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The map label for this gene is leuA

Identifier: 13472480

GI number: 13472480

Start: 2240400

End: 2242076

Strand: Direct

Name: leuA

Synonym: mlr2792

Alternate gene names: 13472480

Gene position: 2240400-2242076 (Clockwise)

Preceding gene: 13472476

Following gene: 13472481

Centisome position: 31.84

GC content: 62.37

Gene sequence:

>1677_bases
ATGCCCGACGCGGCCCGCAAATATCAGCCCTATCCGACCGTCGGCCTCACCGACCGTACCTGGCCCAACAAGGTCATCGA
CAAGGCGCCGATCTGGTGTTCGGTCGACCTGCGCGACGGCAACCAGGCGCTGATCGACCCGATGGGCCATGAGCGCAAGG
CGCGCATGTTCGGCCTGCTGCTCGACATGGGCTTCAAGGAGATCGAGATCGGCTTCCCCTCGGCCTCGCAGACCGATTTC
GACTTCGCCCGCTGGTGCATCGAGGAAGGCAACGTTCCCGCCGACGTCTCGCTGCAGGTGCTGGTGCAGTGCCGGCCCGA
GCTGATCACGCGCACCTTCGAGGCGCTGAAGGGCGCCACCAACCCGATCGTGCATTTCTACAATTCGACCAGCGAATTGC
AGCGCCGCGTCGTCTTCGAAAAGGATGTCGGCGGCATCAAGCGCATCGCCACTGACGCGGCCAAGATGATCACCGACATG
GCGGCCAAGGCCGGCGGCGGCTATCGCTTCGAATACTCGCCGGAAAGCTTTACCGGCACCGAGCTCGAGGTCGCGCTGGA
GATCTGCAACGCCGTCACCGAGATCGTCAGGCCGAAGCCGGACAACAAGCTGATCATCAACCTGCCGTCGACGGTCGAGA
TGTCGACCCCCAACATCTATGCCGACCGCATCGAATGGATGTGCCGCAATCTGGACAACCGAGAGAACCTGATCATCTCG
CTGCATCCCCACAATGACCGCGGCACCGGCATCGCCACCACCGAGCTCGGCTTGATGGCGGGCGCCGACCGCGTCGAAGG
CACGCTGTTCGGTAATGGCGAGCGCACCGGCAATGTCGACATCGTCACGCTGGCGCTCAACATGTACACGCAAGGCGTCG
ATCCGGGCATCGATTGCTCCGACATCAACCGCATGAAGGACGTCTACGAATACTCGAACCAGCTGAAGATCCCCGAGCGT
CACCCCTATGTCGGCGAACTCGTCTACACCGCCTTTTCAGGCTCCCACCAGGACGCCATCAACAAGGGCATGAAGGCGCT
GAAAAAGGCCAATACCAGCCTGTGGGAAGTGCCTTATCTGCCGATCGACCCGGCCGATGTCGGCCGCAGCTACGAGGCGA
TCATCCGCATCAACTCGCAATCGGGCAAGGGCGGCATCGCCTATGTGCTGCAGGCCGATTACGGCCTCAATCTGCCGCGC
AATCTGCAGATCGAATTCAGCCAGGCGATCCAGGCGATCACCGACGCCGAAGGCAAGGAAGTACCGGCCAAGCGCATCCA
TGAGCGCTTCCTCGAAACCTATGTCGACCAGCCGGGCGCGCGCCTGAAGTTCCTTGACCACCACACCTATCCGGACACTG
CGGTCAAGGGCCGGCGCGTGATCGAGGCAGTGATCCTCGACAAGGGCAAGGAAGTGACCATATCAGGCACCGGGACAGGC
CCGATCGACGGCTTCGTCGATGCGCTGTCGCGCCATGTCGGCGTCGAGATGTCGGTGCTCGATTATTCCGAGCACTCGAT
GCAGCGCGGCTCCAATGCGTCGGCCATTTCCTATGTCGAAATGGAATATCCCGGCGGCAAGCTGTTCGGCGCCGGCATCA
ACACCAACATCGTCGCCGCCTCGCTGGAAGCGGTGACGTCGGCTGCCAACCGCATCGTCGGACGCAAGGCGCGGTAG

Upstream 100 bases:

>100_bases
CGGATCCGAGGGTAGCCGGGAGAACAAGACGATGAACGCACGAGAACAAGTCCGCCCTGATGCCGCGGTCGCCGGCGAGG
ATGTGGAACCAGCCCGCGGC

Downstream 100 bases:

>100_bases
CATTTGCCGATTAGCGCGAGAGACCAGAACGGCTCTCGCGCCAGCCATTTTTTTCAAACCGGAACAAGGCTTTGGCCTTA
ATGGCTGGCACTGGCGGCGG

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase

Number of amino acids: Translated: 558; Mature: 557

Protein sequence:

