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The map label for this gene is afr [H]

Identifier: 13471686

GI number: 13471686

Start: 1450013

End: 1451101

Strand: Reverse

Name: afr [H]

Synonym: mll1735

Alternate gene names: 13471686

Gene position: 1451101-1450013 (Counterclockwise)

Preceding gene: 13471687

Following gene: 13471685

Centisome position: 20.62

GC content: 62.44

Gene sequence:

>1089_bases
ATGGCAGGGACGGATTTCGAACCGGATGTGAAGGTTCGCAGCAGGGAATACCGGATCGGTTGCGTCGGTGCGGGCATGAT
CATGGCCGAATGCCATCTGGCCGCCTATGCGCAAGCAGGCTTCCCGGTGGTGGCGATCGCCTCGCGGACGAAGGCCAGCG
CCGAGAAGGTGGCCAGGCGCTGGGATATTCCGATCGTTCACGATACGCCCGAGCAATTGATCGAGGATTCCAAGGTCGAA
ATCGTCGACCTTGCCTTTCCGCCCGACCAGCAGCCCGCTCTCATCCGTCACGCGCTGAAACAGCCGCACATCAAGGCGAT
CCTGGCGCAGAAGCCGCTGGCGCTGACGGTGGAGGAGGCGATCAAGCTGCGCGACGAGGCCGCCAAGTCAGGCAAGATCC
TCTCGGTCAACCAGAACATGCGCTACGACCAGTCGATGCGCGTCCTGAAGCAGATCATCGACAGCGGTGCGCTGGGCGAC
ATCGTCTTCGCGCAGATCGATATGCATGCGATCCCGCACTGGCAGGCTTTCCTTGCCGATTACGATCGGCTGACGCTTGC
CAATATGAGCGTGCATCATCTCGATGTGCTGCGCTTCCTGTTCGGCGATCCCCACGAAATCACGACGCTGACGCGCAAGG
ATCCGCGCACGACTTTCGACCATTCGGACGGCATCACCGTGTCGACATTGCGCTTCCCCTCCGGGGTGCTCGCCGTTTCG
CTGGAAGATGTCTGGTCCGGTCCGCGCGAGCAGGGCTATCGCGACGACCAGCACATCAACTGGCGTGTCGACGGCACCAA
GGGTGTCGCCAAGGGCACGATCGGCTGGCCGACGGGCGTCGCTTCGACGCTGACCTATGCCTCGACGCAGACGACAGGCG
GCGAATGGGTGACGCCGAGTTGGGACACGATGTGGTTTCCGCATGCCTTCATCGGCGTGATGGAGCAGCTGCAATATGCG
GTGAAGACCGGCACGCCACCGGCCCTCTCCGTTGCCGACAACGTCAAGACCATGGCGCTGGTCGAGGCCGGCTATCGCTC
GATGGCGGAAGGCCGCACCGTCAGGCTTTCTGAAATCTCGACCAACTGA

Upstream 100 bases:

>100_bases
GATTGGTGTCGCCGATGACGGCGGTGATGGGAAATGATGGTGCCGGCCTTGGCTGACCGGCAAGCCGTGGCGCTATATGC
TGTCCTCCAGGGAGGAATGG

Downstream 100 bases:

>100_bases
AAGTACGCCACCGCGCAATAAGCGTGGCGGCTGAATCGTTTACCGGCAGGAGTTCACATCATGATGCAGGCAGGGGTCTT
CACCGGCTACTTTCCCTACG

Product: hypothetical protein

Products: NA

Alternate protein names: Anhydrofructose reductase; 1,5-anhydro-D-fructose reductase (1,5-anhydro-D-mannitol-forming) [H]

Number of amino acids: Translated: 362; Mature: 361

Protein sequence:

>362_residues
MAGTDFEPDVKVRSREYRIGCVGAGMIMAECHLAAYAQAGFPVVAIASRTKASAEKVARRWDIPIVHDTPEQLIEDSKVE
IVDLAFPPDQQPALIRHALKQPHIKAILAQKPLALTVEEAIKLRDEAAKSGKILSVNQNMRYDQSMRVLKQIIDSGALGD
IVFAQIDMHAIPHWQAFLADYDRLTLANMSVHHLDVLRFLFGDPHEITTLTRKDPRTTFDHSDGITVSTLRFPSGVLAVS
LEDVWSGPREQGYRDDQHINWRVDGTKGVAKGTIGWPTGVASTLTYASTQTTGGEWVTPSWDTMWFPHAFIGVMEQLQYA
VKTGTPPALSVADNVKTMALVEAGYRSMAEGRTVRLSEISTN

Sequences:

>Translated_362_residues
MAGTDFEPDVKVRSREYRIGCVGAGMIMAECHLAAYAQAGFPVVAIASRTKASAEKVARRWDIPIVHDTPEQLIEDSKVE
IVDLAFPPDQQPALIRHALKQPHIKAILAQKPLALTVEEAIKLRDEAAKSGKILSVNQNMRYDQSMRVLKQIIDSGALGD
IVFAQIDMHAIPHWQAFLADYDRLTLANMSVHHLDVLRFLFGDPHEITTLTRKDPRTTFDHSDGITVSTLRFPSGVLAVS
LEDVWSGPREQGYRDDQHINWRVDGTKGVAKGTIGWPTGVASTLTYASTQTTGGEWVTPSWDTMWFPHAFIGVMEQLQYA
VKTGTPPALSVADNVKTMALVEAGYRSMAEGRTVRLSEISTN
>Mature_361_residues
AGTDFEPDVKVRSREYRIGCVGAGMIMAECHLAAYAQAGFPVVAIASRTKASAEKVARRWDIPIVHDTPEQLIEDSKVEI
VDLAFPPDQQPALIRHALKQPHIKAILAQKPLALTVEEAIKLRDEAAKSGKILSVNQNMRYDQSMRVLKQIIDSGALGDI
VFAQIDMHAIPHWQAFLADYDRLTLANMSVHHLDVLRFLFGDPHEITTLTRKDPRTTFDHSDGITVSTLRFPSGVLAVSL
EDVWSGPREQGYRDDQHINWRVDGTKGVAKGTIGWPTGVASTLTYASTQTTGGEWVTPSWDTMWFPHAFIGVMEQLQYAV
KTGTPPALSVADNVKTMALVEAGYRSMAEGRTVRLSEISTN

Specific function: Catalyzes the NADPH-specific reduction of 1,5-anhydro-D- fructose to 1,5-anhydro-D-mannitol [H]

COG id: COG0673

COG function: function code R; Predicted dehydrogenases and related proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gfo/idh/mocA family [H]

Homologues:

Organism=Escherichia coli, GI87081947, Length=152, Percent_Identity=27.6315789473684, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR000683
- InterPro:   IPR004104 [H]

Pfam domain/function: PF01408 GFO_IDH_MocA; PF02894 GFO_IDH_MocA_C [H]

EC number: =1.1.1.292 [H]

Molecular weight: Translated: 40001; Mature: 39870

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGTDFEPDVKVRSREYRIGCVGAGMIMAECHLAAYAQAGFPVVAIASRTKASAEKVARR
CCCCCCCCCCEEECCEEEEEEEECCHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHH
WDIPIVHDTPEQLIEDSKVEIVDLAFPPDQQPALIRHALKQPHIKAILAQKPLALTVEEA
CCCCEEECCHHHHHCCCCEEEEEEECCCCCCCHHHHHHHCCCHHHHHHHCCCCEEEHHHH
IKLRDEAAKSGKILSVNQNMRYDQSMRVLKQIIDSGALGDIVFAQIDMHAIPHWQAFLAD
HHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHCCCCCCCEEEEEEHHHCCCHHHHHHHC
YDRLTLANMSVHHLDVLRFLFGDPHEITTLTRKDPRTTFDHSDGITVSTLRFPSGVLAVS
CCCEEEECCCHHHHHHHHHHHCCCHHEEEEECCCCCCCCCCCCCCEEEEEECCCCEEEEE
LEDVWSGPREQGYRDDQHINWRVDGTKGVAKGTIGWPTGVASTLTYASTQTTGGEWVTPS
EHHHHCCHHHCCCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHHEECCCCCCCCCEECCC
WDTMWFPHAFIGVMEQLQYAVKTGTPPALSVADNVKTMALVEAGYRSMAEGRTVRLSEIS
CCCCCHHHHHHHHHHHHHHHHHCCCCCCEEHHCCHHHHHHHHHHHHHHHCCCEEEEEECC
TN
CC
>Mature Secondary Structure 
AGTDFEPDVKVRSREYRIGCVGAGMIMAECHLAAYAQAGFPVVAIASRTKASAEKVARR
CCCCCCCCCEEECCEEEEEEEECCHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHH
WDIPIVHDTPEQLIEDSKVEIVDLAFPPDQQPALIRHALKQPHIKAILAQKPLALTVEEA
CCCCEEECCHHHHHCCCCEEEEEEECCCCCCCHHHHHHHCCCHHHHHHHCCCCEEEHHHH
IKLRDEAAKSGKILSVNQNMRYDQSMRVLKQIIDSGALGDIVFAQIDMHAIPHWQAFLAD
HHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHCCCCCCCEEEEEEHHHCCCHHHHHHHC
YDRLTLANMSVHHLDVLRFLFGDPHEITTLTRKDPRTTFDHSDGITVSTLRFPSGVLAVS
CCCEEEECCCHHHHHHHHHHHCCCHHEEEEECCCCCCCCCCCCCCEEEEEECCCCEEEEE
LEDVWSGPREQGYRDDQHINWRVDGTKGVAKGTIGWPTGVASTLTYASTQTTGGEWVTPS
EHHHHCCHHHCCCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHHEECCCCCCCCCEECCC
WDTMWFPHAFIGVMEQLQYAVKTGTPPALSVADNVKTMALVEAGYRSMAEGRTVRLSEIS
CCCCCHHHHHHHHHHHHHHHHHCCCCCCEEHHCCHHHHHHHHHHHHHHHCCCEEEEEECC
TN
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]