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The map label for this gene is thyA

Identifier: 13471483

GI number: 13471483

Start: 1220831

End: 1221625

Strand: Reverse

Name: thyA

Synonym: mll1467

Alternate gene names: 13471483

Gene position: 1221625-1220831 (Counterclockwise)

Preceding gene: 13471484

Following gene: 13471481

Centisome position: 17.36

GC content: 62.64

Gene sequence:

>795_bases
ATGCGCCAATATCTCGACCTCTTGCAGCATGTGCTGGACAACGGTGCCGACCGTAGCGACCGCACCGGCACCGGCACGCG
CTCCGTCTTTGGCTACCAGATGCGTTTCGACCTGGCGCGCGGTTTTCCGGTCACTACGACCAAGAAGCTGCACCTGAAGT
CGATCATCCATGAACTCCTGTGGTTCCTGGCCGGCGACACCAACATCAAATACCTGACTGACCATGGCGTCACGATCTGG
GACGAATGGGCCGACGAGAATGGCGACCTTGGTCCCGTCTACGGCAGGCAGTGGCGCTCCTGGCCGGACGGTCATGGCGG
CTCGATCGACCAGATCGCGGGCCTTCTCAAGGAGATACGCCGCAATCCTCAATCGCGGCGGCTGATCGTTTCGGCGTGGA
ACCCGGCCGAGGTGGAGGCGATGGCGCTGCCGCCCTGCCACTGCCTGTTCCAGTTCTACGTGTCCGAGGGCCGGCTGTCC
TGCCAGCTCTACCAGCGGTCGGCCGATATTTTCCTCGGCGTGCCCTTCAACATCGCGTCCTACGCGCTGCTGACGCTGAT
GGTGGCGCAGGTGACCGGGCTGAAGCCGGGCGATTTCGTCCACACGCTGGGCGACGCGCATCTCTATTCGAACCATTTCG
AACAGGCGCGCGAGCAGTTGCAGCGCACGCCAAGGGCATTGCCGACGATGTGGATCAATCCGGAGGTGAAGGACCTGTTC
GCCTTCCGCTTCGAGGATTTCCGGCTGGAAAACTACGTCGCCGACGCATCGATCCAGGCGCCGATCGCCGTTTGA

Upstream 100 bases:

>100_bases
GCTCCGCTTCCTGTCCGGGTGGGGAAAGAATCTCAGACAGGCGTGGCGCCGGCGCACACATGCGCTATGATTGGTGAAAT
CTCGAATCGAGCCGGAACCG

Downstream 100 bases:

>100_bases
GCCGCAGACGGCTCAGTCGATGCCGACCATCACGTCCGAGGCCTTGACCACGGCATAGGCCTGCTTGCCCTTTTCGAACT
TGAGGTCGGCGACGGCTTCA

Product: thymidylate synthase

Products: NA

Alternate protein names: TS; TSase

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MRQYLDLLQHVLDNGADRSDRTGTGTRSVFGYQMRFDLARGFPVTTTKKLHLKSIIHELLWFLAGDTNIKYLTDHGVTIW
DEWADENGDLGPVYGRQWRSWPDGHGGSIDQIAGLLKEIRRNPQSRRLIVSAWNPAEVEAMALPPCHCLFQFYVSEGRLS
CQLYQRSADIFLGVPFNIASYALLTLMVAQVTGLKPGDFVHTLGDAHLYSNHFEQAREQLQRTPRALPTMWINPEVKDLF
AFRFEDFRLENYVADASIQAPIAV

Sequences:

>Translated_264_residues
MRQYLDLLQHVLDNGADRSDRTGTGTRSVFGYQMRFDLARGFPVTTTKKLHLKSIIHELLWFLAGDTNIKYLTDHGVTIW
DEWADENGDLGPVYGRQWRSWPDGHGGSIDQIAGLLKEIRRNPQSRRLIVSAWNPAEVEAMALPPCHCLFQFYVSEGRLS
CQLYQRSADIFLGVPFNIASYALLTLMVAQVTGLKPGDFVHTLGDAHLYSNHFEQAREQLQRTPRALPTMWINPEVKDLF
AFRFEDFRLENYVADASIQAPIAV
>Mature_264_residues
MRQYLDLLQHVLDNGADRSDRTGTGTRSVFGYQMRFDLARGFPVTTTKKLHLKSIIHELLWFLAGDTNIKYLTDHGVTIW
DEWADENGDLGPVYGRQWRSWPDGHGGSIDQIAGLLKEIRRNPQSRRLIVSAWNPAEVEAMALPPCHCLFQFYVSEGRLS
CQLYQRSADIFLGVPFNIASYALLTLMVAQVTGLKPGDFVHTLGDAHLYSNHFEQAREQLQRTPRALPTMWINPEVKDLF
AFRFEDFRLENYVADASIQAPIAV

Specific function: Provides the sole de novo source of dTMP for DNA biosynthesis

COG id: COG0207

COG function: function code F; Thymidylate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate synthase family. Bacterial- type thyA subfamily

Homologues:

Organism=Homo sapiens, GI4507751, Length=282, Percent_Identity=50.709219858156, Blast_Score=283, Evalue=8e-77,
Organism=Escherichia coli, GI1789191, Length=264, Percent_Identity=62.1212121212121, Blast_Score=357, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI71993377, Length=283, Percent_Identity=46.2897526501767, Blast_Score=259, Evalue=1e-69,
Organism=Saccharomyces cerevisiae, GI6324648, Length=291, Percent_Identity=41.5807560137457, Blast_Score=211, Evalue=8e-56,
Organism=Drosophila melanogaster, GI17137556, Length=284, Percent_Identity=54.9295774647887, Blast_Score=306, Evalue=7e-84,

Paralogues:

None

Copy number: 391 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): TYSY_RHILO (Q98KH9)

Other databases:

- EMBL:   BA000012
- RefSeq:   NP_103049.1
- ProteinModelPortal:   Q98KH9
- SMR:   Q98KH9
- GeneID:   1225712
- GenomeReviews:   BA000012_GR
- KEGG:   mlo:mll1467
- NMPDR:   fig|266835.1.peg.1154
- HOGENOM:   HBG588098
- OMA:   RMALAPC
- ProtClustDB:   PRK01827
- BRENDA:   2.1.1.45
- GO:   GO:0005737
- HAMAP:   MF_00008
- InterPro:   IPR000398
- InterPro:   IPR020940
- Gene3D:   G3DSA:3.30.572.10
- PANTHER:   PTHR11549:SF2
- PRINTS:   PR00108
- TIGRFAMs:   TIGR03284

Pfam domain/function: PF00303 Thymidylat_synt; SSF55831 Thymidylat_synth_C

EC number: =2.1.1.45

Molecular weight: Translated: 30097; Mature: 30097

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: PS00091 THYMIDYLATE_SYNTHASE

Important sites: ACT_SITE 146-146

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRQYLDLLQHVLDNGADRSDRTGTGTRSVFGYQMRFDLARGFPVTTTKKLHLKSIIHELL
CHHHHHHHHHHHHCCCCCCCCCCCCCHHHEEHHEEEHHHCCCCCCCCHHHHHHHHHHHHH
WFLAGDTNIKYLTDHGVTIWDEWADENGDLGPVYGRQWRSWPDGHGGSIDQIAGLLKEIR
HHHCCCCCEEEEECCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
RNPQSRRLIVSAWNPAEVEAMALPPCHCLFQFYVSEGRLSCQLYQRSADIFLGVPFNIAS
CCCCCCEEEEEECCCCCEEEEECCHHHHHHHHHHHCCCEEEEEEECCCCEEEECCHHHHH
YALLTLMVAQVTGLKPGDFVHTLGDAHLYSNHFEQAREQLQRTPRALPTMWINPEVKDLF
HHHHHHHHHHHHCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHCHHHCCEEECCCCHHHHH
AFRFEDFRLENYVADASIQAPIAV
HHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MRQYLDLLQHVLDNGADRSDRTGTGTRSVFGYQMRFDLARGFPVTTTKKLHLKSIIHELL
CHHHHHHHHHHHHCCCCCCCCCCCCCHHHEEHHEEEHHHCCCCCCCCHHHHHHHHHHHHH
WFLAGDTNIKYLTDHGVTIWDEWADENGDLGPVYGRQWRSWPDGHGGSIDQIAGLLKEIR
HHHCCCCCEEEEECCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
RNPQSRRLIVSAWNPAEVEAMALPPCHCLFQFYVSEGRLSCQLYQRSADIFLGVPFNIAS
CCCCCCEEEEEECCCCCEEEEECCHHHHHHHHHHHCCCEEEEEEECCCCEEEECCHHHHH
YALLTLMVAQVTGLKPGDFVHTLGDAHLYSNHFEQAREQLQRTPRALPTMWINPEVKDLF
HHHHHHHHHHHHCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHCHHHCCEEECCCCHHHHH
AFRFEDFRLENYVADASIQAPIAV
HHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11214968