>558_residues
MPDAARKYQPYPTVGLTDRTWPNKVIDKAPIWCSVDLRDGNQALIDPMGHERKARMFGLLLDMGFKEIEIGFPSASQTDF
DFARWCIEEGNVPADVSLQVLVQCRPELITRTFEALKGATNPIVHFYNSTSELQRRVVFEKDVGGIKRIATDAAKMITDM
AAKAGGGYRFEYSPESFTGTELEVALEICNAVTEIVRPKPDNKLIINLPSTVEMSTPNIYADRIEWMCRNLDNRENLIIS
LHPHNDRGTGIATTELGLMAGADRVEGTLFGNGERTGNVDIVTLALNMYTQGVDPGIDCSDINRMKDVYEYSNQLKIPER
HPYVGELVYTAFSGSHQDAINKGMKALKKANTSLWEVPYLPIDPADVGRSYEAIIRINSQSGKGGIAYVLQADYGLNLPR
NLQIEFSQAIQAITDAEGKEVPAKRIHERFLETYVDQPGARLKFLDHHTYPDTAVKGRRVIEAVILDKGKEVTISGTGTG
PIDGFVDALSRHVGVEMSVLDYSEHSMQRGSNASAISYVEMEYPGGKLFGAGINTNIVAASLEAVTSAANRIVGRKAR

Sequences:

>Translated_558_residues
MPDAARKYQPYPTVGLTDRTWPNKVIDKAPIWCSVDLRDGNQALIDPMGHERKARMFGLLLDMGFKEIEIGFPSASQTDF
DFARWCIEEGNVPADVSLQVLVQCRPELITRTFEALKGATNPIVHFYNSTSELQRRVVFEKDVGGIKRIATDAAKMITDM
AAKAGGGYRFEYSPESFTGTELEVALEICNAVTEIVRPKPDNKLIINLPSTVEMSTPNIYADRIEWMCRNLDNRENLIIS
LHPHNDRGTGIATTELGLMAGADRVEGTLFGNGERTGNVDIVTLALNMYTQGVDPGIDCSDINRMKDVYEYSNQLKIPER
HPYVGELVYTAFSGSHQDAINKGMKALKKANTSLWEVPYLPIDPADVGRSYEAIIRINSQSGKGGIAYVLQADYGLNLPR
NLQIEFSQAIQAITDAEGKEVPAKRIHERFLETYVDQPGARLKFLDHHTYPDTAVKGRRVIEAVILDKGKEVTISGTGTG
PIDGFVDALSRHVGVEMSVLDYSEHSMQRGSNASAISYVEMEYPGGKLFGAGINTNIVAASLEAVTSAANRIVGRKAR
>Mature_557_residues
PDAARKYQPYPTVGLTDRTWPNKVIDKAPIWCSVDLRDGNQALIDPMGHERKARMFGLLLDMGFKEIEIGFPSASQTDFD
FARWCIEEGNVPADVSLQVLVQCRPELITRTFEALKGATNPIVHFYNSTSELQRRVVFEKDVGGIKRIATDAAKMITDMA
AKAGGGYRFEYSPESFTGTELEVALEICNAVTEIVRPKPDNKLIINLPSTVEMSTPNIYADRIEWMCRNLDNRENLIISL
HPHNDRGTGIATTELGLMAGADRVEGTLFGNGERTGNVDIVTLALNMYTQGVDPGIDCSDINRMKDVYEYSNQLKIPERH
PYVGELVYTAFSGSHQDAINKGMKALKKANTSLWEVPYLPIDPADVGRSYEAIIRINSQSGKGGIAYVLQADYGLNLPRN
LQIEFSQAIQAITDAEGKEVPAKRIHERFLETYVDQPGARLKFLDHHTYPDTAVKGRRVIEAVILDKGKEVTISGTGTGP
IDGFVDALSRHVGVEMSVLDYSEHSMQRGSNASAISYVEMEYPGGKLFGAGINTNIVAASLEAVTSAANRIVGRKAR

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate)

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 2 subfamily

Homologues:

Organism=Escherichia coli, GI1786261, Length=536, Percent_Identity=24.0671641791045, Blast_Score=103, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6324225, Length=568, Percent_Identity=45.5985915492958, Blast_Score=510, Evalue=1e-145,
Organism=Saccharomyces cerevisiae, GI6324682, Length=569, Percent_Identity=45.3427065026362, Blast_Score=500, Evalue=1e-142,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEU1_RHILO (Q98HN3)

Other databases:

- EMBL:   BA000012
- RefSeq:   NP_104047.1
- ProteinModelPortal:   Q98HN3
- SMR:   Q98HN3
- GeneID:   1226708
- GenomeReviews:   BA000012_GR
- KEGG:   mlo:mlr2792
- NMPDR:   fig|266835.1.peg.2151
- HOGENOM:   HBG289752
- OMA:   VERCNQI
- ProtClustDB:   PRK03739
- BRENDA:   2.3.3.13
- HAMAP:   MF_00572
- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005668
- InterPro:   IPR000891
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR10277:SF2
- SMART:   SM00917
- TIGRFAMs:   TIGR00970

