Click here to switch to the map view.

The map label for this gene is eda [H]

Identifier: 13472636

GI number: 13472636

Start: 2403033

End: 2403683

Strand: Direct

Name: eda [H]

Synonym: mlr2994

Alternate gene names: 13472636

Gene position: 2403033-2403683 (Clockwise)

Preceding gene: 13472631

Following gene: 13472638

Centisome position: 34.15

GC content: 67.59

Gene sequence:

>651_bases
ATGGAGAGACAGATGCCCAGCAAGACCGAAAAACTCCTGTCGCTCCTCAACGGCCAACCGGTCATCCCGGTGCTGAAGAT
CAATGATGTCGCCAACGCGGTGCCGTTGGCCCGCGCCTTGGCACGCGGCGGCCTGCCGGCGATCGAGATCACGTTGCGGA
CCGCCGATGCGCTGGAAGCGATCCGGCGGGTGGCGGCCGAGGTCGAGGAAGCCATTGTCGGCGCCGGTACCATTCTGGAC
AGCAGGCAGTTCGACGAGGCCGCCAGTGCCGGCTCGCGCTTCATCGTCAGCCCCGGCATCACGCGCGAGCTTCTGGCAGC
CGCCGCCGACAGCGAGGTTCCACTGCTGCCCGGCGCCATCACGCCCGGCGAGATCATGGCCGCGCGCGAGGCGGGCCTGC
GCTTCCTGAAATTCTTCCCGGCCGAGCAGTCTGGCGGCATCGCCTCGCTGAAGGCCTTCGCCTCGCCGCTGGCCGACGTC
AAATTCTGCCCGACCGGCGGCATCACCGGCAAGAACGCCGCCGACTATCTCAGCCTGCCCAACGTCATCTGCGTCGGCGG
CTCCTGGGTCGCGCCCGACGACATGGTCAAGGCCGGCAAGTGGGACGAAATCGAAGCGCTCGCACGCGCTGCCAGCCAGC
TCAGGAAATAG

Upstream 100 bases:

>100_bases
AGAGCCGGTAAGCTGTCCGCTAGCGCGTGACATCGCGACGCGACCGGTTTCGTGGACAGGCAAACACCCGTCTGCTAATC
GGTTGAAATGACCCTTATCT

Downstream 100 bases:

>100_bases
TCGGTCCCCGCTCACCAAGGCAACGGACCATTCTCGTCGAAATATCCGCCGGTAGGCCCGCCAGCCTTCAGCGCCGCCAG
TTGCACGATGATCTCGGCCG

Product: keto-hydroxyglutarate-aldolase/keto-deoxy- phosphogluconate aldolase

Products: NA

Alternate protein names: KDPG-aldolase; Phospho-2-dehydro-3-deoxygluconate aldolase; Phospho-2-keto-3-deoxygluconate aldolase [H]

Number of amino acids: Translated: 216; Mature: 216

Protein sequence:

>216_residues
MERQMPSKTEKLLSLLNGQPVIPVLKINDVANAVPLARALARGGLPAIEITLRTADALEAIRRVAAEVEEAIVGAGTILD
SRQFDEAASAGSRFIVSPGITRELLAAAADSEVPLLPGAITPGEIMAAREAGLRFLKFFPAEQSGGIASLKAFASPLADV
KFCPTGGITGKNAADYLSLPNVICVGGSWVAPDDMVKAGKWDEIEALARAASQLRK

Sequences:

>Translated_216_residues
MERQMPSKTEKLLSLLNGQPVIPVLKINDVANAVPLARALARGGLPAIEITLRTADALEAIRRVAAEVEEAIVGAGTILD
SRQFDEAASAGSRFIVSPGITRELLAAAADSEVPLLPGAITPGEIMAAREAGLRFLKFFPAEQSGGIASLKAFASPLADV
KFCPTGGITGKNAADYLSLPNVICVGGSWVAPDDMVKAGKWDEIEALARAASQLRK
>Mature_216_residues
MERQMPSKTEKLLSLLNGQPVIPVLKINDVANAVPLARALARGGLPAIEITLRTADALEAIRRVAAEVEEAIVGAGTILD
SRQFDEAASAGSRFIVSPGITRELLAAAADSEVPLLPGAITPGEIMAAREAGLRFLKFFPAEQSGGIASLKAFASPLADV
KFCPTGGITGKNAADYLSLPNVICVGGSWVAPDDMVKAGKWDEIEALARAASQLRK

Specific function: KEY ENZYME IN THE ENTNER-DOUDOROFF PATHWAY. PARTICIPATES IN THE REGULATION OF THE INTRACELLULAR LEVEL OF GLYOXYLATE. [C]

COG id: COG0800

COG function: function code G; 2-keto-3-deoxy-6-phosphogluconate aldolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the KHG/KDPG aldolase family [H]

Homologues:

Organism=Escherichia coli, GI1788156, Length=182, Percent_Identity=50.5494505494505, Blast_Score=192, Evalue=1e-50,
Organism=Escherichia coli, GI48994954, Length=200, Percent_Identity=30, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000887
- InterPro:   IPR013785 [H]

Pfam domain/function: PF01081 Aldolase [H]

EC number: =4.1.2.14 [H]

Molecular weight: Translated: 22571; Mature: 22571

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: PS00159 ALDOLASE_KDPG_KHG_1 ; PS00160 ALDOLASE_KDPG_KHG_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERQMPSKTEKLLSLLNGQPVIPVLKINDVANAVPLARALARGGLPAIEITLRTADALEA
CCCCCCHHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHHHCCCCEEEEEEECHHHHHH
IRRVAAEVEEAIVGAGTILDSRQFDEAASAGSRFIVSPGITRELLAAAADSEVPLLPGAI
HHHHHHHHHHHHHHCHHHHCCHHHHHHHHCCCEEEECCCHHHHHHHHHCCCCCCCCCCCC
TPGEIMAAREAGLRFLKFFPAEQSGGIASLKAFASPLADVKFCPTGGITGKNAADYLSLP
CCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCEECCCCCCCCCCCHHHHCCC
NVICVGGSWVAPDDMVKAGKWDEIEALARAASQLRK
CEEEECCCCCCCHHHHHCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MERQMPSKTEKLLSLLNGQPVIPVLKINDVANAVPLARALARGGLPAIEITLRTADALEA
CCCCCCHHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHHHCCCCEEEEEEECHHHHHH
IRRVAAEVEEAIVGAGTILDSRQFDEAASAGSRFIVSPGITRELLAAAADSEVPLLPGAI
HHHHHHHHHHHHHHCHHHHCCHHHHHHHHCCCEEEECCCHHHHHHHHHCCCCCCCCCCCC
TPGEIMAAREAGLRFLKFFPAEQSGGIASLKAFASPLADVKFCPTGGITGKNAADYLSLP
CCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCEECCCCCCCCCCCHHHHCCC
NVICVGGSWVAPDDMVKAGKWDEIEALARAASQLRK
CEEEECCCCCCCHHHHHCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]