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The map label for this gene is ndhF [H]

Identifier: 13471660

GI number: 13471660

Start: 1421399

End: 1422193

Strand: Direct

Name: ndhF [H]

Synonym: mlr1700

Alternate gene names: 13471660

Gene position: 1421399-1422193 (Clockwise)

Preceding gene: 13471659

Following gene: 13471661

Centisome position: 20.2

GC content: 67.92

Gene sequence:

>795_bases
ATGGCGCTTGCCCTCCAGACTTTCCCGACCGTCAAGGATGCCAACGCGGCACTGAAGGCGGCCGGGACCCGCTATCTCGG
CGGGGGCACACTCGTGGTCCGCGCGGCCAATGAAGGTGATGTTTCGGTCTTCGCCCTTGTCAGATCGACCGACCCGACGC
TCAGGGGGATAGCGGTCGCCGACGGCAAGGCCCGCATCGGCGCATCGGTGACGATGGCGGCGATCGCCCGCCATCCGGAG
CTTGGCGCGCTCGCCAAGGCCGCACGCGCCGTCGGCGGTCCGGCGATCCGCAACATGGCGACCATCGGCGGCAATCTGTT
CGCGCCGGCGCCCTATGGCGACTTCGCCGTGGCCTTGCTGGCGCTCGACGCCACGGTCGGCACCGAGGATGGCGAATTGC
CGATCGAGATCTTCCTGGCCAGGCGGGACGGCAGCCGCTCCATCGTCACGTCAGTCAGTTTCACACTGCCAAAGGCCGAC
GGCTTCCGTTTCCTGAAAGTGTCGCGGGTCAAGCCCAAAGGCGTTTCGGTGCTCAGCATCGCGGCCGTTCTGGAACGGGC
TGCCGACGGCACCGTGTCCTCGGCACGGATCGCGCTTGGCTGCATGGCGGACCGGCCGGTGCGTGCCAAGGCGGCGGAAA
AGGCGCTCGAGGGGAAGAAACTCGACCAGAATGGCATCGCGGCAGCACTGGCCGCTGCCACCGAGGGCATCGCGCCGATC
ACCGATCCGATCGCTAGCGCCTGGTACCGCGCTGAAGTCCTGCCGGTCCATCTCGGCCGGCTGCTGCTGACCTGA

Upstream 100 bases:

>100_bases
CGACAAGATCGCCGCCGGCATGGGCGCCAATGTCTTCCAGGGCGGCTTCAACGCCCTGAAGAGCCATTTTTCTGGCCAGG
ACTGAGAACAGGAGCGTTCC

Downstream 100 bases:

>100_bases
AATCACCGTTGGGGGGCGCGCGTCTGTCCGATCCGTCGGGCAGGCGCGCAAGGAGGAAACATGGCGAAGGTCCCGGTTCA
ATTCATACTCAACGGCTCCG

Product: oxidoreductase

Products: NA

Alternate protein names: NDH; Nicotinic acid hydroxylase FAD-subunit; NAH [H]

Number of amino acids: Translated: 264; Mature: 263

Protein sequence:

>264_residues
MALALQTFPTVKDANAALKAAGTRYLGGGTLVVRAANEGDVSVFALVRSTDPTLRGIAVADGKARIGASVTMAAIARHPE
LGALAKAARAVGGPAIRNMATIGGNLFAPAPYGDFAVALLALDATVGTEDGELPIEIFLARRDGSRSIVTSVSFTLPKAD
GFRFLKVSRVKPKGVSVLSIAAVLERAADGTVSSARIALGCMADRPVRAKAAEKALEGKKLDQNGIAAALAAATEGIAPI
TDPIASAWYRAEVLPVHLGRLLLT

Sequences:

>Translated_264_residues
MALALQTFPTVKDANAALKAAGTRYLGGGTLVVRAANEGDVSVFALVRSTDPTLRGIAVADGKARIGASVTMAAIARHPE
LGALAKAARAVGGPAIRNMATIGGNLFAPAPYGDFAVALLALDATVGTEDGELPIEIFLARRDGSRSIVTSVSFTLPKAD
GFRFLKVSRVKPKGVSVLSIAAVLERAADGTVSSARIALGCMADRPVRAKAAEKALEGKKLDQNGIAAALAAATEGIAPI
TDPIASAWYRAEVLPVHLGRLLLT
>Mature_263_residues
ALALQTFPTVKDANAALKAAGTRYLGGGTLVVRAANEGDVSVFALVRSTDPTLRGIAVADGKARIGASVTMAAIARHPEL
GALAKAARAVGGPAIRNMATIGGNLFAPAPYGDFAVALLALDATVGTEDGELPIEIFLARRDGSRSIVTSVSFTLPKADG
FRFLKVSRVKPKGVSVLSIAAVLERAADGTVSSARIALGCMADRPVRAKAAEKALEGKKLDQNGIAAALAAATEGIAPIT
DPIASAWYRAEVLPVHLGRLLLT

Specific function: Catalyzes the hydroxylation of nicotinate to 6- hydroxynicotinate. Also active against 2-pyrazinecarboxylic acid, but inactive against other nicotinate analogs [H]

COG id: COG1319

COG function: function code C; Aerobic-type carbon monoxide dehydrogenase, middle subunit CoxM/CutM homologs

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding PCMH-type domain [H]

Homologues:

Organism=Escherichia coli, GI1789231, Length=252, Percent_Identity=27.7777777777778, Blast_Score=68, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005107
- InterPro:   IPR016169
- InterPro:   IPR016166
- InterPro:   IPR016167
- InterPro:   IPR002346 [H]

Pfam domain/function: PF03450 CO_deh_flav_C; PF00941 FAD_binding_5 [H]

EC number: =1.17.1.5 [H]

Molecular weight: Translated: 27033; Mature: 26901

Theoretical pI: Translated: 10.46; Mature: 10.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALALQTFPTVKDANAALKAAGTRYLGGGTLVVRAANEGDVSVFALVRSTDPTLRGIAVA
CEEEECCCCCCCCCCHHHHHCCCEEECCCEEEEEECCCCCEEEEEEEECCCCCCEEEEEE
DGKARIGASVTMAAIARHPELGALAKAARAVGGPAIRNMATIGGNLFAPAPYGDFAVALL
CCCCCCCCHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHCCCEECCCCCCHHHHHHH
ALDATVGTEDGELPIEIFLARRDGSRSIVTSVSFTLPKADGFRFLKVSRVKPKGVSVLSI
HHHHHCCCCCCCCEEEEEEEECCCCCEEEEEEEEECCCCCCEEEEEEECCCCCCCHHHHH
AAVLERAADGTVSSARIALGCMADRPVRAKAAEKALEGKKLDQNGIAAALAAATEGIAPI
HHHHHHHCCCCCCCCEEEEEECCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCC
TDPIASAWYRAEVLPVHLGRLLLT
HHHHHHHHHHHEEHHHHHHHHHCC
>Mature Secondary Structure 
ALALQTFPTVKDANAALKAAGTRYLGGGTLVVRAANEGDVSVFALVRSTDPTLRGIAVA
EEEECCCCCCCCCCHHHHHCCCEEECCCEEEEEECCCCCEEEEEEEECCCCCCEEEEEE
DGKARIGASVTMAAIARHPELGALAKAARAVGGPAIRNMATIGGNLFAPAPYGDFAVALL
CCCCCCCCHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHCCCEECCCCCCHHHHHHH
ALDATVGTEDGELPIEIFLARRDGSRSIVTSVSFTLPKADGFRFLKVSRVKPKGVSVLSI
HHHHHCCCCCCCCEEEEEEEECCCCCEEEEEEEEECCCCCCEEEEEEECCCCCCCHHHHH
AAVLERAADGTVSSARIALGCMADRPVRAKAAEKALEGKKLDQNGIAAALAAATEGIAPI
HHHHHHHCCCCCCCCEEEEEECCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCC
TDPIASAWYRAEVLPVHLGRLLLT
HHHHHHHHHHHEEHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA