Click here to switch to the map view.

The map label for this gene is ilvE [H]

Identifier: 13472385

GI number: 13472385

Start: 2146140

End: 2147033

Strand: Reverse

Name: ilvE [H]

Synonym: mll2664

Alternate gene names: 13472385

Gene position: 2147033-2146140 (Counterclockwise)

Preceding gene: 13472391

Following gene: 13472384

Centisome position: 30.51

GC content: 61.63

Gene sequence:

>894_bases
ATGGCATCCGTTCCCTTCGATCAACTGGACGGCTTCATCTGGATGAATGGCGAATTCGTTGCGTGGGGCGACGCCAAGAT
CCATGTTCTGACGCACGGTCTGCACTACGCCAGCGCGGTCTTCGAGGGCGAGCGCGCCTATGGCGGCGAGATCTTCAAGC
TCAACGAGCATACCGAGCGCCTGCATGAATCGGCGCGCCTGCTCGGCTTCAAGATTCCCTATTCGGTTGCCGAGCTCAAC
GACGCCTCGACCACGCTGTTGAAGAAGCAAGGGTTCCAGGACGCCTATGTCAGGCCGATCGCCTGGCGCGGCAGCGAGCA
GATGGGCGTTTCGGCGCAGAACAACCGTATCAACGTCGCCATCGCCATCTGGCAGTGGCCGAGCTATTTCGATCCGGCGC
AGAAGCTGAAGGGCATCCGCCTCGATGTCGCGGAATGGCGCCGGCCCGACCCGCGCACGGCGCCGTCGAAATCGAAGGCC
GCCGGCCTCTACATGATCTGCACCATGTCAAAGCACGCCGCCGAAGCAAAAGGCTACGCCGACGCCATGATGCTCGACTG
GCGCGGCCAGGTCGCCGAAGCAACCGGCGCCAACATCTTCTTCGTCAAGGACGGCAAGATCCATACGCCCAAGCCCGATT
GCTTCCTCGACGGCATCACCCGCCGCACGGTCATCGGGCTCGCCAGGGATCGTGGCCTGGAAGTGATCGAACGCGCCATC
CTGCCGGAAGAACTCGAAGGCTTCGAACAATGCTTCCTGACCGGAACCGCGGCGGAAGTAACGCCGGTTTCGGAGATCGG
CCCCTACCGATTCGAGGTTGGCGAAATCGCCAAGAACCTTATGAATGACTATTCTGCGGCGGTTCAGCCGAAGCACGCGA
TCGCCGCAGAATAA

Upstream 100 bases:

>100_bases
CCTTCGCAACACCGGCCGGCGTCTCCAATCACACGGCGCGGCCAAAAAGAACACGCTACGACACTAAGGATATGCGGGCC
GCGACCCGCGGGAGGTTACC

Downstream 100 bases:

>100_bases
CGATAGTCACAGCTTTTCGCGCAAGCAGGGGCGGTTTTCGGGCCGCCCCTTTTATTTCAGCGTTTCTGCATTTGACTTTC
AGTCAATTCGGCGTCTCATG

Product: branched-chain amino acid aminotransferase

Products: NA

Alternate protein names: BCAT [H]

Number of amino acids: Translated: 297; Mature: 296

Protein sequence:

>297_residues
MASVPFDQLDGFIWMNGEFVAWGDAKIHVLTHGLHYASAVFEGERAYGGEIFKLNEHTERLHESARLLGFKIPYSVAELN
DASTTLLKKQGFQDAYVRPIAWRGSEQMGVSAQNNRINVAIAIWQWPSYFDPAQKLKGIRLDVAEWRRPDPRTAPSKSKA
AGLYMICTMSKHAAEAKGYADAMMLDWRGQVAEATGANIFFVKDGKIHTPKPDCFLDGITRRTVIGLARDRGLEVIERAI
LPEELEGFEQCFLTGTAAEVTPVSEIGPYRFEVGEIAKNLMNDYSAAVQPKHAIAAE

Sequences:

>Translated_297_residues
MASVPFDQLDGFIWMNGEFVAWGDAKIHVLTHGLHYASAVFEGERAYGGEIFKLNEHTERLHESARLLGFKIPYSVAELN
DASTTLLKKQGFQDAYVRPIAWRGSEQMGVSAQNNRINVAIAIWQWPSYFDPAQKLKGIRLDVAEWRRPDPRTAPSKSKA
AGLYMICTMSKHAAEAKGYADAMMLDWRGQVAEATGANIFFVKDGKIHTPKPDCFLDGITRRTVIGLARDRGLEVIERAI
LPEELEGFEQCFLTGTAAEVTPVSEIGPYRFEVGEIAKNLMNDYSAAVQPKHAIAAE
>Mature_296_residues
ASVPFDQLDGFIWMNGEFVAWGDAKIHVLTHGLHYASAVFEGERAYGGEIFKLNEHTERLHESARLLGFKIPYSVAELND
ASTTLLKKQGFQDAYVRPIAWRGSEQMGVSAQNNRINVAIAIWQWPSYFDPAQKLKGIRLDVAEWRRPDPRTAPSKSKAA
GLYMICTMSKHAAEAKGYADAMMLDWRGQVAEATGANIFFVKDGKIHTPKPDCFLDGITRRTVIGLARDRGLEVIERAIL
PEELEGFEQCFLTGTAAEVTPVSEIGPYRFEVGEIAKNLMNDYSAAVQPKHAIAAE

Specific function: Acts on leucine, isoleucine and valine [H]

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Escherichia coli, GI48994963, Length=261, Percent_Identity=38.6973180076628, Blast_Score=191, Evalue=6e-50,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.42 [H]

Molecular weight: Translated: 33034; Mature: 32903

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: PS00770 AA_TRANSFER_CLASS_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASVPFDQLDGFIWMNGEFVAWGDAKIHVLTHGLHYASAVFEGERAYGGEIFKLNEHTER
CCCCCHHHCCCEEEECCEEEEECCEEEEEEECCHHHHHHHHCCCCCCCCEEEEECHHHHH
LHESARLLGFKIPYSVAELNDASTTLLKKQGFQDAYVRPIAWRGSEQMGVSAQNNRINVA
HHHHHHHEEEECCCHHHHCCCHHHHHHHHCCCCCHHCCEEEECCCCCCCCCCCCCEEEEE
IAIWQWPSYFDPAQKLKGIRLDVAEWRRPDPRTAPSKSKAAGLYMICTMSKHAAEAKGYA
EEEEECCCCCCHHHHHCCCEEEHHHCCCCCCCCCCCCCCCCCEEEEEECCHHHHHHCCCC
DAMMLDWRGQVAEATGANIFFVKDGKIHTPKPDCFLDGITRRTVIGLARDRGLEVIERAI
CEEEEECCCCCHHCCCCEEEEEECCEEECCCCCHHHCCCHHHHHHHHHHHCCHHHHHHHC
LPEELEGFEQCFLTGTAAEVTPVSEIGPYRFEVGEIAKNLMNDYSAAVQPKHAIAAE
CHHHHHHHHHHHCCCCCCCCCCHHHCCCCEEEHHHHHHHHHHHHHHHCCCCHHCCCC
>Mature Secondary Structure 
ASVPFDQLDGFIWMNGEFVAWGDAKIHVLTHGLHYASAVFEGERAYGGEIFKLNEHTER
CCCCHHHCCCEEEECCEEEEECCEEEEEEECCHHHHHHHHCCCCCCCCEEEEECHHHHH
LHESARLLGFKIPYSVAELNDASTTLLKKQGFQDAYVRPIAWRGSEQMGVSAQNNRINVA
HHHHHHHEEEECCCHHHHCCCHHHHHHHHCCCCCHHCCEEEECCCCCCCCCCCCCEEEEE
IAIWQWPSYFDPAQKLKGIRLDVAEWRRPDPRTAPSKSKAAGLYMICTMSKHAAEAKGYA
EEEEECCCCCCHHHHHCCCEEEHHHCCCCCCCCCCCCCCCCCEEEEEECCHHHHHHCCCC
DAMMLDWRGQVAEATGANIFFVKDGKIHTPKPDCFLDGITRRTVIGLARDRGLEVIERAI
CEEEEECCCCCHHCCCCEEEEEECCEEECCCCCHHHCCCHHHHHHHHHHHCCHHHHHHHC
LPEELEGFEQCFLTGTAAEVTPVSEIGPYRFEVGEIAKNLMNDYSAAVQPKHAIAAE
CHHHHHHHHHHHCCCCCCCCCCHHHCCCCEEEHHHHHHHHHHHHHHHCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]