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The map label for this gene is prpB [C]

Identifier: 13472467

GI number: 13472467

Start: 2226513

End: 2227283

Strand: Reverse

Name: prpB [C]

Synonym: mll2772

Alternate gene names: 13472467

Gene position: 2227283-2226513 (Counterclockwise)

Preceding gene: 13472470

Following gene: 13472462

Centisome position: 31.66

GC content: 66.8

Gene sequence:

>771_bases
ATGATCGACCAGGCCGAGCGCGCGCGGACGTTCCATTCCCTTCACGTCAAGGGCAACCCGATCGTTCTCTACAATGCCTG
GGATCCGGGCTCGGCCAAGATCGTCGAGAAGGCGGGCGCCAAGGCGATCGCGACCGGAAGCTGGCCGGTCGCGGCCGCCT
TCGGCTATGCCGATGGCGAGAAAATCCCGTTGGAACTGGCGCTCGACAACATCAGGCGCATCGTGCAGGCGGTCGACCTG
CCGGTGACCATGGACCTCGAAGGGGGCTATGGCGTCGAGCCGGAAGTCGTCGCCCAGACGGTGACGCGCGCCTTGCAGGC
CGGCGCTATCGGCTTCAATTTCGAGGACCAGATCGTCGGCGGCACCGGCCTGCACGACATCACCGTCCAGGTGAAGCGGG
TCGAAGCGGCCGCGGCCGCCGTCAAGGCCTCGGGTATCCCGGCCTTCCTCAACGCCCGCACCGACATATTCCTCAAGGCC
AAGCCCGATGCGCATGACAAGGCGCTGCTCGACCAGGCCATCGAGCGGGCGCATGCCTATGAAAAGGCCGGCGCAAGCGG
CTTCTTTGCGCCGGGCCTCGGTGACGAGGGCCTCATTGAGGCGCTGTGCAAGGCAGTTGCGCTCCCGGTCAACATCATCG
CGCTGGCGCATGTGCCGCCGCGCCAGCGATTGGCCGAACTCGGCGTTGCCCGCATCAGCCACGGCCCGGTGCCGTATCGG
CAGATGGCCGAATGGCTGGAGGCGAAAGCGCGGCTGGCGATTTCGGGCTAG

Upstream 100 bases:

>100_bases
TCTGTCCGGTGGCCGCCGCTTCGGGGGCTCATGGCACATCGCCAGGGCCGGCGGTCCCGGCTAGGCTGGACTTCCAACAA
GACCAAGACGGGAAACACCC

Downstream 100 bases:

>100_bases
GTCAATCCTCCAGCGTACCCGACCTGGCGTCGGCGCTTTTCCTGTCGCCGACCAGTTTCAAGAGGTCCTCGCCGGCGCGG
ATGATGCGGCGCGAGCGGCG

Product: hypothetical protein

Products: Pyruvate; Succinate [C]

Alternate protein names: NA

Number of amino acids: Translated: 256; Mature: 256

Protein sequence:

>256_residues
MIDQAERARTFHSLHVKGNPIVLYNAWDPGSAKIVEKAGAKAIATGSWPVAAAFGYADGEKIPLELALDNIRRIVQAVDL
PVTMDLEGGYGVEPEVVAQTVTRALQAGAIGFNFEDQIVGGTGLHDITVQVKRVEAAAAAVKASGIPAFLNARTDIFLKA
KPDAHDKALLDQAIERAHAYEKAGASGFFAPGLGDEGLIEALCKAVALPVNIIALAHVPPRQRLAELGVARISHGPVPYR
QMAEWLEAKARLAISG

Sequences:

>Translated_256_residues
MIDQAERARTFHSLHVKGNPIVLYNAWDPGSAKIVEKAGAKAIATGSWPVAAAFGYADGEKIPLELALDNIRRIVQAVDL
PVTMDLEGGYGVEPEVVAQTVTRALQAGAIGFNFEDQIVGGTGLHDITVQVKRVEAAAAAVKASGIPAFLNARTDIFLKA
KPDAHDKALLDQAIERAHAYEKAGASGFFAPGLGDEGLIEALCKAVALPVNIIALAHVPPRQRLAELGVARISHGPVPYR
QMAEWLEAKARLAISG
>Mature_256_residues
MIDQAERARTFHSLHVKGNPIVLYNAWDPGSAKIVEKAGAKAIATGSWPVAAAFGYADGEKIPLELALDNIRRIVQAVDL
PVTMDLEGGYGVEPEVVAQTVTRALQAGAIGFNFEDQIVGGTGLHDITVQVKRVEAAAAAVKASGIPAFLNARTDIFLKA
KPDAHDKALLDQAIERAHAYEKAGASGFFAPGLGDEGLIEALCKAVALPVNIIALAHVPPRQRLAELGVARISHGPVPYR
QMAEWLEAKARLAISG

Specific function: Catalyzes The Formation Of Pyruvate And Succinate From 2-Methylisocitrate (By Similarity). [C]

COG id: COG2513

COG function: function code G; PEP phosphonomutase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786525, Length=218, Percent_Identity=30.7339449541284, Blast_Score=79, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR015813 [H]

Pfam domain/function: NA

EC number: 4.1.3.30 [C]

Molecular weight: Translated: 27091; Mature: 27091

Theoretical pI: Translated: 6.64; Mature: 6.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDQAERARTFHSLHVKGNPIVLYNAWDPGSAKIVEKAGAKAIATGSWPVAAAFGYADGE
CCCHHHHHHHHHEEEECCCEEEEEECCCCCCHHHHHHCCCCEEECCCCCHHEEECCCCCC
KIPLELALDNIRRIVQAVDLPVTMDLEGGYGVEPEVVAQTVTRALQAGAIGFNFEDQIVG
CCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEC
GTGLHDITVQVKRVEAAAAAVKASGIPAFLNARTDIFLKAKPDAHDKALLDQAIERAHAY
CCCCEEEEEEEHHHHHHHHHHHHCCCCCEECCCCEEEEEECCCCHHHHHHHHHHHHHHHH
EKAGASGFFAPGLGDEGLIEALCKAVALPVNIIALAHVPPRQRLAELGVARISHGPVPYR
HHCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHCCCCCCHH
QMAEWLEAKARLAISG
HHHHHHHHHHHEEECC
>Mature Secondary Structure
MIDQAERARTFHSLHVKGNPIVLYNAWDPGSAKIVEKAGAKAIATGSWPVAAAFGYADGE
CCCHHHHHHHHHEEEECCCEEEEEECCCCCCHHHHHHCCCCEEECCCCCHHEEECCCCCC
KIPLELALDNIRRIVQAVDLPVTMDLEGGYGVEPEVVAQTVTRALQAGAIGFNFEDQIVG
CCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEC
GTGLHDITVQVKRVEAAAAAVKASGIPAFLNARTDIFLKAKPDAHDKALLDQAIERAHAY
CCCCEEEEEEEHHHHHHHHHHHHCCCCCEECCCCEEEEEECCCCHHHHHHHHHHHHHHHH
EKAGASGFFAPGLGDEGLIEALCKAVALPVNIIALAHVPPRQRLAELGVARISHGPVPYR
HHCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHCCCCCCHH
QMAEWLEAKARLAISG
HHHHHHHHHHHEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: methylisocitrate [C]

Specific reaction: methylisocitrate <==> Pyruvate + Succinate [C]

General reaction: Elimination [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]