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The map label for this gene is stp [H]

Identifier: 13472297

GI number: 13472297

Start: 2059916

End: 2061433

Strand: Reverse

Name: stp [H]

Synonym: mll2542

Alternate gene names: 13472297

Gene position: 2061433-2059916 (Counterclockwise)

Preceding gene: 13472299

Following gene: 13472295

Centisome position: 29.3

GC content: 66.53

Gene sequence:

>1518_bases
ATGCCGAAGAACCGCCTGCCGCTCGTCGCCGCCGTCTATCTCGGCACCTTCGTCGCTTTGCTCGACGTCAGCATCGTCAA
TGTCGCGCTGCCGACCATACAGCAGGCGCTGCACACCGATTTCGGTGGACTGCAATGGGTGGTCGATGCCTACACGCTGT
GCCTGTCGGCCTTCATGCTGTCGTCGGGGCTGATCGGCGACCGCTATGGCCGCAAGCGCTCCTGGCTTGCCGGCGTCGGC
ATCTTCGTTGCCGGTTCGCTGATCTGTGCCGTCGCGCCCAATTTGCCGGCGCTGCTCGCCGGTCGTGTGGTGCAAGGCAT
TGCCGGCTCGCTGCTCATTCCAGGCGCGCTGTCGATCCTGACGCAGGCTTTCCCCGATCCGCGCGAGCGGGCCGGCGTCA
TCGGCGGCTGGGCCGCGTTCGCCGCCGTTTCGCTGGTCGTCGGCCCGGTGCTGGGCGGCATTCTGGTGCAGACGGTCGGC
TGGCAGAGCATCTTCCTGATCAATCTGCCGCTCGGCCTTTTCACCATTGTGCTGGGGGCTGTTTCGATCGAGGAGACGTC
GCATCCCGAACATGCCCATCTCGACCTTGTCGGCCTGGCGCTCTCGATCCTGTGGCTCGGCGCGCTGACCTTCGGCCTGA
TCTCGGCCGGCGAGAGCGGCTGGGGCGATCCCAGGACCATCGTGGCCATCATTGTCGGTGTCGTCGGCCTGGCGGTCTTC
CTTGGCTTCGAGGCGCGCACGCCACGGCCCATGCTGCCGCTCGGCCTGTTCCGCGACGTGCGCTTTGCCGTCGCCAACGT
CGCCTCGTTCGCGCTCGGCTTCACCTCCTATAGCTGCGTGTTCTTCTTCTCGATGTTCCTGCAGCAGGTGCAGGGCTGGT
CGCCGACGCAGACCGGCCTGCGCATGGCGCCGGCCTTCCTTGTCCAGATTGTCGTGTCGCCCTGGATCGGCCGACTGAGC
GCCCGCTATGGGCATTCGCTTTTGATGACGGCGGGCTATGTGCTGATCGGGCTTTCGATGCTCGGCATGTGCTGGGCCGG
GCCGTCGACGCCATACGCGCTGCTCTGCGTCCTGTTCACCATCAACGGTCTCGGCAATTCGCTGGCGATCCCGACCGGCA
GTGCCGCTGCCATGTCCTACGCGCCACGCGAGCGCTCCGGTATGGTGTCGGCCGTCATCAACGCCACCCGCCAGAGCGGG
CTGGCCATCGGCATCGCGCTGCTTGGCGCGCTGATGAGCATGCGGGCGACGAGCCTCTTGACCGGATATCTCCGCGATGC
CGGTGTCGCCAATGCCGAAGCGGTGGCTCGTGCCGCCATATCACGGCATGATTTCGCAACCGACGCGGCAATCGGCGAGG
ATGGCGTGCATCGGCTGTTCGCCACCGCCTATGCCGGCGGCTTCCACGCCGCCATGCTGACGGCGGCGATTGCCGCGTTC
CTCACCGCCACTCTGCTGATCATGGTCCTGGCGCCTGCCGAGGCGCGTTCGGCCAGGCAAGCAAGGCAGCAGGCGTAA

Upstream 100 bases:

>100_bases
CGAGACAAGCTTTTTATAATGATTCCAAAGTGGCTTATCTATATTGACTGACCGTCGGTCAGTGGCATAGATGGCTGCGC
GTTTCATGTTGGGATAGACC

Downstream 100 bases:

>100_bases
GGTGCCCCGCCTCCAACAAAAAACCCGGCGATGACTGCGCCGGGTTTCTGTTGTCGTCAGATCGATCCGGTCAGGTCAGC
GATGCCGTGAAGCGCTGGAT

Product: transporter

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: Spectinomycin tetracycline efflux pump [H]

Number of amino acids: Translated: 505; Mature: 504

Protein sequence:

>505_residues
MPKNRLPLVAAVYLGTFVALLDVSIVNVALPTIQQALHTDFGGLQWVVDAYTLCLSAFMLSSGLIGDRYGRKRSWLAGVG
IFVAGSLICAVAPNLPALLAGRVVQGIAGSLLIPGALSILTQAFPDPRERAGVIGGWAAFAAVSLVVGPVLGGILVQTVG
WQSIFLINLPLGLFTIVLGAVSIEETSHPEHAHLDLVGLALSILWLGALTFGLISAGESGWGDPRTIVAIIVGVVGLAVF
LGFEARTPRPMLPLGLFRDVRFAVANVASFALGFTSYSCVFFFSMFLQQVQGWSPTQTGLRMAPAFLVQIVVSPWIGRLS
ARYGHSLLMTAGYVLIGLSMLGMCWAGPSTPYALLCVLFTINGLGNSLAIPTGSAAAMSYAPRERSGMVSAVINATRQSG
LAIGIALLGALMSMRATSLLTGYLRDAGVANAEAVARAAISRHDFATDAAIGEDGVHRLFATAYAGGFHAAMLTAAIAAF
LTATLLIMVLAPAEARSARQARQQA

Sequences:

>Translated_505_residues
MPKNRLPLVAAVYLGTFVALLDVSIVNVALPTIQQALHTDFGGLQWVVDAYTLCLSAFMLSSGLIGDRYGRKRSWLAGVG
IFVAGSLICAVAPNLPALLAGRVVQGIAGSLLIPGALSILTQAFPDPRERAGVIGGWAAFAAVSLVVGPVLGGILVQTVG
WQSIFLINLPLGLFTIVLGAVSIEETSHPEHAHLDLVGLALSILWLGALTFGLISAGESGWGDPRTIVAIIVGVVGLAVF
LGFEARTPRPMLPLGLFRDVRFAVANVASFALGFTSYSCVFFFSMFLQQVQGWSPTQTGLRMAPAFLVQIVVSPWIGRLS
ARYGHSLLMTAGYVLIGLSMLGMCWAGPSTPYALLCVLFTINGLGNSLAIPTGSAAAMSYAPRERSGMVSAVINATRQSG
LAIGIALLGALMSMRATSLLTGYLRDAGVANAEAVARAAISRHDFATDAAIGEDGVHRLFATAYAGGFHAAMLTAAIAAF
LTATLLIMVLAPAEARSARQARQQA
>Mature_504_residues
PKNRLPLVAAVYLGTFVALLDVSIVNVALPTIQQALHTDFGGLQWVVDAYTLCLSAFMLSSGLIGDRYGRKRSWLAGVGI
FVAGSLICAVAPNLPALLAGRVVQGIAGSLLIPGALSILTQAFPDPRERAGVIGGWAAFAAVSLVVGPVLGGILVQTVGW
QSIFLINLPLGLFTIVLGAVSIEETSHPEHAHLDLVGLALSILWLGALTFGLISAGESGWGDPRTIVAIIVGVVGLAVFL
GFEARTPRPMLPLGLFRDVRFAVANVASFALGFTSYSCVFFFSMFLQQVQGWSPTQTGLRMAPAFLVQIVVSPWIGRLSA
RYGHSLLMTAGYVLIGLSMLGMCWAGPSTPYALLCVLFTINGLGNSLAIPTGSAAAMSYAPRERSGMVSAVINATRQSGL
AIGIALLGALMSMRATSLLTGYLRDAGVANAEAVARAAISRHDFATDAAIGEDGVHRLFATAYAGGFHAAMLTAAIAAFL
TATLLIMVLAPAEARSARQARQQA

