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The map label for this gene is gltD [H]

Identifier: 13472661

GI number: 13472661

Start: 2433414

End: 2434868

Strand: Reverse

Name: gltD [H]

Synonym: mll3029

Alternate gene names: 13472661

Gene position: 2434868-2433414 (Counterclockwise)

Preceding gene: 13472662

Following gene: 13472658

Centisome position: 34.61

GC content: 65.7

Gene sequence:

>1455_bases
ATGGGCAAGGTAACAGGCTTTCTCGAGATCGACCGGCAGGTGCACAAGTACCAGCCGGCCTCCGACCGTATCCGGCATTT
CCGCGAGTTCACGCTGCCGATGTCGGACAAGGAGGTCGAGAAACAGGCCGCGCGCTGCATGGATTGCGGCATTCCGTTCT
GTCACGGGCCGACGGGCTGTCCGATCCACAACCAGATCCCGGACTGGAACGACCTCGTCTACAACAGGGACTGGGACAAC
GCGATCCGCAACCTGCATTCGACCAACAATTTCCCGGAGTTCACCGGCCGCATCTGTCCCGCCCCTTGCGAGGAAGCCTG
CACGCTGAACCTCGAGGACATTCCGGTCGCCATCAAGACGGTGGAACAGGCGATCGCCGACAAGGCCTACGAGACCGGAC
ACATCAGGCCCTATCCGCCGGAGAAGAAGACCGGCAAGCGGGTCGCCATCATCGGCTCCGGCCCGGCCGGCATGGCGGCC
GCCCAGCAATTGGGCCGCGCCGGCCATGATGTCCATGTCTATGAGCGCGAGAGCCGCCCGGGCGGGCTGATGCGCTACGG
CATTCCCGACTTCAAGATCGAGAAGCACTATATCGACCGGCGCATCGAACAGATGCAGGGCGAGGGCGTGACCTTCCATT
GCGGCGTGAATGTCGGCGTCGACAAGCCCGTCGCCGAACTGCTGGCCGAACATGACGCGGTGCTCTATTGCGGCGGCTCG
GAAACGCCGCGCGCGGCCAACATTCCGGGTGACGATCTCGGCGGCGTGCATGACGCCATGCCCTATCTGGTGCAGCAGAA
CCGCCGCGTCGGCGGCGAGCCGATCCAGTCGGTGGCATGGCCCTCGCATCCGATCATCGCCGGCGGTCAGCATGTCGTCG
TCGTTGGTGGCGGCGATACCGCCTCCGACTGCGTCGGCACCGCCTTCCGCCAGGGCGCGGTGCGCGTCACCCAGCTCGAC
ATCCGCCCGCAGCCGCCCGAGAAGGAAGACAAGCTCGCGGTGTGGCCCTACTGGGCGACCAAGATGCGCACCTCGTCCTC
GCAGGCCGAGGGCGCGGAGCGCGAATTCCAGGTGGCGACGCTCGAATTCATCGGCGAGGACGGCCAACTGACCGGCGTCA
AATGCTGCGAGGTCGATGAGAAGCGCAAGCCGATCGCCGGCACCGAATTCGTCATCCGCGCCGATCTCGCCTTCATCGCC
ATCGGCTTTGCTGGACCGGCGATGGTGGGCGTGGCGAAGGAACTCGAGGGTGAGATGAAGATCACCACCGACAGCCGCCG
CTCCCGGAATGTCGAGGCCAATGACCGCGACTACAAGACCAGTGTCGACCGGCTCTATGCGGCGGGCGACGTGCGCCGTG
GCCAGTCGCTCGTCGTCTGGGCGATCCGCGAAGGCCGCCAGGCGGCGCGCTCGATCGATGAAGCGCTGATGGGGACGAGT
GTGTTGCCGCGCTGA

Upstream 100 bases:

>100_bases
TTCATCGACAGTTTGACCTCGGGCGCAAGGTTGCCATGGCTGCCTCAAGAGGTTAACGGGTGCGGCGAAACCGCACCATC
TGGACAGCAGGAACTGGACT

Downstream 100 bases:

>100_bases
GACCGTTTGGAAACTCTAGTCCCGCGGCCAACACAGGAGCGAGTTTCGAAACAGTCTCAAGAGTTCTGCGTGATTAAGGG
GTGATCGCCGTCGTCGTGTC

Product: glutamate synthase subunit beta

Products: NA

Alternate protein names: NADPH-GOGAT [H]

Number of amino acids: Translated: 484; Mature: 483

Protein sequence:

>484_residues
MGKVTGFLEIDRQVHKYQPASDRIRHFREFTLPMSDKEVEKQAARCMDCGIPFCHGPTGCPIHNQIPDWNDLVYNRDWDN
AIRNLHSTNNFPEFTGRICPAPCEEACTLNLEDIPVAIKTVEQAIADKAYETGHIRPYPPEKKTGKRVAIIGSGPAGMAA
AQQLGRAGHDVHVYERESRPGGLMRYGIPDFKIEKHYIDRRIEQMQGEGVTFHCGVNVGVDKPVAELLAEHDAVLYCGGS
ETPRAANIPGDDLGGVHDAMPYLVQQNRRVGGEPIQSVAWPSHPIIAGGQHVVVVGGGDTASDCVGTAFRQGAVRVTQLD
IRPQPPEKEDKLAVWPYWATKMRTSSSQAEGAEREFQVATLEFIGEDGQLTGVKCCEVDEKRKPIAGTEFVIRADLAFIA
IGFAGPAMVGVAKELEGEMKITTDSRRSRNVEANDRDYKTSVDRLYAAGDVRRGQSLVVWAIREGRQAARSIDEALMGTS
VLPR

Sequences:

>Translated_484_residues
MGKVTGFLEIDRQVHKYQPASDRIRHFREFTLPMSDKEVEKQAARCMDCGIPFCHGPTGCPIHNQIPDWNDLVYNRDWDN
AIRNLHSTNNFPEFTGRICPAPCEEACTLNLEDIPVAIKTVEQAIADKAYETGHIRPYPPEKKTGKRVAIIGSGPAGMAA
AQQLGRAGHDVHVYERESRPGGLMRYGIPDFKIEKHYIDRRIEQMQGEGVTFHCGVNVGVDKPVAELLAEHDAVLYCGGS
ETPRAANIPGDDLGGVHDAMPYLVQQNRRVGGEPIQSVAWPSHPIIAGGQHVVVVGGGDTASDCVGTAFRQGAVRVTQLD
IRPQPPEKEDKLAVWPYWATKMRTSSSQAEGAEREFQVATLEFIGEDGQLTGVKCCEVDEKRKPIAGTEFVIRADLAFIA
IGFAGPAMVGVAKELEGEMKITTDSRRSRNVEANDRDYKTSVDRLYAAGDVRRGQSLVVWAIREGRQAARSIDEALMGTS
VLPR
>Mature_483_residues
GKVTGFLEIDRQVHKYQPASDRIRHFREFTLPMSDKEVEKQAARCMDCGIPFCHGPTGCPIHNQIPDWNDLVYNRDWDNA
IRNLHSTNNFPEFTGRICPAPCEEACTLNLEDIPVAIKTVEQAIADKAYETGHIRPYPPEKKTGKRVAIIGSGPAGMAAA
QQLGRAGHDVHVYERESRPGGLMRYGIPDFKIEKHYIDRRIEQMQGEGVTFHCGVNVGVDKPVAELLAEHDAVLYCGGSE
TPRAANIPGDDLGGVHDAMPYLVQQNRRVGGEPIQSVAWPSHPIIAGGQHVVVVGGGDTASDCVGTAFRQGAVRVTQLDI
RPQPPEKEDKLAVWPYWATKMRTSSSQAEGAEREFQVATLEFIGEDGQLTGVKCCEVDEKRKPIAGTEFVIRADLAFIAI
GFAGPAMVGVAKELEGEMKITTDSRRSRNVEANDRDYKTSVDRLYAAGDVRRGQSLVVWAIREGRQAARSIDEALMGTSV
LPR

Specific function: NITROGEN METABOLISM, GLUTAMATE BIOSYNTHESIS. THE CATALYZED REACTION BRINGS TOGETHER THE NITROGEN AND CARBON METABOLISM. [C]

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=354, Percent_Identity=28.5310734463277, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI111118983, Length=123, Percent_Identity=34.9593495934959, Blast_Score=66, Evalue=8e-11,
Organism=Homo sapiens, GI111118981, Length=123, Percent_Identity=34.9593495934959, Blast_Score=66, Evalue=8e-11,
Organism=Escherichia coli, GI1789606, Length=491, Percent_Identity=35.030549898167, Blast_Score=256, Evalue=2e-69,
Organism=Escherichia coli, GI1788811, Length=471, Percent_Identity=37.1549893842887, Blast_Score=250, Evalue=1e-67,
Organism=Escherichia coli, GI87082180, Length=469, Percent_Identity=32.8358208955224, Blast_Score=209, Evalue=3e-55,
Organism=Escherichia coli, GI1788468, Length=455, Percent_Identity=27.6923076923077, Blast_Score=146, Evalue=3e-36,
Organism=Escherichia coli, GI1789243, Length=433, Percent_Identity=28.6374133949192, Blast_Score=114, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17570289, Length=502, Percent_Identity=41.6334661354582, Blast_Score=388, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI71984108, Length=318, Percent_Identity=27.0440251572327, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17543860, Length=185, Percent_Identity=34.5945945945946, Blast_Score=82, Evalue=7e-16,
Organism=Saccharomyces cerevisiae, GI6320030, Length=497, Percent_Identity=40.2414486921529, Blast_Score=384, Evalue=1e-107,
Organism=Drosophila melanogaster, GI28574881, Length=495, Percent_Identity=44.4444444444444, Blast_Score=420, Evalue=1e-117,
Organism=Drosophila melanogaster, GI24665539, Length=495, Percent_Identity=44.4444444444444, Blast_Score=420, Evalue=1e-117,
Organism=Drosophila melanogaster, GI24665547, Length=495, Percent_Identity=44.4444444444444, Blast_Score=419, Evalue=1e-117,
Organism=Drosophila melanogaster, GI24665543, Length=495, Percent_Identity=44.4444444444444, Blast_Score=419, Evalue=1e-117,
Organism=Drosophila melanogaster, GI24640763, Length=493, Percent_Identity=24.3407707910751, Blast_Score=105, Evalue=7e-23,
Organism=Drosophila melanogaster, GI18858217, Length=493, Percent_Identity=24.3407707910751, Blast_Score=105, Evalue=7e-23,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006005
- InterPro:   IPR009051
- InterPro:   IPR016040
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.4.1.13 [H]

