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The map label for this gene is gacS [H]

Identifier: 13472804

GI number: 13472804

Start: 2569581

End: 2571863

Strand: Direct

Name: gacS [H]

Synonym: mlr3215

Alternate gene names: 13472804

Gene position: 2569581-2571863 (Clockwise)

Preceding gene: 13472803

Following gene: 13472805

Centisome position: 36.52

GC content: 67.02

Gene sequence:

>2283_bases
ATGATCGCGGAATCCGACAGCCAACAGGCAAACAAGGGCGACGATGCGGTTGCCGCGCCGGCGGCGGCGCGGCGGTTCAT
CGCTGCGCCGCCGCTGGTGCCCGAGCAGCAATTGGCCAGGCGGGAAGGCCTGCCGCTGCTCACCTTCGTTGCCATCATGG
TCCTGGCGGGCCTCGCGCATCTGACCGGGGCGCCGATCTTCATCAGCCTCGCCTTGCTGGCAACCGGCCTCGCCGGCCTT
GCCTTGCATCTCCATGCCAGACGCAGCGTCCACCGTACCGTGGTGCTGCTGGACGAGACCACCGCCCGAAGCCGCGCCGA
GATCGAGACGCTGGCCGACCGCATGTGGGAAATGCAGGAAAGCGAGGAGCGCTTTCGCGGTCTCATCGATGCGCTGGGCG
ATCTCGTCATCCATCGCGATCGCGACGGCTACATCGTCTATGCCAACAAGGTGTTCGCCGATCTCGTCGAAACCGATCAG
CGCGATCTTGCCGGCAAGACCTTGGCCGAACTCGGCATCGAGGTCGGCATCGTCCCGGACGCCGCCTTTTCCGACCATGA
GTGCCTGAGTTCCACCGATGTCGCCATCCGCACGCCGGGCGGCCCGCGCTGGTTCTCCTGGATCGAACTGTCGGTGCGCG
ACAAGGACAGCGGCGCGGCATCGCATCGCGCCATCGCCCGCGACATCACCGCCCGCAAGCGCGCGGAATCCTCGCTGATC
ACCGCGCGCGAGCGCGCCGAATACGCAAGCCAGGCCAAATCGCGCTTCCTCGCCACGGTCAGCCATGAAATTCGCACGCC
GATGAACGGCATCATGGGCATGGCCAGGCTGCTCGCCGATACCAGCCTGTCGCCGGAACAGCAGACCTATGTCGGCGCCA
TATCGACCTCGGCCAGCGCCCTGCTCGCCCTGATCGAGGACCTGCTCGACTATTCCAAGATCGAGGCGGGTCGCTTCGAT
CCGGAACCGCAGCCCATGTCGGTTCGCGAGATCGCCGACAACATCATCGAATTGCTCGCCGCGCGCGCCTTCGCCAAGGA
TATCGGTCTCGGCTGCCACGTCGAACCGGATGTACCGCAATTGATCACGGCCGACCCTGGCCGGGTCAGGCAGGTGCTGC
TCAATCTCATCGGCAACGCCATCAAGTTCACCGACGGCGGCGGCGTGCTGGTCAGCATCGCGCGCGCCCGGAGCGAGACC
AGCGACCGCATCTGCTTCACCATCACCGACACCGGTCCCGGCCTGCGCGAAGAGGACATGGAGCGCATCTTCGAGGAGTT
CGAACAGGCCGACGGCACCTCGACGCGCGCACATGGCGGGGCGGGGCTCGGACTTGCCATCTCCAAGCGCCTGGTGGCTG
CCATGGGCGGCACGATTTCGGTCTCCAGCCGGCTTGGCCAAGGGTCGGAATTCGTCTTCGAAATCCCGGCCACGGAGGCC
ACCGAGGCGCCCCAGGGCCGGCAGAACGTGCTTGCCGGCAGGCGCGCGGTGATCCTGTCCAGGAACGCCGTCGAGGCCGA
CGCCATCGCCCGCACCATCCGCGCCAATGGCGGCACGGCCGGCATAGCCACCACCGTGGCGCAGGCCGCGCCGCTGGCCG
AGGGCTGCGACGTGCTGCTGGTCGACGCGGCCATGGAGGAGAGTGACGGGAGAGTGCTCAAGCGATTGCGCCAGAGTGGT
TTCACCGATTGCGAAGCCATCACCCTGATCGCACCGACCGACCGAGGCATGCTTGGTGAGTTCCGCGCCAGCGGCTACGC
GACCTTCCTGGCCCGGCCGGTGCGCGGTGGAACACTTTTGCGTGTTCTTTTGACCAGCCACGCGCCGGCCCTTGCCCAGC
CGCAGCCGGAAAAGCGCCGCACCCCGGCACTTCGCGCCTCAAGCGACCGCCAGCAGGGCCTGTCCGTGCTGATCGCCGAA
GACAACGACATCAATGCCATGCTGGCCCGCGCCACGCTGTTGAAGGCGGGGCACCGCGTTAAGGTCGTCGGCAACGGCAA
AGCCGCCGTCGAGGCCGTCACCGGCGCCGGGCACAAGCACCGCTTCGACGTGGTGCTGATGGACCTGCATATGCCTGTTA
TGGACGGGCTGGACGCCATTGCCGCGATCCGCCGCCATGAGGAGGAGACATCGGCGCCGCCCGTGCCGATCATGGTGCTG
TCGGCCGATAGCCAGGAAAAGACGCGCCATGCGGTGCTTGCCCACGGCGCCAGCGGCTTCGTCACCAAGCCACTCGACCC
GGATGCGCTTGTCAACGCCGTCGAGGGCCAGGTTGCAGCTTGA

Upstream 100 bases:

>100_bases
CGGTGGCGAATCCGCTGCTGTCGGGACAAAAACGCCTATCCGCTAAAATTCGAACGATCCGCCGCAATTTTCCATAATCT
TCCTCTGGCAAGGGTTTCGC

Downstream 100 bases:

>100_bases
TCTGCTTTTTTCCAGCCGCGCCTCTGACGCATGCCGGCTCAAAGCCGCCCCGATTCGCCGAGGGCGACATCCATGAAAAC
AAGGGTTTGGGGCGCAGTGA

Product: two-component sensor/response regulator hybrid

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 760; Mature: 760

Protein sequence:

>760_residues
MIAESDSQQANKGDDAVAAPAAARRFIAAPPLVPEQQLARREGLPLLTFVAIMVLAGLAHLTGAPIFISLALLATGLAGL
ALHLHARRSVHRTVVLLDETTARSRAEIETLADRMWEMQESEERFRGLIDALGDLVIHRDRDGYIVYANKVFADLVETDQ
RDLAGKTLAELGIEVGIVPDAAFSDHECLSSTDVAIRTPGGPRWFSWIELSVRDKDSGAASHRAIARDITARKRAESSLI
TARERAEYASQAKSRFLATVSHEIRTPMNGIMGMARLLADTSLSPEQQTYVGAISTSASALLALIEDLLDYSKIEAGRFD
PEPQPMSVREIADNIIELLAARAFAKDIGLGCHVEPDVPQLITADPGRVRQVLLNLIGNAIKFTDGGGVLVSIARARSET
SDRICFTITDTGPGLREEDMERIFEEFEQADGTSTRAHGGAGLGLAISKRLVAAMGGTISVSSRLGQGSEFVFEIPATEA
TEAPQGRQNVLAGRRAVILSRNAVEADAIARTIRANGGTAGIATTVAQAAPLAEGCDVLLVDAAMEESDGRVLKRLRQSG
FTDCEAITLIAPTDRGMLGEFRASGYATFLARPVRGGTLLRVLLTSHAPALAQPQPEKRRTPALRASSDRQQGLSVLIAE
DNDINAMLARATLLKAGHRVKVVGNGKAAVEAVTGAGHKHRFDVVLMDLHMPVMDGLDAIAAIRRHEEETSAPPVPIMVL
SADSQEKTRHAVLAHGASGFVTKPLDPDALVNAVEGQVAA

