGCCAGGCCGGGTAG

Upstream 100 bases:

>100_bases
CTTGGCCATGGCCGGTCTGCGATTTGTTTGCGGCGCAACATTTGGCTGCAGTGCAACATAGGAAGTGACGCCCTGGAGTT
TCCATGCTAGAAAACCCATT

Downstream 100 bases:

>100_bases
CGTATCTCGGCCGTGACGGACTGGCGATTCCACGCTAGAACCCGGGCATGGATTTCACATCATCCGAGAGTGTTTCGCCG
GCTTCAGGCGGTCCGCTGAC

Product: transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 328; Mature: 328

Protein sequence:

>328_residues
MLDHSSKIPITGDPLTDILRGLRLDGVEYGRCEMKEPWGIQFPAQPGARFHFIGRKGCWLKQPSGEWVELNAGDAVLLPR
GEAHVLASEPGAKAWPIHGYELEEVCKDVYCTSNAEMCDKNCQPGTLLFCAAMYFNIDGLHPLLGMMPDVMRSHELEAHE
PGIPHLLESMAREVTMERVGSGGILARLADVVAATVIRSWVERGCGDSSGWIAAVRDRDIGRVLAAIHLEPDHDWTVESL
ARMMGASRSGFAERFATIVGETPAKYVTQVRMHLARQWLVRDRMKISVVAARLGYESDAAFSRAFKRVIGSAPSHLRAEE
QADIRQAG

Sequences:

>Translated_328_residues
MLDHSSKIPITGDPLTDILRGLRLDGVEYGRCEMKEPWGIQFPAQPGARFHFIGRKGCWLKQPSGEWVELNAGDAVLLPR
GEAHVLASEPGAKAWPIHGYELEEVCKDVYCTSNAEMCDKNCQPGTLLFCAAMYFNIDGLHPLLGMMPDVMRSHELEAHE
PGIPHLLESMAREVTMERVGSGGILARLADVVAATVIRSWVERGCGDSSGWIAAVRDRDIGRVLAAIHLEPDHDWTVESL
ARMMGASRSGFAERFATIVGETPAKYVTQVRMHLARQWLVRDRMKISVVAARLGYESDAAFSRAFKRVIGSAPSHLRAEE
QADIRQAG
>Mature_328_residues
MLDHSSKIPITGDPLTDILRGLRLDGVEYGRCEMKEPWGIQFPAQPGARFHFIGRKGCWLKQPSGEWVELNAGDAVLLPR
GEAHVLASEPGAKAWPIHGYELEEVCKDVYCTSNAEMCDKNCQPGTLLFCAAMYFNIDGLHPLLGMMPDVMRSHELEAHE
PGIPHLLESMAREVTMERVGSGGILARLADVVAATVIRSWVERGCGDSSGWIAAVRDRDIGRVLAAIHLEPDHDWTVESL
ARMMGASRSGFAERFATIVGETPAKYVTQVRMHLARQWLVRDRMKISVVAARLGYESDAAFSRAFKRVIGSAPSHLRAEE
QADIRQAG

Specific function: This protein activates the expression of the alkane 1- monooxygenase AlkM [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786496, Length=309, Percent_Identity=25.5663430420712, Blast_Score=91, Evalue=8e-20,
Organism=Escherichia coli, GI87082197, Length=134, Percent_Identity=35.0746268656716, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR020449
- InterPro:   IPR018060 [H]

Pfam domain/function: PF00165 HTH_AraC [H]

EC number: NA

Molecular weight: Translated: 36227; Mature: 36227

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00041 HTH_ARAC_FAMILY_1 ; PS01124 HTH_ARAC_FAMILY_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDHSSKIPITGDPLTDILRGLRLDGVEYGRCEMKEPWGIQFPAQPGARFHFIGRKGCWL
CCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCEE
KQPSGEWVELNAGDAVLLPRGEAHVLASEPGAKAWPIHGYELEEVCKDVYCTSNAEMCDK
ECCCCCEEEECCCCEEEECCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHC
NCQPGTLLFCAAMYFNIDGLHPLLGMMPDVMRSHELEAHEPGIPHLLESMAREVTMERVG
CCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCC
SGGILARLADVVAATVIRSWVERGCGDSSGWIAAVRDRDIGRVLAAIHLEPDHDWTVESL
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCHHHEEEHEEECCCCCCCHHHH
ARMMGASRSGFAERFATIVGETPAKYVTQVRMHLARQWLVRDRMKISVVAARLGYESDAA
HHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
FSRAFKRVIGSAPSHLRAEEQADIRQAG
HHHHHHHHHCCCCHHHCCHHHHHHHCCC
>Mature Secondary Structure
MLDHSSKIPITGDPLTDILRGLRLDGVEYGRCEMKEPWGIQFPAQPGARFHFIGRKGCWL
CCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCEE
KQPSGEWVELNAGDAVLLPRGEAHVLASEPGAKAWPIHGYELEEVCKDVYCTSNAEMCDK
ECCCCCEEEECCCCEEEECCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHC
NCQPGTLLFCAAMYFNIDGLHPLLGMMPDVMRSHELEAHEPGIPHLLESMAREVTMERVG
CCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCC
SGGILARLADVVAATVIRSWVERGCGDSSGWIAAVRDRDIGRVLAAIHLEPDHDWTVESL
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCHHHEEEHEEECCCCCCCHHHH
ARMMGASRSGFAERFATIVGETPAKYVTQVRMHLARQWLVRDRMKISVVAARLGYESDAA
HHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
FSRAFKRVIGSAPSHLRAEEQADIRQAG
HHHHHHHHHCCCCHHHCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9546151 [H]