Molecular weight: Translated: 53237; Mature: 53106

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKVTGFLEIDRQVHKYQPASDRIRHFREFTLPMSDKEVEKQAARCMDCGIPFCHGPTGC
CCCCCCHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCCC
PIHNQIPDWNDLVYNRDWDNAIRNLHSTNNFPEFTGRICPAPCEEACTLNLEDIPVAIKT
CCCCCCCCHHHHCCCCCHHHHHHHHHCCCCCCHHCCCCCCCCCCHHHCCCCHHCCHHHHH
VEQAIADKAYETGHIRPYPPEKKTGKRVAIIGSGPAGMAAAQQLGRAGHDVHVYERESRP
HHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHCCCCEEEEEECCCCC
GGLMRYGIPDFKIEKHYIDRRIEQMQGEGVTFHCGVNVGVDKPVAELLAEHDAVLYCGGS
CCEEEECCCCCEEHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHCCCEEEEECCC
ETPRAANIPGDDLGGVHDAMPYLVQQNRRVGGEPIQSVAWPSHPIIAGGQHVVVVGGGDT
CCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHCCCCCCEEECCCEEEEEECCCC
ASDCVGTAFRQGAVRVTQLDIRPQPPEKEDKLAVWPYWATKMRTSSSQAEGAEREFQVAT
HHHHHHHHHHCCCEEEEEEECCCCCCCCCCCEEEEECHHHHHCCCCCCCCCCCHHEEEEE
LEFIGEDGQLTGVKCCEVDEKRKPIAGTEFVIRADLAFIAIGFAGPAMVGVAKELEGEMK
EEEECCCCCCCCEEEECCCCCCCCCCCCEEEEEECEEEEEEECCCHHHHHHHHHCCCCEE
ITTDSRRSRNVEANDRDYKTSVDRLYAAGDVRRGQSLVVWAIREGRQAARSIDEALMGTS
EEECCHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHCCC
VLPR
CCCC
>Mature Secondary Structure 
GKVTGFLEIDRQVHKYQPASDRIRHFREFTLPMSDKEVEKQAARCMDCGIPFCHGPTGC
CCCCCHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCCC
PIHNQIPDWNDLVYNRDWDNAIRNLHSTNNFPEFTGRICPAPCEEACTLNLEDIPVAIKT
CCCCCCCCHHHHCCCCCHHHHHHHHHCCCCCCHHCCCCCCCCCCHHHCCCCHHCCHHHHH
VEQAIADKAYETGHIRPYPPEKKTGKRVAIIGSGPAGMAAAQQLGRAGHDVHVYERESRP
HHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHCCCCEEEEEECCCCC
GGLMRYGIPDFKIEKHYIDRRIEQMQGEGVTFHCGVNVGVDKPVAELLAEHDAVLYCGGS
CCEEEECCCCCEEHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHCCCEEEEECCC
ETPRAANIPGDDLGGVHDAMPYLVQQNRRVGGEPIQSVAWPSHPIIAGGQHVVVVGGGDT
CCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHCCCCCCEEECCCEEEEEECCCC
ASDCVGTAFRQGAVRVTQLDIRPQPPEKEDKLAVWPYWATKMRTSSSQAEGAEREFQVAT
HHHHHHHHHHCCCEEEEEEECCCCCCCCCCCEEEEECHHHHHCCCCCCCCCCCHHEEEEE
LEFIGEDGQLTGVKCCEVDEKRKPIAGTEFVIRADLAFIAIGFAGPAMVGVAKELEGEMK
EEEECCCCCCCCEEEECCCCCCCCCCCCEEEEEECEEEEEEECCCHHHHHHHHHCCCCEE
ITTDSRRSRNVEANDRDYKTSVDRLYAAGDVRRGQSLVVWAIREGRQAARSIDEALMGTS
EEECCHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHCCC
VLPR
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 11029411 [H]