Sequences:

>Translated_760_residues
MIAESDSQQANKGDDAVAAPAAARRFIAAPPLVPEQQLARREGLPLLTFVAIMVLAGLAHLTGAPIFISLALLATGLAGL
ALHLHARRSVHRTVVLLDETTARSRAEIETLADRMWEMQESEERFRGLIDALGDLVIHRDRDGYIVYANKVFADLVETDQ
RDLAGKTLAELGIEVGIVPDAAFSDHECLSSTDVAIRTPGGPRWFSWIELSVRDKDSGAASHRAIARDITARKRAESSLI
TARERAEYASQAKSRFLATVSHEIRTPMNGIMGMARLLADTSLSPEQQTYVGAISTSASALLALIEDLLDYSKIEAGRFD
PEPQPMSVREIADNIIELLAARAFAKDIGLGCHVEPDVPQLITADPGRVRQVLLNLIGNAIKFTDGGGVLVSIARARSET
SDRICFTITDTGPGLREEDMERIFEEFEQADGTSTRAHGGAGLGLAISKRLVAAMGGTISVSSRLGQGSEFVFEIPATEA
TEAPQGRQNVLAGRRAVILSRNAVEADAIARTIRANGGTAGIATTVAQAAPLAEGCDVLLVDAAMEESDGRVLKRLRQSG
FTDCEAITLIAPTDRGMLGEFRASGYATFLARPVRGGTLLRVLLTSHAPALAQPQPEKRRTPALRASSDRQQGLSVLIAE
DNDINAMLARATLLKAGHRVKVVGNGKAAVEAVTGAGHKHRFDVVLMDLHMPVMDGLDAIAAIRRHEEETSAPPVPIMVL
SADSQEKTRHAVLAHGASGFVTKPLDPDALVNAVEGQVAA
>Mature_760_residues
MIAESDSQQANKGDDAVAAPAAARRFIAAPPLVPEQQLARREGLPLLTFVAIMVLAGLAHLTGAPIFISLALLATGLAGL
ALHLHARRSVHRTVVLLDETTARSRAEIETLADRMWEMQESEERFRGLIDALGDLVIHRDRDGYIVYANKVFADLVETDQ
RDLAGKTLAELGIEVGIVPDAAFSDHECLSSTDVAIRTPGGPRWFSWIELSVRDKDSGAASHRAIARDITARKRAESSLI
TARERAEYASQAKSRFLATVSHEIRTPMNGIMGMARLLADTSLSPEQQTYVGAISTSASALLALIEDLLDYSKIEAGRFD
PEPQPMSVREIADNIIELLAARAFAKDIGLGCHVEPDVPQLITADPGRVRQVLLNLIGNAIKFTDGGGVLVSIARARSET
SDRICFTITDTGPGLREEDMERIFEEFEQADGTSTRAHGGAGLGLAISKRLVAAMGGTISVSSRLGQGSEFVFEIPATEA
TEAPQGRQNVLAGRRAVILSRNAVEADAIARTIRANGGTAGIATTVAQAAPLAEGCDVLLVDAAMEESDGRVLKRLRQSG
FTDCEAITLIAPTDRGMLGEFRASGYATFLARPVRGGTLLRVLLTSHAPALAQPQPEKRRTPALRASSDRQQGLSVLIAE
DNDINAMLARATLLKAGHRVKVVGNGKAAVEAVTGAGHKHRFDVVLMDLHMPVMDGLDAIAAIRRHEEETSAPPVPIMVL
SADSQEKTRHAVLAHGASGFVTKPLDPDALVNAVEGQVAA