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer; SSF110921 2-isopropylmalate_synth_dimer

EC number: =2.3.3.13

Molecular weight: Translated: 61554; Mature: 61422

Theoretical pI: Translated: 5.54; Mature: 5.54

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1; PS00816 AIPM_HOMOCIT_SYNTH_2; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDAARKYQPYPTVGLTDRTWPNKVIDKAPIWCSVDLRDGNQALIDPMGHERKARMFGLL
CCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCEEEEEECCCCEEEECCCCCHHHHHHHHHH
LDMGFKEIEIGFPSASQTDFDFARWCIEEGNVPADVSLQVLVQCRPELITRTFEALKGAT
HHCCCEEEEEECCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHCCHHHHHHHHHHHCCCC
NPIVHFYNSTSELQRRVVFEKDVGGIKRIATDAAKMITDMAAKAGGGYRFEYSPESFTGT
CCEEEEECCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCC
ELEVALEICNAVTEIVRPKPDNKLIINLPSTVEMSTPNIYADRIEWMCRNLDNRENLIIS
HHHHHHHHHHHHHHHHCCCCCCEEEEECCCEEEECCCCHHHHHHHHHHHCCCCCCCEEEE
LHPHNDRGTGIATTELGLMAGADRVEGTLFGNGERTGNVDIVTLALNMYTQGVDPGIDCS
EECCCCCCCEEEEEHHHHHCCCCCCCEEEEECCCCCCCEEEEEEEEEHHHCCCCCCCCHH
DINRMKDVYEYSNQLKIPERHPYVGELVYTAFSGSHQDAINKGMKALKKANTSLWEVPYL
HHHHHHHHHHHHCCCCCCCCCCCHHHHHEEHHCCCCHHHHHHHHHHHHHCCCCEEECCCC
PIDPADVGRSYEAIIRINSQSGKGGIAYVLQADYGLNLPRNLQIEFSQAIQAITDAEGKE
CCCHHHHCCCEEEEEEEECCCCCCCEEEEEEECCCCCCCCCEEEEHHHHHHHHHCCCCCC
VPAKRIHERFLETYVDQPGARLKFLDHHTYPDTAVKGRRVIEAVILDKGKEVTISGTGTG
CCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCC
PIDGFVDALSRHVGVEMSVLDYSEHSMQRGSNASAISYVEMEYPGGKLFGAGINTNIVAA
CHHHHHHHHHHHCCCEEEEECCCHHHHHCCCCCCEEEEEEEECCCCEEEECCCCCCHHHH
SLEAVTSAANRIVGRKAR
HHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
PDAARKYQPYPTVGLTDRTWPNKVIDKAPIWCSVDLRDGNQALIDPMGHERKARMFGLL
CCCCCCCCCCCCCCCCCCCCCHHHHCCCCCEEEEEECCCCEEEECCCCCHHHHHHHHHH
LDMGFKEIEIGFPSASQTDFDFARWCIEEGNVPADVSLQVLVQCRPELITRTFEALKGAT
HHCCCEEEEEECCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHCCHHHHHHHHHHHCCCC
NPIVHFYNSTSELQRRVVFEKDVGGIKRIATDAAKMITDMAAKAGGGYRFEYSPESFTGT
CCEEEEECCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCC
ELEVALEICNAVTEIVRPKPDNKLIINLPSTVEMSTPNIYADRIEWMCRNLDNRENLIIS
HHHHHHHHHHHHHHHHCCCCCCEEEEECCCEEEECCCCHHHHHHHHHHHCCCCCCCEEEE
LHPHNDRGTGIATTELGLMAGADRVEGTLFGNGERTGNVDIVTLALNMYTQGVDPGIDCS
EECCCCCCCEEEEEHHHHHCCCCCCCEEEEECCCCCCCEEEEEEEEEHHHCCCCCCCCHH
DINRMKDVYEYSNQLKIPERHPYVGELVYTAFSGSHQDAINKGMKALKKANTSLWEVPYL
HHHHHHHHHHHHCCCCCCCCCCCHHHHHEEHHCCCCHHHHHHHHHHHHHCCCCEEECCCC
PIDPADVGRSYEAIIRINSQSGKGGIAYVLQADYGLNLPRNLQIEFSQAIQAITDAEGKE
CCCHHHHCCCEEEEEEEECCCCCCCEEEEEEECCCCCCCCCEEEEHHHHHHHHHCCCCCC
VPAKRIHERFLETYVDQPGARLKFLDHHTYPDTAVKGRRVIEAVILDKGKEVTISGTGTG
CCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCC
PIDGFVDALSRHVGVEMSVLDYSEHSMQRGSNASAISYVEMEYPGGKLFGAGINTNIVAA
CHHHHHHHHHHHCCCEEEEECCCHHHHHCCCCCCEEEEEEEECCCCEEEECCCCCCHHHH
SLEAVTSAANRIVGRKAR
HHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11214968