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The map label for this gene is gcvA [H]

Identifier: 13471758

GI number: 13471758

Start: 1517165

End: 1518064

Strand: Direct

Name: gcvA [H]

Synonym: mlr1832

Alternate gene names: 13471758

Gene position: 1517165-1518064 (Clockwise)

Preceding gene: 13471756

Following gene: 13471760

Centisome position: 21.56

GC content: 67.11

Gene sequence:

>900_bases
ATGCCCGAAGCCTTCCTCAGCCTGCCGCCGCTGAATGCACTGCGCGCCTTCGAGGCCTCGGCGCGCCATCTCAACTTCCG
CATCGCCGCCGAGGAGTTGAATGTGACCCAAGGCGCGGTGGCCCAGCACATTCGCGGCCTGGAGACCCATCTCGGCACGA
AACTGTTCGAGCGGCTGCCCCGCGGCCTGGCGCTGACCGATCAGGGCCGCGCCTATGTGCCCAACATCCGCCGCGCCTTC
GACCTGATCTCGGAGGCAACCTTGCTGCTGCGTCCCGAGCCGGCACGGCTGACGATCAGCGTGACGCCGAGCTTCGCCAC
CAAATGGCTGATCCCCCGGCTGCAGGATTTCATGGCCGACAATCCGCTGGTCGACTTGCGCGTGCTGGCGAGCGAAAGCC
TGTCGAGCTTCCAGGCCGATGGCGTCGACATAGCGGTGCGGCAAGGTCGCCCGCCCTTCGGCGCCGGCCTGGTCGTCGAC
CTGCTGTTTCCCCAGCAGATCATCGCAATCTGCAGCCCGGCCTTGCTGCCGGCCGGCGCCACCGAAATCCCGGCGGCCGA
AATCCAGCATCACGTGCTGCTGCACGACGCGCACAATCTGTGGCCGGAATTCATGGAAAAGGTGGTCGGCCTGAAGATGA
CGGCAGAGCTGAAACGCATGCGCTTCAACCAGACCAGCCTCGCCATCGACGCGGCCATCGCCGGCCAGGGCATAGCGCTG
GCCAGCCGCTTCCTCGTCGCCGCCGACCTCGCCGCCGGCCGGCTCGTCCAGCCGGTCAGCGGCGAAATGCGTGGCACGCA
GGATTTCCATGTCGTGATGCCGAGAAAGCAGCGCCATCCCGAGCCGACGCAGGCCGTGCGGCAATGGCTGCTGGACGCGG
GGGAACGGGCGTTGTTGTGA

Upstream 100 bases:

>100_bases
ACCGCAAGCGGTGTTTTCGGGATTTGAACGGTGATATAGCTGGTTCTGCCGCGCGGTTCGAACCAGAATGCCTGAAATCA
ACCAGTAGAAAAACTCCTGC

Downstream 100 bases:

>100_bases
GGCCGACACGGCCATTCCCACGACGGTGCGAAGGCCAATCGGATCGACCAATGCATCCTGGCCGATATCACATACTGTCG
GCACATAGCCGATTGCAGTC

Product: transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 299; Mature: 298

Protein sequence:

>299_residues
MPEAFLSLPPLNALRAFEASARHLNFRIAAEELNVTQGAVAQHIRGLETHLGTKLFERLPRGLALTDQGRAYVPNIRRAF
DLISEATLLLRPEPARLTISVTPSFATKWLIPRLQDFMADNPLVDLRVLASESLSSFQADGVDIAVRQGRPPFGAGLVVD
LLFPQQIIAICSPALLPAGATEIPAAEIQHHVLLHDAHNLWPEFMEKVVGLKMTAELKRMRFNQTSLAIDAAIAGQGIAL
ASRFLVAADLAAGRLVQPVSGEMRGTQDFHVVMPRKQRHPEPTQAVRQWLLDAGERALL

Sequences:

>Translated_299_residues
MPEAFLSLPPLNALRAFEASARHLNFRIAAEELNVTQGAVAQHIRGLETHLGTKLFERLPRGLALTDQGRAYVPNIRRAF
DLISEATLLLRPEPARLTISVTPSFATKWLIPRLQDFMADNPLVDLRVLASESLSSFQADGVDIAVRQGRPPFGAGLVVD
LLFPQQIIAICSPALLPAGATEIPAAEIQHHVLLHDAHNLWPEFMEKVVGLKMTAELKRMRFNQTSLAIDAAIAGQGIAL
ASRFLVAADLAAGRLVQPVSGEMRGTQDFHVVMPRKQRHPEPTQAVRQWLLDAGERALL
>Mature_298_residues
PEAFLSLPPLNALRAFEASARHLNFRIAAEELNVTQGAVAQHIRGLETHLGTKLFERLPRGLALTDQGRAYVPNIRRAFD
LISEATLLLRPEPARLTISVTPSFATKWLIPRLQDFMADNPLVDLRVLASESLSSFQADGVDIAVRQGRPPFGAGLVVDL
LFPQQIIAICSPALLPAGATEIPAAEIQHHVLLHDAHNLWPEFMEKVVGLKMTAELKRMRFNQTSLAIDAAIAGQGIALA
SRFLVAADLAAGRLVQPVSGEMRGTQDFHVVMPRKQRHPEPTQAVRQWLLDAGERALL

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=286, Percent_Identity=39.1608391608392, Blast_Score=192, Evalue=2e-50,
Organism=Escherichia coli, GI1786448, Length=302, Percent_Identity=33.112582781457, Blast_Score=136, Evalue=2e-33,
Organism=Escherichia coli, GI1788706, Length=281, Percent_Identity=29.8932384341637, Blast_Score=115, Evalue=3e-27,
Organism=Escherichia coli, GI145693193, Length=288, Percent_Identity=25.3472222222222, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1786401, Length=245, Percent_Identity=24.0816326530612, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32818; Mature: 32686

Theoretical pI: Translated: 7.88; Mature: 7.88

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPEAFLSLPPLNALRAFEASARHLNFRIAAEELNVTQGAVAQHIRGLETHLGTKLFERLP
CCCCCCCCCCHHHHHHHHHHHHHEEEEEEHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCC
RGLALTDQGRAYVPNIRRAFDLISEATLLLRPEPARLTISVTPSFATKWLIPRLQDFMAD
CCCEECCCCCEECCHHHHHHHHHHHCEEEECCCCCEEEEEECCCHHHHHHHHHHHHHHCC
NPLVDLRVLASESLSSFQADGVDIAVRQGRPPFGAGLVVDLLFPQQIIAICSPALLPAGA
CCCEEHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCHHCCCCC
TEIPAAEIQHHVLLHDAHNLWPEFMEKVVGLKMTAELKRMRFNQTSLAIDAAIAGQGIAL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHEEEHHHCCCCHHH
ASRFLVAADLAAGRLVQPVSGEMRGTQDFHVVMPRKQRHPEPTQAVRQWLLDAGERALL
HHHHHHHHHHHHCCEECCCCCCCCCCCCEEEECCCHHCCCCHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
PEAFLSLPPLNALRAFEASARHLNFRIAAEELNVTQGAVAQHIRGLETHLGTKLFERLP
CCCCCCCCCHHHHHHHHHHHHHEEEEEEHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCC
RGLALTDQGRAYVPNIRRAFDLISEATLLLRPEPARLTISVTPSFATKWLIPRLQDFMAD
CCCEECCCCCEECCHHHHHHHHHHHCEEEECCCCCEEEEEECCCHHHHHHHHHHHHHHCC
NPLVDLRVLASESLSSFQADGVDIAVRQGRPPFGAGLVVDLLFPQQIIAICSPALLPAGA
CCCEEHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCHHCCCCC
TEIPAAEIQHHVLLHDAHNLWPEFMEKVVGLKMTAELKRMRFNQTSLAIDAAIAGQGIAL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHEEEHHHCCCCHHH
ASRFLVAADLAAGRLVQPVSGEMRGTQDFHVVMPRKQRHPEPTQAVRQWLLDAGERALL
HHHHHHHHHHHHCCEECCCCCCCCCCCCEEEECCCHHCCCCHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]