Specific function: Forms part of a two-component regulatory system gacA/gacS(lemA). May be involved in lesion formation, swarming and in the production of extracellular protease, syringomycin and N- acyl-L-homoserine lactone (acyl-HSL). Required for pathogenicity on bean [H

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1789149, Length=520, Percent_Identity=32.3076923076923, Blast_Score=251, Evalue=1e-67,
Organism=Escherichia coli, GI145693157, Length=524, Percent_Identity=32.4427480916031, Blast_Score=226, Evalue=4e-60,
Organism=Escherichia coli, GI48994928, Length=395, Percent_Identity=31.8987341772152, Blast_Score=182, Evalue=5e-47,
Organism=Escherichia coli, GI87081816, Length=323, Percent_Identity=36.2229102167183, Blast_Score=167, Evalue=2e-42,
Organism=Escherichia coli, GI1788713, Length=255, Percent_Identity=36.8627450980392, Blast_Score=157, Evalue=3e-39,
Organism=Escherichia coli, GI1790436, Length=230, Percent_Identity=30.8695652173913, Blast_Score=96, Evalue=7e-21,
Organism=Escherichia coli, GI1788393, Length=237, Percent_Identity=29.957805907173, Blast_Score=96, Evalue=1e-20,
Organism=Escherichia coli, GI1786912, Length=254, Percent_Identity=28.740157480315, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1786783, Length=236, Percent_Identity=27.1186440677966, Blast_Score=91, Evalue=3e-19,
Organism=Escherichia coli, GI87082128, Length=243, Percent_Identity=28.3950617283951, Blast_Score=89, Evalue=1e-18,
Organism=Escherichia coli, GI1788549, Length=267, Percent_Identity=26.5917602996255, Blast_Score=89, Evalue=1e-18,
Organism=Escherichia coli, GI1786600, Length=264, Percent_Identity=26.8939393939394, Blast_Score=86, Evalue=7e-18,
Organism=Escherichia coli, GI1787894, Length=235, Percent_Identity=29.3617021276596, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1790346, Length=245, Percent_Identity=28.9795918367347, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1790300, Length=246, Percent_Identity=26.8292682926829, Blast_Score=73, Evalue=5e-14,
Organism=Escherichia coli, GI1789808, Length=232, Percent_Identity=25.4310344827586, Blast_Score=68, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6322044, Length=376, Percent_Identity=25.2659574468085, Blast_Score=112, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6322000, Length=120, Percent_Identity=31.6666666666667, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011006
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR008207
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA; PF01627 Hpt; PF00072 Response_reg [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 81119; Mature: 81119

