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The map label for this gene is cca [H]

Identifier: 13472434

GI number: 13472434

Start: 2189628

End: 2190890

Strand: Reverse

Name: cca [H]

Synonym: mll2726

Alternate gene names: 13472434

Gene position: 2190890-2189628 (Counterclockwise)

Preceding gene: 13472435

Following gene: 13472429

Centisome position: 31.14

GC content: 66.35

Gene sequence:

>1263_bases
GTGAGTGTTTCGATCTCGGGCAGGGCCAACTGGCTAGCCGACAAGCATTTGCAGCGCCTGCTTGCCGCCCTGGCTGAAGG
CAGCGAGGAGGCGCGTATCGCTGGTGGCGCCGTGCGCAACACGCTGATGGGCCAGCCGGTCGCCGATGTCGACATCGCCA
CCACCTGTTTGCCGCAAGAAATCATCCGCCGTGCCGAGGCGGAGGGTTTCAAGCCAGTGCCAACCGGCATCGAGCACGGC
ACCGTCACGGTGGTCGCCGGCGGCAAACCCTATGAAATCACCACCTTGCGCGCCGATGTCGAGACCGACGGCCGCCGCGC
CAAAGTGTCGTTCGGGCGCGATTGGAAACTCGATGCGGAGCGGCGCGACTTCACCATCAACGCGCTCTATGCCGAGGCCG
ATGGCAGGGTCGTCGACCTGGTCGGCGGCATCGCCGATATCGAGGCGCGGCGGCTGCGCTTCATCGGTGATGCCGAGGCA
CGCATCCGCGAGGATTATCTGCGCATCCTGCGCTTCTTCCGCTTCTTCGCCTGGTATGGCGAAGGCCGGCCTGACGCCGA
GGGGCTGAAGGCCTGCGCCCGGCTGAAGGAGGGCCTGGCGCAACTTTCGGCGGAGCGCATCTGGGCTGAGCTGAAGAAGC
TGCTTTCGGCGTCTGATCCGTCGCGGGCGCTGCTGTGGATGCGGCAGGCCGGTGTGCTGTCCGCCGCGCTGCCGGAAAGC
GAGAAATGGGGTATCGACGCCATCCACGGATTGACCAAGGCCGAGAAGGATCTGGGCTGGGCGGCGGATCCGATGCTGCG
GCTGGAGGCCATCGTGCCGCCGGATGCCGCGCGCATGAAGACGCTCGCCGAGCGGCTGAGGTTTTCGACCGCGGAATCAG
ACCGTTTGCGCCACTGGGCACTGGCCACCGCCGTCGAGCCGAAGGTGACCGAGGCCGAACTGGCGAAAAGGCTCTATCGC
GGCGACCGGCAAGGGCTTGTCGACCGGCTGCGGCTATCGCTTGCCGGAGCCAGGGTGCGCGCCGTCGAGGACAATAACGC
ATTGCTCGAAGCCGGCGGCTTTTCGCGCCTGCTGGCCTTCGCGCTCAAATGGGAAAAACCGGTGTTTCCGCTGAAAGGCG
CCGATCTGACGGCGCTCGGCGCCACGCCGGGGCCGAAGCTGGGCGAAATCCTCAACAACCTCGAGACGGAATGGATCGAG
GCGGGTTTTGCCCCCGATCGCGGCGCGCTGCTCGAACGCGCCGCGAAGGCGCTTGAAACTTAG

Upstream 100 bases:

>100_bases
ACGATCTCGAATGGTTCTTCTACGACATGCCCTATGGCGACCGGCGCATCTGGGGCGTCACCGCCGGCATCATCCGCACG
CTCTATGAAAGGCTCTATGC

Downstream 100 bases:

>100_bases
ACTGGAGCCCCTGGCCGTGCGACCGTGCCGATCAAAAGGCATAGCCGAGGCGACGATTTGACCGGGCGAAACGTCGCTCT
CCTACCTGATCGCCGTCGGC

Product: poly(A) polymerase

Products: NA

Alternate protein names: tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl- transferase; tRNA nucleotidyltransferase; tRNA-NT [H]

Number of amino acids: Translated: 420; Mature: 419

Protein sequence:

>420_residues
MSVSISGRANWLADKHLQRLLAALAEGSEEARIAGGAVRNTLMGQPVADVDIATTCLPQEIIRRAEAEGFKPVPTGIEHG
TVTVVAGGKPYEITTLRADVETDGRRAKVSFGRDWKLDAERRDFTINALYAEADGRVVDLVGGIADIEARRLRFIGDAEA
RIREDYLRILRFFRFFAWYGEGRPDAEGLKACARLKEGLAQLSAERIWAELKKLLSASDPSRALLWMRQAGVLSAALPES
EKWGIDAIHGLTKAEKDLGWAADPMLRLEAIVPPDAARMKTLAERLRFSTAESDRLRHWALATAVEPKVTEAELAKRLYR
GDRQGLVDRLRLSLAGARVRAVEDNNALLEAGGFSRLLAFALKWEKPVFPLKGADLTALGATPGPKLGEILNNLETEWIE
AGFAPDRGALLERAAKALET

Sequences:

>Translated_420_residues
MSVSISGRANWLADKHLQRLLAALAEGSEEARIAGGAVRNTLMGQPVADVDIATTCLPQEIIRRAEAEGFKPVPTGIEHG
TVTVVAGGKPYEITTLRADVETDGRRAKVSFGRDWKLDAERRDFTINALYAEADGRVVDLVGGIADIEARRLRFIGDAEA
RIREDYLRILRFFRFFAWYGEGRPDAEGLKACARLKEGLAQLSAERIWAELKKLLSASDPSRALLWMRQAGVLSAALPES
EKWGIDAIHGLTKAEKDLGWAADPMLRLEAIVPPDAARMKTLAERLRFSTAESDRLRHWALATAVEPKVTEAELAKRLYR
GDRQGLVDRLRLSLAGARVRAVEDNNALLEAGGFSRLLAFALKWEKPVFPLKGADLTALGATPGPKLGEILNNLETEWIE
AGFAPDRGALLERAAKALET
>Mature_419_residues
SVSISGRANWLADKHLQRLLAALAEGSEEARIAGGAVRNTLMGQPVADVDIATTCLPQEIIRRAEAEGFKPVPTGIEHGT
VTVVAGGKPYEITTLRADVETDGRRAKVSFGRDWKLDAERRDFTINALYAEADGRVVDLVGGIADIEARRLRFIGDAEAR
IREDYLRILRFFRFFAWYGEGRPDAEGLKACARLKEGLAQLSAERIWAELKKLLSASDPSRALLWMRQAGVLSAALPESE
KWGIDAIHGLTKAEKDLGWAADPMLRLEAIVPPDAARMKTLAERLRFSTAESDRLRHWALATAVEPKVTEAELAKRLYRG
DRQGLVDRLRLSLAGARVRAVEDNNALLEAGGFSRLLAFALKWEKPVFPLKGADLTALGATPGPKLGEILNNLETEWIEA
GFAPDRGALLERAAKALET

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 3 subfamily [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=408, Percent_Identity=32.843137254902, Blast_Score=197, Evalue=2e-50,
Organism=Escherichia coli, GI87081691, Length=212, Percent_Identity=33.0188679245283, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1789436, Length=265, Percent_Identity=28.6792452830189, Blast_Score=80, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI71995920, Length=422, Percent_Identity=34.3601895734597, Blast_Score=206, Evalue=1e-53,
Organism=Drosophila melanogaster, GI281360112, Length=400, Percent_Identity=33.75, Blast_Score=194, Evalue=1e-49,
Organism=Drosophila melanogaster, GI21357337, Length=400, Percent_Identity=33.75, Blast_Score=194, Evalue=1e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023068
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 46090; Mature: 45959

Theoretical pI: Translated: 7.58; Mature: 7.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVSISGRANWLADKHLQRLLAALAEGSEEARIAGGAVRNTLMGQPVADVDIATTCLPQE
CEEECCCCCHHHHHHHHHHHHHHHHCCCCHHHHCCHHHHHHHCCCCCCCCHHHHHHHHHH
IIRRAEAEGFKPVPTGIEHGTVTVVAGGKPYEITTLRADVETDGRRAKVSFGRDWKLDAE
HHHHHHCCCCCCCCCCCCCCEEEEEECCCCEEEEEEEECCCCCCCEEEEECCCCCCCCCC
RRDFTINALYAEADGRVVDLVGGIADIEARRLRFIGDAEARIREDYLRILRFFRFFAWYG
CCCEEEEEEEECCCCCEEEECCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHC
EGRPDAEGLKACARLKEGLAQLSAERIWAELKKLLSASDPSRALLWMRQAGVLSAALPES
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCHHHHCCCCC
EKWGIDAIHGLTKAEKDLGWAADPMLRLEAIVPPDAARMKTLAERLRFSTAESDRLRHWA
CCCCHHHHHHHHHHHHHCCCCCCCHHEEEEECCCCHHHHHHHHHHHHCCCCCHHHHHHHH
LATAVEPKVTEAELAKRLYRGDRQGLVDRLRLSLAGARVRAVEDNNALLEAGGFSRLLAF
HHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEEECCCCEEECCCHHHHHHH
ALKWEKPVFPLKGADLTALGATPGPKLGEILNNLETEWIEAGFAPDRGALLERAAKALET
HHHCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
SVSISGRANWLADKHLQRLLAALAEGSEEARIAGGAVRNTLMGQPVADVDIATTCLPQE
EEECCCCCHHHHHHHHHHHHHHHHCCCCHHHHCCHHHHHHHCCCCCCCCHHHHHHHHHH
IIRRAEAEGFKPVPTGIEHGTVTVVAGGKPYEITTLRADVETDGRRAKVSFGRDWKLDAE
HHHHHHCCCCCCCCCCCCCCEEEEEECCCCEEEEEEEECCCCCCCEEEEECCCCCCCCCC
RRDFTINALYAEADGRVVDLVGGIADIEARRLRFIGDAEARIREDYLRILRFFRFFAWYG
CCCEEEEEEEECCCCCEEEECCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHC
EGRPDAEGLKACARLKEGLAQLSAERIWAELKKLLSASDPSRALLWMRQAGVLSAALPES
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCHHHHCCCCC
EKWGIDAIHGLTKAEKDLGWAADPMLRLEAIVPPDAARMKTLAERLRFSTAESDRLRHWA
CCCCHHHHHHHHHHHHHCCCCCCCHHEEEEECCCCHHHHHHHHHHHHCCCCCHHHHHHHH
LATAVEPKVTEAELAKRLYRGDRQGLVDRLRLSLAGARVRAVEDNNALLEAGGFSRLLAF
HHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEEECCCCEEECCCHHHHHHH
ALKWEKPVFPLKGADLTALGATPGPKLGEILNNLETEWIEAGFAPDRGALLERAAKALET
HHHCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA