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The map label for this gene is ada [H]

Identifier: 13471775

GI number: 13471775

Start: 1533462

End: 1534577

Strand: Direct

Name: ada [H]

Synonym: mlr1858

Alternate gene names: 13471775

Gene position: 1533462-1534577 (Clockwise)

Preceding gene: 13471772

Following gene: 13471776

Centisome position: 21.79

GC content: 69.53

Gene sequence:

>1116_bases
ATGTTCATCACCCTGGCCAAGAATCTGAAATCCACCCCACTGCCGGTCACCGACAATCCGCGGTGGGCGCGCATTGTCGC
GCGCGACAAGTCGGCGGACGGCGAGTTCTGGTATTCGGTGGCGACGACCGGCGTCTACTGCCGGCCCTCCTGCCCGTCGC
GGCGCGCCAACCCCGCCAATGTCCAGCTGCACGACACGCTGGCTCAGGCGAAGGCGACCGGCTTTCGCGCCTGCCGGCGC
TGTAACCCGGATGGTCCCTCGCTGGAGGCTGGCAATGCCGCGATGGTCGCCGACGCCTGCCGCAGGATCGAACAGAGCGA
GGAAGAGCCATCGCTCGGCGAACTGGCTGATGCCGCCGGCCGCAGCGCCGGCTATTTCCACCGCGTCTTCAAGGCAATCA
CCGGGCTGACGCCGAAGGACTATGCTGCCGCACACCGCGCCGCCCGGGTCCGCCAGGGTCTCGAAGACGGCGCCAGCGTC
ACAGTGGCGATCTACGATGCCGGCTTCAACTCAAGCGGACGCTTCTACGAGAAATCCACCGGCATGCTCGGCATGACGCC
GACGCGCTACCGTGCGGGCGGCGCCAATGAGGATATACGCTTCGCCGTCGGCCAGACCTCGCTCGGCGCCATACTGGTCG
CGTCGAGCCGCAAGGGCGTCGCCTCGATCCTGCTCGGTGACGATCCGGACGCGCTGGTGCGCGACCTGCAGGACCGTTTC
CCCAGGGCGCGGCTGATCGGCGGCGACCGCGACTACGAAGCGCTTGTCGCCCGCGTCGTCGGTTTCGTCGAGTCCCCACA
GCTCGGCCTCGACCTGCCGCTCGACGTGCGCGGCACCGCATTCCAGCAGCGCGTCTGGCAGGCCTTGCAGGACATCCCGG
TCGGCGGCACGGTGTCCTATGCCGAGATCGCCGAGCGCATCGGCTCACCCAGAGCAACCCGCGCCATCGCAGCCGCCTGC
GCCGCCAATGCGCACGCGGTCGCCATTCCCTGCCACCGCGTGATCCGCAAGGACGGCGCGCTGTCCGGCTACGCCTGGGG
CGCCGAGCGCAAGCACGCGCTGATTGAACGCGAGGCCGGCGGATCGGCGCGGGCCCACCGGTCCTCGCGGGCCTGA

Upstream 100 bases:

>100_bases
CAAGTGAAAATTCCCAATCTGGGAAGAACCCGTGCGAGGCATCCTGACAGTTGTCAGGAAACGGGTGGCGAGGCCGGCTT
CCGGCGGCTTCAATGCGGCC

Downstream 100 bases:

>100_bases
CGAGGACCGGCGATGCGCACGATGGCAAGGTCATCCGCCGCCGGTGCGAACAGCCAGATCGCCAACGGTGCTGCCATCGA
CGCGAAGTCACAGGTCTTGG

Product: O6-methylguanine-DNA methyltransferase

Products: NA

Alternate protein names: Regulatory protein of adaptative response; O-6-methylguanine-DNA alkyltransferase [H]

Number of amino acids: Translated: 371; Mature: 371

Protein sequence:

>371_residues
MFITLAKNLKSTPLPVTDNPRWARIVARDKSADGEFWYSVATTGVYCRPSCPSRRANPANVQLHDTLAQAKATGFRACRR
CNPDGPSLEAGNAAMVADACRRIEQSEEEPSLGELADAAGRSAGYFHRVFKAITGLTPKDYAAAHRAARVRQGLEDGASV
TVAIYDAGFNSSGRFYEKSTGMLGMTPTRYRAGGANEDIRFAVGQTSLGAILVASSRKGVASILLGDDPDALVRDLQDRF
PRARLIGGDRDYEALVARVVGFVESPQLGLDLPLDVRGTAFQQRVWQALQDIPVGGTVSYAEIAERIGSPRATRAIAAAC
AANAHAVAIPCHRVIRKDGALSGYAWGAERKHALIEREAGGSARAHRSSRA

Sequences:

>Translated_371_residues
MFITLAKNLKSTPLPVTDNPRWARIVARDKSADGEFWYSVATTGVYCRPSCPSRRANPANVQLHDTLAQAKATGFRACRR
CNPDGPSLEAGNAAMVADACRRIEQSEEEPSLGELADAAGRSAGYFHRVFKAITGLTPKDYAAAHRAARVRQGLEDGASV
TVAIYDAGFNSSGRFYEKSTGMLGMTPTRYRAGGANEDIRFAVGQTSLGAILVASSRKGVASILLGDDPDALVRDLQDRF
PRARLIGGDRDYEALVARVVGFVESPQLGLDLPLDVRGTAFQQRVWQALQDIPVGGTVSYAEIAERIGSPRATRAIAAAC
AANAHAVAIPCHRVIRKDGALSGYAWGAERKHALIEREAGGSARAHRSSRA
>Mature_371_residues
MFITLAKNLKSTPLPVTDNPRWARIVARDKSADGEFWYSVATTGVYCRPSCPSRRANPANVQLHDTLAQAKATGFRACRR
CNPDGPSLEAGNAAMVADACRRIEQSEEEPSLGELADAAGRSAGYFHRVFKAITGLTPKDYAAAHRAARVRQGLEDGASV
TVAIYDAGFNSSGRFYEKSTGMLGMTPTRYRAGGANEDIRFAVGQTSLGAILVASSRKGVASILLGDDPDALVRDLQDRF
PRARLIGGDRDYEALVARVVGFVESPQLGLDLPLDVRGTAFQQRVWQALQDIPVGGTVSYAEIAERIGSPRATRAIAAAC
AANAHAVAIPCHRVIRKDGALSGYAWGAERKHALIEREAGGSARAHRSSRA

Specific function: The methylated ADA protein acts as a positive regulator of its own synthesis, as well as that of other proteins. The transcription-activating function of the ADA protein resides in its N-terminus. It activates the transcription of alkA, alkB and aidB [H]

COG id: COG2169

COG function: function code F; Adenosine deaminase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788542, Length=349, Percent_Identity=46.9914040114613, Blast_Score=328, Evalue=3e-91,
Organism=Escherichia coli, GI1787596, Length=88, Percent_Identity=43.1818181818182, Blast_Score=87, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI115533068, Length=162, Percent_Identity=38.8888888888889, Blast_Score=92, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI115533070, Length=127, Percent_Identity=38.5826771653543, Blast_Score=90, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6320001, Length=90, Percent_Identity=38.8888888888889, Blast_Score=86, Evalue=1e-17,
Organism=Drosophila melanogaster, GI17137554, Length=175, Percent_Identity=34.8571428571429, Blast_Score=81, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004026
- InterPro:   IPR016221
- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR018060
- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF02805 Ada_Zn_binding; PF01035 DNA_binding_1; PF00165 HTH_AraC; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 39721; Mature: 39721

Theoretical pI: Translated: 9.54; Mature: 9.54

Prosite motif: PS01124 HTH_ARAC_FAMILY_2 ; PS00374 MGMT ; PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFITLAKNLKSTPLPVTDNPRWARIVARDKSADGEFWYSVATTGVYCRPSCPSRRANPAN
CEEEEECCCCCCCCCCCCCCCEEEEEEECCCCCCCEEEEEECCCEEECCCCCCCCCCCCC
VQLHDTLAQAKATGFRACRRCNPDGPSLEAGNAAMVADACRRIEQSEEEPSLGELADAAG
EEHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHCC
RSAGYFHRVFKAITGLTPKDYAAAHRAARVRQGLEDGASVTVAIYDAGFNSSGRFYEKST
CCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCEEECCC
GMLGMTPTRYRAGGANEDIRFAVGQTSLGAILVASSRKGVASILLGDDPDALVRDLQDRF
CCCCCCCCCCCCCCCCCCEEEEECCCCCCEEEEECCCCCEEEEEECCCHHHHHHHHHHHC
PRARLIGGDRDYEALVARVVGFVESPQLGLDLPLDVRGTAFQQRVWQALQDIPVGGTVSY
CCEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCH
AEIAERIGSPRATRAIAAACAANAHAVAIPCHRVIRKDGALSGYAWGAERKHALIEREAG
HHHHHHHCCCHHHHHHHHHHHCCCCEEEHHHHHHHHHCCCCCCEECCCCHHHHHHCCCCC
GSARAHRSSRA
CCHHHHCCCCC
>Mature Secondary Structure
MFITLAKNLKSTPLPVTDNPRWARIVARDKSADGEFWYSVATTGVYCRPSCPSRRANPAN
CEEEEECCCCCCCCCCCCCCCEEEEEEECCCCCCCEEEEEECCCEEECCCCCCCCCCCCC
VQLHDTLAQAKATGFRACRRCNPDGPSLEAGNAAMVADACRRIEQSEEEPSLGELADAAG
EEHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHCC
RSAGYFHRVFKAITGLTPKDYAAAHRAARVRQGLEDGASVTVAIYDAGFNSSGRFYEKST
CCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCEEECCC
GMLGMTPTRYRAGGANEDIRFAVGQTSLGAILVASSRKGVASILLGDDPDALVRDLQDRF
CCCCCCCCCCCCCCCCCCEEEEECCCCCCEEEEECCCCCEEEEEECCCHHHHHHHHHHHC
PRARLIGGDRDYEALVARVVGFVESPQLGLDLPLDVRGTAFQQRVWQALQDIPVGGTVSY
CCEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCH
AEIAERIGSPRATRAIAAACAANAHAVAIPCHRVIRKDGALSGYAWGAERKHALIEREAG
HHHHHHHCCCHHHHHHHHHHHCCCCEEEHHHHHHHHHCCCCCCEECCCCHHHHHHCCCCC
GSARAHRSSRA
CCHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2987251; 3887409; 9097040; 9278503; 2982792; 3536913; 3009022; 3529081; 1581309; 8202360; 8500619; 8156986 [H]