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: PS50112 PAS ; PS50110 RESPONSE_REGULATORY ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIAESDSQQANKGDDAVAAPAAARRFIAAPPLVPEQQLARREGLPLLTFVAIMVLAGLAH
CCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHH
LTGAPIFISLALLATGLAGLALHLHARRSVHRTVVLLDETTARSRAEIETLADRMWEMQE
HCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHH
SEERFRGLIDALGDLVIHRDRDGYIVYANKVFADLVETDQRDLAGKTLAELGIEVGIVPD
HHHHHHHHHHHHHHHEEEECCCCEEEEEHHHHHHHHHCCHHHHHHHHHHHCCCEEEECCC
AAFSDHECLSSTDVAIRTPGGPRWFSWIELSVRDKDSGAASHRAIARDITARKRAESSLI
CCCCCCHHCCCCCEEEECCCCCCEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHH
TARERAEYASQAKSRFLATVSHEIRTPMNGIMGMARLLADTSLSPEQQTYVGAISTSASA
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCHHHEEEEHHHHHHH
LLALIEDLLDYSKIEAGRFDPEPQPMSVREIADNIIELLAARAFAKDIGLGCHVEPDVPQ
HHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
LITADPGRVRQVLLNLIGNAIKFTDGGGVLVSIARARSETSDRICFTITDTGPGLREEDM
EEECCHHHHHHHHHHHHCCCEEEECCCCEEEEEHHHCCCCCCCEEEEEECCCCCCCHHHH
ERIFEEFEQADGTSTRAHGGAGLGLAISKRLVAAMGGTISVSSRLGQGSEFVFEIPATEA
HHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEHHHCCCCCCEEEECCCCCC
TEAPQGRQNVLAGRRAVILSRNAVEADAIARTIRANGGTAGIATTVAQAAPLAEGCDVLL
CCCCCCHHHHHHCCEEEEEECCCCHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCEEE
VDAAMEESDGRVLKRLRQSGFTDCEAITLIAPTDRGMLGEFRASGYATFLARPVRGGTLL
EECHHCCCCCHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHCCEEHHHCCCCCCCHHH
RVLLTSHAPALAQPQPEKRRTPALRASSDRQQGLSVLIAEDNDINAMLARATLLKAGHRV
HHHHHCCCCCCCCCCCHHHCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCEE
KVVGNGKAAVEAVTGAGHKHRFDVVLMDLHMPVMDGLDAIAAIRRHEEETSAPPVPIMVL
EEEECCCHHHHHHHCCCCCEEEEEEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCEEEEE
SADSQEKTRHAVLAHGASGFVTKPLDPDALVNAVEGQVAA
ECCCHHHHHHHHHEECCCCCEECCCCHHHHHHHHCCCCCC
>Mature Secondary Structure
MIAESDSQQANKGDDAVAAPAAARRFIAAPPLVPEQQLARREGLPLLTFVAIMVLAGLAH
CCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHH
LTGAPIFISLALLATGLAGLALHLHARRSVHRTVVLLDETTARSRAEIETLADRMWEMQE
HCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHH
SEERFRGLIDALGDLVIHRDRDGYIVYANKVFADLVETDQRDLAGKTLAELGIEVGIVPD
HHHHHHHHHHHHHHHEEEECCCCEEEEEHHHHHHHHHCCHHHHHHHHHHHCCCEEEECCC
AAFSDHECLSSTDVAIRTPGGPRWFSWIELSVRDKDSGAASHRAIARDITARKRAESSLI
CCCCCCHHCCCCCEEEECCCCCCEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHH
TARERAEYASQAKSRFLATVSHEIRTPMNGIMGMARLLADTSLSPEQQTYVGAISTSASA
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCHHHEEEEHHHHHHH
LLALIEDLLDYSKIEAGRFDPEPQPMSVREIADNIIELLAARAFAKDIGLGCHVEPDVPQ
HHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
LITADPGRVRQVLLNLIGNAIKFTDGGGVLVSIARARSETSDRICFTITDTGPGLREEDM
EEECCHHHHHHHHHHHHCCCEEEECCCCEEEEEHHHCCCCCCCEEEEEECCCCCCCHHHH
ERIFEEFEQADGTSTRAHGGAGLGLAISKRLVAAMGGTISVSSRLGQGSEFVFEIPATEA
HHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEHHHCCCCCCEEEECCCCCC
TEAPQGRQNVLAGRRAVILSRNAVEADAIARTIRANGGTAGIATTVAQAAPLAEGCDVLL
CCCCCCHHHHHHCCEEEEEECCCCHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCEEE
VDAAMEESDGRVLKRLRQSGFTDCEAITLIAPTDRGMLGEFRASGYATFLARPVRGGTLL
EECHHCCCCCHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHCCEEHHHCCCCCCCHHH
RVLLTSHAPALAQPQPEKRRTPALRASSDRQQGLSVLIAEDNDINAMLARATLLKAGHRV
HHHHHCCCCCCCCCCCHHHCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCEE
KVVGNGKAAVEAVTGAGHKHRFDVVLMDLHMPVMDGLDAIAAIRRHEEETSAPPVPIMVL
EEEECCCHHHHHHHCCCCCEEEEEEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCEEEEE
SADSQEKTRHAVLAHGASGFVTKPLDPDALVNAVEGQVAA
ECCCHHHHHHHHHEECCCCCEECCCCHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1314807 [H]