Specific function: Contributes to spectinomycin and tetracycline resistance [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1790195, Length=414, Percent_Identity=28.5024154589372, Blast_Score=140, Evalue=3e-34,
Organism=Escherichia coli, GI87081983, Length=437, Percent_Identity=29.0617848970252, Blast_Score=137, Evalue=2e-33,
Organism=Escherichia coli, GI1788710, Length=330, Percent_Identity=30, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI1789042, Length=410, Percent_Identity=25.609756097561, Blast_Score=131, Evalue=1e-31,
Organism=Escherichia coli, GI1788392, Length=419, Percent_Identity=28.1622911694511, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1790146, Length=159, Percent_Identity=30.188679245283, Blast_Score=70, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6324954, Length=397, Percent_Identity=23.6775818639798, Blast_Score=89, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6322958, Length=365, Percent_Identity=25.2054794520548, Blast_Score=77, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6325455, Length=173, Percent_Identity=32.3699421965318, Blast_Score=76, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6319770, Length=469, Percent_Identity=20.8955223880597, Blast_Score=68, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6323735, Length=330, Percent_Identity=24.2424242424242, Blast_Score=66, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6321663, Length=152, Percent_Identity=29.6052631578947, Blast_Score=64, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 52706; Mature: 52574

Theoretical pI: Translated: 9.17; Mature: 9.17

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKNRLPLVAAVYLGTFVALLDVSIVNVALPTIQQALHTDFGGLQWVVDAYTLCLSAFML
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
SSGLIGDRYGRKRSWLAGVGIFVAGSLICAVAPNLPALLAGRVVQGIAGSLLIPGALSIL
HCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHH
TQAFPDPRERAGVIGGWAAFAAVSLVVGPVLGGILVQTVGWQSIFLINLPLGLFTIVLGA
HHHCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHH
VSIEETSHPEHAHLDLVGLALSILWLGALTFGLISAGESGWGDPRTIVAIIVGVVGLAVF
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHH
LGFEARTPRPMLPLGLFRDVRFAVANVASFALGFTSYSCVFFFSMFLQQVQGWSPTQTGL
HCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
RMAPAFLVQIVVSPWIGRLSARYGHSLLMTAGYVLIGLSMLGMCWAGPSTPYALLCVLFT
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
INGLGNSLAIPTGSAAAMSYAPRERSGMVSAVINATRQSGLAIGIALLGALMSMRATSLL
HHCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
TGYLRDAGVANAEAVARAAISRHDFATDAAIGEDGVHRLFATAYAGGFHAAMLTAAIAAF
HHHHHHCCCCCHHHHHHHHHHHHCCHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHHHH
LTATLLIMVLAPAEARSARQARQQA
HHHHHHHHHHCCHHHHHHHHHHHCC
>Mature Secondary Structure 
PKNRLPLVAAVYLGTFVALLDVSIVNVALPTIQQALHTDFGGLQWVVDAYTLCLSAFML
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
SSGLIGDRYGRKRSWLAGVGIFVAGSLICAVAPNLPALLAGRVVQGIAGSLLIPGALSIL
HCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHH
TQAFPDPRERAGVIGGWAAFAAVSLVVGPVLGGILVQTVGWQSIFLINLPLGLFTIVLGA
HHHCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHH
VSIEETSHPEHAHLDLVGLALSILWLGALTFGLISAGESGWGDPRTIVAIIVGVVGLAVF
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHH
LGFEARTPRPMLPLGLFRDVRFAVANVASFALGFTSYSCVFFFSMFLQQVQGWSPTQTGL
HCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
RMAPAFLVQIVVSPWIGRLSARYGHSLLMTAGYVLIGLSMLGMCWAGPSTPYALLCVLFT
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
INGLGNSLAIPTGSAAAMSYAPRERSGMVSAVINATRQSGLAIGIALLGALMSMRATSLL
HHCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
TGYLRDAGVANAEAVARAAISRHDFATDAAIGEDGVHRLFATAYAGGFHAAMLTAAIAAF
HHHHHHCCCCCHHHHHHHHHHHHCCHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHHHH
LTATLLIMVLAPAEARSARQARQQA
HHHHHHHHHHCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]