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The map label for this gene is nodL [H]

Identifier: 13472462

GI number: 13472462

Start: 2221524

End: 2222081

Strand: Reverse

Name: nodL [H]

Synonym: mll2768

Alternate gene names: 13472462

Gene position: 2222081-2221524 (Counterclockwise)

Preceding gene: 13472467

Following gene: 13472460

Centisome position: 31.58

GC content: 68.1

Gene sequence:

>558_bases
ATGACGGGAAGCGAACGCGCGAAGATGGCGGCCGGCGAATGGTACACTTGCCTCGATGACGAACTGGAGGCGCTGCGTGC
CCTCGCGCGCGACGCGGTGTTCGAGCACAATTGCCTGCCGCCGCGGCAGCGCGGCAATCTCGGGCCGGGCCTGAAGGCAT
TGCTCGGCGGCGTGGGCGATGGCGCCCGCGTCGAGGCGCCGTTCCACTGCGCCTACGGCTTAAACATTTTTCTCGGCGAC
GGCGTCTTCCTCAATGCCGGCTGCACCATTCTCGACACGGCAGCCGTACGCATCGGCAAGGGAACGCTGCTTGGCCCCAA
TGTGCAGATCTATTGCGCCGAACACCACAGGCAAGCTGCTGGGCGACAGGCGGGGCTGGAGATCGCCAGGCCGGTCGAGA
TCGGCGCCCATGCCTGGATCGGCGGCAGCGCCATCATCCTTGGCGGCGTCACCATCGGCGAGGGCGCCATCGTCGGCGCC
GGCGCGGTGGTGACGCGTGATGTGCCTGCAAACGAGACGGCGGTCGGCAATCCGGCGCGACCGGTCAAGCGCGGCTGA

Upstream 100 bases:

>100_bases
GACAGGCTACCATAGGTCCGATATTTGGCCAGTACTTGGATTGGCGCTTTTCCGCTGGCCGCGACCATGCTTTGCAACGA
CGTCAATCGGAGACCCCGCC

Downstream 100 bases:

>100_bases
ATTCCCGCCGCAAGCAATCAGGCCTGCGCCACCCGTTCGTCCCGATCGGCCCGCCGGGTGAATTCGCGCCGATACTGCGC
CGGCGATGTCCTCAGCCTCG

Product: acetyltransferase, nodulation protein nodL

Products: CoA; acetylmaltose [C]

Alternate protein names: NA

Number of amino acids: Translated: 185; Mature: 184

Protein sequence:

>185_residues
MTGSERAKMAAGEWYTCLDDELEALRALARDAVFEHNCLPPRQRGNLGPGLKALLGGVGDGARVEAPFHCAYGLNIFLGD
GVFLNAGCTILDTAAVRIGKGTLLGPNVQIYCAEHHRQAAGRQAGLEIARPVEIGAHAWIGGSAIILGGVTIGEGAIVGA
GAVVTRDVPANETAVGNPARPVKRG

Sequences:

>Translated_185_residues
MTGSERAKMAAGEWYTCLDDELEALRALARDAVFEHNCLPPRQRGNLGPGLKALLGGVGDGARVEAPFHCAYGLNIFLGD
GVFLNAGCTILDTAAVRIGKGTLLGPNVQIYCAEHHRQAAGRQAGLEIARPVEIGAHAWIGGSAIILGGVTIGEGAIVGA
GAVVTRDVPANETAVGNPARPVKRG
>Mature_184_residues
TGSERAKMAAGEWYTCLDDELEALRALARDAVFEHNCLPPRQRGNLGPGLKALLGGVGDGARVEAPFHCAYGLNIFLGDG
VFLNAGCTILDTAAVRIGKGTLLGPNVQIYCAEHHRQAAGRQAGLEIARPVEIGAHAWIGGSAIILGGVTIGEGAIVGAG
AVVTRDVPANETAVGNPARPVKRG

Specific function: Acetyltransferase implicated in the O-acetylation of Nod factors [H]

COG id: COG0110

COG function: function code R; Acetyltransferase (isoleucine patch superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transferase hexapeptide repeat family [H]

Homologues:

Organism=Escherichia coli, GI1786664, Length=182, Percent_Identity=42.3076923076923, Blast_Score=144, Evalue=4e-36,
Organism=Escherichia coli, GI1786537, Length=184, Percent_Identity=33.1521739130435, Blast_Score=112, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6322243, Length=178, Percent_Identity=38.7640449438202, Blast_Score=136, Evalue=2e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001451
- InterPro:   IPR018357
- InterPro:   IPR011004 [H]

Pfam domain/function: PF00132 Hexapep [H]

EC number: 2.3.1.79 [C]

Molecular weight: Translated: 19071; Mature: 18940

Theoretical pI: Translated: 7.02; Mature: 7.02

Prosite motif: PS00101 HEXAPEP_TRANSFERASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGSERAKMAAGEWYTCLDDELEALRALARDAVFEHNCLPPRQRGNLGPGLKALLGGVGD
CCCCCCCEECCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCHHHHHCCCCC
GARVEAPFHCAYGLNIFLGDGVFLNAGCTILDTAAVRIGKGTLLGPNVQIYCAEHHRQAA
CCEECCCEEEEECCEEEECCCEEEECCCEEEEHEEEEECCCEEECCCEEEEECCHHHHHC
GRQAGLEIARPVEIGAHAWIGGSAIILGGVTIGEGAIVGAGAVVTRDVPANETAVGNPAR
CHHCCCEEECCEECCCEEEECCCEEEEECEEECCCEEEECCEEEEECCCCCCCCCCCCCC
PVKRG
CCCCC
>Mature Secondary Structure 
TGSERAKMAAGEWYTCLDDELEALRALARDAVFEHNCLPPRQRGNLGPGLKALLGGVGD
CCCCCCEECCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCHHHHHCCCCC
GARVEAPFHCAYGLNIFLGDGVFLNAGCTILDTAAVRIGKGTLLGPNVQIYCAEHHRQAA
CCEECCCEEEEECCEEEECCCEEEECCCEEEEHEEEEECCCEEECCCEEEEECCHHHHHC
GRQAGLEIARPVEIGAHAWIGGSAIILGGVTIGEGAIVGAGAVVTRDVPANETAVGNPAR
CHHCCCEEECCEECCCEEEECCCEEEEECEEECCCEEEECCEEEEECCCCCCCCCCCCCC
PVKRG
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: 0.20 {glucose} 0.11 {maltose}

Km value (mM): 62 {glucose}} 90 {maltose}} 0.018 {acetyl-CoA}} 40 {triethanolamine}} [C]

Substrates: Acetyl-CoA; maltose [C]

Specific reaction: Acetyl-CoA + maltose = CoA + acetylmaltose [C]

General reaction: Acyl group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3132583; 2562395; 2615659 [H]