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The map label for this gene is gcvA [H]

Identifier: 13471864

GI number: 13471864

Start: 1622981

End: 1623892

Strand: Direct

Name: gcvA [H]

Synonym: mlr1973

Alternate gene names: 13471864

Gene position: 1622981-1623892 (Clockwise)

Preceding gene: 13471861

Following gene: 13471866

Centisome position: 23.07

GC content: 67.54

Gene sequence:

>912_bases
ATGCCGCGAAGATTGCCGCCCCTCACCGCCCTGCCCGCTTTCGACGCCGCCGCAAGGCATCTGAGCTTCTCCAGGGCGGC
GGACGAGCTCAACCTTACCCATGGCGCGGTCAGCCGCGCCATCCGCAACATCGAAGACAGGCTCGGCATCCAGCTTTTCG
ATCGGGGAACGCGATCCGTGCGCCTGACCGCCGTCGGCGCGGCCTATGCGGTCGAGGTCGGCAAGGCGCTCGACCAGATC
GCCGCCGCCACCATCGCCGCCACGCCCGACCGCTCGACGCGCATCCTGAACGTCTCGACGTCGGACGGCTTCGCCGGCCG
CTGGCTGGTGCCGAGGCTGCATCGCTTCCACCGCGCCAATGCCGATATCGACGTGCGACTGGCGACCTCGGGTGTGCTGG
CCGATTTCGTCAGCGACGGCATCGACATCGCCATCCGCTATGGCAAGGGCGGCTACACCGGCGTGACGGCGGAATTTCTC
GCTGACGAGGAGGTCTTCCCGGTCTGCAGCCCGGAACTGCTGCAAGGTGAACATCCGCTGCGCCTGCCCGGGGATCTCAG
GCACCACAAATTGATCCATGACGCCTTTCGCATCGACTGGGCGACATGGCTGCAACAGGCCGGCGTCGATGGCATCGACC
CCAAGCGCGGTGTGCGCTTCGATTCCGCCACCTTCGCGGTCGAGGCCGCCGTGCATGGCGAAGGCGTGCTGCTCGGCCGC
AGCGCGCTGGTCTCCGCCGACCTCGCGGCGGGACGGCTGGTGCGGCCCTTCGACCTCGCGCTGAAATCGGCGGCCAGCTA
CTACCTCGTCTATCCCGACGGCGCGTTGCGGCAGAAGAAGATCAGGGCGTTCCGTGACTGGCTGTTTGCCGAGGTGGCGG
CTGACTGGGTCGGGATGTCTCGGAGGGATTGA

Upstream 100 bases:

>100_bases
ATGTCTGCGCGCCGGTACGCCCTGATATTGGGCATTTCTGGGCTGGACGACAAACCACTCTTTGTGCAGGATTTGTGAGC
AATGCTCACAGGTCGAGATC

Downstream 100 bases:

>100_bases
GGCGAACCACCCGCCTACGCTTTTGACCCACCCACCAACCCCAGCCGCAACAAACTCTCGGCCGTGGCGACGATAGCCTC
CTCGGCCGGGCGCGGCGTCC

Product: glycine cleavage system transcription activator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 303; Mature: 302

Protein sequence:

>303_residues
MPRRLPPLTALPAFDAAARHLSFSRAADELNLTHGAVSRAIRNIEDRLGIQLFDRGTRSVRLTAVGAAYAVEVGKALDQI
AAATIAATPDRSTRILNVSTSDGFAGRWLVPRLHRFHRANADIDVRLATSGVLADFVSDGIDIAIRYGKGGYTGVTAEFL
ADEEVFPVCSPELLQGEHPLRLPGDLRHHKLIHDAFRIDWATWLQQAGVDGIDPKRGVRFDSATFAVEAAVHGEGVLLGR
SALVSADLAAGRLVRPFDLALKSAASYYLVYPDGALRQKKIRAFRDWLFAEVAADWVGMSRRD

Sequences:

>Translated_303_residues
MPRRLPPLTALPAFDAAARHLSFSRAADELNLTHGAVSRAIRNIEDRLGIQLFDRGTRSVRLTAVGAAYAVEVGKALDQI
AAATIAATPDRSTRILNVSTSDGFAGRWLVPRLHRFHRANADIDVRLATSGVLADFVSDGIDIAIRYGKGGYTGVTAEFL
ADEEVFPVCSPELLQGEHPLRLPGDLRHHKLIHDAFRIDWATWLQQAGVDGIDPKRGVRFDSATFAVEAAVHGEGVLLGR
SALVSADLAAGRLVRPFDLALKSAASYYLVYPDGALRQKKIRAFRDWLFAEVAADWVGMSRRD
>Mature_302_residues
PRRLPPLTALPAFDAAARHLSFSRAADELNLTHGAVSRAIRNIEDRLGIQLFDRGTRSVRLTAVGAAYAVEVGKALDQIA
AATIAATPDRSTRILNVSTSDGFAGRWLVPRLHRFHRANADIDVRLATSGVLADFVSDGIDIAIRYGKGGYTGVTAEFLA
DEEVFPVCSPELLQGEHPLRLPGDLRHHKLIHDAFRIDWATWLQQAGVDGIDPKRGVRFDSATFAVEAAVHGEGVLLGRS
ALVSADLAAGRLVRPFDLALKSAASYYLVYPDGALRQKKIRAFRDWLFAEVAADWVGMSRRD

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=295, Percent_Identity=44.0677966101695, Blast_Score=249, Evalue=1e-67,
Organism=Escherichia coli, GI1786448, Length=289, Percent_Identity=34.9480968858132, Blast_Score=156, Evalue=2e-39,
Organism=Escherichia coli, GI1788706, Length=294, Percent_Identity=36.0544217687075, Blast_Score=152, Evalue=3e-38,
Organism=Escherichia coli, GI145693193, Length=294, Percent_Identity=27.5510204081633, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1786401, Length=287, Percent_Identity=26.1324041811847, Blast_Score=85, Evalue=5e-18,
Organism=Escherichia coli, GI1787128, Length=295, Percent_Identity=27.1186440677966, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI1787589, Length=140, Percent_Identity=33.5714285714286, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI157672245, Length=165, Percent_Identity=28.4848484848485, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33058; Mature: 32927

Theoretical pI: Translated: 9.00; Mature: 9.00

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
0.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRRLPPLTALPAFDAAARHLSFSRAADELNLTHGAVSRAIRNIEDRLGIQLFDRGTRSV
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCEEEECCCCCEE
RLTAVGAAYAVEVGKALDQIAAATIAATPDRSTRILNVSTSDGFAGRWLVPRLHRFHRAN
EEEEECHHHHHHHHHHHHHHHHHHEEECCCCCEEEEEEECCCCCCCCHHHHHHHHHHHCC
ADIDVRLATSGVLADFVSDGIDIAIRYGKGGYTGVTAEFLADEEVFPVCSPELLQGEHPL
CCEEEEEECCHHHHHHHCCCEEEEEEECCCCCCCCCHHHHCCCCCCCCCCHHHHCCCCCC
RLPGDLRHHKLIHDAFRIDWATWLQQAGVDGIDPKRGVRFDSATFAVEAAVHGEGVLLGR
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEECCCCEEEEEEECCCEEEEEC
SALVSADLAAGRLVRPFDLALKSAASYYLVYPDGALRQKKIRAFRDWLFAEVAADWVGMS
HHHHHHHHHCCCCCCHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
RRD
CCC
>Mature Secondary Structure 
PRRLPPLTALPAFDAAARHLSFSRAADELNLTHGAVSRAIRNIEDRLGIQLFDRGTRSV
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCEEEECCCCCEE
RLTAVGAAYAVEVGKALDQIAAATIAATPDRSTRILNVSTSDGFAGRWLVPRLHRFHRAN
EEEEECHHHHHHHHHHHHHHHHHHEEECCCCCEEEEEEECCCCCCCCHHHHHHHHHHHCC
ADIDVRLATSGVLADFVSDGIDIAIRYGKGGYTGVTAEFLADEEVFPVCSPELLQGEHPL
CCEEEEEECCHHHHHHHCCCEEEEEEECCCCCCCCCHHHHCCCCCCCCCCHHHHCCCCCC
RLPGDLRHHKLIHDAFRIDWATWLQQAGVDGIDPKRGVRFDSATFAVEAAVHGEGVLLGR
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEECCCCEEEEEEECCCEEEEEC
SALVSADLAAGRLVRPFDLALKSAASYYLVYPDGALRQKKIRAFRDWLFAEVAADWVGMS
HHHHHHHHHCCCCCCHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
RRD
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]