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The map label for this gene is potD [H]

Identifier: 13471903

GI number: 13471903

Start: 1661006

End: 1662148

Strand: Direct

Name: potD [H]

Synonym: mlr2023

Alternate gene names: 13471903

Gene position: 1661006-1662148 (Clockwise)

Preceding gene: 13471902

Following gene: 13471904

Centisome position: 23.61

GC content: 62.29

Gene sequence:

>1143_bases
ATGAAACTGTCCAACGGCAAGCGGCTTGAACGGCTGCACGATAGATATCAAAACGGCGATCTCAGCCGCCGCACTTTCCT
GACGCTGACCGCCGCCGCGGCCGCCGCGGTCGGCCTCGACATGCCCTGGATGCGGCAGGCGCTCGCCGCGGTCGAGGAGG
TTCGCTTCGACGGCTGGGGCGGCACGGTGCAGGACGCGATCGACAAATACGCCTTCAAGCCCTACACGGCCAAGACCAAG
ATCAAGGTGGTCCAGGGCACGTTCGGCGACGAGGACGAGATCATCACCAAGATCAAGACCGCCAAGCCCGGCGACTACCA
GGTCATCCATTCCTCCGGCGTCAACTACTACATCAAATATGTGAATGGCGGCCTGACCTCGGAGATCAACGAGGCCAACA
TTCCCAACATGGCCAATGTGCTGCAGCCGATGATCGATCCGTTCCGCAAGCTGACGCCGAAACTCTCGGCCGTGCCTTAC
GACTACGGCACGACAGGCATCGCCTACAACACCAAGGTGATCTCGCCCGAGGAGGCCAAGGAAAAGGGCGCCGCCCTTCT
GCTCGACAAGAAATACGCCGGCAAGGTCGGCGGCTATTCCGACATGACCACTCGCGTCTGGTACGCCGCGCTCGCCACCG
GGCAGGATCCCAACAACCTCAAGGACATGGATGCGATCTGGGCCAAGGTGCGCGAGACGCGCGACCTCGCCAAAAAGTTC
TGGAGCTCCGGCGCGGAGCTGATGGACCTGCTTTCCAAGGGCGAGATCGTGGTGACCGACGCCTGGTCGGGCCGCGTCGC
CGCCTTGCAGGACCAGGGCCATCCGATCGGCTATCTCGACCCGGCCGGTTCCTATGCCTGGATGGAGGACATGCTGATCC
TGAAAGGCTCGCCAATGGCCGAGTGCGAGGAGTTGATCAACTTCATGCTCGACCCGGCGACCGCCATCGCGGTCGCCGAA
GGCCAGAGCTATCCGCCGTCGCTCGATCCGACAAAGGTCAAGCTCACCGAGAAGATCGAGAAGCTGCCGGCCTTCGACAA
GACCGGCACGATGAAGTCGCTGACCTTTGCCGACCCGGTCTACTGGGCGACCAACGAGGACGCCTGGACCAAGCAGTGGA
ACAGGATCTCGAAAGGTGCCTGA

Upstream 100 bases:

>100_bases
TGATGATCCTGGTGACCGTCGGCATCCCGCTGCTCGGCGGCCTGATCCTGCAAAGCCTGACCAGATTGAAACGACGCGGT
GAACCAAAGGAGCAAGCACG

Downstream 100 bases:

>100_bases
CAGAAACGACCTTTCAGAAGTGCCCCGGCCGGAAGCGGCCGGGGTGGCAAGGCCCGGCGCGGGCGCACCCGCTCCGAGCG
GCGCCGGGACCGGCCAGCCG

Product: spermidine/putrescine binding protein of ABC transporter

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: SPBP [H]

Number of amino acids: Translated: 380; Mature: 380

Protein sequence:

>380_residues
MKLSNGKRLERLHDRYQNGDLSRRTFLTLTAAAAAAVGLDMPWMRQALAAVEEVRFDGWGGTVQDAIDKYAFKPYTAKTK
IKVVQGTFGDEDEIITKIKTAKPGDYQVIHSSGVNYYIKYVNGGLTSEINEANIPNMANVLQPMIDPFRKLTPKLSAVPY
DYGTTGIAYNTKVISPEEAKEKGAALLLDKKYAGKVGGYSDMTTRVWYAALATGQDPNNLKDMDAIWAKVRETRDLAKKF
WSSGAELMDLLSKGEIVVTDAWSGRVAALQDQGHPIGYLDPAGSYAWMEDMLILKGSPMAECEELINFMLDPATAIAVAE
GQSYPPSLDPTKVKLTEKIEKLPAFDKTGTMKSLTFADPVYWATNEDAWTKQWNRISKGA

Sequences:

>Translated_380_residues
MKLSNGKRLERLHDRYQNGDLSRRTFLTLTAAAAAAVGLDMPWMRQALAAVEEVRFDGWGGTVQDAIDKYAFKPYTAKTK
IKVVQGTFGDEDEIITKIKTAKPGDYQVIHSSGVNYYIKYVNGGLTSEINEANIPNMANVLQPMIDPFRKLTPKLSAVPY
DYGTTGIAYNTKVISPEEAKEKGAALLLDKKYAGKVGGYSDMTTRVWYAALATGQDPNNLKDMDAIWAKVRETRDLAKKF
WSSGAELMDLLSKGEIVVTDAWSGRVAALQDQGHPIGYLDPAGSYAWMEDMLILKGSPMAECEELINFMLDPATAIAVAE
GQSYPPSLDPTKVKLTEKIEKLPAFDKTGTMKSLTFADPVYWATNEDAWTKQWNRISKGA
>Mature_380_residues
MKLSNGKRLERLHDRYQNGDLSRRTFLTLTAAAAAAVGLDMPWMRQALAAVEEVRFDGWGGTVQDAIDKYAFKPYTAKTK
IKVVQGTFGDEDEIITKIKTAKPGDYQVIHSSGVNYYIKYVNGGLTSEINEANIPNMANVLQPMIDPFRKLTPKLSAVPY
DYGTTGIAYNTKVISPEEAKEKGAALLLDKKYAGKVGGYSDMTTRVWYAALATGQDPNNLKDMDAIWAKVRETRDLAKKF
WSSGAELMDLLSKGEIVVTDAWSGRVAALQDQGHPIGYLDPAGSYAWMEDMLILKGSPMAECEELINFMLDPATAIAVAE
GQSYPPSLDPTKVKLTEKIEKLPAFDKTGTMKSLTFADPVYWATNEDAWTKQWNRISKGA

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine. Polyamine binding protein [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787367, Length=278, Percent_Identity=26.978417266187, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 460 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 41991; Mature: 41991

Theoretical pI: Translated: 6.33; Mature: 6.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLSNGKRLERLHDRYQNGDLSRRTFLTLTAAAAAAVGLDMPWMRQALAAVEEVRFDGWG
CCCCCCHHHHHHHHHHCCCCCCHHHEEEHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCC
GTVQDAIDKYAFKPYTAKTKIKVVQGTFGDEDEIITKIKTAKPGDYQVIHSSGVNYYIKY
CHHHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHHHHCCCCCCEEEEECCCCEEEEEE
VNGGLTSEINEANIPNMANVLQPMIDPFRKLTPKLSAVPYDYGTTGIAYNTKVISPEEAK
ECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHCCCCCCCCCCEEEEEEEECCHHHH
EKGAALLLDKKYAGKVGGYSDMTTRVWYAALATGQDPNNLKDMDAIWAKVRETRDLAKKF
HCCCEEEEECHHCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHH
WSSGAELMDLLSKGEIVVTDAWSGRVAALQDQGHPIGYLDPAGSYAWMEDMLILKGSPMA
HHCCHHHHHHHCCCCEEEEECCCCCEEEEECCCCCCCCCCCCCCHHHHHCCCEECCCCHH
ECEELINFMLDPATAIAVAEGQSYPPSLDPTKVKLTEKIEKLPAFDKTGTMKSLTFADPV
HHHHHHHHHHCCCCEEEEECCCCCCCCCCCCEEHHHHHHHHCCCCCCCCCCCEEEECCCE
YWATNEDAWTKQWNRISKGA
EEECCCCHHHHHHHHHCCCC
>Mature Secondary Structure
MKLSNGKRLERLHDRYQNGDLSRRTFLTLTAAAAAAVGLDMPWMRQALAAVEEVRFDGWG
CCCCCCHHHHHHHHHHCCCCCCHHHEEEHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCC
GTVQDAIDKYAFKPYTAKTKIKVVQGTFGDEDEIITKIKTAKPGDYQVIHSSGVNYYIKY
CHHHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHHHHCCCCCCEEEEECCCCEEEEEE
VNGGLTSEINEANIPNMANVLQPMIDPFRKLTPKLSAVPYDYGTTGIAYNTKVISPEEAK
ECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHCCCCCCCCCCEEEEEEEECCHHHH
EKGAALLLDKKYAGKVGGYSDMTTRVWYAALATGQDPNNLKDMDAIWAKVRETRDLAKKF
HCCCEEEEECHHCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHH
WSSGAELMDLLSKGEIVVTDAWSGRVAALQDQGHPIGYLDPAGSYAWMEDMLILKGSPMA
HHCCHHHHHHHCCCCEEEEECCCCCEEEEECCCCCCCCCCCCCCHHHHHCCCEECCCCHH
ECEELINFMLDPATAIAVAEGQSYPPSLDPTKVKLTEKIEKLPAFDKTGTMKSLTFADPV
HHHHHHHHHHCCCCEEEEECCCCCCCCCCCCEEHHHHHHHHCCCCCCCCCCCEEEECCCE
YWATNEDAWTKQWNRISKGA
EEECCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1939142; 8905232; 9278503; 9298646; 8194593; 8621624; 8897598 [H]